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液相沉淀法制备高纯超细阻燃型氢氧化镁

塑料科技 2024年第2期52卷 94-99页

作者：曾书航杜莹陈婷李智力裴丰郭忠何东升唐远杨晶晶武汉工程大学资源与安全工程学院湖北武汉430073 湖北宜化化工科技研发有限公司湖北宜昌443300

以硫酸镁为原料,氢氧化钠为沉淀剂,加入适量硬脂酸钠(NaSt)和聚乙二醇(PEG2000)作为表面活性剂,采用液相沉淀法制备高纯超细阻燃型氢氧化镁粉体。考察沉淀方式、反应物配比、反应温度、搅拌速率、反应时间、表面活性剂配比、表面活性剂... 详细信息

以硫酸镁为原料,氢氧化钠为沉淀剂,加入适量硬脂酸钠(NaSt)和聚乙二醇(PEG2000)作为表面活性剂,采用液相沉淀法制备高纯超细阻燃型氢氧化镁粉体。考察沉淀方式、反应物配比、反应温度、搅拌速率、反应时间、表面活性剂配比、表面活性剂用量和洗涤方式等对氢氧化镁粉体粒径的影响。测试了产物形貌、晶相结构、粒径及粒径分布、纯度、分散效果和润湿性。结果表明:制备超细氢氧化镁的适宜的工艺条件包括,沉淀方式为双向沉淀,n(NaOH)∶n(MgSO4)=2.5∶1.0,反应温度为60℃,搅拌速率为400 r/min,反应时间为50 min,NaSt/PEG2000的配比为4∶1、添加量为4 g/mol MgSO4,洗涤方式为纯水洗涤3次。所得的氢氧化镁表面呈疏水性,接触角达123.50°,分散性好,纯度高达99.50%,平均粒径为3.83μm,粒度分布均匀并呈六方片状晶体。样品白度、纯度和粒径满足阻燃剂用氢氧化镁指标。

关键词：超细氢氧化镁表面改性表面活性剂阻燃剂

CRISPR/Cas9 therapeutics:progress and prospects

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Signal Transduction and Targeted Therapy 2023年第2期8卷 558-580页

作者： Tianxiang Li Yanyan Yang Hongzhao Qi Weigang Cui Lin Zhang Xiuxiu Fu Xiangqin He Meixin Liu pei-feng Li Tao Yu Institute for Translational Medicine The Affiliated Hospital of Qingdao UniversityNo.38 Dengzhou Road266021QingdaoPeople’s Republic of China Department of Immunology School of Basic MedicineQingdao University266021QingdaoPeople’s Republic of China Department of Cardiology People’s Hospital of RizhaoNo.126 Taian Road276827RizhaoPeople’s Republic of China Department of Microbiology Linyi Center for Disease Control and Prevention276000LinyiPeople’s Republic of China Department of Cardiac Ultrasound The Affiliated Hospital of Qingdao University266000QingdaoPeople’s Republic of China

Clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)gene-editing technology is the ideal tool of the future for treating diseases by permanently correcting deleterious base mutations or disrupting disease-causing genes with great precision and efficiency.A variety of efficient Cas9 variants and derivatives have been developed to cope with the complex genomic changes that occur during ***,strategies to effectively deliver the CRISPR system to diseased cells in vivo are currently lacking,and nonviral vectors with target recognition functions may be the focus of future *** and physiological changes resulting from disease onset are expected to serve as identifying factors for targeted delivery or targets for gene *** are both varied and complex,and the choice of appropriate gene-editing methods and delivery vectors for different diseases is ***,there are still many potential challenges identified when targeting delivery of CRISPR/Cas9 technology for disease *** paper reviews the current developments in three aspects,namely,gene-editing type,delivery vector,and disease ***,this paper summarizes successful examples of clinical trials and finally describes possible problems associated with current CRISPR applications.

