检索结果-南通市图书馆

Biochemical determination of lipid content in hepatic steatosis by the Soxtec method

World Journal of Gastroenterology 2010年第12期16卷 1495-1499页

作者： Elizabeth Hijona Lander Hijona Mikel Larzabal Cristina Sarasqueta pablo aldazabal Juan Arenas Luis Bujanda Department of Gastroenterology University of the Basque CountryDonostia HospitalCenter for Biomedical Research in Hepatic and Digestive Disease(CIBERehd) Department of Gastroenterology Basurto Hospital Department of Pathology Donostia Hospital Department of Epidemiology CIBERESPDonostia Hospital

AIM:To establish a quantitative method to measure the amount of ***:The livers of 53 male Wistar rats(225 g) with different degrees of hepatic steatosis were *** model of hepatic steatosis was based on a high carbohydrate,fat-free modified *** were classified into four grades depending on fat accumulation,using the Kleiner and Brunt *** fat was studied by the Soxtec method(SoxtecTM 2050 Auto Fat Extraction System),and agreement between both assays was assessed by calculating theκ ***:According to the histological classification,38% of rats presented grade 0,21% grade 1,22% grade 2 and 20% grade *** amount of fat per 100 g tissue was 2.60±0.64 g for grade 0,3.87±1.59 g for grade 1,5.82±1.37 g for grade 2 and 8.68± 2.30 g for grade *** significant differences were found between the mean values for each of the histological grades(P<0.05).The correlation for the quantification of fat in the liver between both assays was moderate(κ=0.60).CONCLUSION:The biochemical quantification of fat in liver tissue by the Soxtec method was correlated with the histological classification,although the agreement between the two tests was only moderate.

关键词： Liver steatosis Soxtec method Quantitative analysis Histology Biochemistry

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：