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OsFK1 encodes C-14 sterol reductase,which is involved in sterol biosynthesis and affects premature aging of leaves in rice

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The Crop Journal 2024年第4期12卷 1010-1021页

作者： Ping li Xia Zhang Wuzhong Yin Yang Shui Jie Zhang Nana Xu Dasong Bai Qingxiong Huang Yuanyuan li Pan Qi Xing li Qianlong li Shihong Yang Guotao Yang Hong Chen peng Qin Yungao Hu Youlin peng Rice Research Institute School of Life Science and EngineeringSouthwest University of Science and TechnologyMianyang 621010SichuanChina Hainan Yazhou Bay Seed Laboratory Sanya 572025HainanChina Rice Research Institute State Key Laboratory of Crop Gene Exploration and Utilization in Southwest ChinaSichuan Agricultural UniversityChengdu 610061SichuanChina Yazhouwan National Laboratory Sanya 572704HainanChina

The enzyme C-14 sterol reductase is involved in biosynthesis of brassinosteroids(BR)and sterols,as well as plant ***1,a member of the sterol biosynthesis pathway located in the endoplasmic reticulum(ER),encodes C-14 sterol ***,there is little research on the function of C-14 sterol reductase in *** with the wild type,an osfk1 mutant showed dwarf phenotype and premature aging in the second leaf during the trefoil stage,and abnormal development of leaf veins during the tillering *** osfk1 mutant showed signs of aberrant PCD,as evidenced by TUNEL *** suggested that high ROS buildup caused DNA damage and ROS-mediated cell death in the *** osfk1 mutant also showed decreased chlorophyll content and aberrant chloroplast *** of the osfk1 mutant allele revealed a non-synonymous G to A mutation in the final intron,leading to early ***,we identified the OsFK1 allele,cloned it by Mutmap sequencing,and verified it by ***-MS/MS assays demonstrated that the osfk1 mutation caused lower phytosterol *** findings showed that the OsFK1 allele encoding C-14 sterol reductase is involved in phytosterol biosynthesis and mediates normal development of rice plants.

关键词： Leaf development ROS Phytosterol biosynthesis Rice

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Assembly and co-occurrence patterns of rare and abundant bacterial sub-communities in rice rhizosphere soil under short-term nitrogen deep placement

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Journal of Integrative Agriculture 2021年第12期20卷 3299-3311页

作者： li Gui-long WU Meng li peng-fa WEI Shi-ping liU Jia JIANG Chun-yu liU Ming li Zhong-pei State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil ScienceChinese Academy of SciencesNanjing 210008P.R.China University of Chinese Academy of Sciences Beijing 100049P.R.China Soil and Fertilizer&Resources and Environment Institute Jiangxi Academy of Agricultural SciencesNanchang 330200P.R.China

Nitrogen(N)deep placement has been found to reduce N leaching and increase N use efficiency in paddy ***,relatively little is known how bacterial consortia,especially abundant and rare taxa,respond to N deep placement,which is critical for understanding the biodiversity and function of agricultural *** this study,lllumina sequencing and ecological models were conducted to examine the diversity patterns and underlying assembly mechanisms of abundant and rare taxa in rice rhizosphere soil under different N fertilization regimes at four rice growth stages in paddy *** results showed that abundant and rare bacteria had distinct distribution patterns in rhizosphere *** bacteria showed ubiquitous distribution;while rare taxa exhibited uneven distribution across all *** processes dominated community assembly of both abundant and rare bacteria,with dispersal limitation playing a more vital role in abundant bacteria,and undominated processes playing a more important role in rare *** N deep placement was associated with a greater influence of dispersal limitation than the broadcast N fertilizer(BN)and no N fertilizer(NN)treatments in abundant and rare taxa of rhizosphere soil;while greater contributions from homogenizing dispersal were observed for BN and NN in rare *** analysis indicated that abundant taxa with closer relationships were usually more likely to occupy the central position of the network than rare ***,most of the keystone species were rare taxa and might have played essential roles in maintaining the network ***,these findings highlighted that the ecological mechanisms and co-occurrence patterns of abundant and rare bacteria in rhizosphere soil under N deep placement.

