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Comparative plastomic analysis and insights into the phylogeny of Salvia (Lamiaceae)

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Plant Diversity 2021年第1期43卷 15-26页

作者： Hong Wu peng-fei ma Hong-Tao Li Guo-Xiong Hu De-Zhu Li Germplasm Bank of Wild Species Kunming Institute of BotanyChinese Academy of SciencesKunmingYunnan650201China University of Chinese Academy of Sciences Beijing100049China College of Life Sciences Guizhou UniversityGuiyangGuizhou550025China

Salvia is the largest genus of Lamiaceae,with almost 1000 species,and has been divided into 11 *** ***,native to East Asia,is particularly important because of its potential medicinal ***,the interspecific relationships of this subgenus have not been resolved and the plastomes of Salvia have rarely been *** the current study,we compared plastid genome structure and organization of 19 species of Salvia(14 newly sequenced and 5 previously published).Our comparative analysis showed that all Salvia plastomes examined have a quadripartite structure typical of most angiosperms and contain an identical set of 114 unique genes(80 protein-coding genes,4 rRNA genes,and 30 tRNA genes).The plastome structure of all Salvia species is highly conserved like other Lamiaceae *** content,gene order,and GC content were highly similar in these *** inverted repeats/single copy region(IR/SC)boundaries of Salvia are highly conserved,and IR contraction only occurred in two species(Salvia mekongensis and ***).In Salvia,sequence divergence was higher in non-coding regions than in coding *** found that using large single copy(LSC)and small single copy regions(SSC)with exclusion of the rapidly evolving sites produced the highest resolution in phylogenetic analysis of Salvia,suggesting that using suitable informative sites to build trees is more conducive in phylogenetic *** study assembled a powerful matrix data set for studying the phylogeny of Salvia,resolving the interspecific relationship of Salvia *** newly sequenced plastid genomes will also enrich the plastome database of Salvia,providing the scientific basis for the development and utilization of germplasm resources of this large and important genus.

关键词： Lamiaceae Salvia subg.Glutinaria Plastome Phylogeny

Testing complete plastomes and nuclear ribosomal DNA sequences for species identification in a taxonomically difficult bamboo genus Fargesia

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Plant Diversity 2023年第2期45卷 147-155页

作者： Shi-Yu Lv Xia-Ying Ye Zhong-Hu Li peng-fei ma De-Zhu Li School of Life Science Northwest UniversityXi'anShaanxi710069China Germplasm Bank of Wild Species Kunming Institute of BotanyChinese Academy of SciencesKunmingYunnan650201China Agronomy and Life Science Department Zhaotong UniversityZhaotongYunnan657000China Kunming College of Life Science University of Chinese Academy of SciencesKunmingYunnan650201China

Fargesia,the largest genus within the temperate bamboo tribe Arundinarieae,has more than 90 species mainly distributed in the mountains of Southwest *** Fargesia bamboos are important components of the subalpine forest ecosystems that provide food and habitat for many endangered animals,including the giant ***,species-level identification of Fargesia is ***,the rapid radiation and slow molecular evolutionary rate of Fargesia pose a significant challenge to using DNA barcoding with standard plant barcodes(rbcL,matK,and ITS) in *** progress in the sequencing technologies,complete plastid genomes(plastomes) and nuclear ribosomal DNA(nrDNA)sequences have been proposed as organelle barcodes for species identification;however,these have not been tested in *** collected 196 individuals representing 62 species of Fargesia to comprehensively evaluate the discriminatory power of plastomes and nrDNA sequences compared to standard *** analysis indicates that complete plastomes have substantially higher discriminatory power(28.6%) than standard barcodes(5.7%),whereas nrDNA sequences show a moderate improvement(65.4%) compared to ITS(47.2%).We also found that nuclear markers performed better than plastid markers,and ITS alone had higher discriminatory power than complete *** study also demonstrated that plastomes and nrDNA sequences can contribute to intrageneric phylogenetic resolution in ***,neither of these sequences were able to discriminate all the sampled species,and therefore,more nuclear markers need to be identified.

