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The neuroprotection of siRT1 on NMDA-induce excitotoxicity in cultured cortical cell

中国神经科学学会第十届全国学术会议

作者： Xue-Fei SUN pei-pei si Yu ZHANG Xiao-Rong YANG Ce ZHANG Department of Physiology Shanxi Medical University

Objective The silent information regulator the factor 2 enzyme 1(silent information regulator 2 homolog 1,siRT1) is one of the members of the sirtuin family,widely presents in mammalian *** recent years,people have paid more and more attention on the neuroprotective effect of *** researches have suggested that many factors can regulate siRT1 ***,specific intracellular signaling mechanism does not *** The study was divided into three *** NMDA-induced Excitotoxicity cell model,apphed to Gene silencing technology,Genetic mutations,Western blotting(WB),Real Time PCR,immunoprecipitation(IP),the enzyme activity detection experiments and so on to observe(1) the effects of HuR phosphorylation to siRT1 in NMDA-induced Excitotoxicity,(2) the effects of PKC kinase activity to HuR in NMDA-induced Excitotoxicity,(3) the effects of influx of Ca to PKC kinase activity in NMDA-induced *** In NMDA-induced excitotoxicity cell model,NMDA induces extracellular Ca influx,intracellular increased Ca activates PKC,activation of PKC phosphorylates HuR,the phosphorylated HuR with siRT1 mRNA combines reduces,so that siRT1 mRNA decreases stability,siTR1downregulates,causing cell damages and even to *** Ca-PKC-HuR-siRTl pathway may be the access to regulating siRT1 function in neurons.

关键词： NMDA excitatory neurotoxicity siRT1 neuroprotection signaling pathways

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The neuroprotection of SIRT1 on NMDA-induce excitotoxicity i...

A new remaining useful life estimation method for equipment subjected to intervention of imperfect maintenance activities

中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Hua-Ping QIN,Rui WANG,pei-pei si,Xiao-Rong YANG~*,Ce ZHANG~* Department of Physiology National Key Disciplines,Key Laboratory for Cellular Physiology of Ministry of Education,Shanxi Medical University,Taiyuan 030001,China

Objective silent information regulator 1(siRTl),an NAD-dependent deacetylase,is involved in the regulation of gene transcription,energy metabolism,and cell *** studies have showed that siRTl possesses neuroprotective effects,however,it is unknown whether it protects neurons from NMDA-mediated neurotoxicity. Here we observed the role of siRTl in the cellular model of NMDA-mediated neurotoxicity(100 umol/L,2 h) using resveratrol as siRTl *** Cell viability was measured by WST-8 assay,LDH levels assay and Calcein-AM *** levels of siRTl protein,P53 protein,and acetylation of P53 were identified by Westem-blot. siRTl mRNA levels was detected by ***(1) Treatment of cultured cortical cell with resveratrol elicited dose-dependent inhibitory effects on NMDA-induced ***(25μmol/L) pre-incubated for 12 h inhibited NMDA-induced decrease of cell viability by 26.07%(Psis showed that resvertrol could not inhibit NMDA-induced decrease of siRTl protein and mRNA levels,however,resvertrol could suppress NMDA-induced increase of the acetylation of P53,a downstream target of siRTl activation,which means under excitotoxic conditions,resvertrol increased activity of siRTl to protect neurons,in part by hindering the acetylation of *** The present study demonstrated the neuroprotective roles of siRTl in NMDA-induced excitotoxicity in cortical neurons,therefore,siRTl could be a novel target for preventing and treating neurodegeneration.

关键词： silent information regulator 1 neuroprotection NMDA excitotoxicity

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Chinese Journal of Aeronautics 2018年第3期31卷 514-528页

作者： Changhua HU Hong pei Zhaoqiang WANG Xiaosheng si Zhengxin ZHANG College of Science High-Tech Institute of Xi'an

As the key part of Prognostics and Health Management（PHM）, Remaining Useful Life（RUL） estimation has been extensively investigated in recent years. Current RUL estimation studies considering the intervention of imperfect maintenance activities usually assumed that maintenance activities have a single influence on the degradation level or degradation rate, but not on *** at this problem, this paper proposes a new degradation modeling and RUL estimation method taking the influence of imperfect maintenance activities on both the degradation level and the degradation rate into account. Toward this end, a stochastic degradation model considering imperfect maintenance activities is firstly constructed based on the diffusion process. Then, the Probability Density Function（PDF） of the RUL is derived by the convolution operator under the concept of First Hitting Time（FHT）. To implement the proposed RUL estimation method,the Maximum Likelihood Estimation（MLE） is utilized to estimate the degradation related parameters based on the Condition Monitoring（CM） data, while the Bayesian method is utilized to estimate the maintenance related parameters based on the maintenance data. Finally, a numerical example and a practical case study are provided to demonstrate the superiority of the proposed method. The experimental results show that the proposed method could greatly improve the RUL estimation accuracy for the degrading equipment subjected to imperfect maintenance activities.

