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Employing electrochemically derived pH gradients for Lab-on-PCB protein preconcentration devices

Microsystems & Nanoengineering 2024年第1期10卷 79-87页

作者： Grace Maxted pedro Estrela Despina Moschou Department of Electronic and Electrical Engineering University of BathBath BA27AYUK Centre for Bioengineering and Biomedical Technologies(CBio) University of BathBath BA27AYUK

Protein preconcentration is an essential sample preparation step for analysis in which the targeted proteins exist in low concentrations,such as bodily fluids,water,or ***,very few practical implementations of miniaturized protein preconcentration devices have been demonstrated in practice,and even fewer have been integrated with other microanalytical *** approaches rely heavily on additional chemicals and reagents and introduce complexity to the overall *** this paper,we propose a novel miniaturized isoelectric focusing-based protein preconcentration screening device based on electrochemically derived pH gradients rather than existing chemical reagent *** this way,we reduce the need for additional chemical reagents to zero while enabling device incorporation in a seamlessly integrated full protein analysis microsystem via Lab-on-PCB *** apply our previously presented Lab-on-PCB approach to quantitatively control the pH of a solution in the vicinity of planar electrodes using electrochemical acid generation through redox-active self-assembled *** presented device comprises a printed circuit board with an array of gold electrodes that were functionalized with 4-aminothiophenol;this formed a self-assembled monolayer that was electropolymerized to improve its electrochemical *** preconcentration was performed in two *** first was open and needed the use of a holder to suspend a well of fluid above the electrodes;the second used microfluidic channels to enclose small volumes of *** here are the resulting data for protein preconcentration in both these forms,with a quantitative concentration factor shown for the open form and qualitative proof shown for the microfluidic.

关键词： electrochemical proof qualitative

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Conformational dynamics as an intrinsic determinant of prion protein misfolding and neurotoxicity

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Neural Regeneration Research 2024年第10期19卷 2095-2096页

作者： Alessandro Cembran pedro Fernandez-Funez Department of Chemistry and Biochemistry University of Minnesota DuluthDuluthMNUSA Department of Biomedical Sciences University of Minnesota Medical SchoolDuluth CampusDuluthMNUSA

The prion protein(PrP) is the key molecular and pathological mediator of prion diseases,a heterogeneous group of brain disorders with fatal *** diseases are rare but deserve special attention because of their unique f... 详细信息

关键词： folding intrinsic dynamics

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Effect of the Coulomb and dielectric forces on the onset of Rayleigh-Bénard convection

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Acta Mechanica Sinica 2024年第6期40卷 64-77页

作者： Zhonglin Du pedro A.Vázquez Jian Wu School of Energy Science and Engineering Harbin Institute of TechnologyHarbin150001China Heilongjiang Key Laboratory of Micro-and Nano-scale Fluid Flow and Heat Transfer Harbin150001China Departamento de Física Aplicada III ETSIIUniversidad de SevillaSevilla41092Spain

In this paper,the effect of Coulomb and dielectric forces on the onset of Rayleigh-Bénard convection(RBC)in a dielectric liquid layer contained between two parallel electrode plates has been numerically *** different operating conditions have been considered in electrohydrodynamic(EHD)conduction:ohmic and saturation,and variations in the physical properties of the dielectric fluid with *** electric equations and the state equations based on the Boussinesq approximation are integrated in the framework of OpenFOAM buoyantBoussinesqPimpleFoam *** results show that in the ohmic regime,the combined effect of Coulomb and dielectric forces promotes the onset of RBC flow,while in the saturation state,the inhibition of RBC flow by Coulomb force is more *** value of the critical Rayleigh number Ra decreases with increasing electric Reynolds number ReE in the ohmic regime,whereas in the saturation state,the critical Ra increases with increasing *** the saturation regime,the flow field always has a steady flow in the range of parameters ***,the onset of the RBC flow promoted by the dielectric force is more pronounced in the ohmic *** to the presence of the dielectric force,the flow field exhibits periodic oscillatory flow at low electric Reynolds numbers for the range of parameters considered.

