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TNF system in eutopic endometrium from women with endometriosis

Open Journal of Obstetrics and Gynecology 2013年第2期3卷 271-278页

作者： María Angélica Boric Marisa Torres Claudio Pinto Maricela Pino paulina Hidalgo Fernando Gabler Ariel Fuentes María Cecilia Johnson Institute of Maternal and Child Research Faculty of Medicine University of Chile Santiago Chile

The role of the tumor necrosis factor (TNF) system in endometriosis is not completely clear and therefore was the focus of this study. In eutopic endometria obtained from women with (n = 41) and without (controls;n = 34) endometriosis during laparoscopy, the subcellular location and the percentages for TNF, TNFR1, TNFR2 and CD45 positive-cells were determined (immunohistochemistry);the protein concentration (ELISA) of the soluble receptors (sTNFR1 and sTNFR2) were measured in 4h-explants culture media and the TNF concentration was performed in ex vivo endometrial homogenates. The TNF, TNFR1 and TNFR2 mRNAs were analyzed by real-time PCR. In control endometria, TNF, TNFR1 and sTNFR1 proteins increased during the late secretory phase. In endometriosis endometria, the protein highest level of TNFR1 was reached during the early and the mid secretory phases and of sTNFR1 concentration during the proliferative phase;however, during the late secretory phase, both TNFR1 and sTNFR1 protein levels were reduced compared to the control endometria (p sTNFR2 concentrations, and TNF and TNFR2 mRNAs were similar in control and endometriosis endometria. Although TNFR1 mRNA was highly expressed, no significant differences were found between control and endometriosis endometria. In summary, this study establishes that TNF and TNFR1 protein expressions and the sTNFR concentrations in eutopic endometria from women with and without endometriosis are dependent on the menstrual cycle. The differences on the TNFR1 and sTNFR1 protein pattern between both groups, mainly during the mid and late secretory phases may play a role in the lower implantation rates observed in women with endometriosis, and also could be related to the altered cell death, which favor the augmented cell viability facilitating the characteristic endometrial implantation and growth outside the uterus in this disease.

关键词： Endometriosis Eutopic Endometrium TNF sTNFR1 sTNFR2

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Polymer Microtips Fabricated at the Extremity of Photonic Crystal Fibers

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材料科学与工程（中英文B版） 2013年第12期3卷 749-758页

作者： paulina Pura Michat Szymanski Bartosz Wisniowski Michal Dudek Leszek R. Jaroszewicz MarekNapierata Anna Spadto Pawel Marc Malgorzata Kujawinska Faculty of New Technologies and Chemistry Military University of Technology Warsaw 00-908 Poland Institute of Micromechanics and Photonics Warsaw University of Technology Warsaw 02-525 Poland InPhoTech Ltd. Warsaw 00-195 Poland

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脐带间充质干细胞在细胞治疗中的应用潜能

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长春中医药大学学报 2012年第3期28卷 519-521页

作者：褚晓倩王丽 paulina Duya 边育红天津中医药大学天津300193 天津市传染病医院天津300192

人脐带间充质干细胞是近年人们从胎儿脐带华尔通氏胶部位分离得到的基质细胞,具有一定干细胞特性,已证实有多向分化潜能和可塑性。脐带间充质干细胞来源丰富,培养方法简单,增殖能力旺盛,排异反应低,无伦理道德争议,可望成为自体与同种... 详细信息

人脐带间充质干细胞是近年人们从胎儿脐带华尔通氏胶部位分离得到的基质细胞,具有一定干细胞特性,已证实有多向分化潜能和可塑性。脐带间充质干细胞来源丰富,培养方法简单,增殖能力旺盛,排异反应低,无伦理道德争议,可望成为自体与同种异体移植及细胞替代治疗的理想细胞来源。

