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Plant cell wall-mediated disease resistance:Current understanding and future perspectives

Molecular Plant 2024年第5期17卷 699-724页

作者： Antonio molina Lucıa Jorda Miguel Angel Torres Marina Martın-Dacal Diego Jose Berlanga patricia Fernandez-Calvo Elena Gomez-Rubio Sonsoles Martın-Santamarıa Centro de Biotecnología y Genómica de Plantas Universidad Politécnica de Madrid(UPM)-Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria(INIA/CSIC)Campus de Montegancedo UPMPozuelo de Alarcon(Madrid)Spain Departamento de Biotecnologıa-Biología Vegetal Escuela Técnica Superior de Ingenierıa AgronomicaAlimentarıa y de BiosistemasUPMMadridSpain Centro de Investigaciones Biologicas Margarita Salas Consejo Superior de Investigaciones Científicas(CSIC)Ramiro de Maeztu 928040 MadridSpain Misión Biológica de Galicia Consejo Superior de Investigaciones Científicas(CSIC)Campus de Montegancedo UPMPozuelo de Alarcón(Madrid)Spain

Beyond their function as structural barriers,plant cell walls are essential elements for the adaptation of plants to environmental *** walls are dynamic structures whose composition and integrity can be altered in response to environmental challenges and developmental *** wall changes are perceived by plant sensors/receptors to trigger adaptative responses during development and upon stress *** cell wall damage caused by pathogen infection,wounding,or other stresses leads to the release of wall molecules,such as carbohydrates(glycans),that function as damage-associated molecular patterns(DAMPs).DAMPs are perceived by the extracellular ectodomains(ECDs)of pattern recognition receptors(PRRs)to activate pattern-triggered immunity(PTI)and disease ***,glycans released from the walls and extracellular layers of microorganisms interacting with plants are recognized as microbe-associated molecular patterns(MAMPs)by specific ECD-PRRs triggering PTI *** number of oligosaccharides DAMPs/MAMPs identified that are perceived by plants has increased in recent ***,the structural mechanisms underlying glycan recognition by plant PRRs remain ***,this knowledge is mainly focused on receptors of the LysM-PRR family,which are involved in the perception of various molecules,such as chitooligosaccharides from fungi and lipo-chitooligosaccharides(i.e.,Nod/MYC factors from bacteria and mycorrhiza,respectively)that trigger differential physiological ***,additional families of plant PRRs have recently been implicated in oligosaccharide/polysaccharide *** include receptor kinases(RKs)with leucine-rich repeat and Malectin domains in their ECDs(LRR-MAL RKs),Catharanthus roseus RECEPTOR-LIKE KINASE 1-LIKE group(CrRLK1L)with Malectin-like domains in their ECDs,as well as wall-associated kinases,lectin-RKs,and *** characterization of structural basis of glycans recogniti

关键词： Arabidopsis thaliana cell wall damage-associated molecular patterns DAMPs disease resistance glycans oligosaccharides pattern-triggered immunity PTl pattern recognition receptor PRRs

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社区75—85岁高危女性2年平衡训练项目预防跌倒引起受伤的效果研究：Ossébo随机对照试验

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英国医学杂志中文版 2015年第11期18卷 634-635页

作者： Fabiemle EL-Khoury Bernard Cassou Aurélien Latouche Philippe Aegerter Marie-Aline Charles patricia dargent-molina 沈赟 Université Parls-Sud UMR-S1018 F-94807VillejuifFrance Université ParisDescartes UMR-S1153 F-75014 ParisFrance Inserm Centre de Recherche Epidémiologie et Statistique Sorbonne Paris Cité (CRESS)U1153 F-94807Villejuif France UVSQ UMR-S 1168Université VersaillesSt-Quentin-en-Yveli France Inserm VIMA :Vieillissement et Maladies ChroniquesU1168F-94807Villejuif France AP-HP H. pitalSainte PérineCentrede GérontologieF-75016 Paris France Conservatoire National des Arts et Metiers (Chain) Centre for Re.arch in Computer Science and Telecommtmications (Cédric) EA4629 Paris France Al AP-HP H. pital Ambroise Paré Unité de Recherche Clinique Département de Santé Publique Boulogne- Billancourt France 上海交通大学附属第六人民医院内分泌代谢科

