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Using pipette tips to readily generate spheroids comprising single or multiple cell types

Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2023年第10期24卷 875-885页

作者： Rong pan Xiaoyan YANG Shiming WU yuanyuan XIE Feng CHEN ke NING Wei SUN Ling yu key Laboratory of Luminescence Analysis and Molecular Sensing Ministry of EducationInstitute for Clean Energy and Advanced MaterialsSchool of Materials and EnergySouthwest UniversityChongqing 400715China College of Chemistry and Chemical Engineering Hainan Normal UniversityHaikou 571158China

Three-dimensional(3D)cell culture methods have been validated that can replicate the tumor environment in vivo to a large extent,providing an effective tool for studying *** this study,we demonstrated the use of standard laboratory pipette tips as micro vessels for generating 3D cell *** microfabrication or wet-chemistry surface modifications were involved in the *** consisting of single or multiple cell types were generated within 24 h just by pipetting and incubating a cell suspension in pipette *** electron microscope and optical microscope proved that the cells grew together tightly,and suggested that while gravity force might have initiated the sedimentation of cells at the bottom of the tip,the active aggregation of cells to form tight cell-cell interactions drove the formation of *** common laboratory micropipettes and pipette tips,the rate of spheroid generation and the generation reproducibility was characterized from five boxes each with 80 *** ease of transferring reagents allowed modeling of the growth of microvascular endothelial cells in tumor ***,the pairing and fusion of tumor spheroids could be manipulated in the pipette tips,suggesting the potential for building and assembling heterogeneous micro-tumor tissues in vitro to mimic solid tumors in *** study demonstrated that spheroids can be readily and cost-effectively generated in standard biological laboratories in a timely manner using pipette tips.

关键词： Pipette tip 3D cell culture Tumor spheroids Co-culture In-situ observation

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A Prediction Method of Internal Solitary Wave Loads on the Semi-Submersible Platform

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China Ocean Engineering 2022年第3期36卷 464-473页

作者： ZHANG Jing-jing CHEN ke YOU yun-xiang HAN pan-pan State key Laboratory of Ocean Engineering Shanghai Jiao Tong UniversityShanghai 200240China School of Naval Architecture Ocean and Civil EngineeringShanghai Jiao Tong UniversityShanghai 200240China

An investigation into the prediction method for internal solitary waves(ISWs)loads on the columns and caissons of the semi-submersible platform found on three kinds of internal solitary wave theories and the modified Morison Equation is *** characteristics of loads exerted on the semi-submersible platform model caused by the ISWs have been observed experimentally,and the inertial and drag coefficients in Morison Equation are determined by analyzing the forces of *** the results,it is of interest to find that Reynolds number,KC number and layer thickness ratio have a considerable influence on the *** direction of incoming waves,how-ever,is almost devoid of effects on the *** drag coefficient of columns varies as an exponential function of Reynolds number,and inertia coefficient of columns is a power function related to KC ***,the drag coefficient of caissons is approximately constant in terms of regression analysis of experimental *** results from different experimental conditions reveal that the inertia coefficient of caissons appears to be exponential correlated with upper layer depths.

关键词： semi-submersible platform Morison equation model test ISWs coefficients

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New technological partition for “three zones” spontaneous coal combustion in goaf

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International Journal of Mining Science and Technology 2013年第4期23卷 487-491页

作者： pan Rongkun Cheng yuanping yu Minggao Lu Chang Yang ke Department of Safety & Science Engineering Henan Polytechnic University National Engineering Research Center of Coal Gas Control Faculty of Safety Engineering China University of Mining & Technology College of Resource and Environmental Science Chongqing University

On detailed analysis basis of spontaneous coal combustion for the three zones in mine goaf,we use O2 and CO concentrations to divide the three zones of the coal *** our experiment,we selected a typical working face and focused on the changes in gas *** order to overcome establishment limitations of actual layout location and underground conditions in a mine goaf,we based our observations on the three zones,combined them with numerical simulation,described the distribution and the changes in O2 and CO concentrations during the coal spontaneous combustion in the goaf,which provided us with an understanding of the distribution of coal spontaneous combustion in the three zones in the form of ***,our study summarizes the changes of O2and CO concentrations in the entire goaf and shows them to be in agreement with our observations at the *** study shows that it is feasible to divide the three zones,given our comprehensive targets of O2,CO and our numerical *** method avoids the limitation of dividing the three zones with a single target and the likely errors of observations at the *** addition,the method offers a basis for optimizing measures of fre-fghting with important and practical effects.

