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Using Optical Tweezers to Study the Friction of the Red Blood Cells

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Advances in Bioscience and Biotechnology 2024年第2期15卷 100-111页

作者： Edoukoua Jean Michel Konin pavel Yale Abadê Ange-Boris N’guessan Kouassi Benoit Kouakou Abaka Michel Kouacou Eugene Megnassan Département des Sciences et Techniques Université Alassane Ouattara Bouaké C&#244te d’Ivoire Laboratoire d’Instrumentation Image et Spectroscopie (L2IS) Institut National Polytechnique Félix Houphou&#235t-Boigny (INPHB) Yamoussoukro C&#244te d’Ivoire Classes préparatoires aux grandes Ecoles Université de San Pedro San Pedro C&#244te d’Ivoire Laboratoire de Physique Fondamentale et Appliquée Université Nangui Abrogoua (UNA) Abidjan C&#244te d’Ivoire

In the last two decades the study of red blood cell elasticity using optical tweezers has known a rise appearing in the scientific research with regard to the various works carried out. Despite the various work done, no study has been done so far to study the influence of friction on the red blood cell indentation response using optical tweezers. In this study, we have developed a new approach to determine the coefficient of friction as well as the frictional forces of the red blood cell. This approach therefore allowed us to simultaneously carry out the indentation and traction test, which allowed us to extract the interfacial properties of the microbead red blood cell couple, among other things, the friction coefficient. This property would be extremely important to investigate the survival and mechanical features of cells, which will be of great physiological and pathological significance. But taking into account the hypothesis of friction as defined by the isotropic Coulomb law. The experiment performed for this purpose is the Brinell Hardness Test (DB).

关键词： Friction Indentation Optical Tweezers Tribology Red Blood Cells

Human Eosinophil Cell Manipulation by Optical Tweezers

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Open Journal of Biophysics 2024年第3期14卷 330-338页

作者： pavel Yale Jean Michel Edoukoua Konin Abadê Ange-Boris N’guessan Michel Abaka Kouacou Jérémie T. Zoueu Laboratoire dInstrumentation Image et Spectroscopie (L2IS) Institut National Polytechnique Houphout-Boigny (INPHB) Yamoussoukro Cte dIvoire Laboratoire de Chimie et de Physique Appliques Sciences et Techniques Universit Alassane Ouattara Bouak Cte dIvoire

In this work, lateral deformation of human eosinophil cell during the lateral indentation by an optically trapped microbead of diameter 4.5 µm is studied. The images were captured using a CCD camera and the Boltzmann statistics method was used for force calibration. Using the Hertz model, we calculated and compared the elastic moduli resulting from the lateral force, showing that the differences are important and the force should be considered. Besides the lateral component, the setup also allows us to examine the lateral cell-bead interaction. The mean values of the properties obtained, in particular the elastic stiffness and the shear stiffness, were Eh = (37.76 ± 2.85) µN/m and Gh = (12.57 ± 0.32) µN/m. These results show that the lateral indentation can therefore be used as a routine method for cell study, because it enabled us to manipulate the cell without contact with the laser.

关键词： Optical Tweezers Human Eosinophil Cell Indentation Shear Stiffness

Fully integrated and broadband Si-rich silicon nitride wavelength converter based on Bragg scattering intermodal four-wave mixing

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Photonics Research 2024年第3期12卷 I0001-I0010页

