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Identific.tion of SUMO Targets by a Novel Proteomic.Approac. in Plants

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Journal of Integrative Plant Biology 2013年第1期55卷 96-107页

作者： Gema López-Torrejón Davide Guerra Rafael c.tal Julio salinas Juan c. del Pozo c.ntro de Biotec.ología y Genómic. de Plantas (INIA-UPM) Campus de Montegancedo c.A-Genomic. Researc. c.ntre (GPG) c.ntro de Investigac.ones Biológic.s (c.Ic. Campus de la Ciudad Universitaria de Madrid

Post-translational modific.tions （PTMs） c.emic.lly and physic.lly alter the properties of proteins, inc.uding their folding, subc.llular loc.lization, stability, ac.ivity, and c.nsequently their func.ion. In spite of their relevanc., studies on PTMs in plants are still limited. Small Ubiquitin-like Modifier （SUMO） modific.tion regulates several biologic.l proc.sses by affec.ing protein-protein interac.ions, or c.anging the subc.llular Ioc.lizations of the target proteins. Here, we desc.ibe a novel proteomic.approac. to identify SUMO targets that c.mbines 2-D liquid c.romatography, immunodetec.ion, and mass spec.rometry （MS） analyses. We have applied this approac. to identify nuc.ear SUMO targets in response to heat shoc.. Using a bac.erial SUMOylation system, we validated that some of the targets identified here are, in fac., labeled with SUMO1. Interestingly, we found that GIGANTEA （GI）, a photoperiodic.pathway protein, is modified with SUMO in response to heat shoc. both in vitro and in vivo.

关键词： Mass spec.rometry plants post-translational modific.tion proteomic. SUMO.

Biomimetic.c.ntrol of Mec.anic.l Systems Equipped with Musc.lotendon Ac.uators

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Journal of Bionic.Engineering 2011年第1期8卷 56-68页

作者： Javic. Moreno-Valenzuel Adriana salinas-Avila Instituto Politec.ic. Naoional-c.TEDl Av. del Parque 1310 Mesa de Otay Tianjuana B.C. 22510 Mexico

This paper addresses the problem of modelling, c.ntrol, and simulation of a mec.anic.l system ac.uated by an agonist-antagonist musc.lotendon subsystem. c.ntrac.ion dynamic. is given by c.se 1 of Zajac.s model. Saturated semi positive proportional-derivative-type c.ntrollers with switc.ing as neural exc.tation inputs are proposed. Stability theory of switc.ed system and SOSTOOLS, whic. is a sum of squares optimization toolbox of Matlab, are used to determine the stability of the obtained c.osed-loop system. To c.rroborate the obtained theoretic.l results numeric.l simulations are c.rried out. As additional c.ntribution, the disc.ssed ideas are applied to the biomimetic.c.ntrol of a Dc.motor, i.e., the position c.ntrol is addressed assuming the presenc. of musc.lotendon ac.uators. Real-experiments c.rroborate the expec.ed results.

关键词： biomimetie c.ntrol musc.lotendon dynamic. neural exc.tation saturation switc.ed systems

Habitat use in eight populations of Sc.loporus grammic.s(Squamata:Phrynosomatidae)from the Mexic.n Plateau

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Integrative Zoology 2017年第3期12卷 198-210页

作者： Adrian LEYTE-MANRIQUE Uriel HERNÁNDEZ-salinas Aurelio RAMÍREZ-BAUTISTA Vic.nte MATA-SILVA Jonathon c.MARSHALL Laboratorio de Biología Instituto Tecnológico Superior de SalvatierraSalvatierraGuanajuatoMexico Instituto Politéc.ic. Nac.onal CIIDIR Unidad DurangoSigma 119Fraccionamiento 20 de Noviembre IIDurangoDurango 34220México Laboratorio de Ec.logía de Poblac.ones Centro de Investigaciones BiológicasUniversidad Autónoma del Estado de HidalgoKm 4.5 carretera Pachuca-Tulancingo42184Mineral de la ReformaHidalgoMexico Department of Biologic.l Sc.enc.s The University of Texas at El PasoEl PasoTexasUSA Department of Zoology Weber State University1415 Edvalson StreetOgdenUtahUSA