关键词： rare bacteria community assembly network analysis co-occurrence patterns N deep placement

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Synthesis,Characterization and Thermal Property of the Aluminum Boroxine-linked Compound with N-aryl Substituted β-Diketiminato ligand

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Chinese Journal of Structural Chemistry 2015年第5期34卷 729-734页

作者： HAO peng-Fei YANG Jun-Juan YANG Zhi MA Xiao-li YANG Ying li Jia-Rong School of Chemical Engineering and Environment Beijing Institute of Technology School of Chemistry and Chemical Engineering Central South University

The boroxine-linked organic aluminum compound LAl[OB（3-C4H3O）]2（μ-O） was accomplished by reacting LAl H2 {L =（Z）-4-[（2,6-diisopropylphenyl）amino]pent-3-en-2-ylidene-2,6-diisopropylaniline} with furan-3-ylboronic acid in good *** title compound belongs to the orthorhombic system,space group Pnma with a = 10.9774（15）,b = 19.369（3）,c = 17.362（3） A,V = 3691.5（9） A^3,C37H47 Al B2N2O5,Mr = 648.37,Z = 4,Dc = 1.167 Mg/m^3,μ（Mo Kα） = 0.097 mm^–1,F（000） = 1384,S = 1.000,the final R = 0.0589 and w R = 0.1445 for 9138 observed reflections（I 〉 2σ（I）） and R = 0.0683 and w R = 0.1517 for all *** compound is an unique example of a spiro-centered aluminum atom,showing the inorganic Al O3B2 ring fused to the organic C3N2 *** was characterized by 1H NMR,IR,elemental analysis,and single-crystal X-ray structural ***,the compound was studied by TG analysis as well as DSC.

关键词： β-diketiminato ligand boroxine-linked aluminum compound thermal property

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Synthesis of L-Ascorbic Acid Lactone Derivatives

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Natural Products and Bioprospecting 2014年第3期4卷 181-188页

作者： li-Dong Shao Ya-Nan Wu Jun Xu Juan He Yu Zhao li-Yan peng Yan li Yu-Rong Yang cheng-Feng Xia Qin-Shi Zhao State Key Laboratory of Phytochemistry and Plant Resources in West China Kunming Institute of BotanyChinese Academy of SciencesKunming 650201China Graduate School of the Chinese Academy of Sciences University of Chinese Academy of SciencesBeijing 100049China

A small focused library which comprised of L-AA lactone derivatives was built with a facile *** reported method was optimized by modifying the acidity of the *** a result,12 L-AA lactones were *** these lactones,lacto... 详细信息

关键词： L-Ascorbic acid lactone Cytotoxicity Focused library

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THE WALL EFFECT ON VENTILATED CAVITATING FLOWS IN CLOSED CAVITATION TUNNELS

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Journal of Hydrodynamics 2008年第5期20卷 561-566页

作者： CHEN Xin LU Chuan-jing li Jie PAN Zhan-cheng Department of Engineering Mechanics Shanghai Jiao Tong University Shanghai 200240 China

For ventilated cavitating flows in a closed water tunnel, the wall effect may exert an important influence on cavity shape and hydrodynamics, An isotropic mixture multiphase model was established to study the wall effect based on the RANS equations, coupled with a natural cavitation model and the RNG k-ε turbulent model. The governing equations were discretized using the finite volume method and solved by the Gauss-Seidel linear equation solver on the basis of a segregation algorithm. The algebraic multigrid approach was carried through to accelerate the convergence of solution. The steady ventilated cavitating flows in water tunnels of different diameter were simulated for a conceptual underwater vehicle model which had a disk cavitator. It is found that the choked cavitation number derived is close to the approximate solution of natural cavitating flow for a 3-D disk. The critical ventilation rate falls with decreasing diameter of the water tunnel. However, the cavity size and drag coeflicient are rising with the decrease in tunnel diameter for the same ventilation rate, and the cavity size will be much different in water tunnels of different diameter even for the same ventilated cavitation number.

