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Application of DNA barcodes in Hedyotis L.(Spermacoceae,Rubiaceae)

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Journal of Systematics and Evolution 2011年第3期49卷 203-212页

作者： Mark P.SIMMONS paul pui-hay but Pang-Chui SHAW Department of Biology Colorado State University School of Life Sciences The Chinese University of Hong Kong

The potential application of DNA barcodes of plastid(matK,trnH-psbA,petD,and rbcL) and nuclear(internal transcribed spacer(ITS) of rDNA) DNA regions was investigated for 25 Hedyotis *** ITS showed the best species discrimination by resolving 23 of the species as exclusive lineages with no shared alleles between any of the 24 distinct species(*** and *** are not supported as distinct species based on our molecular and morphological data).Conversely,rbcL performed the worst and only resolved 10 of the species as exclusive lineages,and 10 species with shared *** ITS has the advantage of high PCR amplification success and it provides good intra-and interspecific variation distribution *** most powerful plastid markers were petD and trnH-psbA,but we could amplify and sequence trnH-psbA for only 83% of the accessions *** of ITS and petD performed extremely well,with all 24 of the distinct species resolved as exclusive lineages and no shared alleles between any of the distinct *** therefore recommend ITS,or a combination of ITS and petD,as the standard DNA barcode in Hedyotis,but acknowledge that there are no shared alleles between distinct species for matK and rbcL combined.

关键词： DNA barcode Hedyotis molecular identification Rubiaceae taxonomy.

Contribution of molecular cladistics to the taxonomy of Rutaceae in China

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Journal of Systematics and Evolution 2009年第2期47卷 144-150页

作者： paul pui-hay but Alice Wing-Sem POON Pang-Chui SHAW Mark P.SIMMONS Harald GREGER Department of Biology Chinese University of Hong Kong Department of Biochemistry Chinese University of Hong Kong Department of Biology Colorado State UniversityFort Collins Comparative and Ecological Phytochemistry Faculty Center of BotanyUniversity of Vienna

The treatment of Rutaceae in the Chinese flora chose to follow Engler in recognizing Rutoideae and Toddalioideae as two separate *** and chemical comparisons,however,suggested group-ing those two subfamilies in one subfamily,*** move has received support from molecular phyloge-netic analyses,which also showed that the Chinese taxa in Euodia should be placed in Tetradium and Melicope following *** into the chemistry and molecular phylogeny of Murraya also indicated that the species in the section Bergera without yuehchukene should be removed from *** findings clearly show the value of molecular cladistics to the taxonomy of Rutaceae in China and also directions for further inves-tigations.

关键词： Bergera Euodia Melicope Murraya Rutaceae Rutoideae Tetradium Toddalioideae

QUALITY CONTROL FOR CHINESE HERBAL DRUGS USING DNA PROBE TECHNOLOGY

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中国实验方剂学杂志 2002年第S1期8卷 -页

作者： Katsuko KOMATSU paul pui hay BUT

ＩＮＴＲＯＤＵＣＴＩＯＮＴｈｅｔｙｐｅａｎｄｓｐｅｃｔｒｕｍｏｆｄｉｓｅａｓｅｓａｒｅｃｈａｎｇｉｎｇｓｉｇｎｉｆｉｃａｎｔｌｙａｓｔｈｅｓｏｃｉｅｔｙａｇｉｎｇｔｏｄａｙ .Ｔｈｅａｕｔｏｉｍｍｕｎｅｄｉｓｅａｓｅｓ...

ＩＮＴＲＯＤＵＣＴＩＯＮＴｈｅｔｙｐｅａｎｄｓｐｅｃｔｒｕｍｏｆｄｉｓｅａｓｅｓａｒｅｃｈａｎｇｉｎｇｓｉｇｎｉｆｉｃａｎｔｌｙａｓｔｈｅｓｏｃｉｅｔｙａｇｉｎｇｔｏｄａｙ .Ｔｈｅａｕｔｏｉｍｍｕｎｅｄｉｓｅａｓｅｓｓｕｃｈａｓｓｅｎｉｌｅｄｅｍｅｎｔｉａ ,ＡＩＤＳ ,ａｓｗｅｌｌａｓｃａｒｄｉｏｃｅｒｅｂｒａｌｖａｓｃｕｌａｒｄｉｓｅａｓｅｓｓｕｃｈａｓｈｙｐｅｒｔｅｎｓｉｏｎ ,ａｒｒｈｙｔｈｍｉａ ,ｍｙｏｃａｒｄｉａｃｉｎｆｒａｃｔｉｏｎｅａｒｅｂｅｃｏｍｉｎｇａｎｉｎｔｒａｃｔａｂｌｅａｎｄｇｌｏｂｕｌａｒｐｒｏｂｌｅｍ .Ｒｅｃｅｎｔｌｙ ,ｐｅｏｐｌｅａｒｅｖｅｒｙｍｕｃｈｃｏｎｃｅｒｎｅｄｗｉｔｈｓｉｄｅｅｆｆｅｃｔｓｏｆｓｙｎｔｈｅｔｉｃｐｈａｒｍａｃｅｕｔｉｃａｌｓａｎｄａｒｅａｎｘｉｏｕｓｔｏｒｅｔｕｒｎｔｏｔｈｅｕｓｅｏｆｎａｔｕｒａｌｍｅｄｉｃｉｎｅ .Ｂａｃｋｔｏｎａｔｕｒｅ ,ｔｈｅｎｅｅｄｆｏｒＣｈｉｎｅｓｅｈｅｒｂａｌｄｒｕｇｓｉｓｉｎｃｒｅａｓｉｎｇｇｒａｄｕａｌｌｙｆｏｒｐｒｅｖｅｎｔｉｏｎａｎｄｔｈｅｒａｐｙｏｆｄｉｓｅａｓｅｓｉｎｔｈｅｗｏｒｌｄ .Ａｒｅｃ...