关键词： EHD conduction Dielectric force Rayleigh-Bénard convection OpenFOAM

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Inversion of walkaway VSP data in the presence of lateral velocity heterogeneity

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Energy Geoscience 2024年第2期5卷 304-313页

作者： Vladimir Grechka Ilya Tsvankin pedro Contreras CWP Department of GeophysicsColorado School of MinesGoldenCO80401USA PDVSA-INTEVEP Apartado 76343Caracas1070AVenezuela Borehole Seismic LLC USA Department of Physics ULAVenezuela

Multi-azimuth walkaway vertical seismic profiling is an established technique for the estimation of in situ slowness surfaces and inferring anisotropy ***,this technique requires the assumption of lateral homogeneity,which makes the horizontal slowness components at depths of downhole receivers equal to those measured at the *** violations of this assumption,such as lateral heterogeneity or nonzero dip of intermediate interfaces,lead to distortions in reconstructed slowness surfaces and,consequently,to errors in estimated anisotropic *** this work,we relax the assumption of lateral homogeneity and discuss how to correct vertical seismic profile data for weak lateral *** describe a procedure of downward continuation of recorded traveltimes that accounts for the presence of both vertical inhomogeneity and weak lateral heterogeneity,which produces correct slowness surfaces at depths of downhole receivers,noticing that sufficiently dense receiver coverage along a borehole is required to separate influences of vertical and lateral heterogeneity on measured traveltimes and obtain accurate estimates of the slowness *** the slowness surfaces are found and a desired type of anisotropic model to be inverted is selected,the corresponding anisotropic parameters,providing the best fit to the estimated slownesses,can be *** invert the slowness surfaces of P-waves for parameters of the simplest anisotropic model describing dipping fractures(transversely isotropic medium with a tilted symmetry axis).Five parameters of this model,namely,the P-wave velocity V0 in the direction of the symmetry axis,Thomsen's anisotropic coefficients e and d,the tilt n,and the azimuth b of the symmetry axis,can be estimated in a stable manner when maximum source offset is greater than half of receiver depth.

关键词： Walkaway vertical seismic profile Lateral velocity heterogeneity Anisotropic parameter estimation Vertical inhomogeneity

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New pharmacological tools:the use of diterpenes to promote adult hippocampal neurogenesis

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Neural Regeneration Research 2024年第8期19卷 1629-1630页

作者： Ricardo Gomez-Oliva pedro Nunez-Abades Carmen Castro Department of Biomedicine Biotechnology and Public HealthDivision of PhysiologyUniversity of CadizCadizSpain Biomedical Research and Innovation Institute of Cadiz(INiBICA) CadizSpain Department of Physiology University of SevilleSevilleSpain

Tissue regeneration maintains homeostasis and preserves the functional features of each ***,not all tissues show a strong repairing *** is the case of the central nervous *** is now well established that the generation of new functional neurons from stem cells in the adult brain occurs in specific regions of the brain of different species such as rodents,birds,primates,and humans(Eriksson et al.,1998).

关键词： capacity maintain preserve

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Ergonovine stress echocardiography: Recent experience and safety in our centre

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World Journal of Cardiology 2010年第12期2卷 437-442页

作者： Alejandro Cortell pedro marcos-alberca Carlos Almería JoséL Rodrigo Leopoldo Pérez-Isla Carlos Macaya JoséLuis Zamorano University Clinic San Carlos Cardiovascular InstituteProfesor Martín Lagos Street

AIM:To study recent experience and safety of ergonovine stress echocardiography in our ***:In this study we collected the clinical variables of patients referred since 2002 for ergonovine stress echocardiography,in addition to indications,the results of this test,complications,blood pressure and heart rate values during the test and the number and results of tests requested before this ***:We performed 40 tests in 38 patients,2 tests were carried out to verify therapy *** prevalence of classic cardiovascular risk factors was low and the most frequent indication was chest pain (57.5%).Coronary angiography was performed in 32 patients,and showed normal coronary arteries in 27 patients and non-significant stenosis in 5 *** 16 patients,coronary angiography was carried out after a positive or inconclusive ischemia *** 6 patients had a normal stress test(5 exercise electrocardiography tests and 1 nuclear imaging test).Of the 40 ergonovine stress echocardiography tests,6 were positive(4 in the right coronary artery territory and 2 in the circumflex coronary artery territory),all of them by echocardiographic criteria,and by electrocardiographic criteria in only 3(50%).The presence of non-significant coronary artery stenosis was more frequent in patients with positive ergonovine stress echocardiography(50%vs 6%,P =0.038),and were related to ischemic *** the maximum stress stage,there was a higher systolic(130.26±19.17 mmHg vs 136.58±27.27 mmHg,95%CI:-12.77 to 0.14 mmHg,P=0.055)and diastolic blood pressure(77.89±13.49 mmHg vs 83.95± 15.73 mmHg,95%CI:-10.41 to-1.69 mmHg,P=0.008) than at the baseline stage,and the same was registered with heart rate(73±10.96 beats/minvs 79.79± 11.72 beats/min,95%CI:-9.46 to-4.11 beats/min,P <0.01).Nevertheless,there were only 2 hypertensive reactions during the last stage,which did not force a premature end to the test,without sustained tachy or bradyarrhythmias,and the technique was

