检索结果-南通市图书馆

Characterization of Evolutionarily ConservedMotifs Involved in Activity and Regulation of theABA-INSENSITIVE （ABI） 4 Transcription Factor

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Plant 2014年第2期7卷 422-436页

作者： Josefat Gregorio Alma Fabiola hernández-Bernal Elizabeth Cordoba patricia León Departamento de Biología Molecular de Plantas Instituto de Biotecnología Universidad Nacional Autónoma de México Av. Universidad # 2001 Col. Chamilpa Cuernavaca Morelos C.p. 62210 Mexico

In recent years, the transcription factor ABI4 has emerged as an important node of integration for externaland internal signals such as nutrient status and hormone signaling that modulates critical transitions during the growthand development of plants. For this reason, understanding the mechanism of action and regulation of this protein rep-resents an important step towards the elucidation of crosstalk mechanisms in plants. However, this understanding hasbeen hindered due to the negligible levels of this protein as a result of multiple posttranscriptional regulations. To betterunderstand the function and regulation of the ABI4 protein in this work, we performed a functional analysis of severalevolutionarily conserved motifs. Based on these conserved motifs, we identified ortholog genes of ABI4 in differentplant species. The functionality of the putative ortholog from Theobroma cacao was demonstrated in transient expres-sion assays and in complementation studies in plants. The function of the highly conserved motifs was analyzed aftertheir deletion or mutagenesis in the Arabidopsis ABI4 sequence using mesophyll protoplasts. This approach permitted usto immunologically detect the ABI4 protein and identify some of the mechanisms involved in its regulation. We identi-fied sequences required for the nuclear localization （AP2-associated motif） as well as those for transcriptional activationfunction （LRP motif）. Moreover, this approach showed that the protein stability of this transcription factor is controlledthrough protein degradation and subcellular localization and involves the AP2-associated and the PEST motifs. We dem-onstrated that the degradation of ABI4 protein through the PEST motif is mediated by the 26S proteasome in responseto changes in the sugar levels.

关键词： ABI4 posttranslational regulation transcription factor sugar signaling abscisic acid-insensitive protea-some nuclear localization signals

Three-dimensional models:from cell culture to Patient-Derived Organoid and its application to future liposarcoma research

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Oncology Research 2025年第1期33卷 1-13页

作者： SAYUMI TAHARA SYDNEY RENTSCH FERNANDA COSTAS CASAL DE FARIA patricia SARCHET ROMA KARNA FEDERICA CALORE RAPHAEL E.POLLOCK Department of Surgery Division of Surgical OncologyThe James Comprehensive Cancer CenterThe Ohio State University Wexner Medical CenterColumbusOH 43210USA Department of Cancer Biology and Genetics The James Comprehensive Cancer CenterThe Ohio State University Wexner Medical CenterColumbusOH 43210USA

Liposarcoma is one of the most common soft tissue sarcomas,however,its occurrence rate is still rare compared to other *** to its rarity,in vitro experiments are an essential approach to elucidate liposarcoma *** cell culture-based research(2D cell culture)is still playing a pivotal role,while several shortcomings have been recently under *** vivo,mouse models are usually adopted for pre-clinical analyses with expectations to overcome the issues of 2D cell ***,they do not fully recapitulate human dedifferentiated liposarcoma(DDLPS)***,three-dimensional(3D)culture systems have been the recent research focus in the cell biology field with the expectation to overcome at the same time the disadvantages of 2D cell culture and in vivo animal models and fill in the gap between *** the liposarcoma rarity,we believe that 3D cell culture techniques,including 3D cell cultures/co-cultures,and Patient-Derived tumor Organoids(PDOs),represent a promising approach to facilitate liposarcoma investigation and elucidate its molecular mechanisms and effective therapy *** this review,we first provide a general overview of 3D cell cultures compared to 2D cell *** then focus on one of the recent 3D cell culture applications,Patient-Derived Organoids(PDOs),summarizing and discussing several PDO ***,we discuss the current and future applications of PDOs to sarcoma,particularly in the field of liposarcoma.

关键词： Cell culture Liposarcoma Patient-Derived Organoid(PDO) Spheroid Three-dimensional(3D)cell culture

Mechanisms of retinal neuroprotection of calcium dobesilate：therapeutic implications

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2017年第10期12卷 1620-1622页

作者： Olga Simó-Servat Cristina Solà-Adell patricia Bogdanov Cristina hernández Rafael Simó Diabetes and Metabolism Research Unit Vall d'Hebron Research InstituteCIBERDEM(Instituto de Salud CarlosⅢ)BarcelonaSpain

Current treatments for diabetic retinopathy （DR） are based on laser photocoagulation and intravitreal injections of corti- costeroids or anti-vascular endothelial growth factor （VEGF） agents. These treatments are applicable only at advanced stages of the disease. In addition, they are expensive, require a vitreo- retinal specialist and are associated with significant adverse ef- fects. Therefore, new pharmacological treatments for the early stages of the disease are needed.