关键词： ventilated cavitation water tunnel choked cavitation number critical ventilation rate

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Chicken gga-miR-1306-5p targets Tollip and plays an important role in host response against Salmonella enteritidis infection

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Journal of Animal Science and Biotechnology 2019年第4期10卷 873-883页

作者： Weiwei Sun Ranran liu peng li Qinghe li Huanxian Cui Maiqing Zheng Jie Wen Guiping Zhao Institute of Animal Sciences Chinese Academy of Agricultural Sciences State Key Laboratory of Animal Nutrition

Background: Increasing evidence indicates that micro RNAs(mi RNAs) are involved in inflammatory response and immune regulation following pathogen invasion. The purpose of this study was to elucidate the roles played by Gallus gallus micro RNA-1306-5 p(gga-mi R-1306-5 p) in host responses against potential invasion by Salmonella enteritidis(SE) in chickens and the underlying ***: In present study, the expression levels of gga-mi R-1306-5 p were determined in both tissues and HD11 cells. The results showed that gga-mi R-1306-5 p was significantly increased following SE infection or lipopolysaccharide(LPS) stimulation. The dual luciferase reporter assay further validated that gga-mi R-1306-5 p targeted the Toll-interacting protein(Tollip), and thereby participated in the regulation of immune response against SE or LPS stimulation through binding with the 3′-untranslated region(3’UTR) of Tollip. Additionally, the expression of Tollip was significantly blocked by over-expressed gga-mi R-1306-5 p. The underlying mechanisms by which ggami R-1306-5 p modulated the production of pro-inflammatory cytokines were also investigated. Molecular biological assays demonstrated that overexpression of gga-mi R-1306-5 p promoted the production of pro-inflammatory mediators, including NF-κB, TNF-α, IL-6, and IL-1β, which produced effects similar to those of Tollip ***: Taken together, gga-mi R-1306-5 p induced by SE or LPS, regulates the immune response by inhibiting Tollip, which activates the production of inflammatory cytokines. This study has provided the first direct evidence that gga-mi R-1306-5 p targets Tollip, and is involved in the host response against SE.

关键词： gga-miR-1306-5p LPS Pro-inflammatory cytokine Salmonella enteritidis Tollip

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Effects of refluxate pH values on duodenogastroesophageal reflux-induced esophageal adenocarcinoma

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World Journal of Gastroenterology 2011年第25期17卷 3060-3065页

作者： peng cheng Jian-Sheng li Jun Gong lian-Feng Zhang Rong-Zhong Chen Department of Gastroenterology First Hospitalof Zhengzhou University Zhengzhou 450052 Henan ProvinceChina Department of Gastroenterology Second Hospitalof Xi＇an Jiao Tong University Xi＇an 450052 Shanxi ProvinceChina

AIM： To determine the effects of duodenogastric juice pH on the development of esophageal adenocarcinoma （EAC）. METHODS： An animal model of duodenogastroesophageal reflux was established using Sprague-Dawley （SD） rats undergoing esophagoduodenostomy （ED）. The development of EAC was investigated in rats exposed to duodenogastric juice of different pH. The rats were di- vided into three groups： Iow-pH group （group A）, high- pH group （group B） and a sham-operated group as a control （group C）（n = 30 rats in each group）. The inci- dence of esophagitis, Barrett＇s esophagus （BE）, intestinal metaplasia with dysplasia and EAC was observed 40 wk after the treatment. RESULTS： The incidence rate of esophagitis, BE, intestinal metaplasia with dysplasia and EAC was higher in groups A and B compared with the control group after 40 wk （P 〈 0.01）, being 96% and 100% （P 〉 0.05）, 88% and 82.4% （P 〉 0.05）, 20% and 52.1% （P 〈 0.05）, and 8% and 39% （P 〈 0.05）, respectively. CONCLUSION： Non-acidic refluxate increases the occurrence of intestinal metaplasia with dysplasia and EAC while the Iow-pH gastric juice exerts a protective effect in the presence of duodenal juice. The non-acid reflux is particularly important in the progression from BE to cancer. Therefore, control of duodenal reflux may be an important prophylaxis for EAC.

