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Cell-matrix reciprocity in 3D culture models with nonlinear elasticity

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Bioactive Materials 2022年第3期7卷 316-331页

作者： Kaizheng Liu Maury Wiendels Hongbo Yuan Changshun Ruan paul H.j.Kouwer Research Center for Human Tissue and Organs Degeneration Institute of Biomedicine and BiotechnologyShenzhen Institutes of Advanced TechnologyChinese Academy of SciencesShenzhen518055PR China Radboud University Institute for Molecules and MaterialsHeyendaalseweg 1356525 AJNijmegenthe Netherlands Institute of Biophysics Hebei University of TechnologyTianjin300401PR China Molecular Imaging and Photonics Chemistry DepartmentKU LeuvenCelestijnenlaan 200F3001HeverleeBelgium

Three-dimensional(3D)matrix models using hydrogels are powerful tools to understand and predict cell *** interactions between the cell and its matrix,however is highly complex:the matrix has a profound effect on basic cell functions but simultaneously,cells are able to actively manipulate the matrix ***(mechano)reciprocity between cells and the extracellular matrix(ECM)is central in regulating tissue functions and it is fundamentally important to broadly consider the biomechanical properties of the in vivo ECM when designing in vitro matrix *** manuscript discusses two commonly used biopolymer networks,*** and fibrin gels,and one synthetic polymer network,polyisocyanide gel(PIC),which all possess the characteristic nonlinear mechanics in the biological stress *** start from the structure of the materials,then address the uses,advantages,and limitations of each material,to provide a guideline for tissue engineers and biophysicists in utilizing current materials and also designing new materials for 3D cell culture purposes.

关键词： Artificial extracellular matrices Mechanical reciprocity Collagen Fibrin Polyisocyanides

An algorithm for family screening for coeliac disease

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World journal of Gastroenterology 2006年第48期12卷 7805-7809页

作者： jocelyn S Fraser Alistair L King H julia Ellis Simon j Moodie Ingvar Bjarnason jill Swift paul j Ciclitira Division of Nutritional Sciences King's College London United Kingdom Division of Nutritional Sciences King’s College London United Kingdom

AIM: To assess the level of undiagnosed coeliac disease (CD) in relatives of patients affected by the condition. METHODS: We collected blood from 914 relatives of probands. We screened these individuals by ELISA for IgA and IgG tTG antibodies, confirming any positive IgA tTG results with an IgA EMA and looked for evidence of IgA deficiency in those who were IgG tTG positive alone, and performed IgG1 EMA in these individuals. We undertook HLA typing where positive screening was found, and this confirmed a strong prevalence of HLA- DQ2 in the coeliac population. Follow-up small intestinal biopsy was undertaken in cases with positive serological screening, wherever possible. RESULTS:Use of this serological screening algorithm revealed a prevalence of undiagnosed CD in 5%-6% of first degree relatives of probands. CONCLUSION:Our data suggests that first degree relatives of individuals with CD should be screened for this condition.

关键词： Coeliac disease Screening Endomysialantibody Familial study IgA deficiency Prevalence Tissue transglutaminase

Feasibility study of phase-sensitive imaging based on multiple reference optical coherence tomography

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Chinese Optics Letters 2017年第9期15卷 25-30页

作者： Roshan Dsouza Hrebesh Subhash Kai Neuhaus paul M.McNamara josh Hogan Carol Wilson Martin j.Leahy Tissue Optics and Microcirculation Imaging Group School of PhysicsNational University of IrelandGalway H91 CF50Ireland Compact Imaging Ireland Ltd. Galway H91 CF50Ireland Colgate-Palmolive Global Technology Centre Piscataway NJ 08855USA Compact Imaging Inc.Mountain View 94043USA Royal College of Surgeons(RCSI) DublinIreland

