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Influenza virus vaccine expressing fusion and attachment protein epitopes of respiratory syncytial virus induces protective antibodies

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Influenza virus vaccine expressing fusion and attachment pro...

第十二届全国免疫学学术大会

作者： Wang Xiliang Gu Hongjing Wang Keyu Zhou Shanshan penghui yang Beijing Institute of Microbiology and Epidemiology State Key Laboratory of Pathogen and Biosecurity Beijing 302 Hospital

Respiratory syncytial virus(RSV) is an important viral pathogen that causes life-threatening respiratory infections in both infants and the elderly;no vaccines are at present *** this report,we examined the use of influenza virus as a vehicle for production of an experimental RSV *** used reverse genetics to generate a recombinant influenza A virus with epitopes from the RSV fusion(F) and attachment(G) proteins in the cold-adapted influenza virus NA *** of RSV F+G epitope proteins was confirmed by Western blotting,and no changes in viral morphology were evident following examination by electron *** rat immunized intranasally with rFlu/RSV/F+G showed viral-specific antibody responses against both influenza and *** IgG,IgG1,IgG2 a and IgA were measured in mice immunized with rFlu/RSV/F+G,revealing robust cellular and mucosal immune ***,we found that rFlu/RSV/F+G conferred protection against subsequent influenza and RSV challenges,showing significant decreases in viral replication and obvious attenuation of histopathological changes associated with viral *** findings suggest that rFlu/RSV/F+G is a promising vaccine candidate,which should be further assessed using primate models.

关键词： RSV fusion protein F and attachment(G) proteins Cold-adapted influenza virus

Oncolytic activity of a chimeric influenza A virus carrying human CTLA4 antibody in hepatocellular carcinoma

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Oncolytic activity of a chimeric influenza A virus carrying ...

第十四届全国免疫学学术大会

作者： Hao yang Guanglin Lei Fang Sun Jinxia Cheng Jin Yan Shaogeng Zhang penghui yang The Fifth Medical Center of Chinese PLA General Hospital Hebei North University

Oncolytic virotherapy belongs to a kind of active immunotherapy, which could trigger anti-tumor immune response through the dual mechanisms of selective tumor-killing without destroying normal tissues and induction of systemic anti-tumor immunity. In this study, we successfully rescued a chimeric oncolytic virus expressing human CTLA4 antibody in the Background of the A/PuertoRico/8/34(PR8) influenza virus. The chimeric virus, called rFlu-huCTLA4, contained the heavy and light chains of human CTLA4 antibody in the PB1 and PA segments of the PR8 virus, respectively. The first-generation hemagglutination(HA) titers of the rFlu-huCTLA4 virus ranged from 27 to 28, which could be passaged stably in specific pathogen-free(SPF) chicken embryos from P1 to P5. The morphology and size distribution of the chimeric virus was consistent with that of the wild-type influenza virus. rFlu-huCTLA4 virus could effectively replicate in various cells in a time and dose-dependent manner. ELISA assay revealed that the secreted huCTLA4 antibody levels in chicken embryos increased gradually over time. Furthermore, MTS and crystal violet analysis showed that the selective cytotoxicity of the virus was higher in hepatocellular cancer cells(HepG2 and Huh7) than in normal liver cells(MIHA) in vitro. More importantly, in a human liver cancer patient-derived xenograft model(PDX), intratumoral injections with rFlu-huCTLA4 could significantly inhibit the growth of the tumor as indicated by tumor volume, viral titers, and pathology changes. Taken together, these Results demonstrated that the chimeric influenza virus rFlu-huCTLA4 could selectively destroy hepatoma cells in vitro and in vivo, and may provide a promising clinical strategy for targeted therapy of HCC with the oncolytic flu virus.

关键词： Oncolytic virus CTLA4 antibody HCC

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CADI冲击疲劳失效机理研究

机械工程学报 2023年第22期59卷 312-321页

作者：杨鹏辉符寒光徐建林兰州理工大学省部共建有色金属先进加工与再利用国家重点实验室兰州730050 兰州理工大学材料科学与工程学院兰州730050 北京工业大学材料科学与工程学院北京100124

含碳化物等温淬火球墨铸铁(Carbidic austempered ductile iron,CADI)因其优良力学性能已成为球磨机磨球的重要材料之一,然而由于CADI组相复杂,导致目前研究无法搞清楚石墨球、铁素体、残余奥氏体和碳化物如何影响冲击疲劳下CADI的失效... 详细信息