关键词： CRISPR/Cas9 editing delivery

西方蜜蜂AmAGO1蛋白的分子特性、时空表达谱及抗体制备

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昆虫学报 2024年第1期67卷 18-28页

作者：叶亚萍刘治滩李琪明臧贺冯佩林王宁王杰黄枳腱陈大福郭睿福建农林大学蜂学与生物医药学院福州350002 天然生物毒素国家地方联合工程实验室福州350002 福建省蜂疗研究所福州350002 四川省农业农村厅畜牧总站成都610041

【目的】Argonaute(AGO)家族是一类在进化上高度保守的蛋白家族,其在真核生物中主要参与形成RNA诱导沉默复合体(RNA-induced silencing complex,RISC),进而通过沉默基因表达参与诸多生物学过程。蜜蜂AGO蛋白的相关研究迄今仍然缺失。本... 详细信息

【目的】Argonaute(AGO)家族是一类在进化上高度保守的蛋白家族,其在真核生物中主要参与形成RNA诱导沉默复合体(RNA-induced silencing complex,RISC),进而通过沉默基因表达参与诸多生物学过程。蜜蜂AGO蛋白的相关研究迄今仍然缺失。本研究拟通过预测西方蜜蜂Apis mellifera的AGO1(AmAGO1)理化性质和分子特性,解析AmAGO1在西方蜜蜂中的时空表达谱,并制备AmAGO1的多克隆抗体,为深入开展AmAGO1的功能与机制研究提供参考和基础。【方法】PCR扩增西方蜜蜂AmAGO1的编码序列(coding sequence,CDS);通过生物信息学预测AmAGO1蛋白的理化性质和分子特性;利用RT-qPCR检测AmAGO1在西方蜜蜂工蜂卵、3日龄幼虫、7日龄预蛹、8日龄预蛹、12日龄蛹以及1,2,6,12,15和18日龄成虫以及工蜂成虫触角、咽下腺、脑、表皮、中肠、脂肪体和毒腺中的表达量;构建原核表达质粒后诱导表达AmAGO1融合蛋白,并鉴定其表达形式;制备AmAGO1多克隆抗体,利用ELISA,Western blot和免疫沉淀(immunoprecipitation,IP)分别检测抗体效价、灵敏度和特异性。【结果】成功克隆到西方蜜蜂AmAGO1的CDS;AmAGO1含928个氨基酸,分子式为C4624H7332N1316O1325S51,分子量约为104.2 kD,等电点为9.31,平均亲水系数为-0.2965,含86个磷酸化位点及典型的PAZ结构域和PIWI结构域,但不存在典型的信号肽;AGO1在智人Homo sapiens、斑马鱼Danio rerio、黑腹果蝇Drosophila melanogaster、家蚕Bombyx mori、西方蜜蜂、东方蜜蜂***和欧洲熊蜂Bombus terrestris中具有较高的氨基酸序列一致性;西方蜜蜂和东方蜜蜂的AGO1聚为一支,同源性最高。AmAGO1在西方蜜蜂工蜂卵、幼虫、预蛹、蛹和成虫中均有表达但表达量存在差异,3日龄幼虫和7日龄预蛹中AmAGO1的表达量显著低于卵中的表达量,而8日龄预蛹和12日龄蛹中AmAGO1的表达量显著高于卵中的表达量;AmAGO1在1,2,6,12,15和18日龄工蜂体内均有表达但表达量也存在差异,其中1日龄成虫体内AmAGO1的表达量显著高于其他日龄成虫体内的表达量;AmAGO1在工蜂成虫触角、毒腺、脑、中肠、咽下腺、脂肪体和表皮中均有表达但表达量存在差异,触角中AmAGO1的表达量与咽下腺中的相差无几,但二者均显著高于毒腺、脑、中肠、脂肪体和表皮中AmAGO1的表达量;AmAGO1融合蛋白的表达形式为包涵体;制备的AmAGO1多克隆抗体具有较高的效价、灵敏度和特异性。【结论】AmAGO1可能是亲水性胞内蛋白,含有典型的PAZ结构域和PIWI结构域;AmAGO1在西方蜜蜂工蜂不同组织和不同发育阶段发挥潜在的重要功能;成功制备出高效价、高灵敏度和强特异性的AGO1多克隆抗体。

关键词：西方蜜蜂 AmAGO1 克隆分子特性时空表达谱原核表达多克隆抗体

Three-Layer Joint Modeling of Chinese Trigger Extraction withConstraints on Trigger and Argument Semantics

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Journal of Computer Science & Technology 2017年第5期32卷 1044-1056页

作者： pei-feng Li Guo-Dong Zhou School of Computer Science and Technology Soochow University Suzhou 215006 China

