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Experimental salt cavern in offshore ultra-deep water and well designevaluation for CO_(2) abatement

International Journal of mining Science and Technology 2019年第5期29卷 641-656页

作者： Alvaro maia da costa pedro v.m.costa Antonio C.O.miranda mariana B.R.Goulart Okhiria D.Udebhulu Nelson F.F.Ebecken Ricardo C.Azevedo Sérgio m.de Eston Giorgio de Tomi Andre B.mendes Julio R.meneghini Kazuo Nishimoto Claudio mueller Sampaio Camila Brandao Alexandre Breda University of Sao Paulo(USP) Sao Paulo 01000-000Brazil modecom-Technology in Geomechanics and Computing modeling Rio de Janeiro 27570-000Brazil Department of Civil and Environment Engineering University of Brasília(UnB)Brasília 70000-000Brazil COPPE Federal University of Rio de JaneiroRio de Janeiro 27570-000Brazil Technology Division of Shell Shell BrazilRio de Janeiro 27570-000Brazil

This paper presents a proposal for an experimental salt cavern in offshore ultra-deep water for CO2 abatement,including the instrumentation plan and well conceptual design evaluated for carbon capture and storage(CCS)*** studies are based on applied computational mechanics associated with field experimentation that has contributed to the technical feasibility of the underground potash mine at the State of Sergipe in *** knowhow allowed the stability analysis of several salt caverns for brine production at the State of Alagoas in Brazil and to the drilling through stratified thick layers of salt of the pre-salt reservoirs in Santos ***,this knowledge has been applied in the design of onshore and offshore salt caverns opened by dissolution for storage of natural gas and *** geomechanical study,through the application of computational mechanics,of offshore giant salt caverns of 450 m high by 150 m in diameter,shows that one cavern can store about 4 billion Sm3 or 7.2 million tons of *** the construction of the giant cavern,which will be the first gas storage offshore in the world,it has been decided to develop an experimental one,with smaller size,to obtained field *** experimental cavern will allow the calibration of parameters to be used in the structural integrity analysis of the cavern and well for storage of natural gas which is rich in CO2 under high pressure.

关键词： Salt cavern Pre-salt reservoir Geomechanical study Carbon capture and storage

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Nanotubes in a Gradient Electric Field as Revealed by STm TEm Technique

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Nano Research 2008年第2期1卷 166-175页

作者： Dmitri Golberg pedro m.F.J.costa masanori mitome Yoshio Bando Nanoscale materials Center Namiki 1-1TsukubaIbaraki 305-0044Japan International Center for materials Nanoarchitectonics(mANA) National Institute for Materials ScienceNamiki 1-1TsukubaIbaraki 305-0044Japan Center for Research in Ceramics and Composite materials University of Aveiro3810-193 AveiroPortugal

We have investigated the behavior of two nanotube systems,carbon and boron nitride,under controlled applied voltages in a high-resolution transmission electron microscope(TEm)equipped with a scanning tunneling microscope(STm)*** nanotubes(or thin bundles)were positioned between a piezo-movable gold electrode and a biased(up to±140 v)STm tip inside the pole-piece of the *** structures studied include double-and multi-walled carbon nanotubes(the latter having diverse morphologies due to the various synthetic procedures utilized),few-layered boron nitride nanotube bundles and multi-walled boron nitride nanotubes(with or without functionalized surfaces).The electrical breakdown,physical failure,and electrostatic interactions are documented for each system.

关键词： Carbon nanotubes boron nitride nanotubes transmission electron microscope scanning tunneling microscope

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RNA Polymerase v Functions in Arabidopsis Interphase Heterochromatin Organization Independently of the 24-nt siRNA-Directed DNA methylation Pathway

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molecular Plant 2009年第4期2卷 700-710页

作者： Olga Pontes pedro costa-Nunes Paul vithayathil Craig S. Pikaard Biology Department Washington University 1 Brookings Drive St Louis MO 63130 USA

