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The response of structure and nitrogen removal function of the biofilm on submerged macrophytes to high ammonium in constructed wetlands

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Journal of Environmental Sciences 2024年第8期142卷 129-141页

作者： Xue Jiang Mengmeng Wang Shunqing Yang Di He fei fang Liuyan Yang State Key Laboratory of Pollution Control and Resource Reuse School of the EnvironmentNanjing UniversityNanjing 210023China School of Resources and Environment Anqing Normal UniversityAnqing 246133China

The ammonium exceedance discharge from sewage treatment plants has a great risk to the stable operation of subsequent constructed wetlands(CWs).The effects of high ammonium shocks on submerged macrophytes and epiphytic biofilms on the leaves of submerged macrophytes in CWs were rarely mentioned in previous *** this paper,the 16S rRNA sequencing method was used to investigate the variation of the microbial communities in biofilms on the leaves of Vallisneria natans plants while the growth characteristics of *** plants were measured at different initial ammonium *** results demonstrated that the total chlorophyll and soluble sugar synthesis of *** plants decreased by 51.45%and 57.16%,respectively,and malondialdehyde content increased threefold after8 days if the initial NH_(4)^(+)-N concentration was more than 5 mg/*** density,bacterial quantity,dissolved oxygen,and pH increased with high ammonium *** average removal efficiencies of total nitrogen and NH_(4)^(+)-N reached 73.26%and 83.94%,*** heat map and relative abundance analysis represented that the relative abundances of phyla Proteobacteria,Cyanobacteria,and Bacteroidetes *** numbers of autotrophic nitrifiers and heterotrophic nitrification aerobic denitrification(HNAD)bacteria expanded in *** particular,HNAD bacteria of Flavobacterium,Hydrogenophaga,Acidovorax,Acinetobacter,Pseudomonas,Aeromonas,and Azospira had higher abundances than autotrophic nitrifiers because there were organic matters secreted from declining leaves of *** *** analysis of the nitrogen metabolic pathway showed aerobic denitrification was the main nitrogen removal ***,the nitrification and denitrification bacterial communities increased in epiphytic biofilms on submerged macrophytes in constructed wetlands while submerged macrophytes declined under ammonium shock loading.

关键词： Ammonium shock loading Biofilm Autotrophic nitrifiers Heterotrophic nitrification aerobic denitrification bacteria Submerged macrophyte

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Turnip mosaic virus pathogenesis and host resistance mechanisms in Brassica

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Horticultural Plant Journal 2024年第4期10卷 947-960页

作者： Guanwei Wu Xinxin fang Tianqi Yu Jianping Chen fei Yan State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products Institute of Plant VirologyNingbo UniversityNingboZhejiang 315211China Key Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province Institute of Plant VirologyNingbo UniversityNingboZhejiang 315211China

Turnip mosaic virus(TuMV)is a devastating potyvirus pathogen that infects a wide variety of both cultivated and wild Brassicaceae *** urgently need more information and understanding of TuMV pathogenesis and the host responses involved in disease development in cruciferous *** displays great versatility in viral pathogenesis,especially in its replication and intercellular ***,in the coevolutionary arms races between TuMV and its hosts,the virus has evolved to co-opt host factors to facilitate its infection and counter host defense *** review mainly focuses on recent advances in understanding the viral factors that contribute to the TuMV infection cycle and the host resistance mechanism in ***,we propose some future research directions on TuMV pathogenesis and control strategies to design durable TuMV-resistant Brassica crops.