作者： VALERIO VITALI THALÍA DOMÍNGUEZ BUCIO HAO LIU JOSÉMANUEL LUQUE GONZÁLEZ FRANCISCO JURADO-ROMERO ALEJANDRO ORTEGA-MOÑUX GLENN CHURCHILL JAMES C.GATES JAMES HILLIER NIKOLAOS KALFAGIANNIS DANIELE MELATI JENS H.SCHMID ILARIA CRISTIANI pavel CHEBEN J.GONZALO WANGÜEMERT-PÉREZ ÑIGO MOLINA-FERNÁNDEZ FREDERIC GARDES COSIMO LACAVA PERIKLIS PETROPOULOS Optoelectronics Research Centre University of SouthamptonSouthampton SO171BJUK Electrical Computer and Biomedical Engineering DepartmentUniversity of PaviaPavia 27100Italy Telecommunication Research Institute(TELMA) Universidad de MálagaCEI Andalucı´a TECHE.T.S.I.Telecomunicación29010 MálagaSpain School of Science and Technology Nottingham Trent UniversityNottingham NG118NSUK Department of Electrical Engineering Eindhoven University of TechnologyEindhoven 5600 MBThe Netherlands Department of Materials Science and Engineering University of IoanninaIoannina 45110Greece Centre de Nanosciences et de Nanotechnologies UniversitéParis-SaclayCNRS91120 PalaiseauFrance Advanced Electronics and Photonics Research Center National Research Council CanadaOttawaOntario K1A 0R6Canada

Intermodal four-wave mixing(FWM)processes have recently attracted significant interest for all-optical signal processing applications thanks to the possibility to control the propagation properties of waves exciting distinct spatial modes of the same *** allows,in principle,to place signals in different spectral regions and satisfy the phase matching condition over considerably larger bandwidths compared to intramodal ***,the demonstrations reported so far have shown a limited bandwidth and suffered from the lack of on-chip components designed for broadband manipulation of different *** demonstrate here a silicon-rich silicon nitride wavelength converter based on Bragg scattering intermodal FWM,which integrates mode conversion,multiplexing and de-multiplexing functionalities *** system enables wavelength conversion between pump waves and a signal located in different telecommunication bands(separated by 60 nm)with a 3 dB bandwidth exceeding 70 nm,which represents,to our knowledge,the widest bandwidth ever achieved in an intermodal FWM-based system.

关键词： scattering pump modal

Interaction of water droplets with pyrolyzing coal particles and tablets

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Defence Technology（防务技术） 2023年第12期30卷 1-12页

作者： Anastasia Islamova pavel Tkachenko pavel Strizhak National Research Tomsk Polytechnic University Heat and Mass Transfer Laboratory30 Lenin AvenueTomsk634050Russia

The paper presents the experimental research findings for the patterns of collisions of water droplets with pressed tableted samples used as substrates and with small particles of a pyrolyzing solid *** coal samples were ***-substrate interactions were studied when varying the droplet diameter in the range from 1 to 4 mm and velocity from 0.5 to 4 m/*** corresponded to the Weber number range of *** coal tablet surface temperature was varied from 20 to 700℃.In the interactions of water droplets(0.7-1.5 mm in diameter,pre-collision velocity from 1 to 3 m/s)with coal particles(with a size of 0.2-1 mm,pre-collision velocity 0.7-2 m/s),the temperature of the latter was varied in the range of 330-480℃.The following regimes of the interaction of droplets with solid particles during chemical reactions and phase transformations were distinguished:spreading/agglomeration and break-up/*** in the characteristics of the interaction of water droplets with coal particles at varying temperatures were ***-particle interaction regime maps for B(We),We(Oh)and We(Ca)were *** collision regime boundaries were described using fitted curves that can be utilized to develop the existing mathematical models of droplet-particle collisions in *** was established that the gaseous volatile production in coal pyrolysis has a modest effect on the regimes and characteristics of the droplet destruction in the temperature range under consideration(20-700℃).