关键词：

A Special Form of Asexual Reproduction in Octoblepharum Albidum

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A Special Form of Asexual Reproduction in Octoblepharum Albi...

第七届全国系统与进化植物学青年学术研讨会

作者： paul pui-hay but Department of Biology The Chinese University of Hong KongShatinNTHong Kong.

Populations of Octoblepharum albidum in very moist and shaded habitats were found to have two ways for asexual reproduction. The first one is the production of gemmae along the upper half of leaf margins. The second f... 详细信息

关键词： Asexual reproduction bud rhizoid gemma Octoblepharum albidum rhizoidal bud walking moss

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植物标本室代号

广西植物 1988年第1期 65-74页

作者：毕培曦香港中文大学

毕培曦博士在本文中提出我国植物标本室代号的统一问题很值得注意,应该求得解决,达到国内、外使用统一代号,以利中外学术交流,促进本学科发展。至于如何解决,希望同行们提出宝贵意见,写给本刊编辑部,以便刊出,公开讨论。

关键词： Herbarium Code Taxonomy

水杨梅属植物化学成分及其生物活性的研究进展(英文)

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药学学报 2000年第7期35卷 552-558页

作者：明东升王峰徐宏喜董辉毕培曦香港中文大学中药研究中心中国药品生物制品检定所北京100050

There are about 70 species in the genus Geum, of these 6 species are found in China[1～4]. Some species, such as *** Jacq, *** Thunb. and *** Thunb.

关键词：水杨梅生物活性鞣质三萜黄酮化学成分

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DNA探针技术在中药材品质监控方面的研究与应用(英文)

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DNA探针技术在中药材品质监控方面的研究与应用(英文)

作者：刘玉萍曹晖小松かつ子 paul pui hay BUT Research Center for Ethnomedicines Depatment of Biology and Institute of Chinese medicine

INTRODUCTION The type and spectrum of diseases are changing significantly as the society aging today. The autoimmune diseases such as senile dementia,AIDS,as well as cardio-cerebral vascular diseases such as hypertension,arrhythmia,myocardiac infractione are becoming an intractable and globular ***,people are very much concerned with side effects of synthetic pharmaceuticals and are anxious to return to the use of natural *** to nature,the need for Chinese herbal drugs is increasing gradually for prevention and therapy of diseases in the world. A recent survey in China has found about 10000 species of medicinal plants and animals, and about 500 of them are frequently *** accurate identification of these medicinal materials is a prerequisite for the quality control of Chinese herbal *** means of authentication rely on the inspection of organoleptic markers such as shape,color,texture and odor

关键词： Chinese herbal drugs DNA chip DNA probe quality control

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治疗鼻炎中药复方制剂的抗过敏作用

时珍国医国药 2007年第12期18卷 3026-3027页

作者：夏玉凤戴岳毕培曦陈耀邦中国药科大学中药学院江苏南京210038 香港中文大学中医中药研究所

目的观察4种治疗鼻炎的中药复方制剂对速发型过敏反应的影响,分析其中的有效中药。方法抗原攻击前1 h,给小鼠口服受试药物,观察其对异种被动皮肤过敏反应的作用;体外实验中,将药物与大鼠腹腔肥大细胞共同温孵,观察其对compound 48/80诱... 详细信息

目的观察4种治疗鼻炎的中药复方制剂对速发型过敏反应的影响,分析其中的有效中药。方法抗原攻击前1 h,给小鼠口服受试药物,观察其对异种被动皮肤过敏反应的作用;体外实验中,将药物与大鼠腹腔肥大细胞共同温孵,观察其对compound 48/80诱导组胺释放的影响。结果速效鼻敏感丸、林金生鼻敏清胶囊及和春堂鼻敏清片明显抑制小鼠异种被动皮肤过敏反应,减少compound 48/80诱导的组胺释放,而速效鼻敏素胶囊对被动皮肤过敏反应无明显影响;速效鼻敏感丸及和春堂鼻敏清片主要组成药物九里明、卷柏和苍耳子显著抑制小鼠异种被动皮肤过敏反应,而白芷、辛夷和决明子无明显作用。结论速效鼻敏感丸、和春堂鼻敏清片及林金生鼻敏清胶囊具有抗过敏活性,九里明和卷柏可能为速效鼻敏感丸及和春堂鼻敏清片的有效组分,苍耳子亦可能为速效鼻敏感丸的有效组份之一。

关键词：鼻炎被动皮肤过敏反应肥大细胞组胺

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纪念蒋英教授(英文)

武汉植物学研究 1986年第1期 69-78页

作者：李秉滔毕培曦华南农业大学香港中文大学

蒋英教授是著名的植物分类学家。他从事中国夹竹桃科、萝藦科、番荔枝科植物的专门研究和教育事业有五十四年历史,为祖国科学事业奋斗一生,作出了重大贡献。本文详细地介绍了他的生平、艰苦创业的经过、严谨治学精神、培养人才的经验,... 详细信息

蒋英教授是著名的植物分类学家。他从事中国夹竹桃科、萝藦科、番荔枝科植物的专门研究和教育事业有五十四年历史,为祖国科学事业奋斗一生,作出了重大贡献。本文详细地介绍了他的生平、艰苦创业的经过、严谨治学精神、培养人才的经验,并总结了他一生的科研成就和八十多篇著作名录,供后人参考。

关键词：蒋英