关键词： Turnip mosaic virus Brassica Resistance genes Host factors Infection biology

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Fast charge transfer kinetics in Sv-ZnIn_(2)S_(4)/Sb_(2)S_(3)S-scheme heterojunction photocatalyst for enhanced photocatalytic hydrogen evolution

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Rare Metals 2024年第2期43卷 533-542页

作者： Wei Li Jia-Jun Li Zhi-fei Liu Hong-Yu Ma pen.-fei fang Rui Xiong Jian-Hong Wei Key Laboratory of Artificial Micro-and Nano-Structures of Ministry of Education School of Physics and TechnologyWuhan UniversityWuhan430072China

Constructing a S-scheme heterojunction with tight interface contact and fast charge transfer is beneficial to improving the photocatalytic hydrogen evolution ***,a unique one-dimensional(1D)/two-dimensional(2D)S-scheme heterojunction containing 1D Sb_(2)S_(3) nanorods and 2D ZnIn_(2)S_(4) with affluent sulfur vacancies(denoted as Sv-ZnIn_(2)S_(4)@Sb_(2)S_(3)) was *** introduced sulfur vacancy can promote the effective adsorption of H+for the following interfacial hydrogen-evolution ***,the larger contact area and stronger electron interaction between Sb_(2)S_(3) and ZnIn_(2)S_(4) effectively inhibits the recombination of photo-generated electron–hole pairs and abridges the migration distance of *** a result,the optimal Sv-ZnIn_(2)S_(4)@Sb_(2)S_(3) sample achieves H_(2) evolution activity of 2741.3 mol·h^(−1)·g^(−1),which is 8.6 times that of pristine ZnIn_(2)S_(4) and 3.0 times that of the Sv-ZnIn_(2)S_(4) *** on the experimental result,the photo-reactivity S-scheme mechanism of hydrogen evolution from water splitting with Sv-ZnIn_(2)S_(4)@Sb_(2)S_(3) is *** work provides an effective method for developing S-scheme heterojunction composites of transition metal sulfide with high hydrogen evolution performance.

关键词： ZnIn_(2)S_(4) nanosheets S-scheme heterojunction Sulfur vacancy Charge transfer and separation Photocatalytic H_(2)evolution

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Experimental and first-principles calculation of TiN growth mechanism on CeAlO_(3) surface in steel

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Journal of Iron and Steel Research International 2024年第2期31卷 452-463页

作者： fei-hu He Jun pen. fang Zhang Yong-bin Wang Hong-tao Chang School of Materials and Metallurgy Inner Mongolia University of Science and TechnologyBaotou014010Inner MongoliaChina

Regular TiN is harmful to the toughness of steel,and its shape and size need to be *** the behavior of TiN precipitation on CeAlO_(3) surfaces is critical for controlling the morphology and formation process of CeAlO_(3)–TiN composite inclusions in the *** results showed that TiN had a square morphology on the CeAlO3 surface,and electron backscatter diffraction phase identification results revealed the orientation relationship between CeAlO_(3) and TiN as follows:(001)_(CeAlO_(3))//(110)_(TiN),(100)_(CeAlO_(3))/(001)_(TiN),and[010]_(CeAlO_(3))/[110]_(TiN).The CeAlO3 crystal structure was studied using the first-principles calculation method,and the adsorption and growth process of TiN on the CeAlO_(3) surface were investigated from the atomic *** calculation results indicate that there is no metallic bonding present in the CeAlO_(3) *** the low-index crystal planes of CeAlO_(3),the(110)planes terminated with O and CeAlO have the highest and lowest surface energies,respectively,with values of 0.373 and 0.051 eV/Å^(2).On the high surface energy plane of CeAlO_(3),the TiN atomic permutation structure is consistent with the arrangement of Ti and N atoms in TiN(100)or(110).For the low surface energy plane of CeAlO_(3),the Ti and N atoms are arranged in a ring-like structure.

关键词： CeAlO_(3)-TiN inclusion Orientation relationship Crystal structure First principle Surface energy

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Dynamic Effect of Landslides Triggered by Earthquake:A Case Study in Moxi Town of Luding County,China

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Journal of Earth Science 2024年第1期35卷 221-234页

作者： Hongfu Zhou fei Ye Wenxi Fu Bin Liu Tian fang Rui Li Chengdu Center China Geological SurveyChengdu 610081China State Key Laboratory of Hydraulics and Mountain Rivers Protection Sichuan UniversityChengdu 610065China Sichuan Geological Survey Chengdu610081China Shandong University of Science and Technology Qingdao 266590China