As a subtask of information extraction (IE), which aims to extract structured information from texts, event extraction is to recognize event trigger mentions of a predefined event type and their arguments. In general, event extraction can be divided into two subtasks: trigger extraction and argument extraction. Currently, the frequent existences of unannotated trigger mentions and poor-context trigger mentions impose critical challenges in Chinese trigger extraction. This paper proposes a novel three-layer joint model to integrate three components in trigger extraction, i.e., trigger identification, event type determination, and event subtype determination. In this way, different kinds of evidence on distinct pseudo samples can be well captured to eliminate the harmful effects of those un-annotated trigger mentions. In addition, this paper introduces various types of linguistically driven constraints on the trigger and argument semantics into the joint model to recover those poor-context trigger mentions. The experimental results show that our joint model significantly outperforms the state-of-the-art Chinese trigger extraction and Chinese event extraction as a whole.

关键词： information extraction trigger extraction joint modeling trigger semantics argument semantics

An Active Deception Defense Model Based on Address Mutation and Fingerprint Camouflage

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China Communications 2024年第7期21卷 212-223页

作者： Wang Shuo Chu Jiang pei Qingqi Shao feng Yuan Shuai Zhong Xiaoge State Key Laboratory of Astronautic Dynamics Xi’an Satellite Control CenterXi’an 710043China State Key Laboratory of Integrated Services Networks Xidian UniversityXi’an 710071China Shaanxi Key Laboratory of Blockchain and Secure Computing Xidian UniversityXi’an 710071China

The static and predictable characteristics of cyber systems give attackers an asymmetric advantage in gathering useful information and launching *** reverse this asymmetric advantage,a new defense idea,called Moving Target Defense(MTD),has been proposed to provide additional selectable measures to complement traditional ***,MTD is unable to defeat the sophisticated attacker with fingerprint tracking *** overcome this limitation,we go one step beyond and show that the combination of MTD and Deception-based Cyber Defense(DCD)can achieve higher performance than either of *** particular,we first introduce and formalize a novel attacker model named Scan and Foothold Attack(SFA)based on cyber kill ***,we develop probabilistic models for SFA defenses to provide a deeper analysis of the theoretical effect under different defense *** models quantify attack success probability and the probability that the attacker will be deceived under various conditions,such as the size of address space,and the number of hosts,attack analysis ***,the experimental results show that the actual defense effect of each strategy almost perfectly follows its probabilistic ***,the defense strategy of combining address mutation and fingerprint camouflage can achieve a better defense effect than the single address mutation.

关键词： address mutation deception defense fingerprint camouflage moving target defense probabilistic model

Efficient and stable PtFe alloy catalyst for electrocatalytic methanol oxidation with high resistance to CO

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Journal of Energy Chemistry 2024年第3期90卷 327-336,I0008页

作者： Qian Yang Sifan Zhang fengshun Wu Lihua Zhu Guang Li Mingzhi Chen An pei Yingliang feng Jiangxi Provincial Key Laboratory of Functional Molecular Materials Chemistry College of Chemistry and Chemical EngineeringFaculty of Materials Metallurgy and ChemistryJiangxi University of Science and TechnologyGanzhou 341000JiangxiChina College of Chemistry and Chemical Engineering Xiamen UniversityXiamen 361005FujianChina

Direct methanol fuel cells(DMFC) are widely considered to be an ideal green energy conversion device but their widespread applications are limited by the high price of the Pt-based catalysts and the instability in terms of surface CO toxicity in long-term ***,the PtFe alloy nanoparticles(NPs) with small particle size(~4.12 nm) supported on carbon black catalysts with different Pt/Fe atomic ratios(Pt_(1)Fe_(2)/C,Pt_(3)Fe_(4)/C,Pt_(1)Fe_(1)/C,and Pt_(2)Fe_(1)/C) are successfully prepared for enhanced anti-CO poisoning during methanol oxidation reaction(MOR).The optimal atomic ratio of Pt/Fe for the MOR is 1:2,and the mass activity of Pt_(1)Fe_(2)/C(5.40 A mg_(Pt)^(-1)) is 13.5 times higher than that of conventional commercial Pt/C(Pt/C-JM)(0.40 A mg_(Pt)^(-1)).The introduction of Fe into the Pt lattice forms the PtFe alloy phase,and the electron density of Pt is reduced after forming the PtFe ***-situ Fourier transform infrared results indicate that the addition of oxyphilic metal Fe has reduced the adsorption of reactant molecules on Pt during the *** doping of Fe atoms helps to desorb toxic intermediates and regenerate Pt active sites,promoting the cleavage of C-O bonds with good selectivity of CO_(2)(58.1%).Moreover,the Pt_(1)Fe_(2)/C catalyst exhibits higher CO tolerance,methanol electrooxidation activity,and long-term stability than other Pt_(x)Fe_(y)/C catalysts.