In Arabidopsis, pericentromeric repeats, retroelements, and silenced rRNA genes are assembled into heterochromatin within nuclear structures known as chromocenters. The mechanisms governing higher-order heterochromatin organization are poorly understood but 24-nt small interfering RNAs （siRNAs） are known to play key roles in heterochromatin formation. Nuclear RNA polymerase Iv （Pol Iv）, RNA-DEPENDENT RNA POLYmERASE 2 （RDR2）, and DICER-LIKE 3 （DCL3） are required for biogenesis of 24-nt siRNAs that associate with ARGONAUTE 4 （AGO4）. Nuclear RNA polymerase v （Pol v） collaborates with DRD1 （DEFICIENT IN RNA-DEPENDENT DNA mETHYLATION 1） to generate transcripts at heterochromatic loci that are hypothesized to bind to siRNA-AGO4 complexes and subsequently recruit the de-novo DNA methylation and/or histone modifying machinery. Here, we report that decondensation of the major pericentromeric repeats and depletion of the heterochromatic mark histone H3 lysine 9 dimethylation at chromocenters occurs specifically in pol v and drdl mutants. Disruption of pericentromeric repeats condensation is coincident with transcriptional reactivation of specific classes of pericentromeric 180-bp repeats. We further demonstrate that Pol v functions independently of Pol Iv, RDR2, and DCL3-mediated siRNA production to affect interphase heterochromatin organization, possibly by involving RNAs that recruit structural or chromatin-modifying proteins.

关键词： RNA polymerase v RNA-directed DNA methylation heterochromatin centromere gene expression.

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P2X7 receptor blockade decreases inflammation,apoptosis,and enteric neuron loss during Clostridioides difficile toxin A-induced ileitis in mice

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World Journal of Gastroenterology 2022年第30期28卷 4075-4088页

作者： Ana A Q A Santos Deiziane v S costa Danielle A Foschetti Antoniella S G Duarte Conceicao S martins pedro m G Soares Patricia Castelucci Gerly A C Brito Department of morphology School of MedicineFederal University of CearaFortaleza 60430-170CearaBrazil Department of Physiology and Pharmacology School of MedicineFederal University of CearáFortaleza 60430-170CearaBrazil Department of Pathology and Legal medicine School of MedicineFederal University of CearaFortaleza 60430-170CearaBrazil Department of morphology(UFC) Federal University of CearaFortaleza 60430-170CearaBrazil Department of Anatomy Institute of Biomedical SciencesUniversity of São PauloSao Paulo 05508-270Brazil Department of morphology Federal University of CearaFortaleza 60140-170CearaBrazil

Clostridioides difficile(***)is the most common pathogen causing health care-associated *** TcdA and TcdB have been shown to activate enteric neurons;however,what population of these cells is more profoundly influenced and the mechanism underlying these effects remain *** To characterize a specific population of TcdA-affected myenteric neurons and investigate the role of the P2X7 receptor in TcdA-induced ileal inflammation,cell death,and the changes in the enteric nervous system in *** Swiss mice were used to model TcdA-induced ileitis in ileal loops exposed to TcdA(50μg/Loop)for 4 *** investigate the role of the P2X7 receptor,Brilliant Blue G(50 mg/kg,i.p.),which is a nonspecific P2X7 receptor antagonist,or A438079(0.7μg/mouse,i.p.),which is a competitive P2X7 receptor antagonist,were injected one hour prior to TcdA *** samples were collected to analyze the expression of the P2X7 receptor(by quantitative real-time polymerase chain reaction and immunohistochemistry),the population of myenteric enteric neurons(immunofluorescence),histological damage,intestinal inflammation,cell death(terminal deoxynucleotidyltransferasemediated dUTP-biotin nick end labeling),neuronal loss,and S100B synthesis(immunohistochemistry).RESULTS TcdA upregulated(Pvel of this receptor in myenteric neurons compared to that in control *** with the control mice indicated that TcdA promoted(Pmyenteric calretinin+(Calr)and choline acetyltransferase+neurons and increased the number of nitrergic+and Calr+neurons expressing the P2X7 *** of the P2X7 receptor decreased TcdAinduced intestinal damage,cytokine release[interleukin(IL)-1β,IL-6,IL-8,and tumor necrosis factor-α],cell death,enteric neuron loss,and S100B synthesis in the mouse *** Our findings demonstrated that TcdA induced the upregulation of the P

关键词： Clostridioides difficile Clostridioides difficile toxin A P2X7 receptor Enteric nervous system Enteric neuron Enteric glia

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Clinical prognostic factors for disabling Crohn's disease: A systematic review and meta-analysis

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World Journal of Gastroenterology 2013年第24期19卷 3866-3871页