The dynamic effect is a very important issue widely debated by scholars when studying the genetic and disaster-causing mechanisms of earthquake-triggered ***,the dynamic effect mechanism and phenomena of earthquake-triggered landslides were summarized in this ***,the primary types of dynamic effects were further used to interpret the Mogangling landslide in Moxi Town of Luding County,China.A field investigation,remote sensing,numerical calculation and theoretical analysis were carried out to illustrate the failure mechanism of slope rock masses affected by *** interaction between seismic waves and slope rock masses and the induced dynamic effect of slope rock masses were primarily accounted for in the *** slope topography,rock mass weathering and unloading characteristics,river erosion,regional seismogenic structure,and rock mass structure characteristics were also *** results showed that the formation of the Mogangling landslide was mainly related to the high amplification effect of seismic acceleration and back slope effects,interface dynamic stress effects,and double-sided slope effects of seismic waves caused by the catastrophic Ms 7.75 Moxi Earthquake in *** principles for the site and route selection of large-scale infrastructure in the planning stage and the scientific prevention of seismic geological disasters were proposed on the basis of the dynamic effect of earthquake-induced landslides.

关键词： dynamic effect coupling of endogenic and exogenic processes landslides earthquakes disaster prevention

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Machine learning and bioinformatics to identify biomarkers in response to Burkholderia pseudomallei infection in mice

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BIOCELL 2024年第4期48卷 613-621页

作者： YAO fang fei XIA feifei TIAN L EI QU fang YANG JUAN fang ZHENHONG HU HAICHAO LIU Department of Respiratory and Critical Care Medicine General Hospital of Center Theater of PLAWuhan430070China School of Medicine Wuhan University of Science and TechnologyWuhan430065China School of Life Science and Engineering Southwest Jiaotong UniversityChengdu611756China

Objective:In the realm of Class I pathogens,Burkholderia pseudomallei(BP)stands out for its propen.ity to induce severe *** the intricate interactions between BP and host cells is imperative for comprehending the dynamics of BP infection and discerning biomarkers indicative of the host cell response ***:mRNA extraction from BP-infected mouse macrophages constituted the initial step of our *** gene expression arrays,the extracted RNA underwent conversion into digital *** percentile shift method facilitated data processing,with the identiﬁcation of genes manifesting signiﬁcant differences accomplished through the application of the ***,a comprehensive analysis involving Gene Ontology enrichment and Kyoto Encyclopedia of Genes and Genomes pathway was conducted on the differentially expressed genes(DEGs).Leveraging the ESTIMATE algorithm,gene signatures were utilized to compute risk scores for gene expression *** vector machine analysis and gene enrichment scores were instrumental in establishing correlations between biomarkers and macrophages,followed by an evaluation of the predictive power of the identiﬁed ***:The functional and pathway associations of the DEGs predominantly centered around G protein-coupled receptors.A noteworthy positive correlation emerged between the blue module,consisting of 416 genes,and the ***4,identiﬁed through support vector machine analysis among intersecting genes,indicated a robust interaction with macrophages,suggesting its potential as a robust ***4 exhibited commendable predictive efﬁcacy,with BP infection inducing its expression in both macrophages and mouse lung *** blotting in macrophages conﬁrmed a signiﬁcant upregulation of FZD4 expression from 0.5 to 24 h *** mouse lung tissue,FZD4 manifested higher expression in the cytoplasm of pulmonary epithelial cells in BP-infected lungs than in the

关键词： Burkholderia pseudomallei Microarray assay Machine learning Bioinformatics FZD4

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Evaluating serum CXCL12,sCD22,Lp-PLA2 levels and ratios as biomarkers for diagnosis of Alzheimer's disease

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World Journal of Psychiatry 2024年第3期14卷 380-387页

作者： Zeng-Ling Liu fei-fei Hua Lei Qu Na Yan Hui-fang Zhang Department of Neurology Dongying People's HospitalDongying 257000Shandong ProvinceChina