关键词： Fargesia Genome-skimming DNA barcoding Plastome Ribosomal DNA

Function of chloride intracellular channel 1 in gastric cancer cells

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World Journal of Gastroenterology 2012年第24期18卷 3070-3080页

作者： peng-fei ma Jun-Qiang Chen Zhen Wang Jin-Lu Liu Bo-Pei Li Department of Gastrointestinal Surgery the First Affili- ated Hospital of Guangxi Medical University Nanning 530021 Guangxi Zhuang Autonomous Region China

AIM:To investigate the effect of chloride intracellular channel 1(CLIC1) on the cell proliferation,apoptosis,migration and invasion of gastric cancer ***:CLIC1 expression was evaluated in human gastric cancer cell lines SGC-7901 and MGC-803 by real time polymerase chain reaction(RT-PCR).Four segments of small interference RNA(siRNA) targeting CLIC1 mRNA and a no-sense control segment were designed by bioinformatics ***1 siRNA was selected using Lipofectamine 2000 and transfected transiently into human gastric cancer SGC-7901 and MGC-803 *** transfected efficiency was observed under fluorescence *** transfection,mRNA expression of CLIC1 was detected with RT-PCR and Western blotting was used to detect the protein *** was examined by methyl thiazolyl tetrazolium and apoptosis was detected with flow *** membrane transwell chamber and matrigel were used for the detection of the changes of invasion and migration of the two cell ***:In gastric cancer cell lines SGC-7901 and MGC-803,CLIC1 was obviously expressed and CLIC1 siRNA could effectively suppress the expression of CLIC1 protein and *** of cells transfected with CLIC1 siRNA3 was enhanced notably,and the highest proliferation rate was 23.3%(P = 0.002) in SGC-7901 and 35.55%(P = 0.001) in MGC-803 cells at 48 *** G2/M phase proportion increased,while G0/G1 and S phase proportions *** apoptotic rate of the CLIC1 siRNA3 group obviously decreased in both SGC-7901 cells(62.24%,P = 0.000) and MGC-803 cells(52.67%,P = 0.004).Down-regulation of CLIC1 led to the inhibition of invasion and migration by 54.31%(P = 0.000) and 33.62%(P = 0.001) in SGC-7901 and 40.74%(P = 0.000) and 29.26%(P = 0.002) in ***,there was no significant difference between the mock group cells and the negative control group cells.

关键词： Chloride intracellular channel 1 Gastric car-cinoma Small interference RNA Apoptosis Invasion Migration

Binder jetting 3D printing process optimization for rapid casting of green parts with high tensile strength

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China Foundry 2021年第4期18卷 335-343页

作者： Zhao-fa Zhang Li Wang Lin-tao Zhang peng-fei ma Bing-heng Lu Chen-wei Du Micro and Nano Technology Research Center State Key Laboratory for Manufacturing Systems EngineeringXi’an Jiaotong UniversityXi’an 710049China National Innovation Institute of Additive manufacturing Xi’an 710065China Beijing Hangxing machinery manufacturing Co. Ltd.Beijing 100013China

Binder jetting 3D printing is a rapid,cost effective,and efficient moulding/core making process,which can be applied to a large variety of ***,it exhibits a relatively low green-part *** may cause the collapse of the printed parts during de-caking and the pick-up procedure,especially in the case of small-scale structures,such as thin walls,tips,and *** this work,polyvinyl alcohol(PVA)was used as the additive in coated sand *** exploiting the binding effect between the two composites(thermoplastic phenolic resin and PVA)triggered by the binder,bonding necks firmly form among the sand particles,improving the green-part strength of the coated sand printed *** based on the Taguchi method were used to investigate the relationship between the process parameters and the green-part tensile *** following set of optimal process parameters was identified:50wt.%alcoholicity of the binder,75%binder saturation,0.36 mm layer thickness and 4.5wt.%PVA ***,the effect of such parameters on the green-part tensile strength was determined via statistical *** green part of an engine cylinder head sand pattern with complex cavity structures was printed,and the green-part tensile strength reached 2.31 ***,the ZL301 aluminum alloy impeller shape casting was prepared using sand molds printed with the optimal process *** results confirm that the proposed binder jetting 3D printing process can guarantee the integrity of the printed green parts and of small-size structures during de-caking and the pick-up ***,the casting made from the printed sand molds exhibits a relatively high quality.