关键词：脐带间充质干细胞增殖能力排异反应多能性细胞治疗

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关于迈阿密使用FRP材料修复混凝土管的讨论

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非开挖技术 2016年第4期 53-58页

作者： Jonathan Wise paulina Wasco Scott Arnold 沙伟奇

玻璃钢（FPR）材料具有重量轻的特点，由饱和碳纤维聚合物基体组成。当这种材料被固化后会形成具有强度的构件，这可以用来加固现有的给排水管道基础设施。FPR材料能够在短时间内固化并达到工作所需条件和强度，因此，近来被提出用于修... 详细信息

玻璃钢（FPR）材料具有重量轻的特点，由饱和碳纤维聚合物基体组成。当这种材料被固化后会形成具有强度的构件，这可以用来加固现有的给排水管道基础设施。FPR材料能够在短时间内固化并达到工作所需条件和强度，因此，近来被提出用于修复管道及接头；同样也适用于损毁管线的紧急修复和铺设。最近，迈阿密戴德县给排水公司（MDWASD）检查了他们管辖的一段长129英里的预应力钢筒混凝土（PCCP）管道，发现大量的压力管道因腐蚀而被损坏。本文旨在此背景下讨论使用FRP材料修复WASD的54英寸PCCP管线，采用的是美国水工程协会（AWWA）M45玻璃纤维管的设计标准。

关键词： FPR复合材料 PCCP管管道修复

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Nature products of traditional Chinese medicine provide new ideas in γδT cells for tumor immunotherapy

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Acupuncture and Herbal Medicine 2022年第2期2卷 78-83页

作者： paulina Atieno Duya Yinxiao Chen Liding Bai Zhaodong Li Jiaying Li Rundong Chai Yuhong Bian Shuwu Zhao Ministry of Health Kenya Division of Traditional and Alternative MedicineKenya Tianjin University of Traditional Chinese Medicine Tianjin 301617China

Due to the unique features of innate immune cells, the role of γδT cells in tumor immunity has gradually attracted more and more attention. Previous studies have found that γδT cells play a dual role in tumor immunology: tumor-promoting and *** anti-tumor therapy of γδT cells has made remarkable success in clinical application. Especially in recent years, researchers have provided some novel effective ways such as γδT cells exosomes and adoptive chimeric antigen receptor-γδT cells immunotherapy. However, some problems remain to be solved, such as low expansion rate, poor targeting, and tumor microenvironment limiting the effectiveness of γδT immunotherapy. Traditional Chinese medicine is expected to play a positive role in the body immune-enhancing function, promoting the proliferation and activation of γδT cells, and inducing the differentiation ofγδT cells. In this review, we summarize the recent research progress and urgent problems of γδT cells in anti-tumor immunotherapy. Moreover, some new strategies of γδT cells for tumor immunotherapy were proposed.

关键词： γδT cells Traditional Chinese medicine Tumor immunotherapy

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Effect of Photon Flux Density and Exogenous Sucrose on the Photosynthetic Performance during In Vitro Culture of Castanea sativa

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American Journal of Plant Sciences 2016年第14期7卷 2087-2105页

作者： Patricia L. Sáez León A. Bravo Manuel Sánchez-Olate paulina B. Bravo Darcy G. Ríos Laboratorio Cultivo de Tejidos Vegetales Facultad de Ciencias Forestales y Centro de Biotecnología Universidad de Concepción Concepción Chile Laboratorio de Fisiología y Biología Molecular Vegetal Instituto de Agroindustria Departamento de Ciencias Agronómicas y Recursos Naturales Fac. de Cs Agropecuarias y Forestales & Center of Plant Soil Interaction and Natural Resources Biotechnology Scientific and Technological Bioresource Nucleus Universidad de La Frontera Temuco Chile Center of Plant Soil Interaction and Natural Resources Biotechnology Scientific and Technological Bioresource Nucleus Universidad de La Frontera Temuco Chile