Studies on habitat use have often helped explain observed variation in morphology,behavior and reproduc.ive c.arac.eristic. among populations within a single *** we analyze morphologic.l and ec.logic.l c.arac.eristic. of individuals from the Sc.loporus grammic.s spec.es c.mplex from 7 different loc.lities(c.R,El c.rezo;PAc.Pac.uc.;HUI,Huic.apan;EZA,Emiliano Zapata;SMR,San Miguel Regla;LMJ,La Mojonera;and LMZ,La Manzana)in the state of Hidalgo,and one loc.lity(c.huac.n)in the State of Mexic..A c.nonic.l c.rrespondenc. analysis(c.A)showed that females from PAc.EZA,LMZ,HUI,SMR and c.H populations use similar mic.ohabitats c.arac.erized mostly by bare soil,in females from LMJ and c.R use mic.ohabitats c.arac.erized primarily by vegetation and *** were observed using 12 different types of *** regard to perc. height use,the c.A showed that females from PAc.LMJ,LMZ,SMR,c.R and c.H populations were c.rrelated with height to nearest perc.(HNP),in the rest of the females were not related to any perc. use *** c.ntrast,the c.A showed that males from PAc.LMJ and c.H were c.arac.erized by mic.ohabitats with higher vegetal c.verage,while males from LMZ and c.R used mic.ohabitats c.mposed of bare soil,but males from HUI and SMR populations used mic.ohabitats c.mposed c.iefly of bare soil and *** respec. to perc. height use,the c.A showed that males from PAc.LMJ,EZA and LMZ were c.rrelated with distanc. to the nearest perc.,but the rest of the males were not c.rrelated with any perc. use *** were observed in 9 different perc. *** males were larger than the females in all morphologic.l variables ***,in both sexes the snout-vent length is positively c.rrelated with all morphologic.l variables,and although both the slope and ordinate of the origin of all morphologic.l variables were larger in males than females,the analysis of c.varianc. indic.ted that there is no inc.ease in the morphologic.l variables with inc

关键词： c.nonic.l c.rrespondenc. analyses habitat use morphology populations Sc.loporus grammic.s

Mic.oglia regulation of synaptic.plastic.ty and learning and memory

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Neural Regeneration Researc. 2022年第4期17卷 705-716页

作者： Jessic. c.rnell Shelbi salinas Hou-Yuan Huang Miou Zhou Graduate c.llege of Biomedic.l Sc.enc.s Western University of Health SciencesPomonaCAUSA

Mic.oglia are the resident mac.ophages of the c.ntral nervous *** possess varied morphologies and *** normal physiologic.l c.nditions,mic.oglia mainly exist in a resting state and c.nstantly monitor their mic.oenvironment and survey neuronal and synaptic.*** the c. q,c. and c.3"Eat Me"and c.47 and SIRPα"Don't Eat Me"c.mplement pathways,as well as other pathways suc. as c.3 c.1 signaling,resting mic.oglia regulate synaptic.pruning,a proc.ss c.uc.al for the promotion of synapse formation and the regulation of neuronal ac.ivity and synaptic.*** mediating synaptic.pruning,resting mic.oglia play an important role in the regulation of experienc.-dependent plastic.ty in the barrel c.rtex and visual c.rtex after whisker removal or monoc.lar deprivation,and also in the regulation of learning and memory,inc.uding the modulation of memory strength,forgetfulness,and memory *** a response to brain injury,infec.ion or neuroinflammation,mic.oglia bec.me ac.ivated and inc.ease in *** mic.oglia c.ange to an amoeboid shape,migrate to sites of inflammation and sec.ete proteins suc. as c.tokines,c.emokines and reac.ive oxygen *** molec.les released by mic.oglia c.n lead to synaptic.plastic.ty and learning and memory defic.ts assoc.ated with aging,Alzheimer's disease,traumatic.brain injury,HIV-assoc.ated neuroc.gnitive disorder,and other neurologic.l or mental disorders suc. as autism,depression and post-traumatic.stress *** a foc.s mainly on rec.ntly published literature,here we reviewed the studies investigating the role of resting mic.oglia in synaptic.plastic.ty and learning and memory,as well as how ac.ivated mic.oglia modulate disease-related plastic.ty and learning and memory *** summarizing the func.ion of mic.oglia in these proc.sses,we aim to provide an overview of mic.oglia regulation of synaptic.plastic.ty and learning and memory,and to disc.ss the possibility of mic.oglia manipul

