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QTL analysis for some quantitative traits in bread wheat

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2007年第11期8卷 807-814页

作者： PUSHPENDRA Kumar Gupta HARINDRA Singh Balyan pawan laxminarayan kulwal NEERAJ Kumar AJAY Kumar REYAZUL Rouf Mir AMITA Mohan JITENDRA Kumar Molecular Biology Laboratory Department of Genetics and Plant BreedingChaudhary Charan Singh UniversityMeerut 250 004India

Quantitative trait loci (QTL) analysis was conducted in bread wheat for 14 important traits utilizing data from four different mapping populations involving different approaches of QTL analysis. Analysis for grain protein content (GPC) sug- gested that the major part of genetic variation for this trait is due to environmental interactions. In contrast, pre-harvest sprouting tolerance (PHST) was controlled mainly by main effect QTL (M-QTL) with very little genetic variation due to environmental interactions; a major QTL for PHST was detected on chromosome arm 3AL. For grain weight, one QTL each was detected on chromosome arms 1AS, 2BS and 7AS. QTL for 4 growth related traits taken together detected by different methods ranged from 37 to 40; nine QTL that were detected by single-locus as well as two-locus analyses were all M-QTL. Similarly, single-locus and two-locus QTL analyses for seven yield and yield contributing traits in two populations respectively allowed detection of 25 and 50 QTL by composite interval mapping (CIM), 16 and 25 QTL by multiple-trait composite interval mapping (MCIM) and 38 and 37 QTL by two-locus analyses. These studies should prove useful in QTL cloning and wheat improvement through marker aided selection.

关键词： Quantitative trait loci (QTL) analysis Grain quality traits Grain protein content Pre-harvest sprouting tolerance(PHST) Grain weight Mapping populations Bread wheat

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Genetic Diversity and Population Structure in Landraces and Improved Rice Varieties from India

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Rice science 2015年第3期22卷 99-107页

作者： Shailesh D.KUMBHAR pawan L.kulwal Jagannath V.PATIL Chandrakant D.SARAWATE Anil P.GAIKWAD Ashok S.JADHAV State Level Biotechnology Centre Mahatma Phule Agricultural University Agricultural Research Station Directorate of Sorghum Research

A set of 50 rice genotypes comprising landraces, local selections, and improved varieties were characterized using simple sequence repeat（SSR） and inter simple sequence repeat（ISSR） markers to study genetic diversity and population structure. Following unweighted pair group method with arithmetic mean based clustering using binary data of polymorphic markers, the genotypes were grouped into 5 clusters and 11 sub-clusters, whereas population structure analysis separated 50 rice genotypes into 5 sub-populations. Grouping of rice genotypes showed better resemblance with the pedigree information of the genotypes. Both genetic diversity and population structure analysis separated majority of the improved varieties from landraces and local selections. Some of the SSR markers amplified unique alleles which were specific to a particular genotype and could distinguish them from the rest. The results indicate that these rice genotypes exhibit a higher genetic diversity and can be very useful in rice improvement program.

关键词： rice genetic diversity molecular marker population structure simple sequence repeat

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