关键词： Goaf Spontaneous combustion Three zones Target

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Sorption kinetics of parent and substituted PAHs for low-density polyethylene（LDPE）:Determining their partition coefficients between LDPE and water(KLDPE) for passive sampling

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Journal of Environmental Sciences 2020年第1期32卷 349-360页

作者： Pei Lei Jinjie Zhu ke pan Hong Zhang State key Laboratory of Pollution Control and Resources Reuse School of the EnvironmentNanjing UniversityNanjing 210023China State key Laboratory cf Environmental Aquatic Chemistry Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China Institute for Advanced Study Shenzhen UniversityShenzhen 518060China University of Chinese Academy of Sciences Beijing 100049China

Low-density polyethylene(LDPE)has been widely used as a sorbent for passive sampling of hydrophobic organic contaminants(HOCs)in aquatic ***,it has seen only limited application in passive sampling for measurement of freely dissolved concentrations of parent and substituted PAHs(SPAHs),which are known to be toxic,mutagenic and ***,the 16 priority PAHs and some typical PAHs were selected as target compounds and were simultaneously determined by gas chromatography–mass spectrometer(GC–MS).Some batch experiments were conducted in the laboratory to explore the adsorption kinetics of the target compounds in LDPE *** results showed that both PAHs and SPAHs could reach equilibrium status within19–38 days in sorption kinetic *** coefficients of partitioning between LDPE film(50μm thickness)and water(KLDPE)for the 16 priority PAHs were in good agreement with previously reported values,and the values of KLDPEfor the 9 SPAHs are reported in this study for the first *** linear relationships were observed,i.e.,log KLDPE=0.705×log KOW+1.534 for PAHs(R^2=0.8361,p<0.001)and log KLDPE=0.458×log KOW+3.092 for SPAHs(R^2=0.5609,p=0.0077).The selected LDPE film was also proven to meet the condition of"zero sink"for the selected target *** results could provide basic support for the configuration and in situ application of passive samplers.

关键词： Low-density polyethylene(LDPE) PAHs Substituted PAHs(SPAHs) Freely dissolved concentrations Passive sampling

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Magnetic performance and corrosion resistance of electroless plating CoWP film

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Rare Metals 2012年第3期31卷 264-271页

作者： Jiang, Li pan, Shanshan Lu, Jianbiao ke, Xin He, Wenhao yu, yundan Wei, Guoying Ge, Hongliang College of Materials Science and Engineering China Jiliang University Hangzhou 310018 China Zhejiang keda Magnetoelectricity Limited Company Huzhou 313200 China

The CoWP film with good magnetic performance and corrosion resistance was electrolessly plated from alkaline solution. The technical parameters of the electroless plating system were optimized. When the pH value of electroless plating solution was 11.0 and the reducing agent (NaH2PO2) content was 0.4 mol L 1, the target chemical reactions proceeded in the electroless plating solution smoothly with negligible interference and side effects. CoWP film prepared under optimal deposition condition contained more hexagonal close-packed (hcp) cobalt (ε-Co) of [110] and crystallographic orientation of Co [002]. VSM analysis reveals that the saturation magnetization of the CoWP film is 100～220 Am2 kg 1 and coercivity is (2.87～4.38)×104 A·cm 1. The corrosion behavior of CoWP film in 3.5% NaCl aqueous solution was studied by electrochemical experiments. The results prove that the corrosion resistance of the CoWP film deposited under the optimal depo-sition condition exhibites relatively lower corrosion current of (3.054～3.162)×10 6 A·cm 2. The surface morphology analysis indicates that the film is smooth and composed of regular-shaped crystallites.

关键词： CoWP film corrosion magnetic microstructure

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Magnetic and microwave absorbing properties of Ce-Co-based alloy powders driven with lanthanum content

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Journal of Rare Earths 2020年第7期38卷 749-754,I0003页

作者： yu He Shunkang pan Jingjing yu School of Material Science and Engineering Guilin University of Electronic TechnologyGuilin 541004China

The composites of Ce-Co-based alloys doped with La content were fabricated via a vacuum arc melting *** influences of La addition on microstructure,electromagnetic parameters,magnetic property and microwave absorbing property were measured by the corresponding *** morphology characteristics manifest that all samples display sheet structure,and the average particle size of alloy powders increases with increasing La content The saturation magnetization(MS) decreases with increasing La addition as a *** minimum reflection loss(RL) of La0.4Ce1.6Co17 alloy powder about-42.29 dB can be obtained about-42.29 dB at 7.84 GHz with the matching thickness of 1.8 mm,and the corresponding effective bandwidth can achieve about 2.24 *** addition,the minimum RL frequency moves towards a lower frequency region as the La content *** minimum RL of La0.3Ce1.7Co17alloy powder is less than-20 dB ranging from 1.2 to 2.4 mm in the whole 4-16 *** maximum bandwidth can reach about 4.88 GHz at the given thickness of 1.2 *** general,these all indicate that the La addition is beneficial to improving the microwave absorbing performance in both effective bandwidth and absorption intensity.

