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Zinc phosphate-based nanoparticles as a novel antibacterial agent: in vivo study on rats after dietary exposure

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Journal of Animal Science and biotechnology 2019年第2期10卷 463-474页

作者： pavel Horky Sylvie Skalickova Lenka Urbankova Daria baholet Silvia Kociova Zuzana bytesnikova Eliska Kabourkova Zuzana Lackova Natalia Cernei Milica Gagic Vedran Milosavljevic Vendula Smolikova Eva Vaclavkova pavel Nevrkla pavel Knot Olga Krystofova David Hynek pavel Kopel Jiri Skladanka Vojtech Adam Kristyna Smerkova Department of Animal Nutrition and Forage Production Mendel University in BrnoZemedelska 1CZ-613 00 BrnoCzech Republic Department of Chemistry and biochemistry Mendel University in BrnoZemedelska 1CZ-613 00 BrnoCzech Republic Central European Institute of Technology Brno University of TechnologyPurkynova 123CZ-612 00 BrnoCzech Republic Institute of Animal Science Komenskeho 1239CZ-517 41 Kostelec nad OrliciCzech Republic Department of Animal breeding Mendel University in BrnoZemedelska 1CZ-613 00 BrnoCzech Republic.

background: Development of new nanomaterials that inhibit or kil bacteria is an important and timely research topic. For example, financial losses due to infectious diseases, such as diarrhea, are a major concern in livestock productions around the world. Antimicrobial nanoparticles(NPs) represent a promising alternative to antibiotics and may lower antibiotic use and consequently spread of antibiotic resistance traits among bacteria, including ***: Four formulations of zinc nanoparticles(Zn A, Zn b, Zn C, and Zn D) based on phosphates with spherical(Zn A, Zn b)or irregular(Zn C, Zn D) morphology were prepared. The highest in vitro inhibitory effect of our NPs was observed against Staphylococcus aureus(inhibitory concentration values, IC50, ranged from 0.5 to 1.6 mmol/L), fol owed by Escherichia coli(IC500.8–1.5 mmol/L). In contrast, methicil in resistant S. aureus(IC501.2–4.7 mmol/L) was least affected and this was similar to inhibitory patterns of commercial Zn O-based NPs and Zn O. After the successful in vitro testing, the in vivo study with rats based on dietary supplementation with zinc NPs was conducted. Four groups of rats were treated by 2,000 mg Zn/kg diet of Zn A, Zn b, Zn C, and Zn D, for comparison two groups were supplemented by 2,000 mg Zn/kg diet of Zn O-N and Zn O, and one group(control) was fed only by basal diet. The significantly higher(P bic and coliform bacterial population in rat feces significantly decreased(P based zinc nanoparticles have the potential to act as antibiotic agents.

关键词： Aerobic bacteria Antibiotics Coliforms Nanomaterials Oxidative stress

C60 Fullerene Derivative： Radioprotective Properties and a Toxicity Test in Vivo ＆ in Witro

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材料科学与工程（中英文A版） 2012年第3期2卷 302-312页

作者： Eva Zemanova pavel Danihelka Karel Klouda Karel Zeman Faculty of Safety Engineering VSB Technical University of Ostrava Lumirova 13/630 Ostrava 700 30 Czech Republic State Office for Nuclear Safety Senovazne namesti 9 11000 Praha 1 Czech Republic

biodegradable Protection for Medical Devices with Medical Drugs Controlled Separation

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Journal of Pharmacy and Pharmacology 2016年第5期4卷 226-230页

作者： Milentina V. Seregina Evgeny A.Nemets Alina A. Akhmedova pavel b. Kurapov Elena Yu. bachtenko Laboratory of Tissue Engineering and Delivery Systems Shumakov Federal Research Center of Transplantology and ArtificialOrgans Schukinskaya street 1 Moscow 117997 Russia Department of Medical Nanobiotechnologies Pirogov Russian State Medical University Moscow 117997 Russia Vologda State University Vologda 160000 Russia

With the aim of creating biodegradable materials for medical devices clinical appointments with high hemocompatibility we have developed a new polymer *** basis of this product is plasticized by polyethylene glycol bacterial copolymer of hydroxybutyrate and oxovalerate. A well-known antitbrombotic supplement--acetylsalicylic acid has been added to improve hemocompatibility in the polymer. The results of our studies showed a controlled prolonged separation of acetylsalicylic acid from polymeric material in the blood. We studied in vitro the dynamics of liberation of acetylsalicylic acid from polymeric coatings. It was shown that the concentration of polyethylene glycol and the thickness of the polymer layer can affect the rate of diffusion of acetylsalicylic acid from polymer films.