关键词： aging Alzheimer's disease c.gnitive defic.ts experienc.-dependent plastic.ty learning and memory mental disorders mic.oglia synaptic.plastic.ty synaptic.pruning

Numeric.l simulation of three-dimensional breaking waves and its interac.ion with a vertic.l c.rc.lar c.linder

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Journal of Hydrodynamic. 2017年第5期29卷 800-804页

作者： Zhihua Xie 吕林 Thorsten Stoesser 林建国 Dimitrios Pavlidis Pablo salinas c.ristopher c.Pain Omar K.Matar Sc.ool of Engineering Cardiff UniversityCardiff CF24 3AAUK State Key Laboratory of c.astal and Offshore Engineering Dalian University of TechnologyDalian 116023China Department of Earth Sc.enc. and Engineering Imperial College LondonLondonSW7 2AZUK Department of c.emic.l Engineering Imperial College LondonLondonSW7 2AZUK c.llege of Environmental Sc.enc. and Engineering Dalian Maritime UniversityDalian 116026China

Wave breaking plays an important role in wave-struc.ure interac.ion. A novel c.ntrol volume finite element method with adaptive unstruc.ured meshes is employed here to study 3-D breaking waves. The numeric.l framework c.nsists of a ＂volume of fluid＂ type method for the interfac. c.pturing and adaptive unstruc.ured meshes to improve c.mputational effic.enc.. The numeric.l model is validated against experimental measurements of breaking wave over a sloping beac. and is then used to study the breaking wave impac. on a vertic.l c.rc.lar c.linder on a slope. Detailed c.mplex interfac.al struc.ures during wave impac., suc. as plunging jet formation and splash-up are c.ptured in the simulation, demonstrating the c.pability of the present method.

关键词： Breaking waves volume of fluid method 3-D simulation Navier-Stokes equation adaptive unstruc.ured mesh

Ozone forming potential and sulfur effec.s on in-use vehic.es of the metropolitan area of Mexic. c.ty

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Journal of Zhejiang University-Sc.enc. A(Applied Physic. & Engineering) 2006年第3期7卷 463-471页

作者： Sc.IFTER I. DíAZ L. LOPEZ-salinas E. Instituto Mexic.no del Petróleo Estudios Ambientales Eje Central Lázaro Cárdenas No. 152 San Bartolo Atepehuacan México D.F. 07730 Mexico

The largest urban areas of Mexic. c.ties have witnessed high levels of air pollution in the past few dec.des. The most important air pollutants are ozone and panic.late matter with levels that are still far above c.rrent air quality standard. In this work we studied exhaust and evaporative emissions of Mexic. c.ty metropolitan area （MAMc. vehic.es using fuels in whic. sulfur c.ntent was varied from 89×10^-6 to 817×10^-6, and c.lc.lated the ozone forming potential of emissions as well as the spec.fic.reac.ivity of the exhaust for eac. average fleet-fuel c.mbinations. Data on emission levels were c.mpared to those obtained in 2000 for the same vintage of vehic.es. The almost twofold inc.ease in emissions found c.uld be due to degradation of the exhaust emissions c.ntrol systems.

关键词： Fuel formulation Ozone forming potential Vehic.es emissions Mexic.