Multiple reference optical coherence tomography（MR-OCT） is a recently developed, low-cost and compact time-domain OCT solution for primary care and consumer level applications. A combination of a voice coil actuator and a partial mirror（PM） extends the scan range for imaging depths of approximately 1 mm in biological samples. Our previous research on MR-OCT is based only on intensity information obtained from the depth-resolved interference signal. In this Letter, we extract the phase information from the MR-OCT signal and, hence, provide an additional contrast modality. The phase sensitivity of the system is measured to be approximately 0.2 and 1.5 rad for the first and twelfth orders of reflection when using a mirror as the *** Letter describes first results of phase-sensitive data measured on a phantom obtained with MR-OCT. Data from a chick embryo chorioallantoic membrane（CAM） is used to demonstrate the feasibility of MR-OCT for in vivo phase-sensitive imaging.

关键词： Feasibility study of phase-sensitive imaging based on multiple reference optical coherence tomography VCM Figure CAM

Genotypes and viral variants in chronic hepatitis B: A review of epidemiology and clinical relevance

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World journal of Hepatology 2015年第3期7卷 289-303页

作者： Catherine MN Croagh paul V Desmond Sally j Bell Department of Gastroenterology St Vincent's Hospital

The Hepatitis B Virus(HBV) has a worldwide distribu-tion and is endemic in many populations. It is constantly evolving and 10 genotypic strains have been identified with varying prevalences in different geographic regions. Numerous stable mutations in the core gene and in the su-rface gene of the HBV have also been identified in untreated HBV populations. The genotypes and viral variants have been associated with certain clinical featu-res of HBV related liver disease and Hepatocellu-lar carcinoma. For example Genotype C is associated withlater hepatitis B e antigen(HBe Ag) seroconversion, and more advanced liver disease. Genotype A is associated with a greater risk of progression to chronicity in adu-lt acqu-ired HBV infections. Genotype D is particu-larly associated with the precore mu-tation and HBe Ag negative chronic hepatitis B(CHB). The genotypes prevalent in parts of West Africa, Central and Sou-th America, E, F and H respectively, are less well stu-died. Viral variants especially the Basal Core Promotor mutation is associated with increased risk of fibrosis and cancer of the liver. Althou-gh not cu-rrently part of rou-tine clinical care, evalu-ation of genotype and viral variants may provide u-sefu-l adju-nctive information in predicting risk abou-t liver related morbidity in patients with CHB.

关键词： Chronic hepatitis B Genotype Pre-core Basal core promotor Mutations

All-optical tuning of a diamond micro-disk resonator on silicon

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Photonics Research 2020年第3期8卷 318-324页

作者： paul HILL CHARALAMBOS KLITIS BENOIT GUILHABERT MARC SOREL ERDAN GU MARTIN D.DAWSON MICHAEL j.STRAIN Institute of Photonics Department of PhysicsTechnology and Innovation CentreUniversity of StrathclydeGlasgow G11RDUK Diamond Science and Technology Centre for Doctoral TrainingUniversity of WarwickCoventry CV47ALUK School of Engineering University of GlasgowGlasgow G128LTUK

High-quality integrated diamond photonic devices have previously been demonstrated in applications from nonlinear photonics to on-chip quantum ***,the small sample sizes of single crystal material available,and the difficulty in tuning its optical properties,are barriers to the scaling of these *** of these issues can be addressed by integrating micrometer-scale diamond devices onto host photonic integrated circuits using a highly accurate micro-assembly *** this work a diamond micro-disk resonator is integrated with a standard single-mode silicon-on-insulator waveguide,exhibiting an average loaded Q-factor of 3.1×10^4 across a range of spatial modes,with a maximum loaded Q-factor of 1.05×10^*** micrometer-scale device size and high thermal impedance of the silica interface layer allow for significant thermal loading and continuous resonant wavelength tuning across a 450 pm range using a milliwatt-level optical *** diamond-on-demand integration technique paves the way for tunable devices coupled across large-scale photonic circuits.