含碳化物等温淬火球墨铸铁(Carbidic austempered ductile iron,CADI)因其优良力学性能已成为球磨机磨球的重要材料之一,然而由于CADI组相复杂,导致目前研究无法搞清楚石墨球、铁素体、残余奥氏体和碳化物如何影响冲击疲劳下CADI的失效。使用改造后的MLD-10试验机分析奥氏体化温度、等温淬火温度及冲击载荷对CADI冲击疲劳裂纹影响。通过自制试验机制备冲击疲劳试样,研究冲击疲劳后试样表面和横截面的变化规律,并揭示CADI冲击疲劳失效机理,即石墨球变形和碳化物断裂产生了大量冲击疲劳裂纹,导致CADI沿晶断裂。细小且分布均匀的石墨球和碳化物更有利于提高CADI抗冲击疲劳性,超高温预热处理+等温淬火的工艺获得的含沉淀颗粒和超细针状铁素体的高韧性CADI不易在冲击疲劳过程中断裂,具有广阔的应用前景。

关键词： CADI 冲击疲劳组织失效机理

8,10-二氢-7H-4b,8,10-三氮杂苯并薁衍生物的合成

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化学通报 2009年第12期72卷 1129-1131页

作者：杨鹏辉张群正西安石油大学化学化工学院西安710065

苯并咪唑类化合物具有广泛的生物活性,其合成和应用研究一直十分活跃。以2-氨甲基苯并咪唑二盐酸盐与α,β-不饱和酮在三乙胺存在下,以正丁醇为溶剂反应,合成了4种8,10-二氢-7H-4b,8,10-三氮杂苯并薁类衍生物,产率61%～67%。反应产物结... 详细信息

苯并咪唑类化合物具有广泛的生物活性,其合成和应用研究一直十分活跃。以2-氨甲基苯并咪唑二盐酸盐与α,β-不饱和酮在三乙胺存在下,以正丁醇为溶剂反应,合成了4种8,10-二氢-7H-4b,8,10-三氮杂苯并薁类衍生物,产率61%～67%。反应产物结构通过NMR、MS、元素分析和IR表征。

关键词： 2-氨甲基苯并咪唑二盐酸盐苯并薁合成

新型苯并咪唑取代的丙烯腈及丙烯酸酯衍生物的催化合成

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化学通报 2015年第12期78卷 1170-1172页

作者：杨鹏辉孙伟西安石油大学化学化工学院西安710065 西北大学化学与材料科学学院合成与天然功能分子化学教育部重点实验室西安710069

从邻苯二胺出发,合成了2-氰甲基苯并咪唑,在优选出的催化剂吗啡啉/乙酸催化下,合成了新型的双苯并咪唑丙烯腈衍生物和3-吲哚-2-苯并咪唑丙烯腈衍生物;将2-氰甲基苯并咪唑经过碱性水解、酯化,得到苯并咪唑乙酸乙酯,后者经过Knoevenagel缩... 详细信息

从邻苯二胺出发,合成了2-氰甲基苯并咪唑,在优选出的催化剂吗啡啉/乙酸催化下,合成了新型的双苯并咪唑丙烯腈衍生物和3-吲哚-2-苯并咪唑丙烯腈衍生物;将2-氰甲基苯并咪唑经过碱性水解、酯化,得到苯并咪唑乙酸乙酯,后者经过Knoevenagel缩合/分子内酯交换接续反应高产率地得到了新型的苯并咪唑取代的香豆素衍生物。

关键词： 2-氰甲基苯并咪唑 Knoevenagel反应合成

Influenza virus vaccine expressing fusion and attachment protein epitopes of respiratory syncytial virus induces protective antibodies in vivo

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Influenza virus vaccine expressing fusion and attachment pro...

第九届全国免疫学学术大会

作者： penghui yang Chengrong Bian Yueqiang Duan Hongjing Gu Shaogeng Zhang Xiliang Wang Beijing Institute of Microbiology and Epidemiology State Key Laboratory of Pathogen and Biosecurity Beijing302 Hospital

关键词：

RC框架HPFRC耗能墙结构侧向刚度计算模型

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建筑材料学报 2020年第5期23卷 1071-1077页

作者：杨鹏辉梁兴文何伟辛力西安建筑科技大学建筑设计研究院陕西西安710055 西安建筑科技大学土木工程学院陕西西安710055 中国启源工程设计研究院有限公司陕西西安710018 中国建筑西北设计研究院有限公司陕西西安710018

研究了钢筋混凝土(RC)框架高性能纤维增强混凝土(HPFRC)耗能墙结构中RC框架和HPFRC耗能墙的刚度退化规律,建立了RC框架HPFRC耗能墙结构在开裂、屈服和峰值荷载点的侧向刚度计算模型.结果表明:RC框架与HPFRC耗能墙在同一特征荷载点的刚... 详细信息

研究了钢筋混凝土(RC)框架高性能纤维增强混凝土(HPFRC)耗能墙结构中RC框架和HPFRC耗能墙的刚度退化规律,建立了RC框架HPFRC耗能墙结构在开裂、屈服和峰值荷载点的侧向刚度计算模型.结果表明:RC框架与HPFRC耗能墙在同一特征荷载点的刚度退化规律差异较大,HPFRC耗能墙比RC框架的刚度退化缓慢很多;基于RC框架与HPFRC耗能墙的刚度退化规律所建立的有效侧向刚度计算模型,基本反映了RC框架HPFRC耗能墙结构的受力和变形特点,按此模型计算所得的结构位移值与试验值基本吻合.