关键词： Esophageal reflux Esophageal adenocar-cinoma pH-metry Pathogenesis

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Advances in the Development of Gradient Scaffolds Made of Nano-Micromaterials for Musculoskeletal Tissue Regeneration

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NANO-MICRO LETTERS 2025年第1期17卷 1-46页

作者： Fang, Lei lin, Xiaoqi Xu, Ruian liu, Lu Zhang, Yu Tian, Feng li, Jiao Jiao Xue, Jiajia Beijing Univ Chem Technol Beijing Lab Biomed Mat Beijing 100029 Peoples R China Beijing Univ Chem Technol State Key Lab Organ Inorgan Composites Beijing 100029 Peoples R China Univ Technol Sydney Fac Engn & IT Sch Biomed Engn Sydney NSW 2007 Australia

This review highlights the gradient variations in the structural composition of musculoskeletal tissues and comprehensively examines recent progress in the fabrication and application of biomimetic gradient scaffolds for musculoskeletal *** challenges and prospects of gradient scaffolds for clinical application are discussed. The intricate hierarchical structure of musculoskeletal tissues, including bone and interface tissues, necessitates the use of complex scaffold designs and material structures to serve as tissue-engineered substitutes. This has led to growing interest in the development of gradient bone scaffolds with hierarchical structures mimicking the extracellular matrix of native tissues to achieve improved therapeutic outcomes. Building on the anatomical characteristics of bone and interfacial tissues, this review provides a summary of current strategies used to design and fabricate biomimetic gradient scaffolds for repairing musculoskeletal tissues, specifically focusing on methods used to construct compositional and structural gradients within the scaffolds. The latest applications of gradient scaffolds for the regeneration of bone, osteochondral, and tendon-to-bone interfaces are presented. Furthermore, the current progress of testing gradient scaffolds in physiologically relevant animal models of skeletal repair is discussed, as well as the challenges and prospects of moving these scaffolds into clinical application for treating musculoskeletal injuries.

关键词： Gradient scaffolds Musculoskeletal tissues Advanced manufacturing Biomaterials Tissue regeneration

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Idiopathic colopleural fistula presenting with lung abscess and refractory empyema:A case report

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World Journal of Gastrointestinal Surgery 2023年第4期15卷 740-744页

作者： Chun-li Wang Kung-Chuan cheng Division of Colorectal Surgery Department of SurgeryKaohsiung Chang Gung Memorial HospitalKaohsiung 833Taiwan

BACKGROUND Colopleural fistula is a rare condition,and only a limited number of cases have been ***,we report a case of idiopathic colopleural fistula in an adult without any known predisposing *** patient presented with a lung abscess and refractory empyema and was successfully treated with surgical *** SUMMARY A 47-year-old man with a history of lung tuberculosis,which had been completely cured 4 years ago,presented to our emergency department with a productive cough and fever for 3 *** his history,he had undergone left lower lobe segmentectomy of the left lung due to lung abscess one year ago at another ***,he developed refractory empyema postoperatively despite surgical intervention including decortication and flap *** admission,we reviewed his previous medical images and noted a fistula tract between the left pleural cavity and splenic *** addition,according to his medical records,bacterial culture of the thoracic drainage showed growth of Escherichia coli and Bacteroides *** lower gastrointestinal series and colonoscopy confirmed the diagnosis of colopleural *** patient underwent a left hemicolectomy,splenectomy,and distal pancreatectomy,and the diaphragm was repaired under our *** further empyema recurrence was noted during *** Indicative signs of colopleural fistula include refractory empyema accompanied by the growth of colonic flora in the pleural fluid.

关键词： Colopleural fistula Lung abscess Empyema Colonic flora Case report

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Research status and performance optimization of medium-temperature thermoelectric material SnTe

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Chinese Physics B 2022年第4期31卷 18-30页

作者： Pan-Pan peng Chao Wang Lan-Wei li Shu-Yao li Yan-Qun Chen Institute for Computational Materials Science School of Physics and ElectronicsHenan UniversityKaifeng 475004China

Thermoelectric materials have the ability to directly convert heat into electricity,which have been extensively studied for decades to solve global energy shortages and environmental *** a medium temperature(400-800 K)thermoelectric material,SnTe has attracted extensive attention as a promising substitute for PbTe due to its non-toxic *** this paper,the research status of SnTe thermoelectric materials is reviewed,and the strategies to improve its performance are summarized and discussed in terms of electrical and thermal transport *** comprehensive discussion will provides guidance and inspiration for the research on SnTe.

关键词： SnTe thermoelectric materials electronic transport thermal conductivity

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