关键词： Ca Mechanisms of retinal neuroprotection of calcium dobesilate:therapeutic implications

Host-parasite interactions in perpetual darkness:Macroparasite diversity in the cavefish Astyanax mexicanus

在线全文

学校读者我要写书评

暂无评论

Zoological Research 2023年第4期44卷 782-792页

作者： Ana Santacruz David hernández-Mena Ramses Miranda-Gamboa Gerardo Pérez-Ponce De León Claudia patricia Ornelas-García Departamento de Zoología Instituto de BiologíaUniversidad Nacional Autónoma de MéxicoMéxico City CP 04510México Departamento de Sistemas y Procesos Naturales Escuela Nacional de Estudios Superiores Unidad MéridaUcúYucatán CP 97357México Instituto de Energías Renovables Universidad Nacional Autónoma de MéxicoTemixcoMorelos CP 62580México Colección Nacional de Peces Departamento de ZoologíaInstituto de BiologíaUniversidad Nacional Autónoma de MéxicoMéxico City CP 04510México Stowers Institute for Medical Research Kansas CityMO 64110USA

Astyanax mexicanus has repeatedly colonized cave environments,displaying evolutionary parallelisms in many troglobitic *** being a model system for the study of adaptation to life in perpetual darkness,the parasites that infect cavefish are practically *** this study,we investigated the macroparasite communities in 18 cavefish populations from independent lineages and compared them with the parasite diversity found in their sister surface fish populations,with the aim of better understanding the role that parasites play in the colonization of new *** the cavefish populations,we identified 13 parasite taxa,including a subset of 10 of the 27 parasite taxa known for the surface *** infecting the cavefish belong to five taxonomic groups,including trematodes,monogeneans,nematodes,copepods,and *** are the most dominant group,found in 14 *** macroparasites include species with direct life cycles and trophic transmission,including invasive ***,paired comparisons indicate higher parasite richness in the cavefish than in the surface *** variation in parasite composition across the caves suggests historical and geographical contingencies in the host-parasite colonization process and potential evolution of local *** base-line data on parasite diversity in cavefish populations of *** provides a foundation to explore the role of divergent parasite infections under contrasting ecological pressures(cave *** environments)in the evolution of cave adaptive traits.

关键词： Adaptation Metazoan parasites Niche change Parasite assemblage Prevalence

Predicting Standardized Precipitation Evapotranspiration Index for Fada N’Gourma by 2050 Using CMPI-6 Outputs under SSPs Scenarios

在线全文

学校读者我要写书评

暂无评论

Journal of Environmental Science and Engineering(A) 2025年第1期14卷 31-42页

作者： Tog-Noma patricia Emma Bontogho Andrew Ireson Dominic Boateng-Gyambiby Abdullahi Dini High Institute of Sustainable Development University Yembila Abdoulaye ToguyéniBP 54 Fada N’GourmaBurkina Faso Global Institute for Water Security School of Environment and SustainabilityUniversity of SaskatchewanSaskatoonCanada Department of Agricultural and Resource Economics University of Energy and Natural Resourcescity P.O.Box 214SunyaniGhana

Extreme weather events,such as floods and droughts,are expected to rise significantly worldwide as a result of climate *** future drought patterns is therefore a key approach for elaborating anticipatory water resources management responses to climate *** this paper,future meteorological drought conditions are investigated based on the SPEI(Standardised Precipitation Evapotranspiration Index).This study makes use of observed and projected *** simulated data were retrieved from the CMIP6(Coupled Model Intercomparison Project Phase 6)over the period 2025-2050,and the Delta change method was adopted to remove the bias in the *** SPEI at various scales has been estimated under four future scenarios(SSP1-2.6,SSP2-4.5,SSP3-7.0 and SSP5-8.5).The trend analysis of the projected SPEI was performed at p<0.05 using the MMK(Modified Mann-Kendall)test in order to detect the statistically significant trend of the drought against the null hypothesis of no *** show large variability in the magnitude of drought in the past and *** on SPEI at 24 months accumulation,the result shows that under SSP1-2.6,the basin will experience a wet period during the first decade(SPEI=0.60),the second decade will be dry(SPEI24=-0.43).The remaining years will be also dry(SPEI=-0.34).Under SSP2-4.5,SSP3-7.0 and SSP5-8.5 scenarios,the district will experience a wet period during the first two decades with SPEI ranging from 0.38 to *** wet period will be followed by a dry period under these scenarios ranging from-0.14 ***,under SSPs scenarios,two main periods characterized by a rainfall recovery spanning from followed by a moderately prolonged drought are identified within the study *** findings of this study may provide valuable information for developing proactive measures to reduce water insecurity in Fada N’Gourma through effective drought mitigation.