关键词：钢筋混凝土框架高性能纤维增强混凝土耗能墙侧向刚度计算模型刚度退化规律结构位移

Decreased long intergenic noncoding RNA P7 predicts unfavorable prognosis and promotes tumor proliferation via the modulation of the STAT1-MAPK pathway in hepatocellular carcinoma

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Decreased long intergenic noncoding RNA P7 predicts unfavora...

第十二届全国免疫学学术大会

作者： yang penghui Cheng Sijie Wang Keyu Yan Jin Sun Fang Zhang Shaogeng Beijing 302 Hospital State Key Laboratory of Pathogens and Biosecurity Beijing Institute of Microbiology and Epidemiology

Hepatocellular carcinoma(HCC) is the most common neoplasm and is a leading cause of cancer-related *** advances in the diagnosis and management of HCC,its prognosisremain *** evidence has shown that long intergenic noncoding RNAs(lincRNAs) play central roles inthe development of *** this study,we identified a long intergenic noncoding KNA referred to as lincRNA P7 in HCC and explored its clinical significance and biological functions in *** expression level of lincRNA P7 was significantly aberrantly deceased in HCC cancer tissues and cells ***-and loss-of-function experiments revealed that overexpression of lincRNA P7 significantly inhibited the proliferation of HCC-derived cancer cells,whereas lincRNA P7 knockdown promoted cell ***,lincRNA P7 blocked Erk1/2 signaling and repressed activation of the STAT1 *** nude mouse models,we show that overexpression of lincRNA P7 effectively repressed HCC xenograft tumor growth in ***,a clinical investigation demonstrated that down-regulated lincRNA P7 expression correlated with liver cirrhosis,Hepatitis B virus(HBV)infection,clinical stage of the tumor and recurrence.A Kaplan-Meier survival analysis showed that the expression of lincRNA P7 was significantly related to overall survival(P=0.003) and recurrence-free survival(P=0.031).Collectively,our findings suggested that the down-regulation of lincRNA P7 predicts poor clinical outcomes for HCC patients and might be a powerful candidate prognostic biomarker and target in HCC.

关键词： HCC long noncoding RNA lincRNA P7

Ginsenoside Rg2 targets Cd150+Cd48+Vwf+ hematopoietic stem and progenitor cells for hematopoietic regeneration

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Ginsenoside Rg2 targets Cd150+Cd48+Vwf+ hematopoietic stem a...

浙江省免疫学会第十三次学术大会

作者： Jing Qian penghui yang Tingting Ye shiyu Zhang Xiaoyan Lu Xiaohui Fan Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang University

Background:Various bioactive compound derived from traditional Chinese herbal medicine could improve hematopoietic function during chemotherapy induced myelosuppression therefore may benifite the tumor bearing ***,the missing of molecular mechiansm of action limits their clinical *** the rapidly developing single-cell profiling technologies that offer opportunities for mapping the dynamic molecular changes underlying hematopoietic lineage differentiation,the verification of cell fate choices an individual cell level become ***:In our previous study,we revealed that ginsenoside Rg2（Rg2）,a chemical compound of GINSENG（renshen in Chinese） has pronounced bioactivities of protecting mice from 5-FU induced *** study aims to investigate the target of Rg2 at single-cell resolution by means of single-cell RNA sequencing（Sc-RNA seq）.Materials and methods:In addition to measuring the indicators for hematopoiesis including femoral histopathology,bone marrow nucleated cell（BMNC） count and hematopoietic progenitor cells（HPCs） colony-forming unit（CFU） assay.A portion of BMNCs were subjected to Sc-RNA seq using 10 x Genomics ChromiumTM system and Illumina ***:Biological study confirmed that Rg2 could significantly improve the bone marrow morphology,increase the percentage of HSCs and the quantities of BFU-E and CFU-GM in ***2 could stimulate the proliferation of HSC and the differentiation of HPC to all lineages,which may thereby accelerate the recovery of hematopoietic function in myelosuppressive *** to the atlas of bone marrow hematopoietic cells generated from Sc-RNA seq data,we observed a skewed distribution of hematopoietic cells in 5-FU treated mice,which is featured by almost diminished hematopoietic cells in the myeloid cell population,robust abundant number of dendritic cells,as well as imbalanced distribution of T,B,and NK cells in the lymphoid cell population（featured by skewed T/B p

关键词： myelosuppression ginsenoside Rg2 hematopoiesis single-cell RNA-seq