关键词： Ce-Co-Based alloys Magnetic property Microwave absorbing property Reflection loss Rare earths

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Clustering and multi-hop routing with power control in wireless sensor networks

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The Journal of China Universities of Posts and Telecommunications 2007年第1期14卷 49-57页

作者： GUO Shu-jie ZHENG Jie QU yu-gui ZHAO Bao-hua pan Quan-ke Department of Electronic Engineering and Information Science University of Science and Technology of China Hefei 230027 China Department of Computer University of Science and Technology of China Hefei 230027 China

Clustering routing protocols excel in several aspects of wireless sensor networks （WSNs）. This article proposes a clustering and multihop routing protocol （CMRP）. In CMRP, a node independently makes its decision to compete for becoming a cluster head or join a cluster, according to its residual energy and average broadcast power of all its neighbors. To minimize the power consumption of the cluster head, CMRP sends the data in a power-aware multihop manner to the base station （BS） through a quasi-fixed route （QFR）. In addition, CMRP presents a transmission power control algorithm with dynamic intercluster neighbor position estimation （DCNPE） to save energy. Simulation results show that the performance of CMRP is better than the hybrid, energy-efficient, distributed clustering approach （HEED）. In the best case, CMRP increases the sensor network lifetime by 150.2%.

关键词： WSNs QFR DCNPE

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沈局重点工业污染源确定原则和评价方法的探讨

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铁道劳动安全卫生与环保 1996年第1期23卷 5-8页

作者：潘颖于克谷朝君王克宇郭淑华李笑敏沈阳铁路局环境监测站 110032 沈阳铁路局环境科学研究所沈阳铁路局环境保护办公室

根据沈局污染源管理的需要,对沈局污染源进行了重新评价,制定了确定重点工业污染源的3项原则:依量原则;依害原则;依位原则.遵循3项原则,我们选择了新的污染源评价方法,即对等标污染负荷法进行了加权处理.处理后的评价方法能够较为全面... 详细信息

根据沈局污染源管理的需要,对沈局污染源进行了重新评价,制定了确定重点工业污染源的3项原则:依量原则;依害原则;依位原则.遵循3项原则,我们选择了新的污染源评价方法,即对等标污染负荷法进行了加权处理.处理后的评价方法能够较为全面地反映污染源的潜在污染能力,是确定重点工业污染源的直接依据.

关键词：污染源优先控制等标污染负荷加权

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Possible multiple antimagnetic rotational bands in odd-A^(103,105)Pd and^(109)Cd nuclei

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Chinese Physics C 2022年第9期46卷 173-179页

作者： yu-Kun pan ke-Yan Ma Jing-Bin Lu College of Physics Jilin UniversityChangchun 130012China

The positive-parity signature partner bands in^(103,105)Pd and^(109)Cd nuclei are investigated using the classical particle-rotor *** on the systematic study of neighbouring nuclei,the signature partner bands of ^(105)Pd are assigned to theπ(g^(-4)_(9/2))■ν(g7/2h^(2)_(11/2))configuration,and this assignment is also supported by the present ***,the calculated$B(E2)$values of such bands of ^(105)Pd reproduce the experimental values well and exhibit a decrease with increasing angular momentum,suggesting that these two bands may originate from antimagnetic *** signature partner bands are also found in the neighboring^(103)Pd and^(109)Cd *** properties of both bands are in general agreement with the fingerprints of antimagnetic rotation,and thus the signature partner bands of^(103)Pd and^(109)Cd are suggested to be candidates for the multiple antimagnetic rotational bands of ^(105)*** addition,the evolution of the two-shears-like mechanism for possible multiple antimagnetic rotational bands in^(103,105)Pd and^(109)Cd nuclei is examined by investigating the orientation of the angular momenta.

关键词： signature partner band multiple antimagnetic rotation classical particle-rotor model

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Quantitative relationship between flagellate abundance and suspended particle density in Huanghai Sea and East China Sea in summer

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Acta Oceanologica Sinica 2006年第2期25卷 109-118页

作者： HUANG Lingfeng pan ke GUO Feng Department of Oceanography ＆ Institute of Subtropical Oceanography Xiamen University Xiamen 361005 China State key Laboratory of Marine Environmental Science Xiamen University Xiamen 361005 China

An investigation was carried out in the Huanghai Sea and the East China Sea to study the quantitative relationship between the abundance of flagellates and the density of suspended particles in the summer of 2001. The results show that the abundance of flagellates varies from 44-12 600 cell/cm^3, and flagellates sometimes constitutes a significant part of suspended particles. The size-spectra of suspended particles can be divided into four categories： flat spectrum, humped spectrum, plankton spectrum and mixed spectrum. In general, the abundance of flagellates varies in proportion to the density of suspended particles. However, their quantitative relations reveal different characteristics in the seawater samples of different types of particle-size spectrum. This is only a preliminary study of the quantitative relationship between flagellates and suspended particles, which might lead to a potential convenient approach to the estimation of flagellate abundance in the sea.

关键词： marine flagellates suspended particles particle-size spectra quantitative relationship

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