关键词： Climate change drought index CMPI-6 Fada N’Gourma SSP-scenarios.

Role of lipids in the control of autophagy and primary cilium signaling in neurons

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2024年第2期19卷 264-271页

作者： María Paz hernández-Cáceres Daniela Pinto-Nuñez patricia Rivera Paulina Burgos Francisco Díaz-Castro Alfredo Criollo Maria Jose Yañez Eugenia Morselli Instituto de Investigación en Ciencias Odontológicas(ICOD) Facultad de OdontologíaUniversidad de ChileSantiagoChile Department of Basic Sciences Faculty of Medicine and ScienceUniversidad San SebastiánSantiagoChile Physiology Department Faculty of Biological SciencesPontificia Universidad Católica de ChileSantiagoChile Advanced Center for Chronic Diseases(ACCDiS) Facultad de Ciencias Químicas y Farmacéuticas&Facultad de MedicinaUniversidad de ChileSantiagoChile Autophagy Research Center SantiagoChile

The brain is,after the adipose tissue,the organ with the greatest amount of lipids and diversity in their composition in the human *** neurons,lipids are involved in signaling pathways controlling autophagy,a lysosome-dependent catabolic process essential for the maintenance of neuronal homeostasis and the function of the primary cilium,a cellular antenna that acts as a communication hub that transfers extracellular signals into intracellular responses required for neurogenesis and brain development.A crosstalk between primary cilia and autophagy has been established;however,its role in the control of neuronal activity and homeostasis is barely *** this review,we briefly discuss the current knowledge regarding the role of autophagy and the primary cilium in *** we review the recent literature about specific lipid subclasses in the regulation of autophagy,in the control of primary cilium structure and its dependent cellular signaling in physiological and pathological conditions,specifically focusing on neurons,an area of research that could have major implications in neurodevelopment,energy homeostasis,and neurodegeneration.

关键词： autophagic flux cholesterol fatty acids GPCR lysosomal storage diseases neurons NPC1 phosphoinositides primary cilium

Autoantibodies against beta cells to predict early insulin requirements in pediatric patients with clinically diagnosed type 2 diabetes

在线全文

学校读者我要写书评

暂无评论

World Journal of Diabetes 2024年第8期15卷 1717-1725页

作者： Jorge M Molina patricia G Medina Rita A Gomez Julia R Herrera Nancy L Martínez Brenda hernández Yesenia García Department of Endocrinology Children’s Hospital Federico GomezMexico 06720Mexico National Medical Center"Siglo XXI" UMAE Hosp EspecialidadesUnidad Invest Med Epidemiol ClinMexican Social Security InstituteMexico 06720Mexico Research Division UMAE Hospital de EspecialidadesCentro Médico Nacional Siglo XXIMexican Institute of Social SecurityMexico 06720Mexico Epidemiology Research Unit in Endocrinology and Nutrition Federico Gómez Children’s HospitalMexico 06720Mexico Department of Endocrinology Comprehensive Health Unit for Trans PersonsMexico 11340Mexico

BACKGROUND Autoimmunity has emerged as a probable disease modifier in patients with clinically diagnosed type 2 diabetes mellitus(T2DM),that is,patients who have insulin resistance,obesity,and other cardiovascular risk factors,suggesting that the presence of glutamic acid decarboxylase(anti-GAD65),islet antigen 2(anti-IA2),and zinc transporter 8(anti-Zn8T)antibodies could have deleterious effects on beta cell function,causing failure and earlier requirement for insulin *** To evaluate anti-GAD65,anti-IA2 and anti-Zn8T as predictors of early insulin requirement in adolescents with a clinical diagnosis of *** This was a case–control study in patients with clinically diagnosed with T2DM(68 cases and 64 controls with and without early insulin dependence respectively),male and female,aged 12–18 ***,blood pressure,glucose,insulin,C-peptide,glycated hemoglobin A1c,and lipid profiles were *** was used to measure anti-GAD65,anti-IA2,and anti-Zn8T *** statistics,Pearson'sχ2 test,Student's t test,and logistic regression was performed.P<0.05 was considered statistically *** There were 132 patients(53.8%female),with a mean age was 15.9±1.3 years,and there was a disease evolution time of 4.49±0.88 *** presence of anti-GAD65,anti-IA2,and anti-Zn8T positivity was found in 29.5%,18.2%,and 15.9%,*** the groups by early or no insulin dependence showed that the group with insulin had a higher frequency of antibody positivity:anti-GAD65 odds ratio(OR):2.42(1.112–5.303,P=0.026);anti-IA2:OR:1.55(0.859–2.818,P=0.105);and anti-Zn8T:OR:7.32(2.039–26.279,P=0.002).CONCLUSION Anti-GAD65 positivity was high in our ***-GAD65 and anti-Zn8T positivity showed a significantly depleted beta cell reserve phenotype,leading to an increased risk of early insulin dependence.