关键词： Coronary angiography Ergonovine Myocardial ischaemia Stress echocardiography Vasospasm

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Targeting epidermal growth factor receptor signaling to facilitate cortical injury repair?

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Neural Regeneration Research 2024年第5期19卷 935-936页

作者： Ricardo Gómez-Oliva pedro Nunez-Abades Carmen Castro Área de Fisiología Facultad de Medicina Universidad de CádizCadizSpain Instituto de Investigación e Innovación en Biomedicina de Cádiz CadizSpain Departamento de Fisiología Facultad de FarmaciaUniversidad de SevillaSevilleSpain

The existence of neural stem cells(NSC)in specific areas of the adult mammalian brain leads to the generation of new neurons involved in homeostatic *** is the case of the NSC of the subventricular zone(SVZ)that produces olfactory bulb(OB)*** neurons integrate into circuits to modulate sensory information and participate in olfactory processing(Bragado Alonso et al.,2019).A series of hierarchical events need to take place to produce OB neurons from NSC of the ***,the activation of quiescent NSC is necessary to produce intermediate transit amplifying progenitors(TAP),which will generate *** latter are cells that maintain a proliferative phenotype while in an immature ***,once they initiate migration toward the OB,they progressively undergo their differentiation into neurons(Kjell et al.,2020).

关键词： ventricular maintain

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Assessment of energy consumption,environmental effects and fuel costs of the bus rapid transit system in Bogotá(Colombia)

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Clean Energy 2024年第3期8卷 34-47页

作者： Jhon Jairo Pérez Luis Hernando Correa Poul AlbergØstergaard pedro Cabrera Universidad de La Salle-Engineering Faculty Electrical DepartmentCampus La CandelariaBogota D.C.Colombia Aalborg University Department of PlanningRendsburggade 149000 AalborgDenmark University of Las Palmas de Gran Canaria Department of Mechanical EngineeringCampus Universitario de TafiraLas Palmas de Gran CanariaSpain

Colombia aims to boost the utilization of mass transportation systems in its major cities while simultaneously reducing greenhouse gas emissions by 20%,in alignment with the commitments of the COP21 *** 2020,the transport sector in Colombia accounted for 34.4%of the country’s energy demand and was responsible for~49%of its total CO_(2) *** article presents an assessment of energy consumption,environmental effects and the fuel costs of Bogotá’s bus rapid transit system based on the Activity,Share,Intensity,Fuel methodology.A long-term analysis spanning from 2021 to 2040 was developed using the long-range energy alternatives planning *** conduct this assessment,the tool was calibrated using data from 2019 and *** distinct scenarios based on energy policies implemented in Bogotáwere examined:Business as Usual,Fast Transition,High Growth and Low *** energy consumption and environmental effects,the results underscore the pivotal role of diversifying energy sources and reducing reliance on fossil fuels such as ***,the analysis emphasizes the urgent need to accelerate the transition to alternative energy sources such as natural gas and electricity.