关键词： binder jetting green-part strength coated sand polyvinyl alcohol rapid casting

Fast charge transfer kinetics in Sv-ZnIn_(2)S_(4)/Sb_(2)S_(3)S-scheme heterojunction photocatalyst for enhanced photocatalytic hydrogen evolution

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Rare Metals 2024年第2期43卷 533-542页

作者： Wei Li Jia-Jun Li Zhi-fei Liu Hong-Yu ma peng-fei Fang Rui Xiong Jian-Hong Wei Key Laboratory of Artificial Micro-and Nano-Structures of Ministry of Education School of Physics and TechnologyWuhan UniversityWuhan430072China

Constructing a S-scheme heterojunction with tight interface contact and fast charge transfer is beneficial to improving the photocatalytic hydrogen evolution ***,a unique one-dimensional(1D)/two-dimensional(2D)S-scheme heterojunction containing 1D Sb_(2)S_(3) nanorods and 2D ZnIn_(2)S_(4) with affluent sulfur vacancies(denoted as Sv-ZnIn_(2)S_(4)@Sb_(2)S_(3)) was *** introduced sulfur vacancy can promote the effective adsorption of H+for the following interfacial hydrogen-evolution ***,the larger contact area and stronger electron interaction between Sb_(2)S_(3) and ZnIn_(2)S_(4) effectively inhibits the recombination of photo-generated electron–hole pairs and abridges the migration distance of *** a result,the optimal Sv-ZnIn_(2)S_(4)@Sb_(2)S_(3) sample achieves H_(2) evolution activity of 2741.3 mol·h^(−1)·g^(−1),which is 8.6 times that of pristine ZnIn_(2)S_(4) and 3.0 times that of the Sv-ZnIn_(2)S_(4) *** on the experimental result,the photo-reactivity S-scheme mechanism of hydrogen evolution from water splitting with Sv-ZnIn_(2)S_(4)@Sb_(2)S_(3) is *** work provides an effective method for developing S-scheme heterojunction composites of transition metal sulfide with high hydrogen evolution performance.

关键词： ZnIn_(2)S_(4) nanosheets S-scheme heterojunction Sulfur vacancy Charge transfer and separation Photocatalytic H_(2)evolution

Application of radioactive iodine-125 microparticles in hepatocellular carcinoma with portal vein embolus

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World Journal of Gastrointestinal Surgery 2024年第7期16卷 2023-2030页

作者： peng Meng Ji-peng ma Xiao-fei Huang Kang-Le Zhang The Fourth Department of Oncology Yantai Hospital of Traditional Chinese MedicineYantai 264001Shandong ProvinceChina Department of Medical Services Yantai Hospital of Traditional Chinese MedicineYantai 264001Shandong ProvinceChina Department of Hepatobiliary Surgery The First Affiliated Hospital of Sun Yatsen UniversityGuangzhou 510062Guangdong ProvinceChina The Third Department of Oncology Yantai Hospital of Traditional Chinese MedicineYantai 264001Shandong ProvinceChina