BACKGROUND Grasping the underlying mechanisms of Alzheimer's disease(AD)is still a work in progress,and existing diagnostic techniques encounter various ***,the discovery of depen.able biomarkers is essential for early detection,tracking the disease's advancement,and steering treatment *** To explore the diagnostic potential of serum CXCL12,sCD22,Lp-PLA2,and their ratios in AD,aiming to enhance early detection and inform targeted treatment *** The study was conducted in Dongying people's Hospital from January 2021 to December *** included 60 AD patients(AD group)and 60 healthy people(control group).Using a prospective case-control design,the levels of CXCL12,sCD22 and Lp-PLA2 and their ratios were detected by enzyme-linked immunosorbent assay kit in the diagnosis of *** differences between the two groups were analyzed by statistical methods,and the corresponding ratio was constructed to improve the specificity and sensitivity of *** Serum CXCL12 levels were higher in the AD group(47.2±8.5 ng/mL)than the control group(32.8±5.7 ng/mL,P<0.001),while sCD22 levels were lower(14.3±2.1 ng/mL vs 18.9±3.4 ng/mL,P<0.01).Lp-PLA2 levels were also higher in the AD group(112.5±20.6 ng/mL vs 89.7±15.2 ng/mL,P<0.05).Significant differences were noted in CXCL12/sCD22(3.3 vs 1.7,P<0.001)and Lp-PLA-2/sCD22 ratios(8.0 vs 5.2,P<0.05)between the *** operating characteristic analysis confirmed high sensitivity and specificity of these markers and their ratios in distinguishing AD,with area under the curves ranging from CONCLUSION Serum CXCL12 and Lp-PLA2 levels were significantly increased,while sCD22 were significantly decreased,as well as increases in the ratios of CXCL12/sCD22 and Lp-PLA2/sCD22,are closely related to the onset of *** biomarkers and their ratios can be used as potential diagnostic indicators for AD,providing an important clinical reference for early intervention and treatment.

关键词： Alzheimer's disease Biomarkers CXCL12 sCD22 Lp-PLA2

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Correlative factors of poor prognosis and abnormal cellular immune function in patients with Alzheimer’s disease

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World Journal of Clinical Cases 2024年第6期12卷 1063-1075页

作者： Hua Bai Hong-Mei Zeng Qi-fang Zhang Yue-Zhi Hu fei-fei Deng Department of Neurology The Third Affiliated Hospital of Guizhou Medical University in ChinaDuyun 558099Guizhou ProvinceChina Department of Neurology Guizhou Medical UniversityDuyun 558099Guizhou ProvinceChina Key Laboratory of Medical Molecular Biology Guizhou Medical UniversityGuiyang 550004Guizhou ProvinceChina

BACKGROUND Alzheimer’s disease(AD)is a serious disease causing human dementia and social *** quality of life and prognosis of AD patients have attracted much *** role of chronic immune inflammation in the pathogenesis of AD is becoming more and more *** To study the relationship among cognitive dysfunction,abnormal cellular immune function,neuroimaging results and poor prognostic factors in *** A retrospective analysis of 62 hospitalized patients clinical diagnosed with AD who were admitted to our hospital from November 2015 to November *** cognitive dysfunction performance characteristics,laboratory test data and neuroimaging data from medical records within 24 h of admission,including Mini Mental State Examination Scale score,drawing clock test,blood T lymphocyte subsets,and neutrophils and lymphocyte ratio(NLR),disturbance of consciousness,extrapyramidal symptoms,electroencephalogram(EEG)and head nucleus magnetic spectroscopy(MRS)and other *** logistic regression analysis was used to determine indepen.ent prog-nostic *** modified Rankin scale(mRS)was used to determine whether the prognosis was *** correlation between drug treatment and prognostic mRS score was tested by the rank sum *** Univariate analysis showed that abnormal cellular immune function,extrapyramidal symptoms,obvious disturbance of consciousness,abnormal EEG,increased NLR,abnormal MRS,and complicated pneumonia were related to the poor prognosis of AD *** logistic regression analysis showed that the decrease in the proportion of T lym-phocytes in the blood after abnormal cellular immune function(odd ratio:2.078,95%confidence interval:1.156-3.986,Ppen.ent risk factor for predicting the poor prognosis of *** number of days of donepezil treatment to improve cognitive function was negatively correlated with mRS score(r=0.578,P<0.05).CONCLUSION The decrease in the proportion of T