The low photon flux density (PFD) under in vitro conditions and sucrose added to the culture medium negatively limits the photochemical activity and photoprotective mechanisms of microshoots. In this work we hypothesize that decreasing sucrose in the culture medium in combination with increasing irradiance, could improve the photosynthesis and consequently the in vitro growth. We evaluated the effect of exogenous sucrose (30 and 5 g·L-1, HS and LS, respectively), under different PFD (50 and 150 μmol photons m-2·s-1, LL and HL, respectively) on the photosynthetic performance and growth of Castanea sativa microshoots. Decreasing sucrose negatively affected the physiological attributes evaluated. Only chloroplast ultrastructure was improved by LS;however this did not lead to an improved in photosynthesis or growth. HL HS produced an increase in photosynthetic activity and chlorophyll contents, reaching under these conditions a higher proliferation rate and biomass production. Additionally, the photochemical activity (electron transport rate and non-photochemical quenching) was improved by HL. Thus, our results suggest that, at least for C. sativa HL is beneficial during the in vitro culture, improving photosynthetic performance as well as growth, but this is only possible in the presence of moderate concentrations of sucrose added to the culture medium.

关键词： Sucrose PFD Micropropagation Photosynthesis Chlorophyll Fluorescence

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加密圈的至暗时刻投资者损失惨重

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商业周刊（中文版） 2022年第21期 24-25页

作者： Claire Ballentine paulina Cachero Jeremy Hill 王忠(译) 不详

有段时间,陷入困境的FTX的部分美国客户似乎可以避免该加密货币平台所面临的最坏情况。FTXUS是萨姆·班克曼-弗里德(Sam Bankman-Fried)***平台旗下的独立实体。该公司11月10日在推特上表示,尽管整个集团的其他部门相继倒下,但该公司仍... 详细信息

有段时间,陷入困境的FTX的部分美国客户似乎可以避免该加密货币平台所面临的最坏情况。FTXUS是萨姆·班克曼-弗里德(Sam Bankman-Fried)***平台旗下的独立实体。该公司11月10日在推特上表示,尽管整个集团的其他部门相继倒下,但该公司仍正常运营,提款也在正常受理。但在班克曼一弗里德的金融帝国于11月11日申请破产保护后,FTXUS的客户就迅速陷入了一场让整个行业感到不安。

关键词：弗里德金融帝国最坏情况客户投资者

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滇池中溶藻细菌的分离鉴定及其溶藻效应

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微生物学通报 2018年第12期45卷 2614-2623页

作者：王琪 paulina Simon 刘锦钰迟玉新代先祝杜红霞张晓辉五十岚泰夫杨彩云罗锋西南大学资源环境学院重庆400715

【背景】藻类水华或赤潮在世界范围内频发,带来各种危害,亟需找到有效途径控制水华或赤潮。溶藻细菌具有杀死藻类控制藻类生物量的能力,可以作为防治水华和赤潮的有效工具。【目的】分离并鉴定滇池中的铜绿微囊藻(Microcystisaeruginosa... 详细信息