目的在社区75～85岁跌倒及受伤高危风险的女性人群中，评价2年渐进式平衡训练项目对减少受伤性跌倒的效果。设计实用性、多中心、双臂、平行组、随机对照试验。背景法国16个中、大型城市中的20个研究点。参与者706名女性，年龄75—85岁... 详细信息

目的在社区75～85岁跌倒及受伤高危风险的女性人群中，评价2年渐进式平衡训练项目对减少受伤性跌倒的效果。设计实用性、多中心、双臂、平行组、随机对照试验。背景法国16个中、大型城市中的20个研究点。参与者706名女性，年龄75—85岁，居住在自己家中，维持平衡及步态的能力减退。这些女性被随机招募至试验干预组（锻炼项目，n=352）或对照组（非干预组，n=354）。

关键词：随机对照试验预防效果训练计划社区生活 OSS 平衡年龄妇女

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Autoantibodies against beta cells to predict early insulin requirements in pediatric patients with clinically diagnosed type 2 diabetes

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World Journal of Diabetes 2024年第8期15卷 1717-1725页

作者： Jorge M molina patricia G Medina Rita A Gomez Julia R Herrera Nancy L Martínez Brenda Hernández Yesenia García Department of Endocrinology Children’s Hospital Federico GomezMexico 06720Mexico National Medical Center"Siglo XXI" UMAE Hosp EspecialidadesUnidad Invest Med Epidemiol ClinMexican Social Security InstituteMexico 06720Mexico Research Division UMAE Hospital de EspecialidadesCentro Médico Nacional Siglo XXIMexican Institute of Social SecurityMexico 06720Mexico Epidemiology Research Unit in Endocrinology and Nutrition Federico Gómez Children’s HospitalMexico 06720Mexico Department of Endocrinology Comprehensive Health Unit for Trans PersonsMexico 11340Mexico

BACKGROUND Autoimmunity has emerged as a probable disease modifier in patients with clinically diagnosed type 2 diabetes mellitus(T2DM),that is,patients who have insulin resistance,obesity,and other cardiovascular risk factors,suggesting that the presence of glutamic acid decarboxylase(anti-GAD65),islet antigen 2(anti-IA2),and zinc transporter 8(anti-Zn8T)antibodies could have deleterious effects on beta cell function,causing failure and earlier requirement for insulin *** To evaluate anti-GAD65,anti-IA2 and anti-Zn8T as predictors of early insulin requirement in adolescents with a clinical diagnosis of *** This was a case–control study in patients with clinically diagnosed with T2DM(68 cases and 64 controls with and without early insulin dependence respectively),male and female,aged 12–18 ***,blood pressure,glucose,insulin,C-peptide,glycated hemoglobin A1c,and lipid profiles were *** was used to measure anti-GAD65,anti-IA2,and anti-Zn8T *** statistics,Pearson'sχ2 test,Student's t test,and logistic regression was performed.P<0.05 was considered statistically *** There were 132 patients(53.8%female),with a mean age was 15.9±1.3 years,and there was a disease evolution time of 4.49±0.88 *** presence of anti-GAD65,anti-IA2,and anti-Zn8T positivity was found in 29.5%,18.2%,and 15.9%,*** the groups by early or no insulin dependence showed that the group with insulin had a higher frequency of antibody positivity:anti-GAD65 odds ratio(OR):2.42(1.112–5.303,P=0.026);anti-IA2:OR:1.55(0.859–2.818,P=0.105);and anti-Zn8T:OR:7.32(2.039–26.279,P=0.002).CONCLUSION Anti-GAD65 positivity was high in our ***-GAD65 and anti-Zn8T positivity showed a significantly depleted beta cell reserve phenotype,leading to an increased risk of early insulin dependence.