关键词： Alloy catalyst PtFe Methanol oxidation In-situ FTIR CO resistance

Hortaea werneckii infections:what else besides tinea nigra?

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Hortaea werneckii infections:what else besides tinea nigra?

中国菌物学会2023年学术年会

作者： pei-ying feng Department of Dermatology Third Affiliated HospitalSun Yat-sen University

Tinea nigra is a superficial phaeohyphomycosis caused by the black yeast Hortaea *** disorder is characterized by asymptomatic pigmented macules on the palms or *** may be confused with other types of pigmentary lesions,and it can be misdiagnosed because of its rarity and variable *** describe four cases of *** infections,including two typical tinea nigra,as well as two atypical cases,*** eczema-like lesion of the hand,and a finger nail involvement,*** was confirmed by phenotypic and molecular data,as well as histopathology and *** cases expand the clinical spectrum of *** infections.

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五运六气理论与《临证指南医案》中风证治

世界中医药 2024年第6期19卷 818-821,825页

作者：张玉鑫刘晓燕崔亚东张文静梁羽茜裴枫邰雪莉黄雪杰北京中医药大学北京100029

五运六气学说是《黄帝内经》天人合一思想的重要组成部分,也是中医思维的一大特色,更是中医科学性的重要体现。叶桂承经旨而博采众长,在《临证指南医案》中记载了诊疗中风的宝贵经验,其中蕴含着五运六气理论的应用经验,叶氏认为,风木司... 详细信息

五运六气学说是《黄帝内经》天人合一思想的重要组成部分,也是中医思维的一大特色,更是中医科学性的重要体现。叶桂承经旨而博采众长,在《临证指南医案》中记载了诊疗中风的宝贵经验,其中蕴含着五运六气理论的应用经验,叶氏认为,风木司天容易出现气火上亢,脾土受克又兼气机上下滞塞,而天符岁会年气候变化剧烈,阳气开泄,阴不内守而中风。中风发病特点与时令之气有关,厥阴风木主时与火热之气当令,耗气伤津,气津输布异常致络脉空虚而中风;冬令天反暖失于封藏,多肾阴暗耗而动风。针对上述病机,其中厥阴风木当令,脾胃受邪,故治以培补中气,同时兼以敛降之品调节人体升而不降的气机状态;火热当令,阳气发泄,致络脉空虚而中风者,补益脾胃以实卫气;冬令当寒反暖,致肾精不固而中风者,则以滋水涵木为法。叶氏重视外界环境因素对病机的影响,将天人相应的思想运用于中风的辨证论治中,示人以法,值得学习和借鉴。

关键词：五运六气《临证指南医案》中风证治规律叶桂天人相应时令病机

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从标本中气理论探讨“肝生于左,肺藏于右”的内涵

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世界中医药 2024年第4期19卷 529-532页

作者：崔亚东刘晓燕梁羽茜张玉鑫张文静裴枫邰雪莉黄雪杰北京中医药大学中医学院北京100029

“肝生于左,肺藏于右”是中医中重要的理论问题,自提出以来就有很多解释,而这些解释也有不尽相同的地方,本文从五运六气中的标本中气理论出发,对“肝生于左,肺藏于右”的理论进行分析,提出了“肝生于左,肺藏于右”是指“肝主阳、肺主阴... 详细信息

“肝生于左,肺藏于右”是中医中重要的理论问题,自提出以来就有很多解释,而这些解释也有不尽相同的地方,本文从五运六气中的标本中气理论出发,对“肝生于左,肺藏于右”的理论进行分析,提出了“肝生于左,肺藏于右”是指“肝主阳、肺主阴的功能”的观点,且其理论内涵对临床实践有较好的指导意义。

关键词：肝生于左肺藏于右五运六气标本中气肝主阳肺主阴