关键词： Alzheimer’s disease Cellular immunity Prognosis T lymphocytes Magnetic resonance spectroscopy

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Association between serum estradiol level and appen.icular lean mass index in middle-aged postmenopausal women

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World Journal of Orthopedics 2024年第1期15卷 45-51页

作者： fang Jin Yan-fei Wang Zhong-Xin Zhu Department of Osteoporosis Care and Control Xiaoshan Affiliated Hospital of Wenzhou Medical UniversityHangzhou 311200Zhejiang ProvinceChina

BACKGROUND Previous studies investigating the association between loss of estrogen at menopause and skeletal muscle mass came to contradictory *** To evaluate the association between serum estradiol level and appen.icular lean mass index in middle-aged postmenopausal women using population-based *** This study included 673 postmenopausal women,aged 40-59 years,from the National Health and Nutrition Examination Survey between 2013 and *** multivariable linear regression models were used to evaluate the association between serum E2 Level and appen.icular lean mass index(ALMI).When non-linear associations were found by using weighted generalized additive model and smooth curve fitting,two-piecewise linear regression models were further applied to examine the threshold *** There was a positive association between serum E2 level and *** to individuals in quartile 1 group,those in other quartiles had higher ALMI *** inverted U-shaped curve relationship between serum E2 Level and ALMI was found on performing weighted generalized additive model and smooth curve fitting,and the inflection point was identified as a serum E2 level of 85 pg/*** Our results demonstrated an inverted U-shaped curve relationship between serum E2 levels and ALMI in middle-aged postmenopausal women,suggesting that low serum E2 levels play an important in the loss of muscle mass in middleaged postmenopausal women.

关键词： Estradiol Skeletal muscle Menopause Health The National Health and Nutrition Examination Survey

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A Sustainable Strategy for Spen. Cathode Carbon Blocks Hazardous Waste Recycling Using Binary Molten Salt Thermal Treatment

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Journal of Thermal Science 2024年第3期33卷 1082-1093页

作者： LI Bin DING Yan JIAO Yuyong XU fang WANG Xinyang ZHENG fei ZOU Junpen. GAO Qiang HU Hongyun Faculty of Engineering China University of GeosciencesWuhan430074China Institute for Natural Disaster Risk Prevention and Emergency Management China University of GeosciencesWuhan430074China State Key Laboratory of Coal Combustion Huazhong University of Science and TechnologyWuhan430074China

Waste-to-Energy treatment is a promising path to environment and energy management in the *** work detailed a binary molten salt thermal treatment methodology for the detoxification of spen. cathode carbon block(SCCB)waste and the recycling of carbonaceous *** thermal behavior of SCCB and SCCB blended with molten salts was *** was found that the NaCl-Na_(2)CO_(3)binary molten salts significantly contributed to reducing pyrolysis onset temperature by 334.3 K compared to that of SCCB itself(i.e.,activation energy of pyrolysis reaction was reduced from 4.24×10^(5)to 2.30×10^(5)J/mol),thus helping to lower thermal treatment energy *** the addition of binary molten salts,the residue after thermal treatment in a horizontal tube furnace experiment was separated into two *** bottom-layer residue was mainly composed of molten *** fluorine content in the form of NaF and CaF_(2)of top-layer residue was reduced significantly while the carbon content remained ***,the leaching concentration of fluoride ion was decreased from 4620 mg/L to 856 mg/*** is noted that the NaF and CaF_(2)can be removed through water-leaching and hydrothermal acid-leaching methods and thus the carbonaceous materials with a calorific value of 17.5 MJ/kg were obtained.

关键词： spen. cathode carbon block hazardous solid waste detoxification binary-molten-salt thermal treatment carbon recycling pyrolysis kinetics

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