作者： Cláudia Camila Dias pedro Pereira Rodrigues Altamiro da costa Pereira Fernando magro Health Information and Decision Sciences Department Faculty of Medicine University of Porto4200-319 Porto Portugal Centre for Research in Health Informatics Systems and Technologies (CINTESIS) 4200-319 Porto Portugal Laboratory of Artificial Intelligence and Decision Support INESC TEC Artificial Intelligence and Decision Support Laboratory4200-465 Porto Portugal Centro Hospital de So Joo Institute of Pharmacology and Therapeutics Faculty of Medicine Univer-sity of Porto4200-319 Porto Portugal Institute for molecular and Cell Biology (IBmC) 4150-180 Porto Portugal

AIm: To identify demographic and clinical factors asso-ciated with disabling Crohn's disease (CD). mETHODS: A systematic review and meta-analysisof observational studies, focusing on the factors that can predict the prognosis of different outcomes of CD was undertaken. Pubmed, ISI Web of Knowledge and Scopus were searched to identify studies investigat-ing the above mentioned factors in adult patients with CD. Studies were eligible for inclusion if they describe prognostic factors in CD, with inclusion and exclusion criteria defined as follows. Studies with adult patients and CD, written in English and studying association between clinical factors and at least one prognosis out-come were included. meta-analysis of effects was un-dertaken for the disabling disease outcome, using odds ratio (OR) to assess the effect of the different factors in the outcome. The statistical method used was mantel-Haenszel for fixed effects. The 16-item quality assess-ment tool (QATSDD) was used to assess the quality of the studies (range: 0-42). RESULTS: Of the 913 papers initially selected, sixty studies were reviewed and three were included in the systematic review and meta-analysis. The global QA-TSDD scores of papers were 18, 21 and 22. Of a total of 1961 patients enrolled, 1332 (78%) were classified with disabling disease five years after diagnosis. In two studies, age at diagnosis was a factor associated with disabling disease five years after diagnosis. Individu-als under 40 years old had a higher risk of developing disabling disease. In two studies, patients who were treated with corticosteroids on the first flare developed disabling disease five years after diagnosis. Further, perianal disease was found to be relevant in all of the studies at two and five years after diagnosis. Finally, one study showed localization as a factor associated with disabling disease five years after diagnosis, with L3 being a higher risk factor. This meta-analysis showed a significantly higher risk

关键词： Crohn’s disease Disabling disease Prognos-tic factors Outcome Systematic review meta-analysis

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What is the value of eucalyptus monocultures for the biodiversity of the Atlantic forest? A multitaxa study in southern Bahia, Brazil

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Journal of Forestry Research 2013年第2期24卷 263-272页

作者： pedro Luís Bernardo da Rocha Blandina Felipe viana márcio Zikán Cardoso Amada mariana costa de melo misonete Gueidneli Cavalcanti costa Rodrigo Nogueira de vasconcelos Tatiana Bichara Dantas Instituto de Biologia Universidade Federal da Bahia. Rua Bar o de Jeremoabo

Eucalyptus plantations are increasing in Brazil, frequently replacing pastures, but there is still scarce information about its capacity to maintain the fauna of neighbor forest remnants. In this study, we compared descriptors of the communities of leaf litter organisms （lizards, anurans, myriapods, arachnids, orthopterans, coleopterans, and ants） between a large remnant of primary Atlantic Forest and an adjacent eucalyptus monoculture （phase 1）. Then, we compared the same descriptors for leaf litter lizards and anurans, Euglossini bees, and frugivorous butterflies among the largest remnant, small remnants at intermediate regeneration stage, and eucalyptus monocultures that were not adjacent to the largest remnant （phase 2）. monocultures were sampled immediately before logging. In phase 1, we detected significant differences in structure between the forest and the monoculture in six out of seven communities sampled. Ca. 81% of the species of the landscape were recorded in the forest, but only 54% of these were found also in the monoculture. In phase 2, the structure of two out of four forest communities was significantly different from the structure of small remnants and monocultures. On average, 76% of the species found in the whole landscape were sampled in the forest. Out of this subset, on average 74% of the species were also sampled in small remnants and 68% in monocultures. Findings of the present study point out a moderate capacity of eucalyptus monocultures to harbor species of the forest fauna even when fully grown but highlights the opportunity that they might offer for increasing connectivity in anthropogenic forest landscapes depending on their management.