BACKGROUND Radioactive iodine-125(125I)microparticle therapy is a new type of internal radiation therapy that has shown unique advantages in the treatment of malignant tumors,especially hepatocellular *** with hepatocellular carcinoma frequently experience portal vein embolism,which exacerbates the difficulty and complexity of treatment.125I particles,used in local radiotherapy,can directly act on tumor tissue and reduce damage to surrounding healthy *** retrospective analysis,this study discussed the efficacy and safety of radioactive 125I particles in portal vein embolization patients with hepatocellular carcinoma in order to provide more powerful evidence supporting clinical *** To investigate the effect of transcatheter arterial chemoembolization combined with portal vein 125I particle implantation in the treatment of primary liver cancer patients with portal vein tumor thrombus and its influence on liver *** The clinical data of 96 patients with primary liver cancer combined with portal vein tumor thrombus admitted to our hospital between January 2020 and December 2023 were retrospectively ***-two patients received treatment with transcatheter arterial chemoembolization and implantation of 125I particles in the portal vein(combination group),while 44 patients received treatment with transcatheter arterial chemoembolization alone(control group).The therapeutic effects on tumor lesions,primary liver cancer,and portal vein tumor embolisms were compared between the two *** in relevant laboratory indexes before and after treatment were *** t test was used to compare the measurement data between the two groups,and the χ^(2) test was used to compare the counting data between *** The tumor lesion response rate in the combination group(59.62%vs 38.64%)and the response rate of patients with primary liver cancer complicated with portal vein tumor thrombus(80.77%vs 59.09%)were signi

关键词： Radioactive iodine-125 Hepatocellular carcinoma Transcatheter arterial chemoembolization Portal vein embolus Retrospective study

Improving corrosion resistance of additively manufactured WE43 magnesium alloy by high temperature oxidation for biodegradable applications

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Journal of magnesium and Alloys 2024年第3期12卷 940-953页

作者： Jinge Liu Bangzhao Yin fei Song Bingchuan Liu Bo peng peng Wen Yun Tian Yufeng Zheng Xiaolin ma Caimei Wang State Key Laboratory of Tribology in Advanced Equipment Beijing100084China Department of Mechanical Engineering Tsinghua UniversityBeijing 100084China Department of Orthopaedics Beijing Tsinghua Changgung HospitalInstitute for Precision MedicineTsinghua UniversityBeijing102218China Department of Orthopaedics Peking University Third HospitalBeijing 100191China Engineering Research Center of Bone and Joint Precision Medicine Ministry of EducationBeijing 100191China School of materials Science and Engineering Peking UniversityBeijing 100871China Beijing AKEC Medical Co. Ltd.Beijing 102200China

Laser powder bed fusion(L-PBF)has been employed to additively manufacture WE43 magnesium(Mg)alloy biodegradable implants,but WE43 L-PBF samples exhibit excessively rapid *** this work,dense WE43 L-PBF samples were built with the relativity density reaching 99.9%.High temperature oxidation was performed on the L-PBF samples in circulating air via various heating temperatures and holding *** oxidation and diffusion at the elevated temperature generated a gradient structure composed of an oxide layer at the surface,a transition layer in the middle and the *** oxide layer consisted of rare earth(RE)oxides,and became dense and thick with increasing the holding *** matrix was composed ofα-Mg,RE oxides and Mg_(24)RE_(5) *** precipitates almost disappeared in the transition *** passivation effect was observed in the samples treated by a suitable high temperature *** original L-PBF samples lost 40%weight after 3-day immersion in Hank’s solution,and broke into fragments after 7-day *** casted and solution treated samples lost roughly half of the weight after 28-day *** high temperature oxidation samples,which were heated at 525℃ for 8 h,kept the structural integrity,and lost only 6.88%weight after 28-day *** substantially improved corrosion resistance was contributed to the gradient structure at the *** one hand,the outmost dense layer of RE oxides isolated the corrosive medium;on the other hand,the transition layer considerably inhibited the corrosion owing to the lack of ***,high temperature oxidation provides an efficient,economic and safe approach to inhibit the corrosion of WE43 L-PBF samples,and has promising prospects for future clinical applications.

关键词： Laser powder bed fusion Biodegradable magnesium alloy High temperature oxidation Corrosion resistance WE43.