关键词： Diabetes Obesity Adolescents Autoimmunity Beta cell Insulin requirements

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Spermiogram and sperm head morphometry assessed by multivariate cluster analysis results during adolescence （12-18 years） and the effect of varicocele

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Asian Journal of Andrology 2016年第6期18卷 824-830页

作者： Fernando Vasquez Caries Soler patricia Camps Anthony Valverde Almudena Garcia-molina Research Group on Reproductive and Sexual Health Health Sciences Division Universidad del Norte EO. Box 1569 Barranquilla Colombia Department of Functional Biology and Physical Anthropology University of Valencia 46100 Burjassot Valencia Spain R+D Department Proiser R+D Scientific Park University of Valencia 46980 Paterna Valencia Spain Technological Institute of Costa Rica Agronomy School Animal Reproduction Department San Carlos Campus 223-21001 San Carlos Costa Rica

This work evaluates sperm head morphometric characteristics in adolescents from 12 to 18 years of age, and the effect of varicocele. Volunteers between 150 and 224 months of age （mean 191, n = 87）, who had reached oigarche by 12 years old, were recruited in the area of Barranquilla, Colombia. Morphometric analysis of sperm heads was performed with principal component （PC） and discriminant analysis, Combining seminal fluid and sperm parameters provided five PCs： two related to sperm morphometry, one to sperm motility, and two to seminal fluid components. Discriminant analysis on the morphometric results of varicocele and nonvaricocele groups did not provide a useful classification matrix. Of the semen-related PCs, the most explanatory （40%） was related to sperm motility. Two PCs, including sperm head elongation and size, were sufficient to evaluate sperm morphometric characteristics. Most of the morphometric variables were correlated with age, with an increase in size and decrease in the elongation of the sperm head. For head size, the entire sperm population could be divided into two morphometric subpopulations, SP1 and SP2, which did not change during adolescence. In general, for varicocele individuals, SP1 had larger and more elongated sperm heads than SP2, which had smaller and more elongated heads than in nonvaricocele men. In summary, sperm head morphometry assessed by CASA-Morph and multivariate cluster analysis provides a better comprehension of the ejaculate structure and possibly sperm function. Morphometric analysis provides much more information than data obtained from conventional semen analysis.

关键词： adolescence CASA-Morph system seminal quality sperm head morphometry spermiogram subpopulation

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Tracheal relaxation of five medicinal plants used in Mexico for the treatment of several diseases

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Asian Pacific Journal of Tropical Medicine 2014年第3期7卷 179-183页

作者： Amanda Snchez-Recillas Paul Mantecón-Reyes patricia Castillo-Espaa Rafael Villalobos-molina Maximiliano Ibarra-Barajas Samuel Estrada-Soto Facultad de Farmacia Universidad Autonoma del Estado de MorelosAvenida Universidad 1001.Col.ChamilpaCuernavacaMorelos 62209México Centro de Investigación en Riotecnología Universidad Autónoma del Estado de Morelos.Avenida Universidad 1001Col.ChamilpaCuernavacaMorelos 62209México Unidad de Biomedicina Facultad de Estudios Superiores IztacalaUniversidad Nacional Autónoma de MéxicoTlalnepantlaEstudo de Mexico 54090México

Objective:To assess the relaxant effect of several organic extracts obtained from Agastache mexicana(***),Cochlospermum vitifolium(***),Cordia morelosana(***),Lepechinia caulescens(***)and Talauma mexicana(71 mexicana)used in Mexican traditional medicine for the treatment of several ***:Extracts were obtained by maceration at room temperature using hexane,dicliloromethane and methanol for each plant *** organic extracts were evaluated ex vivo to determine their relaxant activity on the contractions induced by carbaehol(cholinergic receptor agonist,1μmol/L in isolated rat tracheal ***:A total of 15 extracts were evaluated(three for each species).All test samples showed significant relaxant effect,in a concentration-dependent manner,on the contractions induced by 1μmol/L carbachol,with exception of extracts from *** extracts were less potent than theophylline[phosphodiesterase inhibitor,EC_(50):(28.79±0.82)μg/mL]that was used as positive ***-response curves revealed that the extracts with more significant effects were dichloromethanic extracts of ***[E_(max):(103.03±3.32)%and EC_(50):(159.39±3.72)μg/mL)and ***[Emax:(106.58±2.42)%and EC_(50):(219.54±7.61)μg/mL].Finally,hexanic and dichloromethanic extracts from *** were fully effective but less potent than ***,and ***:Less polar extracts obtained from ***,71 mexicana and *** exhibited greater relaxant effect on tracheal rat rings,which allows us to suggest them as sources for the isolation of bioactive molecules with potential therapeutic value in the treatment of asthma.

