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Improved Culture Media for Embryogenic Callus Generation in Sorghum [Sorghum bicolor (L.) Moench]

Phyton-International Journal of Experimental Botany 2020年第1期89卷 111-119页

作者： paul chege Andrea Palagyi Csaba Lantos Erzsebet Kiss Janos Pauk Szent Istvan University Doctoral School of Plant ScienceGodolloPater K.u.1.H-2103Hungary Cereal Research Non-profit Ltd. Department of BiotechnologySzegedAlsokikotosor 9.H-6701Hungary

Many attempts on optimization of sorghum[Sorghum bicolor(L.)Moench]tissue culture induction media have been made,but the culture system remains with some bottlenecks compared to that of other *** study aimed at assessing the suitability of various induction media to produce embryogenic callus(yellow and friable)with high induction rates and reduced phenolic *** six culture medium modifications:3 based on Murashige and Skoog(MS)medium and one each based on Chu N6,Gamborg B5 and 190-2 media respectively were applied in the culture of mature embryos from 10 sorghum *** there was a genotype influence on the attainment of a yellow callus,friability of the callus was determined to be dependent on the culture medium and not the *** strength MS medium with 0.2 mg/l 2,4-D with 2.8 g/l Gelrite®as the gelling agent modified with 1.0 g/l KH_(2)PO_(4),1.0 g/l L-proline,1.0 g/l L-asparagine and 0.16 mg/l CuSO_(4)·5H_(2)O(type E)was found to be the most effective resulting in about 60%yellow coloured callus induction with 25%*** of CuSO_(4)·5H_(2)O,KH_(2)PO_(4),L-proline and L-asparagine significantly reduced the phenolic *** strength MS medium was observed to contribute to quality callus production when compared to full strength MS media modified with the *** half strength MS medium was also observed to suppress phenolic *** 190-2 produced the highest regeneration frequency(40%)among the 3-regeneration media *** results provide information on a suitable sorghum callus induction medium necessary for embryogenesis.

关键词： Sorghum medium embryogenic callus half-strength MS

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Doubled Haploid Production Using an Improved Anther Culture Protocol for Sorghum[Sorghum bicolor(L.)Moench]

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Phyton-International Journal of Experimental Botany 2021年第2期90卷 475-487页

作者： paul chege Erzsébet Kiss Csaba Lantos Andrea Palágyi János Pauk Cereal Research Non-profit Ltd. Alsókikötősor 9Szeged6726Hungary Doctoral School of Plant Science Szent István UniversityGödöllőH2103Hungary

Sorghum[Sorghum bicolor(L.)Moench]can benefit from accelerated breeding and release of improved varieties through doubled haploid *** technology has been used in speeding up the breeding of other major cereals such as wheat,maize and rice,for which generally widely applied optimised protocols exist.A reproducible protocol for the crop,that can overcome genotype dependency and other species-specific challenges such as phenolic exudation is however *** study aimed at sorghum doubled haploids production thereby contributing to the development of an improved *** the 28 hybrid genotypes,both F1 registered-and experimental hybrids involved,this study successfully produced haploids from five genotypes and subsequently,four confirmed doubled-haploid lines on W14mf medium or its modification with 1.0 gl^(-1)L-proline,1.0 gl^(-1)L-asparagine and 1.0 gl^(-1)KH_(2)PO_(4).Medium 190-2Cu was used for regeneration and rooting,which occurred successfully,if the calli were transferred on to it less than 7 days after induction,and temperature was maintained at 25°C under light *** dependency was not wholly overcome;however,sorghum’s high tillering ability and abiotic stress tolerance were observed to contribute to attainment of haploid *** diploids producing seeds at rates of upto 80.5%were obtained,therefore eliminating the need for colchicine duplication.

关键词： In vitro androgenesis doubled-haploid sorghum anther-culture breeding

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