关键词： ASIF energy consumption environmental effects LEAP long-term energy model public transport

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Exploring the size of Andean condor foraging groups along an altitudinal and latitudinal gradient in the Tropical Andes: Ecological and conservation implications

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Current Zoology 2024年第2期70卷 225-232页

作者： Diego Mendez Felix Hernan Vargas JoséHernan Sarasola pedro P.Oleaa Departamento de Ecologia Universidad Autonoma de MadridCalle Darwin 228049MadridSpain Museo Nacional de Historia Natural Calle 26 s/nCota CotaLa PazBolivia The Peregrine Fund 5668 West Flying Hawk LaneBoiseID 83709USA Centro para el Estudio y Conservacion de Aves Rapaces en Argentina(CECARA) Universidad Nacional de La PampaAvda Uruguay 1516300 Santa RosaLa PampaArgentina Instituto de las Ciencias Ambientales y de la Tierra de La Pampa(INCITAP-CONICET) Avda.Uruguay 1516300 Santa RosaLa PampaArgentina Centro de Investigacion en Biodiversidad y Cambio Global(CIBC-UAM) Universidad Autonoma de MadridMadridCalle Darwin 228049Madrid Snain

Patterns of variation in the size of vulture foraging groups,and their ecological causes and consequences,remain litle explored despite strong links with the carrion recycling service that this key functional group *** documented the group size-frequency pattern of Andean con-dors Vultur gryphus gathered to feed on 42 equine carcasses experimentally placed in Bolivia,between 2012 and 2019,along an elevation range of 1,300-4,500 m *** on the location(altitude and latitude)of the foraging groups,we examined the relationship between their size and species'population parameters(size and trend),habitat conditions,and livestock carcass availability and *** utilized a high frequency(93%)of carcasses forming groups that ranged from 1 to 80 individuals(mean=25,median=18)and shaped a"lazy-J curve"typical pattern of size-frequency distribution whereby few groups(5,12%)were large(>55 individuals)and most(21,50%)were relatively small(<19 individuals).Group size related to altitude in that most larger groups formed at lower sites(below c.3,000 m asl),likely following an altitudinal gradient whereby larger groups are more likely to form around larger carcasses(i.e.,cattle),which are more likely to occur at lower *** of population size,group size could be an adaptive response of condors via local enhancement for improving individual scavenging *** information gaps on this topic still exist,thus we provide a set of questions to address them,especially amidst the unrestricted impacts of human activities that condition vulture survival globally.

关键词： condor andino fusion-fission dynamics group size scavenger social foraging vultures.

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Technical efficiency of cocoa farms at varying elevation levels in Davao City, Philippines: implications to sustainable upland farming systems

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Journal of Mountain Science 2024年第1期21卷 33-48页

作者： Francis Levi A.DURANO Jon Marx SARMIENTO Larry N.DIGAL pedro A.ALVIOLA IV School of Management University of the Philippines MindanaoDavao City 8000Philippines

Elevation is one of many components that influence agriculture, and this in turn affects the level of both inputs and outputs of farmers. This article focuses on the productivity and technical efficiency of 100 cocoa farms using cross-sectional data from areas ranging from 190 to 1021 m above sea level which were classified as low, medium, and high elevation in Davao City, considered as the chocolate capital of the Philippines. Using stochastic frontier analysis, the results showed that the cost of inputs per ha and the number of cocoa trees per ha significantly increase yield. Farms at high elevations were less technically efficient, as this entails lower temperatures and increased rainfall, and cocoa farming in those areas and conditions can be more challenging, especially with changes in farming practices, terrain, and distance to markets. Other significant variables were age of cocoa farms, married farmers, and age of the farmers. Older farms may be more developed, farmers who are married benefit from their spouses being able to readily contribute as farm labor, and lastly, older farmers' inefficiency may likely stem from nonadaptation of newer farming practices. With an average technical efficiency of 0.61, 0.63, and 0.26 in low, medium, and high elevation areas, respectively, farmers therefore have an incentive to improve farm practices and consider topographical variations found in high elevation areas. Recommendations for the improvement of technical efficiency of cocoa farms are better connectivity to markets, enhancing farm practices, and continuation and improvement of government programs on cocoa with an added emphasis on research. For farmers in high elevation areas, mitigating solutions such as sustainable agriculture practices and ecolabelling are key to improving efficiency and minimizing the potential negative impact on upland farming systems. Moreover, such adaptation measures may also contribute to sustainability of cocoa farming in high elevation

关键词： Cocoa farms Cobb-Douglas production function Elevation Philippines Stochastic frontier analysis Technical efficiency

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