关键词： tuning resonator waveguide

Effect of Operational Changes in Reducing Fish Impingement at a Power Plant in Ohio, USA

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journal of Environmental Science and Engineering(A) 2015年第2期4卷 55-66页

作者： paul Henry Patrick Elaine Mason Darlene Ager scott Brown ARCADIS Canada Inc. (formerly ARCADIS SENES Canada Inc.) Richmond Hill L4B3N4 Ontario Canada Kinectrics North America Inc. Toronto M8Z5G5 Ontario Canada FirstEnergy Corp. Akron 44308 Ohio USA

Both intake volumetric flow and through-screen velocity （the velocity of water as it passes through the screen） are important variables affecting fish impingement at industrial water intake structures including those at power plants. However, there are limited data available on quantitative assessments of impingement following changes in power plant operation such as reduced volumetric flow and intake velocity. Impingement studies were conducted at Bay Shore Power Plant in 2005-2006 （baseline） and again in 2013-2014 following fish protection mitigation which included reduced intake volumetric flows （from 33.5 m^3·s^-1 to 9.1 cm·s^-1）, a reduced through-screen velocity （from approximately 79.2 cm·s^-1 to 11.6 cm·s^-1）, modified traveling screens and installation of a fish-return system to gently and quickly remove any fish that were impinged back into the waterbody. A comparison of baseline and post-mitigation results suggested that with this mitigation in place, impingement reductions can exceed 90% for robust dominant fish species in the area.

关键词： Impingement fish protection cooling water intake structure flow and velocity reduction mitigation.

Photocatalytic reduction of organic pollutant under visible light by green route synthesized gold nanoparticles

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journal of Environmental Sciences 2017年第5期29卷 236-246页

作者： Bharat C.Choudhary Debajyoti paul Tarun Gupta Sandesh R.Tetgure Vaman j.Garole Amulrao U.Borse Dipak j.Garole School of Chemical Sciences North Maharashtra University Jalgaon 425001 Maharashtra India Centre for Environmental Science & Engineering Indian Institute of Technology Kanpur Kanpur 208016 Uttar Pradesh India Department of Earth Sciences Indian Institute of Technology Kanpur Kanpur 208016 Uttar Pradesh India Enoironmental Enoineering and Manaoement Department of Civil Engineering Indian Institute of Technology Kanpur Kanpur 208016 Uttar Pradesh India Directorate of Geology and Mining Government of Maharashtra Nagpur 440010 Maharashtra India

We report a rapid method of green chemistry approach for synthesis of gold nanoparticles（AuNPs）using Lagerstroemia speciosa leaf extract（LSE）. L. speciosa plant extract is known for its effective treatment of diabetes and kidney related problems. The green synthesis of Au NPs was complete within 30 min at 25°C. The same could also be achieved within 2 min at a higher reaction temperature（80°C）. Both UV–visible spectroscopy and transmission electron microscopy results suggest that the morphology and size distribution of Au NPs are dependent on the pH of gold solution,gold concentration,volume of LSE,and reaction time and temperature. Comparison between Fourier transform infrared spectroscopy（FT-IR）spectra of LSE and the synthesized Au NPs indicate an active role of polyphenolic functional groups（from gallotannins,lagerstroemin,and corosolic acid）in the green synthesis and capping of Au NPs. The green route synthesized Au NPs show strong photocatalytic activity in the reduction of dyes viz.,methylene blue,methyl orange,bromophenol blue and bromocresol green,and 4-nitrophenol under visible light in the presence of Na BH4. The non-toxic and cost effective LSE mediated Au NPs synthesis proposed in this study is extremely rapid compared to the other reported methods that require hours to days for complete synthesis of Au NPs using various plant extracts. Strong and stable photocatalytic behavior makes Au NPs attractive in environmental applications,particularly in the reduction of organic pollutants in wastewater.