关键词： Asthma Mexican medicinal plants Trachea Relaxant effect

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Prevalence of type 2 diabetes mellitus in the pediatric population of a third-level care hospital in Mexico City in 2013 and 2018

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World Journal of Diabetes 2023年第3期14卷 290-298页

作者： Jorge Mario molina-Díaz Blanca Estela Vargas-Terrez patricia Guadalupe Medina-Bravo Antonia Martínez-Ambrosio América Liliana Miranda-Lora Miguel Klünder-Klünder Department of Child Endocrinology Children’s Hospital Federico GomezAutonomous University of MexicoMexico City 06250Mexico Faculty of Business La Salle UniversityMexico City 06250Mexico Department of Endocrinology Children’s Hospital Federico GomezMexico City 06720Mexico Department of Nursing and Endocrinology Children’s Hospital Federico GómezMexico City 06720Mexico Epidemiological Research Unit in Endocrinology and Nutrition Children’s Hospital Federico GomezMexico City 06720Mexico Division of Research Children’s Hospital Federico GomezMexico City 06720Mexico

BACKGROUND Diabetes mellitus type 2(T2DM)is a state of hyperglycemia due to a defect in the secretion and/or action of insulin,and it represents the most common form of diabetes *** Mexico,10.3%of the adult population have been diagnosed with T2DM and it is expanding to the pediatric *** To evaluate and compare the prevalence of T2DM in the pediatric population at the Children’s Hospital,“Hospital Infantil de México Federico Gómez(HIMFG)”,at two time periods:2013 vs *** A comparison of two cross-sectional studies was done(2013 and 2018).The study population was comprised of children and adolescents 8-17 years old,from the Diabetes Clinic at the aforementioned institution.A comprehensive interrogation regarding family history and perinatal antecedents was *** blood work after 12 h of fasting was obtained to determine serum levels of glucose,glycated hemoglobin,lipid profile,C-peptide,and *** data were analysed using the statistical software package SPSS v.23.0.A P value of<0.05 was considered statistically *** A total of 151 patients were included:47 from 2013,and 104 from *** were age differences noted between the two periods with younger patients presenting T2DM in ***,T2DM predominated in the male sex in 55.36%in 2013 vs 32.7%in *** increased prevalence of T2DM was noted from 2013 to 2018(20.2%vs 33.0%,respectively),which was a statistically significant 12.8%increase(P<0.0001).The illness phenotype was more aggressive in the 2018 group with the presence of a higher proportion of obesity,insulin resistance,and adverse lipid *** The prevalence of T2DM at the HIMFG institution from 2013 to 2018 increased by 12.8%(20.2%vs 33.0%,respectively).The study results demonstrate the need for vigilance in T2DM trends,and to strengthen programs of healthy nutrition and physical activity as well as early detection and risk factors for obesity,data on insulin resistance,and metabo

关键词： Diabetes Children adolescents Epidemiology Etiology Risk factors and complications

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Clinical-pathological and molecular characterization of long-term survivors with advanced non-small cell lung cancer

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Cancer Biology & Medicine 2020年第2期17卷 444-457页

作者： Juan Moreno-Rubio Santiago Ponce Rosa Alvarez Maria Eugenia Olmedo Sandra Falagan Xabier Mielgo Fatima Navarro patricia Cruz Luis Cabezon-Gutierrez Carlos Aguado Gonzalo Colmenarejo Marta Munoz-Fernandez de Leglaria Ana Belen Enguita Maria Cebollero Amparo Benito Isabel Alemany Carolina del Castillo Ricardo Ramos Ana Ramirez de molina Enrique Casado Maria Sereno Ana Ramirez de molina1 Enrique Casado5Maria Sereno5 XIMDEA Food InstituteCEI UAM+CSICMadrid 28702Spain 12 de Octubre University Hospital Madrid 28041Spain Gregorio Maranon University Hospital Madrid 28009Spain Ramon y Cajal University Hospital Madrid 28034Spain Infanta Sofia University Hospital San Sebastian De Los ReyesMadrid 28702Spain Fundacion Alcorcon University Hospital AlcorconMadrid 28922Spain Prlncipe de Asturias University Hospital Alcala De HenaresMadrid 28805Spain La Paz University Hospital Madrid 28046Spain University Hospital of Torrejon Torrejon De ArdozMadrid 28850Spain Clinico San Carlos University Hospital Madrid 28040Spain Parque Cientifico de Madrid Foundation Madrid 28049Spain