HIV-Specific IL-2^+ and/or IFN-γ^+ CD8^+ T Cell Reponses during Chronic HIV-1 Infection in Former Blood Donors

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Biomedical and Environmental Sciences 2010年第5期23卷 391-401页

作者： YAN-MENG FENG YAN-MIN WAN LIAN-XIN LIU CHAO QIU peng-fei ma HONG peng YU-HUA RUAN LI-FENG HAN KUN-XUE HONG HUI XING YI-MING SHAO State Key Laboratory of Virology Wuhan Institute of VirologyChinese Academy of SciencesWuhan 430071HubeiChina State Key Laboratory for Infectious Diseases Prevention and Control Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China State Key Laboratory for Infectious Diseases Prevention and Control National Center for AIDS/STD Control and PreventionChinese Center for Disease Control and PreventionBeijing 100050China Graduate School of the Chinese Academy of Sciences Beijing 100049China

Objective Conflicting data have been generated from previous studies to determine which kind of relationship exists between HIV-1 specific CD8 Tcell responses and HIV-1 viral load or CD4 count over the course of *** this study,153 HIV-1 infected LTNPs were enrolled to investigate the role of HIV-1 specific CD8 T-cell responses in chronic HIV-1 infection among HIV-1 infected former blood *** The patients were stratified into three groups according to CD4 count：CD4≥500 cells/μL;350 cells/μL≤CD4〈500 cells/μL;CD4〈350 cells/μ*** were isolated from the patients＇ anticoagulated blood ***-2 and IFN-γ secretions of CD 8 T cells against 17 HIV-1 consensus B full peptide pools were analyzed by using ICS *** An overall inverse correlation were observed between CD4 count and plasma viral *** no significant difference was observed during the comparisons of frequency/breadth of HIV-1 specific CD8 T cell responses,CD4 count stratification analysis showed that different correlation pattern existed in three strata：as for patients whose CD4 counts were less than 350 cells/μL,no significant correlations were identified between frequency/breadth of HIV-1 specific CD8 T cell responses and CD4 count/viral load;as for patients whose CD4 counts ranged from 350 cells /μL to 500 cells/μL,significant correlation was only observed between the response breadth of IL-2＋IFN-γ＋ CD8 T cells and CD4 count;however,as for patients whose CD4 counts were more than 500 cells/μL,direct correlations were identified between IL-2＋IFN-γ＋/IL-2＋/IFN-γ＋ CD8 T cells and viral load or CD4 *** Universal consistent inverse correlation was only indentified between CD4 count and viral *** relationship between HIV-1 specific CD8 T cell responses and CD4 count/viral load varied in different CD4 strata,which showed that better preserved CD4 T cells were correlated with better CD8 T cell functions.

关键词： HIV-1 subtype B' CD8 T cell response IFN-γ IL-2 ICS

Coupling of RANS and BEM Solvers for Simulation of Propeller Behind the Hull in Full Appended Model

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China Ocean Engineering 2024年第3期38卷 424-438页

作者： Saeed NAJAFI Mehdi POURMOSTAFA LIU peng-fei Iranian National Institute for Oceanography and Atmospheric Science(INIOAS) Tehran 14155-4781Iran Department of maritime Engineering Amir Kabir University of TechnologyTehran 15875-4413Iran marine Offshore and Subsea TechnologySchool of EngineeringNewcastle UniversityNewcastle upon TyneNE17RUUK

To design a propeller for ship power plant,the interaction between ship hull and propeller must be taken into *** main concern is to apply the wake effect of ship stern on the propeller *** this paper,a coupled BEM(Boundary Element Method)/RANS(Renolds-Averaged Navier−Stokes)solver is used to simulate propeller behind the hull in the self-propulsion *** motivation of this work is to develop a practical tool to design marine propulsion system without suffering long computational *** unsteady boundary element method which is also known as panel method is chosen to estimate the propeller *** wakes are treated using a time marching wake alignment ***,a RANS code coupled with VoF equation is developed to consider the ship motions and wake field effects in the problem.A coupling algorithm is developed to interchange ship wake field to the potential flow solver and propeller thrust to the RANS *** on the difference between hull resistance and the propeller thrust,a PI controller is developed to compute the propeller RPM in every time *** of the solver is carried out using the towing tank test report of a 50 m oceanography research *** factor and trust deduction coefficient are estimated ***,the wake rollup pattern of the propeller in open water is compared with the propeller in real wake field.

关键词： RANS BEM coupled method ship propeller self-propulsion wake sheet pattern