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Arabidopsis Heterotrimeric G-protein Regulates Cell Wall Defense and Resistance to Necrotrophic Fungi

Molecular Plant 2012年第1期5卷 98-114页

作者： Magdalena Delgado-Cerezo Clara Sanchez-Rodriguez Viviana Escudero Eva Miedes paula virginia fernandez Lucia Jorda Camilo Hernandez-Blanco Andrea Sanchez-Vallet Pawel Bednarek Paul Schulze-Lefert Shauna Somervilleg Jose Manuel Estevez Staffan Persson Antonio Molina Centro de Biotecnologia y Genomica de Plantas （UPM-INIA） Universidad Politecnica de Madrid Campus de Montegancedo E-28223-Pozuelo de Alarcbn （Madrid） Spain Max-Planck-lnstitute fLir Molekulare Pflanzenphysiologie Am MQhlenberg 2 14476 Potsdam Germany Laboratorio de Fisiologia y Biologia Molecular IFIByNE （CONICET） FCEyN-Universidad de Buenos Aires Pab. II Intendente Guiraldes 2160 Buenos Aires C1428EGA Argentina Present address： Laboratory of Phytopathology Wageningen University Droevendaalsesteeg 1 6708 PB Wageningen The Netherlands Max Planck Institut for Zuchtungsforschung Department of Plant Microbe Interactions Carl-von-Linne-Weg 10 D-50829 Koln Germany Institute of Bioorganic Chemistry Polish Academy of Sciences Noskowskiego 12/14 61-704 Poznan Poland Energy Biosciences Institute 130 Calvin Hall University of California at Berkeley Berkeley CA 94720 USA

The Arabidopsis heterotrimeric G-protein controls defense responses to necrotrophic and vascular fungi. The agbl mutant impaired in the Gβ subunit displays enhanced susceptibility to these pathogens. Gβ/AGB1 forms an obligate dimer with either one of the Arabidopsis Gγsubunits （γ1/AGG1 and γ2/AGG2）. Accordingly, we now demonstrate that the aggl agg2 double mutant is as susceptible as agbl plants to the necrotrophic fungus Plectosphaerella cucumerina. To elucidate the molecular basis of heterotrimeric G-protein-mediated resistance, we performed a comparative transcriptomic analysis of agbl-1 mutant and wild-type plants upon inoculation with P cucumerina. This analysis, together with metab- olomic studies, demonstrated that G-protein-mediated resistance was independent of defensive pathways required for resistance to necrotrophic fungi, such as the salicylic acid, jasmonic acid, ethylene, abscisic acid, and tryptophan-derived metabolites signaling, as these pathways were not impaired in agbl and aggl agg2 mutants. Notably, many mis-reguiated genes in agbl plants were related with cell wall functions, which was also the case in aggl agg2 mutant. Biochemical analyses and Fourier Transform InfraRed （FTIR） spectroscopy of cell walls from G-protein mutants revealed that the xylose content was lower in agbl and aggl agg2 mutants than in wild-type plants, and that mutant walls had similar FTIR spec-tratypes, which differed from that of wild-type plants. The data presented here suggest a canonical functionality of the Gβ and Gγ1/γ2 subunits in the control of Arabidopsis immune responses and the regulation of cell wall composition.

关键词： Plant immunity cell wall indole glucosinolates xylose necrotropic fungi G-protein.

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UV-B Radiation Induces Mu Element Somatic Transposition in Maize

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Molecular Plant 2013年第6期6卷 2004-2007页

作者： Julia Questa virginia Walbot paula Casati Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI) Universidad Nacional de Rosario Rosario Argentina Cell and Developmental Biology John Innes Centre Norwich Research Park UK Department of Biology Stanford University Stanford CA 94305-5020 USA

Dear Editor, The maize Mutator （MuDRIMu） transposon family is the most active DNA transposon in plants （Lisch, 2002）. The Mu family contains diverse elements, all sharing similar -215-bp terminal inverted repeats （TIRs）. MuDR is the autonomous element, and a transcriptionally active MuDR is required for transposition of the non-autonomous Mu elements （Chomet et al., 1991）. MuDR contains two genes, mudrA and mudrB （Figure 1A）; mudrA encodes the MURA transposase, and mudrB encodes a protein with unknown function. To avoid the deleterious effects of transposons, plants have acquired mechanisms to epigenetically silence them. Silenced Mu ele- ments become heavily methylated, and their reactivation is very rare. To date, only UV-B radiation treatments have reac- tivated silenced Mu （Walbot, 1999）.

关键词： UV-B 元素辐射诱导玉米换位表观遗传学转座子赋值函数

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Induction of apoptosis on human hepatocarcinoma cell lines by an alkyl resorcinol isolated from Lithraea molleoides

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World Journal of Gastroenterology 2006年第37期12卷 5959-5963页

作者： Luciana Barbini paula Lopez Julieta Ruffa virginia Martino Graciela Ferraro Rodolfo Campos Lucia Cavallaro Catedra de Virologia Facultad de Farmacia y Bioquimica Universidad de Buenos Aires. Junin 956 (1113) Buenos Aires Argentina Catedra de Farmacognosia Facultad de Farmacia y Bioquimica Universidad de Buenos Aires. Junin 956 (1113) Buenos Aires Argentina

AIM: To study the mechanism of cytotoxicity of a new active 5-alkyl resorcinol [1, 3-dihydroxy-5- (tridec-4’, 7’ -dienyl) benzene] isolated from Lithraea molleoides leaves on liver tumor cells. METHODS: Human hepatocarcinoma cell lines (HepG2 and Hep3B) in culture were treated with inhibitory concentrations, 50% of the compound, for 24 h. The induction of apoptosis was detected in treated cells by analysis of DNA fragmentation, DNA content, and acridine orange and propidium iodide staining. RESULTS: After 24 h of 5-alkyl resorcinol treatment, both cell lines showed: (1) the typical morphological alterations of apoptosis; (2) DNA fragmentation, detected by laddering and appearance of a subG0 population by flow cytometry; and (3) condensed and fragmented nuclei by acridine orange-propidium iodide staining. CONCLUSION: Based on the results, this compound exerts its cytotoxic effect in both hepatocellular cell lines through apoptotic cell death. For Hep3B, cells with mutated p53 and Fas, apoptosis would proceed by p53- or Fas-independent pathways.