关键词： bacterial biodegradable copolymers poly (hydroxybutyrate-co-oxovalemte) hemocompatible medical devices forclinical application polyethylene glycol acetylsalicylic acid.

Photoluminescence control by hyperbolic metamaterials and metasurfaces:a review

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Opto-Electronic Advances 2021年第8期4卷 30-56页

作者： Leonid Yu.beliaev Osamu Takayama pavel N.Melentiev Andrei V.Lavrinenko DTU Fotonik-Department of Photonics Engineering Technical University of Denmark0rsteds Plads 343DK-2800 Kgs.LyngbyDenmark Institute of Spectroscopy RAS Moscow 108840Russia Higher School of Economics National Research UniversityMoscow 101000Russia

Photolu min esce nee in clud ing fluoresce nee plays a great role in a wide variety of applicati ons from biomedical sensing and imag ing to optoelectr on ***,the enhan ceme nt and con trol of photolu min esce nee has imme nse impact on both fun dame ntal scie ntific research and aforeme nti oned applicati *** various nano phot tonic schemes and nanostructures to enhance the photoluminescence,we focus on a certain type of nanostructures,hyperbolic metamaterials(HMMs).HMMs are highly ani sotropic metamaterials,which produce intense localized electric ***,HMMs n aturally boost photolu min esce nee from dye molecules,qua ntum dots,n itroge n-vaca ncy cen ters in diam on ds,perovskites and tra nsiti on metal dichalcoge *** provide an overview of various con figuratio ns of HMMs,i nclud ing metal-dielectric multilayers,trenches,metallic nanowires,and cavity structures fabricated with the use of noble metals,transparent conductive oxides,and refractory metals as plasmonic *** also discuss lasing action realized with HMMs.

关键词： fluorescence metamaterials metasurfaces Purcell effect nanophotonics hyperbolic metamaterials

Antibody-Like Phosphorylation Sites in Focus of Statistically based bilingual Approach

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Computational Molecular bioscience 2016年第1期6卷 1-22页

作者： Jaroslav Kubrycht Karel Sigler pavel Souček Jiří Hudeček Department of Physiology Second Faculty of Medicine Charles University Prague Czech Republic Laboratory of Cellular biology Institute of Microbiology Academy of Sciences of the Czech Republic Prague Czech Republic Toxicogenomics Unit National Institute of Public Health Prague Czech Republic Department of biochemistry Charles University Prague Czech Republic

In accordance with previous reports, the sequences related to phosporylated protein segments occur in conserved variable domains of immunoglobulins including first of all certain N-terminally located segments. Consequently, we look here for the sequences 1) composing human and mouse proteins different from antigen receptors, 2) identical with or highly similar to nucleotide sequence representatives of conserved variable immunoglobulin segments and 3) identical with or closely related to phosphorylation sites. More precisely, we searched for the corresponding actual pairs of DNA and protein sequence segments using five-step bilingual approach employing among others a) different types of bLAST searches, b) two in-principle-different machine-learning methods predicting phosphorylated sites and c) two large databases recording existing phosphorylation sites. The approach identified seven existing phosphorylation sites and thirty-seven related human and mouse segments achieving limits for several predictions or phylogenic parameters. Mostly serines phosporylated with ataxia-telangiectasia-related kinase (involved in regulation of DNA-double-strand-break repair) were indicated or predicted in this study. Hypermutation motifs, located in effective positions of the selected sequence segments, occurred significantly less frequently in transcribed than non-transcribed DNA strands suggesting thus the incidence of mutation events. In addition, marked differences between the numbers and proportions of human and mouse cancer-related sequence items were found in different steps of selection process. The possible role of hypermutation changes within the selected segments and the observed structural relationships are discussed here with respect to DNA damage, carcinogenesis, cancer vaccination, ageing and evolution. Taken together, our data represent additional and sometimes perhaps complementary information to the existing databases of empirically proven phosphorylation sites or pa

关键词： Ataxia Telangiectasia-Mutated-Protein (i.e. Kinase ATM Whose Pathogenic Mutation Is Responsible for Early Death of People) Complementarity Determining Region 1 (of Immunoglobulins i.e. CDR1 or Hypervariable Region 1) Database (of Functional Structures) Hypermutation (i.e. Mutation of DNA Sequences Mediated by Enzymes) Immunoglobulin (i.e. Ig or Antibody) Phosphorylation (Enzyme Mediated Modification Concerns Here Mostly Protein Sequences)