关键词： Diabetes Obesity Adolescents Autoimmunity Beta cell Insulin requirements

Treating amyotrophic lateral sclerosis with allogeneic Schwann cell-derived exosomal vesicles: a case report

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第4期20卷 1207-1216页

作者： Pascal J.Goldschmidt-Clermont Aisha Khan George Jimsheleishvili patricia Graham Adriana Brooks Risset Silvera Alexander J.P.Goldschmidt Damien D.Pearse W.Dalton Dietrich Allan D.Levi James D.Guest Miller School of Medicine University of MiamiMiamiFLUSA Stem Cell Institute Miller School of MedicineUniversity of MiamiMiamiFLUSA The Miami Project to Cure Paralysis Miller School of MedicineUniversity of MiamiMiamiFLUSA Alzady International LLC MiamiFLUSA Neurological Surgery Miller School of MedicineUniversity of MiamiMiamiFLUSA

Schwann cells are essential for the maintenance and function of motor neurons,axonal networks,and the neuromuscular *** amyotrophic lateral sclerosis,where motor neuron function is progressively lost,Schwann cell function may also be ***,important signaling and potential trophic activities of Schwann cell-derived exosomal vesicles have been *** case report describes the treatment of a patient with advanced amyotrophic lateral sclerosis using serial intravenous infusions of allogeneic Schwann cell-derived exosomal vesicles,marking,to our knowledge,the first instance of such *** 81-year-old male patient presented with a 1.5-year history of rapidly progressive amyotrophic lateral *** initial diagnosis,the patient underwent a combination of generic riluzole,sodium phenylbutyrate for the treatment of amyotrophic lateral sclerosis,and *** patient volunteered to participate in an FDA-approved single-patient expanded access treatment and received weekly intravenous infusions of allogeneic Schwann cell-derived exosomal vesicles to potentially restore impaired Schwann cell and motor neuron *** confirmed that cultured Schwann cells obtained from the amyotrophic lateral sclerosis patient via sural nerve biopsy appeared impaired(senescent)and that exposure of the patient’s Schwann cells to allogeneic Schwann cell-derived exosomal vesicles,cultured expanded from a cadaver donor improved their growth capacity in *** a period of observation lasting 10 weeks,during which amyotrophic lateral sclerosis Functional Rating Scale-Revised and pulmonary function were regularly monitored,the patient received weekly consecutive infusions of 1.54×1012(×2),and then consecutive infusions of 7.5×1012(×6)allogeneic Schwann cell-derived exosomal vesicles diluted in 40 mL of Dulbecco’s phosphate-buffered *** of the infusions were associated with adverse events such as infusion reactions(allergic or otherwise)or

关键词： allogeneic amyotrophic lateral sclerosis exosomes infusion neuromuscular junction Schwann cell

Principal Component Analysis of Male Criminal Behavior

在线全文

学校读者我要写书评

暂无评论

Health 2021年第10期13卷 1112-1128页

作者： patricia Martínez-Lanz Carlos Cuevas-Covarrubias patricia hernández-Valdez Interdisciplinary Research Center Universidad Anáhuac México Mexico City Mexico Research Center of Statistics and Applied Mathematics Universidad Anáhuac México Mexico City Mexico

The present study was conducted in order to establish factors that can potentially facilitate crime, as well as the status of the emotional wellbeing presented in the prison population. The sample was composed of 358 inmates of the Federal Center for Social Rehabilitation number 7 in Mexico. A questionnaire was specifically developed;it evaluated sociodemographic factors and Likert scales of substance intake, domestic violence, and depressive symptoms. Validity and reliability (Cronbach’s Alpha = 0.703) of the instrument showed appropriate relations between the reagents of the scales;results showed—through Chi-Square analysis—statistically significant differences in the correlations between sociodemographic factors, domestic violence, addictions, and depressive symptoms. Although results showed a connection between domestic violence and substance abuse with criminal behavior, low socioeconomic conditions exhibited a higher degree of correlation with criminal activity. On the other hand, high depression symptoms are present in one out of every five inmates.

关键词： Delinquency Addictions Violence Depression Crime Criminal Behavior Prison Population Wellbeing