【背景】藻类水华或赤潮在世界范围内频发,带来各种危害,亟需找到有效途径控制水华或赤潮。溶藻细菌具有杀死藻类控制藻类生物量的能力,可以作为防治水华和赤潮的有效工具。【目的】分离并鉴定滇池中的铜绿微囊藻(Microcystisaeruginosa)及其溶藻细菌,对溶藻菌作用于铜绿微囊藻的溶藻效应进行研究,初步了解其溶藻特性与溶藻机制。【方法】采用LB平板稀释涂布,再经多次划线分离纯化细菌,测定16SrRNA基因序列以鉴定细菌种类;采用毛细管分离的方法分离铜绿微囊藻,并测定其cpcBA基因序列以鉴定蓝藻种类;采用热乙醇法提取叶绿素a,从而计算溶藻效率;基于过氧化氢酶(CAT)、还原型谷胱甘肽(GSH)和丙二醛(MDA)探究藻细胞在溶藻菌处理下的抗氧化系统响应。【结果】共分离获得11株微囊藻和17株针对铜绿微囊藻的高效溶藻菌。选取其中一株生长速度最快的铜绿微囊藻DCM4和一株溶藻效果最好的溶藻菌Sp37 (Bacillus siamensis)进行后续研究。Sp37对DCM4的4 d溶藻率达到92.4%±1.5%,且对微囊藻属的水华微囊藻(M. flos-aquae)和惠氏微囊藻(***)均有溶藻效果,而对绿藻没有溶藻效果。Sp37的原菌液和无菌滤液对DCM4的4d溶藻率分别为86.8%±4.3%和81.1%±2.2%,两者没有显著差异(P>0.05)。Sp37菌体对DCM4的溶藻率为25.4%±7.3%。Sp37无菌滤液经不同温度和pH处理之后的溶藻率与未经处理的无菌滤液的溶藻率无明显差异。Sp37无菌滤液处理藻细胞会使藻细胞的CAT、GSH和MDA含量发生变化。【结论】菌株Sp37对铜绿微囊藻DCM4具有高效的溶藻作用,而且对微囊藻属具有一定的溶藻特异性。Sp37是通过分泌胞外物质间接溶藻,且溶藻物质具有热稳定性和酸碱稳定性。Sp37无菌滤液处理藻细胞会触发藻细胞抗氧化系统,并且会损伤藻细胞膜。Sp37无菌滤液很可能是通过对藻细胞造成氧化胁迫,最终导致藻细胞死亡的。

关键词：溶藻菌铜绿微囊藻叶绿素a 杀藻物质抗氧化系统

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Bioequivalence Study of Two Hydrochlorothiazide Formulations after Oral Administration in Healthy Chilean Volunteers

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Journal of Pharmacy and Pharmacology 2016年第12期4卷 679-685页

作者： Fierro Humberto Rojas Romina Yanez Sebastian Acufia Paola Rosales Katherine Yevenes paulina Yzoard Marcia Tapia Claudia Rivas Violeta Caba Alvaro Copaja Miguel Jorge Fuentealba Center for Advanced Research on Biomedicine (CIAB-UdeC) University of Concepcion Concepcion 4070038 Chile Area Health and Safety AGQ Santiago 8590829 Chile Regional Hospital Guillermo Grant Benavente Concepcion 4070038 Chile Laboratory of Screening of Neuroactive Compounds Department of Physiology Faculty of Biological Sciences University ofConcepcion Concepcion 4070038 Chile

The study was carried out in healthy Chilean volunteers in order to compare the pharmacokinetics （rate and extent absorption） of two commercial oral formulations of 50 mg hydrochlorothiazide. Thirty nine subjects were administered hydrochlorothiazide tablets of test （T） and reference （R） formulation in a single blind, randomized, fasting, 2 × 2 crossover study, seven washout days. Blood samples were taken during a 48 h period after drug administration. Plasma concentrations were quantified by HPLC-MS/MS. The primary parameters log-transformed Cmax （maximum plasma concentration）, A UC0-t and A UC0-∞ （area under the plasma concentration-time curve from zero to the last time and zero to infinity） were tested for bioequivalence considering the ratios of geometric means （test/reference）; whereas tmax （the time of maximum plasma concentration） was analyzed nonparametrically. The 90% confidence intervals for the geometric mean values of test/reference ratios for Cmax, A UCo.t and A UC0-∞ were 95.37%, 93.59% and 96.34%, respectively, and were located within the bioequivalence acceptance range of 80-125%, as were tmax and elimination constants. Together, we conclude that the test formulation of the hydrochlorothiazide 50 mg tablet is bioequivalent to the reference product and suitable for generic prescription.

关键词： Bioequivalence hydrochlorothiazide interchangeable.

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Violence Towards Migrants

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Psychology Research 2018年第8期8卷 355-366页

作者： Patricia Martinez Lanz Maria Domenica Ravelo Ladron de Guevara Daniela Ayari Moreno Landeta Maria Fernanda Gen Lortia Ixchel Aditi Gonzalez Gutierrez Elsa paulina Alanis Cazares Universidad Anauac Mexico Mexico City Mexico

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