Different clinical presentations of metachronous pulmonary metastases after resection of pancreatic ductal adenocarcinoma: Retrospective study and review of the literature

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World Journal of Gastroenterology 2017年第35期23卷 6420-6428页

作者： Martin Lovecek pavel Skalicky Josef Chudacek Marek Szkorupa Hana Svebisova Radmila Lemstrova Jiri Ehrmann bohuslav Melichar Tharani Yogeswara Dusan Klos Radek Vrba Roman Havlik beatrice Mohelnikova-Duchonova Department of Surgery I University Hospital Olomouc and Faculty of Medicine and DentistryPalacky University Department of Oncology University Hospital Olomouc and Faculty of Medicine and DentistryPalacky University Department of Molecular Pathology Faculty of Medicine and DentistryPalacky University OlomoucUniversity Hospital Olomouc Institute of Molecular and Translational Medicine Faculty of Medicine and DentistryPalacky University Department of Oncology Faculty of Medicine and DentistryPalacky University OlomoucUniversity Hospital Olomouc Department of Surgery I Faculty of Medicine and DentistryPalacky University

AIM To analyze pancreatic cancer patients who developed metachronous pulmonary metastases(MPM) as a first site of recurrence after the curative-intent *** One-hundred-fifty-nine consecutive pancreatic ductal adenocarcinoma(PDAC) patients who underwent radical pancreatic surgery between 2006 and 2013 were included in this retrospective analysis. The clinical data including age, sex, grade, primary tumor location, p TNM stage, lymph node infiltration, microangioinvasion, perineural invasion, lymphovascular invasion, the therapy administered, and follow-up were all obtained from medical records. Further analysis covered only patients with metachronous metastases. Clinical and histopathological data(age, sex, grade, primary tumor location, p TNM stage, lymph node infiltration, microangioinvasion, perineural invasion, lymphovascular invasion, the therapy administered and follow-up) of patients with metachronous non-pulmonary metastases and patients with metachronous pulmonary metastases were statistically assessed. Disease-free survival(DFS) from pancreas resection until metastases onset and overall survival(OS) were calculated. Wilcoxon test, χ~2 test and survival functions computed by the KaplanMeier method were used. Statistical significance was evaluated by the log-rank test using SPSS. A P-value of less than 0.05 was considered statistically *** Metachronous pulmonary metastases were observed in 20(16.9%) and were operable in 3(2.5%) of PDAC patients after a prior curative-intent surgery. Patients with isolated pulmonary metastases(oligometastases and multiple metastases) had estimated prior DFS and OS of 35.4 and 81.4 mo, respectively, and those with metachronous pulmonary metastases accompanied by other metastases had prior DFS and OS of 17.3 and 23.4 mo, respectively. Patients with non-pulmonary metastases had prior DFS and OS of 9.4 and 15.8 mo, respectively. Different clinical scenarios according to the presentation of MPM were obser

关键词： Pancreatic cancer Pulmonary metastases Metachronous metastases Surgical resection Overall survival Chemotherapy

Integrated binary hologram to monitor cargo release from a drug-eluting film

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Light(Advanced Manufacturing) 2022年第3期3卷 93-102页

作者： Arkady S.Abdurashitov pavel IProshin Valery V.Tuchin Gleb b.Sukhorukov Center for Neurobiology and brain Restoration Skolkovo Institute of Science and TechnologyMoscow 121205Russia Science Medical Center Saratov State UniversitySaratov 410012Russia A.N.bach Institute of biochemistry Research Center of Biotechnology of the Russian Academy of SciencesMoscow119071Russia School of Engineering and Materials Science Queen Mary University of LondonLondon E14NSUnited Kingdom Siberian State Medical University Moskovskiy Trakt2Tomsk 634050Russia

Nowadays,holography translates from a pure technical tool for recording the phase and amplitude of the light wave to a widely applicable research-based *** devices are used for security enhancement,entertainment,3D display technologies and augmented *** computer-generated holograms(amplitude or phase-based)are of specific *** are easy to compute and their manufacturing methods are fast and *** this work,a method of manufacturing amplitude-only binary holograms out of biopolymers films is *** cargo bits made out of different bioactive substances(antibiotics,dyes,etc.)absorb or scatter specific parts of the incoming light *** release was conducted by submerging the produced holograms into the aqueous solution at specific pH and temperature *** elution from the film was traced out by the numerical analysis of the far-field diffraction pattern,which otherwise could only be measured post-factum using sophisticated spectroscopic or chromatography *** approach broadens the application of general holography over to the field of biomedical research relevant to quantitative monitoring of the drug elution.