关键词： Atlantic forest monoculture biodiversity

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Effect of increasing densities of Urochloa brizantha cv. marandu on Eucalyptus urograndis initial development in silvopastoral system

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Journal of Forestry Research 2019年第2期30卷 537-543页

作者： michel Anderson Almeida Colmanetti Allan Lopes Bacha pedro Luis da costa Aguiar Alves Rinaldo Cesar de Paula University of Sao Paulo – USP/ESALQ Sao Paulo State University – UNESP/FCAv

In silvopasture system, the coexistence of eucalyptus seedlings with other species may result in growth reduction, especially during eucalyptus early ***, studies elucidating how forage species affect the eucalyptus growth can provide important information for their rational management aiming to obtain the maximum gain of the system. The aim of this work was to evaluate the effect of increasing densities of Urochloa brizantha *** in the early development of Eucalyptus urograndis. An experiment was conducted in 20 L pots, in an open and semi-controlled area, during 90 days after planting of eucalyptus. A completely randomized design with four replications was used, in a 6 9 7 factorial system, meaning six evaluation periods and seven densities of U. brizantha: 0(control), 22, 33, 44, 67, 89 and111 plants m^(-2). Fortnightly, eucalyptus height, stem diameter and chlorophyll fluorescence(Fv/Fm) were evaluated. At the end of experimental period, the net assimilation rate, stomatal conductance and transpiration rate of eucalyptus plants were determined, in addition to the dry matter of eucalyptus(leaves and stem) and U. brizantha (leaves). In coexistence with 111 plants m^(-2), eucalyptus had reduction of 63.9% on total dry matter and 72.7% on leaf area, compared to the control. From the density of22 plants m^(-2), U. brizantha negatively interfere significantly the growth of E. urograndis. Up to 8 plants m^(-2) there are no reductions greater than 5% in eucalyptus height and stem diameter.

关键词： Eucalyptus Palisade grass Competition Interference

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How does high-intensity intermittent training affect recreational endurance runners? Acute and chronic adaptations: A systematic review

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Journal of Sport and Health Science 2017年第1期6卷 54-67页

作者： Felipe García-Pinillos víctor m.Soto-Hermoso pedro A.Latorre-Román Department of Corporal Expression University of Jaen Department of Sports Sciences University of Granada

Objective: This systematic review aimed to critically analyze the literature to determine how high-intensity intermittent training(HIIT) affects recreational endurance runners in the short-and ***: Electronic databases were searched for literature dating from January 2000 to October 2015. The search was conducted using the key words "high-intensity intermittent training" or "high-intensity interval exercise" or "interval running" or "sprint interval training" and "endurance runners" or "long distance runners". A systematic approach was used to evaluate the 783 articles identified for initial review. Studies were included if they investigated HIIT in recreational endurance runners. The methodological quality of the studies was evaluated using the Physiotherapy Evidence Database(pedro) scale(for intervention studies) and the modified Downs and Black Quality Index(for cross-sectional studies).Results: Twenty-three studies met the inclusionary criteria for review. The results are presented in 2 parts: cross-sectional(n = 15) and intervention studies(n = 8). In the 15 cross-sectional studies selected, endurance runners performed at least 1 HIIT protocol, and the acute impact on physiological, neuromuscular, metabolic and/or biomechanical variables was assessed. Intervention studies lasted a minimum of 4 weeks, with 10 weeks being the longest intervention period, and included 2 to 4 HIIT sessions per week. most of these studies combined HIIT sessions with continuous run(CR) sessions; 2 studies' subjects performed HIIT ***: HIIT-based running plans(2 to 3 HIIT sessions per week, combining HIIT and CR runs) show athletic performance improvements in endurance runners by improving maximal oxygen uptake and running economy along with muscular and metabolic adaptations. To maximize the adaptations to training, both HIIT and CR must be part of training programs for endurance runners.