关键词： Lithraea molleoides Cytotoxic activity Alkyl resorcinol Apoptosis Hepatoma

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Immune strategies of phagoctytic cells stimulated in vitro with live and heat-inactivated Streptococcus pyogenes

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BIOCELL 2013年第3期37卷 63-69页

作者： Agustín César MÖHLINGER virginia paula SIFFREDI Serenela CHULIBERT Adriana PONESSA Rodolfo David NOTARIO Diana Graciela DLUGOVITZKY Inmunology Section Microbiology DepartmentFaculty of Medical SciencesRosario National UniversityArgentina Microbiology Department Faculty of Medical SciencesRosario National UniversityArgentina

Streptococcus pyogenes(group A Streptococcus)is frequently involved in a wide range of human *** we evaluated polymorphonuclear neutrophils and mononuclear cells from healthy subjects for their bactericidal function after stimulation with live and inactivated Streptococcus pyogenes(Streptococcus Group A).Mononuclear cells and Neutrophils were isolated from heparinized blood samples(n=18)using a Ficoll-Hypaque gradient and cultured in RPMI 1640 for 18 hours with a suspension of either live or inactivated Streptococcus *** the respiratory burst(flow cytometry)and nitrite,TNF and IL17 production(ELISA)were measured in the cell culture *** increased respiratory burst(expressed as R index)was induced by both live and inactivated ***,increased nitrite,TNF and IL17 concentrations were found in cell culture supernatants in both *** findings may provide some explanation as to the roles played by neutrophils and mononuclear cells in Streptococcus pyogenes immunopathogenicity。

关键词： Respiratory burst nitrites TNFa IL17

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T_3-induced liver AMP-activated protein kinase signaling:Redox dependency and upregulation of downstream targets

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World Journal of Gastroenterology 2014年第46期20卷 17416-17425页

作者： Luis A Videla virginia Fernández Pamela Cornejo Romina Vargas paula Morales Juan Ceballo Alvaro Fischer Nicolás Escudero Oscar Escobar Molecular and Clinical Pharmacology Program Institute of Biomedical SciencesFaculty of MedicineUniversity of ChileIndependencia 1027SantiagoChile School of Medical Technology Faculty of MedicineDiego Portales UniversityEjército 141SantiagoChile

AIM: To investigate the redox dependency and promotion of downstream targets in thyroid hormone (T3)-induced AMP-activated protein kinase (AMPK) signaling as cellular energy sensor to limit metabolic stresses in the l... 详细信息

关键词： Liver Thyroid hormone N-acetylcysteine AMP-activated protein kinase Fatty acid oxidation

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Baseline Susceptibilities of B-and Q-Biotype Bemisia tabaci to Chlorantraniliprole

Baseline Susceptibilities of B-and Q-Biotype Bemisia tabaci ...

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The 5th International Bemisia Workshop

作者： Benjamin Degain virginia Harpold paula Marcon Robert L.Nichols Department of Entomology and BIO5 institute University of ArizonaAZ 85721USA.

Susceptibility to chlorantraniliprole(Rynaxypyr),an anthranilic diamide insecticide,was determined for three laboratory strains(one pyriproxyfen-susceptible B biotype strain,one pypriproxyfen-resistant B biotype strain,and one multiple resistant Q biotype strain) and seven field populations of the B biotype sweetpotato whitefly(Bemisia tabaci Gennadius.

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发展园艺类治疗方法理论的重要概念

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风景园林 2024年第5期31卷 54-74页

作者：保拉·黛安娜·莱尔夫薛滨夏(译) 路森婷(译) 弗吉尼亚理工大学植物与环境科学、农业与生命科学学院美国园艺疗法协会国际人与植物理事会美国园艺科学学会哈尔滨工业大学建筑与设计学院哈尔滨工业大学寒地城乡人居环境科学与技术工业和信息化部重点实验室

【目的】为厘清园艺疗法的概念界定,深入思考其理论发展的重要问题。【方法/过程】回顾并总结园艺疗法在自然环境与人类健康相关学科领域的发展过程中需要解决的关键问题。基于50多年来在全球范围内开展的与园艺疗法相关的教育、实践、... 详细信息

【目的】为厘清园艺疗法的概念界定,深入思考其理论发展的重要问题。【方法/过程】回顾并总结园艺疗法在自然环境与人类健康相关学科领域的发展过程中需要解决的关键问题。基于50多年来在全球范围内开展的与园艺疗法相关的教育、实践、研究、教学、合作、写作和咨询活动,结合当前大量的文献综述,鼓励读者通力合作,共同解决对园艺疗法领域的发展至关重要的问题。【结果/结论】为读者提供了多样的方法来理解和解决园艺疗法作为理论、模型和应用发展一部分的关键问题。

关键词：自然辅助疗法园艺疗法治疗性园艺分类理论关键概念

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