关键词： Droplet Particle Solid substrate Collision Agglomeration Separation

Influence of stress path change on the resistance to plastic deformation of cold rolled sheets

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Journal of University of Science and Technology Beijing 2005年第6期12卷 521-526页

作者： pavel Huml Metal Forming Department of Materials Science and Engineering The Royal Institute of Technology 10044 Stockholm Sweden

Flat workpieces have been tested in order to investigate the influence of stress path change （loading mode） while keeping strain path unchanged. These investigations are pertinent to the testing of cold rolled strips and to subsequent forming. The workpieces which first compressed by plane strain compression in thickness direction were then tested in perpendicular direction in order to measure the influence of strain and stress path. The tension workpieces came from flat die compression test at different deformation histories. Two different materials were investigated： 18/8 Ti stainless steel and AW-1050 aluminium. The results show that the plastic flow by tension in lengthwise direction after pre-strain by compression in thickness direction will begin at an appreciably lower stress than that of the workpieces unloaded after pre-compression. Comparing with two materials, it can be seen that both 18/8 Ti stainless steel and AW-1050 aluminium behave similarly. The drop in yield stress is lower for AW-1050 aluminium than that for 18/8 Ti stainless steel. However, reloading in different directions than in the precious step results in significantly higher strain hardening.

关键词： cold working plastic deformation strain path stress path loading mode flow stress yield stress strain hardening

葡萄VvMET1-1基因在葡萄和拟南芥中的功能研究

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中外葡萄与葡萄酒 2024年第3期 1-12页

作者：王风霞丁亭亭刘蕙萍李科杨国伟李傲张庆田 Chebukin pavel 任凤山王鹏飞山东省葡萄研究院山东济南250100 俄罗斯瓦维洛夫植物研究所海参崴690014 山东省农业科学院山东济南250100 山东省酿酒葡萄与葡萄酒技术创新中心山东烟台265600

DNA甲基化修饰对于植物的生长发育十分重要,而MET1是植物中重要的甲基转移酶,能维持植物的CG位点甲基化修饰。为了研究葡萄MET1基因的功能及对植物发育的影响,将葡萄VvMET1-1基因转入葡萄胚性愈伤组织和拟南芥中进行研究。本研究选择35S... 详细信息

DNA甲基化修饰对于植物的生长发育十分重要,而MET1是植物中重要的甲基转移酶,能维持植物的CG位点甲基化修饰。为了研究葡萄MET1基因的功能及对植物发育的影响,将葡萄VvMET1-1基因转入葡萄胚性愈伤组织和拟南芥中进行研究。本研究选择35S::VvMET1-1-GFP表达载体转入葡萄胚性愈伤组织后检测到明显的GFP信号,且该胚性愈伤组织能被成功诱导为体细胞胚,表明VvMET1-1首次被成功转入葡萄胚性愈伤组织中并且不影响胚性愈伤组织的发育和分化。VvMET1-1被转入拟南芥中发现,该基因能影响拟南芥叶片的长度和宽度,但是不影响植株形态和种子发育;转录组数据分析表明,许多逆境胁迫、生长素、种子萌发、果实成熟、脱落酸和茉莉酸合成相关的基因差异表达;甲基化组结果显示,拟南芥过表达VvMET1-1后CG和CHH位点甲基化水平分别发生了明显的上调和下调,且DNA甲基化差异区(DMR)的类型主要是CG和CHH型,因此推测VvMET1-1可能通过调控DNA甲基化水平的变化影响下游基因的表达,进而调控拟南芥的生长发育。

关键词：葡萄胚性愈伤组织 VvMET1-1 转录组测序

Exploring Antarctic subglacial lakes with RECoverable Autonomous Sonde(RECAS):Design and first field tests

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Science China(Technological Sciences) 2024年第6期67卷 1866-1878页

作者： SUN YouHong pavel Talalay LI YuanSheng YU HaiBin WANG DongLiang LI GuoPing XU LiPing GONG Da WANG JiXin WANG JianHua WANG Ting ZHANG Nan WANG ZhiGang CHEN YanJi LIU YunChen LI YaZhou PENG ShiLin SHI JianGuang AN ChunLei GE Qun XU Jing NI XiaoKang CUI QiFeng JIANG Qiang MIKHAIL Sysoev Yang Yang WANG RuSheng WEI XianZhe WANG Yu ZHU TianXin DENG ZhiPeng ALEXEY Markov LI Bing FAN XiaoPeng Polar Research Center Jilin UniversityChangchun130000China School of Engineering and Technology China University of GeosciencesBeijing100083China Polar Research Institute of China Shanghai200120China College of Electronics and Information Hangzhou Dianzi UniversityHangzhou310018China Aerospace System Engineering Shanghai Shanghai201109China Nanjing Institute of Astronomical Optics&Technology National Astronomical ObservatoriesChinese Academy of SciencesNanjing210000China National Ocean Technology Center Tianjin300000China