关键词： Convolution operator Diffusion process First hitting time Imperfect maintenance Remaining useful life

Numerical and Experimental Study on Flow-induced Noise at Blade-passing Frequency in Centrifugal Pumps

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Chinese Journal of Mechanical Engineering 2014年第3期27卷 606-614页

作者： YANG Jun YUAN Shouqi YUAN Jianping si Qiaorui pei Ji Research Center of Fluid Machinery Engineering and Technology Jiangsu University

With the increasing noise pollution, low noise optimization of centrifugal pimps has become a hot topic. However, experimental study on this problem is unacceptable for industrial applications due to unsustainable cost. A hybrid method that couples computational fluid dynamics （CFD） with computational aeroacoustic software is used to predict the flow-induced noise of pumps in order to minimize the noise of centrifugal pumps in actual projects. Under Langthjem＇s assumption that the blade surface pressure is the main flow-induced acoustic source in centrifugal pumps, the blade surface pressure pulsation is considered in terms of the acoustical sources and simulated using CFX software. The pressure pulsation and noise distribution in the near-cutoff region are examined for the blade-passing frequency （BPF） noise, and the sound pressure level （SPL） reached peaks near the cutoff that corresponded with the pressure pulsation in this region. An experiment is performed to validate this prediction. Four hydrophones are fixed to the inlet and outlet ports of the test pump to measure the flow-induced noise from the four-port model. The simulation results for the noise are analyzed and compared with the experimental results. The variation in the calculated noise with changes in the flow agreed well with the experimental results. When the flow rate was increased, the SPL first decreased and reached the minimum near the best efficient point （BEP）; it then increased when the flow rate was further increased. The numerical and experimental results confirmed that the BPF noise generated by a blade-rotating dipole roughly reflects the acoustic features of centrifugal pumps. The noise simulation method in current study has a good feasibility and suitability, which could be adopted in engineering design to predict and optimize the hydroacoustic behavior of centrifugal pumps.

关键词： centrifugal pumps flow-induced noise prediction blade-passing noise blade surface pressure pulsations

Chromatic Sums of Biloopless Nonseparable Near-Triangulations on the Projective Plane

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Acta Mathematicae Applicatae sinica 2013年第1期29卷 123-134页

作者： Zhao-xiang Li Yan-pei Liu Bing-feng si Department of Mathematics Minzu University of China Department of Mathematics Beijing Jiaotong University School of Traffic and Transportation Beijing Jiaotong University

In this paper, the chromatic sum functions of rooted biloopless nonsepavable near-triangulations on the sphere and the projective plane are studied. The chromatic sum function equations of such maps are obtained. From the chromatic sum equations of such maps, the enumerating function equations of such maps are derived. An asymptotic evaluation and some explicit expression of enumerating functions are also derived.

关键词： chromatic sum function biloopless triangulation enumerating function

Down-regulation of Risa improves podocyte injury by enhancing autophagy in diabetic nephropathy

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Military Medical Research 2023年第1期10卷 32-44页

作者： pei-pei Su Dong-Wei Liu si-Jie Zhou Hang Chen Xian-Ming Wu Zhang-Suo Liu Department of Nephrology the First Affiliated Hospital of Zhengzhou UniversityResearch Institutes of NephropathyZhengzhou UniversityZhengzhou 450052China Department of Nephrology and Rheumatology the Third People’s Hospital of ZhengzhouZhengzhou 450002China Henan Province Research Center for Kidney Disease Zhengzhou 450052China Key Laboratory of Precision Diagnosis and Treatment for Chronic Kidney Disease in Henan Province Zhengzhou 450002China

Background: LncRNA AK044604(regulator of insulin sensitivity and autophagy, Risa) and autophagy-related factors sirt1 and GSK3β play important roles in diabetic nephropathy(DN). In this study, we sought to explore the effect of Risa on sirt1/GSK3β-induced podocyte ***: Diabetic db/db mice received Risa-inhibition adeno-associated virus(AAV) via tail vein injection, and intraperitoneal injection of lithium chloride(LiCl). Blood, urine, and kidney tissue samples were collected and analyzed at different time points. Immortalized mouse podocyte cells(MPCs) were cultured and treated with Risa-inhibition lentivirus(LV), EX-527, and LiCl. MPCs were collected under different stimulations as noted. The effects of Risa on podocyte autophagy were examined by qRT-PCR, Western blotting analysis, transmission electron microscopy,Periodic Acid-Schiff staining, and immunofluorescence ***: Risa and activated GSK3β were overexpressed, but sirt1 was downregulated in DN mice and high glucosetreated MPCs(Psis. Risa overexpression attenuated sirt1-mediated downstream autophagy levels and aggravated podocyte injury by inhibiting the expression of sirt1(Psion enhanced sirt1-induced autophagy and attenuated podocyte injury, which could be abrogated by EX-527(Psion aggravated podocyte injury by decreasing ***: Risa could inhibit autophagy by regulating the sirt1/GSK3β axis, thereby aggravating podocyte injury in DN. Risa may serve as a therapeutic target for the treatment of DN.