关键词： Computer generated holograms biopolymers Release monitoring Payload holograms

离子色谱电导法检测白酒、红酒、啤酒和多种黄酒中的6种阳离子

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食品科学 2020年第6期41卷 310-315页

作者：茅嘉田周晚晴 pavel NNESTERENKO 叶明立谢广发陈梅兰浙江树人大学生物与环境工程学院浙江杭州310028 莫斯科国立莱蒙诺索夫大学物理化学部化学系俄罗斯莫斯科119991

为探讨不同的酿造原料、发酵程度(乙醇体积分数)、酿造方式及酒龄等因素对黄酒中阳离子浓度水平的影响,特别是黄酒中的NH4+含量。采用离子色谱抑制电导法检测并对比黄酒、白酒、啤酒、葡萄酒4种酒样类型(共27个样品)中阳离子含量变化。... 详细信息

为探讨不同的酿造原料、发酵程度(乙醇体积分数)、酿造方式及酒龄等因素对黄酒中阳离子浓度水平的影响,特别是黄酒中的NH4+含量。采用离子色谱抑制电导法检测并对比黄酒、白酒、啤酒、葡萄酒4种酒样类型(共27个样品)中阳离子含量变化。色谱分离选用Ion Pac CS16分析柱(5 mm×250 mm)和Ion Pac CG16保护柱(5 mm×50 mm),淋洗液为30 mmol/L甲基磺酸溶液。结果表明:方法回归系数良好(r2≥0.9990),相对标准偏差不大于5.52(n=6),在检测的18种黄酒中,NH4+的质量浓度范围为137.00~284.01 mg/L,在啤酒及葡萄酒中为28.29~42.88 mg/L,在白酒中未检出。进一步研究表明,黄酒中高含量NH4+与原料中粗蛋白含量有关,NH4+的含量与发酵程度有关;其次黄酒中阳离子的含量与酒龄没有明显规律。作为蒸馏酒的白酒中整体阳离子含量普遍较低,葡萄酒由于葡萄原料的K+含量较高,导致葡萄酒中K+含量远高于其他类型酒样。

关键词：阳离子黄酒碱金属阳离子镁钙铵发酵酿造

In search of effective therapies to overcome resistance to Temozolomide in brain tumours

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Cancer Drug Resistance 2019年第4期2卷 1018-1031页

作者： Kaouthar bouzinab Helen Summers Jihong Zhang Malcolm F.G.Stevens Christopher J.Moody Lyudmila Turyanska Neil R.Thomas pavel Gershkovich Marianne b.Ashford Emily Vitterso Lisa C.D.Storer Richard Grundy Tracey D.bradshaw School of Pharmacy University of NottinghamNottingham NG72RDUK School of Chemistry University of NottinghamNottingham NG72RDUK School of Physics and Astronomy University of NottinghamNottingham NG72RDUK Advanced Drug Delivery Pharmaceutical SciencesR&DAstraZenecaMacclesfield SK104TFUK School of Medicine Queen’s Medical CentreUniversity of NottinghamNottingham NG72UHUK.

Glioblastoma multiforme is the most common and lethal brain *** current standard of care includes Temozolomide(TMZ)***,inherent and acquired resistance to TMZ thwart successful *** direct repair protein methylguanine DNA methyltransferase(MGMT)removes the cytotoxic O6-methylguanine(O6-MeG)lesion delivered by TMZ and so its expression by tumours confers *** mismatch repair(MMR)is essential to process O6-MeG adducts and MMR-deficiency leads to tolerance of lesions,resistance to TMZ and further DNA *** this article,two strategies to overcome TMZ resistance are discussed:(1)synthesis of imidazotetrazine analogues-designed to retain activity in the presence of MGMT or loss of MMR;(2)preparation of imidazotetrazine-nanoparticles to deliver TMZ preferably to the brain and tumour *** promising results encourage belief in a future where better prognoses exist for patients diagnosed with this devastating disease.

关键词： brain cancer temozolomide-resistance analogues drug delivery apoferritin