Conducting scientific drilling on subglacial lakes and obtaining samples of subglacial lake water holds great significance in unraveling the formation and evolution of Antarctic subglacial lakes and early Earth's life *** various approaches to access and directly sample subglacial water and sediments,clean access and exploration of subglacial lakes remain *** address this concern,Jilin University has developed the RECoverable Autonomous Sonde(RECAS)*** technology enables sampling and in-situ detection of subglacial lake water while being isolated from the surface,thus minimizing the risk of *** tests,including downward and upward drilling,long-running,remote-control,and cold-environment assessments,were conducted to validate the sonde's principle and *** the 38th Chinese National Antarctic Research Expedition,CHINARE(2021–2022 season),the RECAS prototype underwent testing on the flank region of Dalk glacier,10 km from Zhongshan Station in *** boreholes with depths of 200.3,183.2,and 133.5 m were successfully drilled,with the refrozen meltwater sealing the boreholes during the *** 600 mL of melted water samples were collected from each *** the drilling tests,all systems of the RECAS prototype performed within the expected ranges.

关键词： subglacial lake thermal drilling water sampling Antarctic autonomous sonde

Evaluation of the seismic hazard for 20 cities in Romania using Monte Carlo based simulations

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Earthquake Engineering and Engineering Vibration 2017年第3期16卷 513-523页

作者： Florin pavel Radu Vacareanu Seismic Risk Assessment Research Center Technical University of Civil Engineering Bucharest Romania

This work focuses on the evaluation of the seismic hazard for Romania using earthquake catalogues generated by a Monte Carlo approach. The seismicity of Romania can be attributed to the Vrancea intermediate-depth seismic source and to 13 other crustal seismic sources. The recurrence times of large magnitude seismic events（both crustal and subcrustal）, as well as the moment release rates are computed using simulated earthquake catalogues. The results show that the largest contribution to the overall moment release for the crustal seismic sources is from the seismic regions in Bulgaria, while the seismic regions in Romania contribute less than 5% of the overall moment release. In addition, the computations show that the moment release rate for the Vrancea subcrustal seismic source is about ten times larger than that of all the crustal seismic sources. Finally, the Monte Carlo approach is used to evaluate the seismic hazard for 20 cities in Romania with populations larger than 100,000 inhabitants. The results show some differences between the seismic hazard values obtained through Monte-Carlo simulation and those in the Romanian seismic design code P100-1/2013, notably for cities situated in the western part of Romania that are influenced by local crustal seismic sources.

关键词： seismic sources uncertainty seismic moment GMPE percentile

Prognosis of residual coal gas capacity made by the ‘Express' method

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International Journal of Minerals,Metallurgy and Materials 2011年第2期18卷 127-131页

作者： pavel Prokop pavel Zapletal Ivo Pěgímek Technical University of Ostrava

An easy, reliable, and inexpensive method, called ＇Express＇ method, was described to determine the residual gas capacity of deep mines using results from an air and gas balance. Air and gas balances are common elements of mine management and must be performed periodically. Using the process described here to obtain balance results, it is straightforward to obtain the residual gas capacity, which is an important parameter for decision-making in current mine operations. After a mine is closed, the residual gas capacity becomes a dominant factor used to select methods to protect against gas emissions from the closed underground area or perhaps to provide information for the use of gas reserves. The proposed ＇Express＇ method is a much simpler method to obtain the residual gas capacity than other procedures used for this purpose to date.

关键词： coal mines gas storage methane ventilation degassing