Objective:Long-term survivors(LS)of non-small cell lung cancer(NSCLC)without driver alterations,displaying an overall survival(OS)of more than 3 years,comprise around 10%of cases in several series treated with *** are classical prognosis factors for these cases[stage,Eastern Cooperative Oncology Group(ECOG),etc.],but more data are required in the *** this multi-center study,we focused on LS of advanced NSCLC with OS above 36 months to perform a clinical-pathological and molecular ***:In the first step,we conducted a clinical-pathological characterization of the ***,we carried out a genetic analysis by comparing LS to a sample of short-term survivors(SS)(with an OS less than 9 months).We initially used whole-genome RNA-seq to identify differentiating profiles of LS and SS,and later confirmed these with reverse transcription-polymerase chain reaction(RT-PCR)for the rest of the ***:A total of 94 patients were included,who were mainly men,former smokers,having adenocarcinoma(AC)-type NSCLC with an ECOG of *** obtained an initial differential transcriptome expression,displaying 5 over-and 33 under-expressed genes involved in different pathways:namely,the secretin receptor,surfactant protein,trefoil factor 1(T FF1),serpin,Ca-channels,and Tolllike receptor(TLRs)***,RT-PCR analysis of 40(20 LS/20 SS)samples confirmed that four genes(surfactant proteins and SFTP)were significantly down-regulated in SS compared to LS by using an analysis of covariance(ANCOVA)model:SFTPA1(P=0.023),SFTPA2(P=0.027),SFTPB{P=0.02),and SFT PC(P=0.047).Conclusions:We present a sequential genetic analysis of a sample of NSCLCLS with no driver alterations,obtaining a differential RNA-seq/RT-PCR profile showing an abnormal expression of SF genes.

关键词： Long-term survivors non-small cell lung cancer surfactant proteins

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Untargeted metabolomics to evaluate antifungal mechanism:a study of Cophinforma mamane and Candida albicans interaction

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Natural Products and Bioprospecting 2023年第1期13卷 779-787页

作者： Asih Triastuti Marieke Vansteelandt Fatima Barakat Carlos Amasifuen patricia Jargeat Mohamed Haddad UMR 152 Pharma Dev IRDUPSUniversitéde Toulouse31400 ToulouseFrance Department of Pharmacy Universitas Islam IndonesiaYogyakarta 55584Indonesia Dirección de Recursos Genéticos y Biotecnología Instituto Nacional de Innovación AgrariaAvenida La Molina 1981La MolinaLima 15024Peru Laboratoire Evolution et DiversitéBiologique UMR 5174 CNRSIRDUPSUniversitéde Toulouse31062 ToulouseFrance

Microbial interactions between filamentous fungi and yeast are still not fully *** evaluate a potential anti-fungal activity of a filamentous fungus while highlighting metabolomic changes,co-cultures between an endophytic strain of Cophinforma mamane(CM)and Candida albicans(CA)were *** liquid cultures were incubated under static conditions and metabolite alterations during the course were investigated by ultra-performance liquid chromatography-tandem mass spectrophotometry(UPLC-MS/MS).Results were analyzed using MS-DIAL,MS-FINDER,METLIN,Xcalibur,SciFinder,and MetaboAnalyst metabolomics *** metabolites associated with catabolic processes,including the metabolism of branched-chain amino acids,carnitine,and phospholipids were upregulated both in the mono and co-cultures,indicating fungal adaptability to environmental *** metab-olites,including C20 sphinganine 1-phosphate,myo-inositol,farnesol,gamma-undecalactone,folinic acid,palmitoleic acid,and MG(12:/0:0/0:0)were not produced by CA during co-culture with CM,demonstrating the antifungal mecha-nism of *** results highlight the crucial roles of metabolomics studies to provide essential information regarding the antifungal mechanism of *** against ***,especially when the lost/undetected metabolites are involved in fungal survival and pathogenicity.