关键词： Green synthesis Gold nanoparticles Lagerstroemia speciosa Photocatalyst Dyes Nitroaromatic compound

Identification and characterization of GIP1, an Arabidopsis thaliana protein that enhances the DNA binding affinity and reduces the oligomeric state of G-box binding factors

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Cell Research 2005年第8期15卷 567-575页

作者： paul C. SEHNKE,Beth j. LAUGHNER,Carla R. LYERLY LINEBARGER,William B. GURLEY,Robert j. FERL Program in Plant Cellular and Molecular Biology Department of Horticultural Sciences University of Florida Gainesville FL USA 32611 Program in Plant Cellular and Molecular Biology Department of Horticultural Sciences University of Florida Gainesville FL USA 32611 Program in Plant Cellular and Molecular Biology Department of Horticultural Sciences University of Florida Gainesville FL USA 32611 Department of Microbiology and Cell Science University of Florida Gainesville FL USA 32611 Program in Plant Cellular and Molecular Biology Department of Horticultural Sciences University of Florida Gainesville FL USA 32611

Environmental control of the alcohol dehydrogenase (Adh) and other stress response genes in plants is in part brought about by transcriptional regulation involving the G-box cis-acting DNA element and bZIP G-box Binding Factors (GBFs). The mechanisms of GBF regulation and requirements for additional factors in this control process are not well understood. In an effort to identify potential GBF binding and control partners, maize GBF1 was used as bait in a yeast two-hybrid screen of an A. thaliana cDNA library. GBF Interacting Protein 1 (GIP1) arose from the screen as a 496 amino acid protein with a predicted molecular weight of 53,748 kDa that strongly interacts with GBFs. Northern analysis of A. thaliana tissue suggests a 1.8-1.9 kb GIP1 transcript, predominantly in roots. Immunolocalization studies indicate that GIP1 protein is mainly localized to the nucleus. In vitro electrophoretic mobility shift assays using an Adh G-box DNA probe and recombinant A. thaliana GBF3 or maize GBF1, showed that the presence of GIP1 resulted in a tenfold increase in GBF DNA binding activity without altering the migration, suggesting a transient association between GIP1 and GBF. Addition of GIP1 to intentionally aggregated GBF converted GBF to lower molecular weight macromolecular complexes and GIP1 also refolded denatured rhodanese in the absence of ATP. These data suggest GIP1 functions to enhance GBF DNA binding activity by acting as a potent nuclear chaperone or crowbar, and potentially regulates the multimeric state of GBFs, thereby contributing to bZIP-mediated gene regulation.

关键词： G-box binding factor interacting protein nuclear chaperone DNA binding affinity.

MicroRNA Signaling Pathway Network in Pancreatic Ductal Adenocarcinoma

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journal of Genetics and Genomics 2015年第10期42卷 563-577页

作者： Longhao Sun Corrine Ying Xuan Chua Weijun Tian Zhixiang Zhang paul j.Chiao Wei Zhang Department of Pathology The University of Texas MD Anderson Cancer Center Department of General Surgery Tianjin Medical University General Hospital The University of Texas Graduate School of Biomedical Sciences Department of Molecular and Cellular Oncology The University of Texas MD Anderson Cancer Center

Pancreatic ductal adenocarcinoma （PDAC） is considered to be the most lethal and aggressive malignancy with high mortality and poor prognosis. Their responses to current mnltimodal therapeutic regimens are limited. It is urgently needed to identify the molecular mechanism underlying pancreatic oncogenesis. Twelve core signaling cascades have been established critical in PDAC tumorigenesis by governing a wide variety of cellular processes. MicroRNAs （miRNAs） are aberrantly expressed in different types of tumors and play pivotal roles as post-transcriptional regulators of gene expression, Here, we will describe how miRNAs regulate different signaling pathways that contribute to pancreatic oncogenesis and progression,

关键词： MicroRNA Signaling pathway Tumorigenesis Pancreatic ductal adenocarcinoma