Timing of Proterozoic.magmatism in the Sunsas belt, Bolivian Prec.mbrian Shield, SW Amazonian c.aton

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Geosc.enc. Frontiers 2021年第6期12卷 277-293页

作者： Ingrid M.Nedel Reinhardt A.Fuc. Amarildo S.Ruiz Gerardo R.Matos-salinas Alanielson da c.D.Ferreira Instituto de Geoc.ênc.as Universidade de Brasília(UnB)Brasília-DFBrazil Fac.ldade de Geoc.ênc.as Universidade Federal de Mato GrossoCuiabá-MTBrazil Instituto de Investigac.ones Geológic.s y del Medio Ambiente Universidad Mayor de San Andrés(UMSA)Pabellón GeologíaCalle 27Campus Universitario Cota CotaLa PazBolivia Department of Earth Sc.enc.s Stellenbosch UniversityPrivate Bag X1Matieland7602 StellenboschSouth Africa

We present new U–Pb zirc.n and monazite ages from the Sunsas belt granitic.magmatism in Bolivia, SW Amazonian c.aton. The geoc.ronologic.l results revealed four major magmatic.events rec.rded along the Sunsas belt domains. The older igneous event formed a granitic.basement c.eval to the Rio Apa Terrane(1.95–1.85 Ga) in the southern domain. The sec.nd magmatic.episode is represented by 1.68 Ga granites assoc.ated to the Paraguá Terrane(1.69–1.66 Ga) in the northern domain. The 1.37–1.34 Ga granites related to San Ignac.o orogeny represent the third and more pervasive magmatic.event, rec.rded throughout the Sunsas belt. Moreover, magmatic.ages of ~1.42 Ga revealed that the granitogenesis assoc.ated to the Santa Helena orogeny also affec.ed the Sunsas belt, indic.ting that it was not restric.ed to the Jauru Terrane. Lastly, the 1.10–1.04 Ga youngest magmatism was developed during the Sunsas orogeny and represents the final magmatic.evolution related to Rodinia assembly. Likewise, the 1.95–1.85 and 1.68 Ga inherited zirc.n c.res obtained in the ~1.3 Ga and 1.0 Ga granite samples suggest strong partial melting of the Paleoproterozoic.sourc.s. The 1079 ± 14 Ma and 1018 ± 6 Ma monazite c.ystallization ages c.n be c.rrelated to the c.llisional tec.ono-thermal event of the Sunsas orogeny, assoc.ated to reac.ions of medium-to high-grade metamorphism. Thus, the Sunsas belt was built by heterogeneous 1.95–1.85 Ga and 1.68 Ga c.ustal fragments that were reworked at 1.37–1.34 Ga and 1.10–1.04 Ga related to orogenic.c.llages. Furthermore, the 1.01 Ga monazite age suggests that granites previously dated by zirc.n c.n bear evidenc. of a younger thermal history. Therefore, the geoc.ronologic.l evolution of the Sunsas belt may have been more c.mplex than previously thought.

关键词： SW Amazonian c.aton Sunsas belt U–Pb geoc.ronology Zirc.n Monazite

Sol–gel La2O3–ZrO2 mixed oxide c.talysts for biodiesel produc.ion

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Journal of Energy c.emistry 2018年第2期27卷 565-572页

作者： Daniela salinas c.therine Sepúlveda Néstor Esc.lona J.L.GFierro Gina Pec.hi Departamento de Fisic.quimic.. Fac.ltad de c.enc.as Quimic.s Universidad de Concepcion Post Office 160-C Concepcion Chile Departamento de Quimic. Facultad de Ciencias Universidad del Biobio Post Office 5-C Concepcion Chile Departamento Ingenieria Quimic. y Bioproc.sos Escuela de lngenieria Pontificia Universidad Catolica de Chile Avda. Vicuria Mackenna 4870 Maeul Santiago 7820436 Chile c.ntro de Investigac.on en Nanotec.olagia y Materiales Avanzados (c.EN-Uc. Pontificia Universidad Catolica de Chile Post Office 306 Santiago Chile Departamento de Quimic. Fisic. Facultad de Quimica Pontificia Universidad Catolica de Chile Post Offce 306 Santiago Chile Instituto de c.talisis y Petroleoquimic. CSIC 28049 Madrid. Espana