关键词： Diabetic nephropathy LncRNA AK044604/sirt1/GSK3β Autophagy

Oral absorption of hyaluronic acid and phospholipids complexes in rats

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World Journal of Gastroenterology 2007年第6期13卷 945-949页

作者： si-Ling Huang pei-Xue Ling Tian-Min Zhang Working Station for Postdoctoral Scientific Research Institute of Biopharmaceuticals of Shandong Province Jinan 250108 Shandong Province China

AIM： To prepare a complex of hyaluronic acid （HA） and phospholipids （PL）, and study the improvement effect of PL on the oral absorption of HA. METHODS： The complex of HA-PL （named Haplex） was prepared by film dispersion and sonication method, its physico-chemical properties were identified by infrared spectra and differential scanning calorimetry （DSC）. The oral absorption of Haplex was studied. Thirty-two healthy rats were divided into 4 groups randomly：（1） a normal saline （NS） control group; （2） an HA group; （3） a mixture group and （4） a Haplex group. After intragastric administration, the concentration of HA in serum was determined. RESULTS： The physico-chemical properties of Haplex were different from HA or PL or their mixture. After Haplex was administered to rats orally, the serum concentration of HA was increased when compared with the mixture or HA control groups from 4 h to 10 h （P 〈 0.05）. The AAUCo-12, of Haplex was also greater than that of the other three groups （P 〈 0.05）. CONCLUsiON： The method of film dispersion and sonication can prepare HA and PL complex, and PL can enhance the oral absorption of exogenous HA.

关键词： Hyaluronic acid Phospholipids Complex Oral absorption Infrared spectrum Thermochemistry

Low-dimensional non-metal catalysts:principles for regulating p-orbital-dominated reactivity

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npj Computational Materials 2021年第1期7卷 1699-1732页

作者： si Zhou Wei pei Yanyan Zhao Xiaowei Yang Nanshu Liu Jijun Zhao Key Laboratory of Materials Modification by Laser Ion and Electron Beams(Dalian University of Technology)Ministry of EducationDalian 116024China

Activation of p-block elements to replace the rare and precious transition metals for renewable energy applications is highly *** this review,we go over recent experimental and theoretical progress on the low-dimensional non-metal materials for clean energy production,including carbon,silicon,oxide,boron,and phosphorus-based nanostructures,with the p-block elements serving as active *** aim to elucidate the mechanism for triggering activity in different kinds of non-metal systems,and extract general principles for controlling the p-orbital-mediated reactivity from a theoretical point of *** perspectives and challenges for developing high-efficiency non-metal catalysts are provided in the end.

关键词： reactivity orbital principles

Integrating multisource RS data and GIS techniques to assist the evaluation of resource-environment carrying capacity in karst mountainous area

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Journal of Mountain Science 2020年第10期17卷 2528-2547页

作者： PU Jun-wei ZHAO Xiao-qing MIAO pei-pei LI si-nan TAN Kun WANG Qian TANG Wei School of Earth Sciences Yunnan UniversityKunming650500YunnanChina Institute of International Rivers&Eco-security Yunnan UniversityKunming650500YunnanChina

The karst mountainous area is an ecologically fragile region with prominent humanland *** resource-environment carrying capacity(RECC)of this region needs to be further *** development of remote sensing(RS)and geographic information system(GIS)provides data sources and processing platform for RECC *** study analyzed and established the evaluation index system of RECC by considering particularity in the karst mountainous area of Southwest China;processed multisource RS data(Sentinel-2,Aster-DEM and Landsat-8)to extract the spatial distributions of nine key indexes by GIS techniques(information classification,overlay analysis and raster calculation);proposed the methods of index integration and fuzzy comprehensive evaluation of the RECC by GIS;and took a typical area,Guangnan County in Yunnan Province of China,as an experimental area to explore the effectiveness of the indexes and *** results showed that:(1)The important indexes affecting the RECC of karst mountainous area are water resources,tourism resources,position resources,geographical environment and soil erosion environment.(2)Data on cultivated land,construction land,minerals,transportation,water conservancy,ecosystem services,topography,soil erosion and rocky desertification can be obtained from RS *** techniques integrate the information into the RECC *** data extraction and processing methods are feasible on evaluating RECC.(3)The RECC of Guangnan County was in the mid-carrying level in *** midcarrying and low-carrying levels were the main types,accounting for more than 80.00%of the total study *** areas with high carrying capacity were mainly distributed in the northern regions of the northwest-southeast line of the county,and other areas have a low carrying capacity *** coordination between regional resource-environment status and socioeconomic development is the key to improve *** study explores the evaluation index

关键词： Carrying capacity Multisource RS data GIS techniques Evaluation index system Data Integration Karst mountainous area Fuzzy comprehensive evaluation method