关键词： Metabolomics Fungal co-culture Anti-fungal Virulence

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Problem-Based Learning <i>in Situ</i>Applied to Students in the Assessment of Nursing Process

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Open Journal of Nursing 2021年第5期11卷 378-389页

作者： Margarita Acevedo-Peñ a Rosa María Ostiguín-Meléndez José Luis Cadena-Anguiano Marcela patricia Ibarra-Gonzalez Jesús Rigoberto Hernández-Hernández Rafael Villalobos-molina Diana Cecilia Tapia-Pancardo Facultad de Estudios Superiores Iztacala Universidad Nacional Autónoma de México Mexico City Mexico Escuela Nacional de Enfermería y Obstetricia Universidad Nacional Autónoma de México Mexico City Mexico Facultad de Estudios Superiores Iztacala Universidad Nacional Autónoma de México Mexico City Mexico Colegio de Posgrado de la Ciudad de México city Mexico Unidad de Biomedicina Facultad de Estudios Superiores Iztacala Universidad Nacional Autónoma de México Mexico City Mexico

Background: Nursing is considered a science in the making that recognizes the nursing process (NP) as the scientific and methodological tool to provide care. The NP is composed of five stages of which the assessment is the first and fundamental to identify the health problems of the people. This research aimed to evaluate a pedagogical didactic strategy that is based on situated constructivism, the Based-Problem Learning (BPL), in order to improve significant learning. Methods: Quantitative research with an explanatory scope, was transversal, and it was applied to 30 students of the first year of the nursing career at the Faculty of Higher Studies Iztacala, National Autonomous University of Mexico. The sample was chosen considering the inclusion criteria. Data were collected using a Likert-type instrument validated in a previous investigation. Results: The average value obtained from the Likert scale to evaluate the intervention was 103.4, the highest score was 135 and the lowest was 83, which reflects that it was evaluated as good and particularly good. The grade given to the teacher as an agent of the ZPD, was between agree and totally agree, the highest percentages scored at 93%, since it provoked challenge in their thinking, and favored interaction among classmates. Conclusions: The application of the in situ teaching strategy, BPL favored significant learning of the assessment stage, the students were able to carry out the assessment in the hospital, the experience contributed to generate significant learning when students face real scenarios, which showed the effectiveness of the method.

关键词： Learning Method Nursing Process Assessment

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Microstructure of <em>Annona muricata</em>L. Leaves Extract Microcapsules Linked to Physical and Chemical Characteristics

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Journal of Encapsulation and Adsorption Sciences 2018年第3期8卷 178-193页

作者： Oscar Jordán-Suárez patricia Glorio-Paulet Leslie Vidal Facultad de Ciencia de Alimentos Universidad Le Cordon Bleu Lima Perú Departamento de Ingeniería de Alimentos y Productos Agropecuarios Universidad Nacional Agraria La Molina Lima Perú Departamento de Agroindustrias Universidad de Concepción Chillán Chile

Microstructure is closely related to techno-functional properties in microencapsulated powders intended to protect bioactive compounds. Soursop leaves provide phytochemicals that need to be protected to ensure their functionality. This investigation aimed to study the microstructure of microcapsules containing soursop leaves extract and its linkage with physical and chemical characteristics of the resulting powder. Microcapsules were prepared by spray drying using gum Arabic and maltodextrin as encapsulating agents at 5 and 10%. Powders were characterized by scanning electron microscopy, particle size analysis, solubility, infrared spectroscopy and encapsulation efficiency. Microphotographs showed spherical shape particles ranging from 0.25 to 13.87 μm, where the particles morphology depended on the concentration and the type of the encapsulant used. At higher concentration of encapsulant, there was an increase in the sphericity, integrity, size, and surface smoothness of particles. This relationship was inverse for solubility in treatments with gum Arabic. The extract encapsulation was confirmed by Fourier Transform infrared spectroscopy and encapsulation efficiency index, revealing that the treatment with maltodextrin at 10% showed a better capability for entrapment (72.12%). The results evidence that microstructure of microcapsules is closely linked to the type and concentration of encapsulant, which in turn determine the physical and chemical characteristics of powders intended for instant drinks solubility and entrapping soursop bioactive compounds.

关键词： Soursop Microencapsulation Microstructure Spray Drying Encapsulants

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