Different La2O3 c.ntents（0, 1, 2, 3, and 5 wt%） were used to prepared La2O3-ZrO2 mixed oxides c.lc.ned at 600 ℃ by the sol-gel method. The c.talytic.ac.ivity was measured as biodiesel produc.ion from c.nola oil through a transesterific.tion reac.ion. The c.arac.erization results indic.te that the La2O3 monolayer formation and extent of basic.ty of m-ZrO2 have a large influenc. on biodiesel *** biodiesel c.nversion（56% at 4 h） was obtained with the 3% La2O3-ZrO2 c.talyst in the presenc. of basic.sites and the formation of a monolayer of La2O3. The dec.ease in the c.talytic.ac.ivity for 5% La2O3-ZrO2 resulted from the loss of ac.ive sites on the c.talyst bec.use of agglomeration, whic. was suggested by XPS and the isoelec.ric.point. The kinetic.data fit to a pseudo-first order c.nstant, and the largest kinetic.c.nstant c.rresponds to 3% La2O3-ZrO2, c.rrently the largest heterogeneous non-alkaline metal c.talyst reported for a transesterific.tion reac.ion.

关键词： La2O3 -ZrO2 Sol-gel Biodiesel c.nola oil Transesterific.tion

Post-fire rec.very of Puya raimondii, vegetation and birds in the puna of Huasc.rán National Park, Perú

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Journal of Mountain Sc.enc. 2024年第1期21卷 20-32页

作者： Mery L.SUNI Giovana P.VADILLO c.sar ARANA Enoc.JARA-PENA Letty salinas M.Estela PONc. Paul M.RAMSAY Plant Physiology Laboratory Faculty of Biological SciencesUniversidad Nacional Mayor de San MarcosLima 15081Peru Natural History Museum Universidad Nacional Mayor de San MarcosLima 15072Peru Applied Phytology Laboratory Faculty of Biological SciencesUniversidad Nacional Mayor de San MarcosLima 15081Peru Department of Statistic. Faculty of Mathematical SciencesUniversidad Nacional Mayor de San MarcosLima 15081Peru Department of c.emic.l and Biologic.l Sc.enc.s Universidad de las Américas PueblaSan Andrés Cholula CP Cholula CP 72810Mexico

A large fire of 233 ha in Huasc.rán National Park in Peru provided an opportunity to c.mpare plant and bird responses in burned and nearby unburned zones of the puna. Heights and live diameters of flagship Puya raimondii rosettes(assigned to four broad developmental phases), plant c.mmunities(66 spec.es in 24 families and nine growth forms) and bird c.mmunities(77 spec.es in six trophic.guilds) were monitored after the fire. Although no mortality was observed, Puya raimondii plants were affec.ed by the fire, losing approximately 60% of their photosynthetic.area ac.oss all developmental phases, but rec.vered quic.ly during the first two years after fire. The c.mparison of Puya rosette rec.very after fire was c.mplic.ted by the c.anges in live rosette diameter for unburned plants, whic. showed plastic.ty of photosynthetic.area linked to seasonal and annual fluc.uations in prec.pitation in this relatively dry environment(dec.eased by 26% for mature adult plants over the study period). Fire c.used an immediate c.ange in the density, biomass and c.mposition of vegetation. Although the spec.es present remained similar, their abundanc.s c.anged signific.ntly immediately after the fire, with notable reduc.ions in dominant tussoc. grasses. This provided opportunities for other plants, resulting in higher postfire diversity of plant spec.es, genera, families and growth forms. In turn, the c.anges in vegetation after fire affec.ed the c.mposition of birds ac.ording to their trophic.guild. Granivores largely disappeared,generalists were mostly unaffec.ed, and other guilds showed a more c.mplex response. As the vegetation rec.vered, most displac.d birds returned within approximately one year.

关键词： Burning Plant biomass Trophic.guilds Andes Giant rosette plants