关键词： Endurance High-intensity training Intermittent exercises Interval running Long-distance runners Running

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Assessing potential protection effects on commercial fish species in a Cuban mPA

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Aquaculture and Fisheries 2020年第5期5卷 234-244页

作者： Barbara Horta e costa Jorge Angulo-valdés Jorge m.S.Gonçalves pedro Barros Center of marine Sciences CCMARUniversity of AlgarveCampus de GambelasFaro8005-139Portugal Centro de Investigaciones marinas Universidad de La HabanaCalle 16 No.114PayaLa HabanaCP 11300Cuba School of Natural Resources and Environment University of FloridaUnited States of America103 Black HallGainesvilleFL32611USA Centre for marine and Environmental Research(CImA) University of AlgarveCampus de GambelasFaro8005-139Portugal Food and Agriculture Organization(FAO) Fisheries and Aquaculture Department Marine and Inland Fisheries Branch(FIRF)Rome00153Italy

Cuba has been leading marine protected area(mPA)designation in the Caribbean region to ensure conservation of its valuable marine ***,an efficient monitoring program for mPAs is still to be implemented and will benefit from pre-existing *** highly regulated mPA of Punta Francés National Park is one of the first Cuban mPAs and was established mainly to protect species and ecosystems for tourism *** of protection effects on commercial fish species was lacking in this *** study aimed at increasing local scientific knowledge by providing a baseline study about the most commercially fished families(Haemulidae,Lutjanidae and Serranidae)around Punta Francés *** collected represent only a limited period but can be used as a base point to support future *** abundance in number and biomass,and size were collected to test for differences between inside and adjacent areas outside the Punta Francés mPA,in different coral reef *** main result of this study was the significantly larger size and biomass of snappers and groupers outside the mPA where intense fisheries *** observation consistent with a large spawning aggregation was also recorded outside the *** with a limited set of data,these results suggest that,at least temporarily,the most targeted species[and sizes]are still highly vulnerable to *** habitats in the adjacent area,that are apparently missing within the mPA,may support some of the results *** the limits of the Punta Francés mPA to include those habitats outside may be critical to ensure the effectiveness of this mPA in contributing to protect the most commercial species of the *** should be done together with efficient fisheries management measures in the region,such as the significantly increase of minimum legal sizes and temporal closures during spawning migrations.

关键词： Cuba marine protected areas Commercial fish species Spawning aggregations Artisanal fisheries Coral reefs

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Recalcitrant cell wall of Ulva lactuca seaweed is degraded by a single ulvan lyase from family 25 of polysaccharide lyases

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Animal Nutrition 2022年第2期9卷 184-192页

作者： monica m.costa Luís B.Pio pedro Bule vania A.Cardoso marlene Duarte Cristina m.Alfaia Diogo F.Coelho Joana A.Bras Carlos m.G.A.Fontes Jose A.m.Prates CIISA-Centro de Investigaçao Interdisciplinar em Sanidade Animal Faculdade de Medicina Veterinari1Universidade de Lisbo1Alto da Ajud11300-477 Lisbo1Portugal NZYTech-Genes and Enzymes Estrada do Paço do LumiarCampus do LumiarEdifício E1649-038 Lisbo1Portugal

Green macroalgae,e.g.,Ulva lactuca,are valuable bioactive sources of nutrients;but algae recalcitrant cell walls,composed of a complex cross-linked matrix of polysaccharides,can compromise their utilization as feedstuffs for monogastric *** study aimed to evaluate the ability of pre-selected Carbohydrate-Active enZymes(CAZymes)and sulfatases to degrade *** cell walls and release nutritive compounds.A databank of 199 recombinant CAZymes and sulfatases was tested in vitro for their action towards *** cell wall *** enzymes were incubated with the macroalga,either alone or in combination,to release reducing sugars and decrease fluorescence intensity of Calcofluor White stained cell *** individual action of a polysaccharide lyase family 25(PL25),an ulvan lyase,was shown to be the most efficient in cell wall *** ulvan lyase treatment,in triplicate measures,promoted the release of 4.54 g/L(Pmono-and oligosaccharides release of 11.4 and 11.2 mmol/100 g of dried alga(Pvely,and a decrease of 41.7%(Pmparison to *** ability of ulvan lyase treatment to promote the release of nutritional compounds from alga biomass was also evaluated.A release of some monounsaturated fatty acids was observed,particularly the health beneficial 18:1c9(Pver,no significant release of total fatty acids(P>0.05),proteins(P?0.861)or pigments(P>0.05)was *** results highlight the capacity of a single recombinant ulvan lyase(PL25 family)to incompletely disrupt *** cell *** enzyme could enhance the bioaccessibility of *** bioactive products with promising utilization in the feed industry.

关键词： macroalga Ulva lactuca Carbohydrate-active enzyme Ulvan lyase Cell wall degradation

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