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Effects of isolation after sexual experience on anxiety-like, depressive-like behaviors and affective states in male rats

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Chinese Science Bulletin 2010年第36期55卷 4136-4142页

作者： WEN Fei1,2 & XU Lin1,3 1 Laboratory of Learning and Memory and Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming 650223, china 2 Graduate University of the Chinese Academy of Sciences, Beijing 100049, china 3 Mental Health Institute, the 2nd hospital of Central South University, Changsha 410011, china Laboratory of Learning and Memory and Key Laboratory of Animal Models and Human Disease Mechanisms Kunming Institute of Zoology the Chinese Academy of Sciences Kunming 650223 China Graduate University of the Chinese Academy of Sciences Beijing 100049 China Mental Health Institute the 2nd Hospital of Central South University Changsha 410011 China

Studies on social isolation have provided evidence for an enhanced anxiety-like phenotype, depressive-like symptoms and higher levels of 22-kHz ultrasonic vocalizations (USVs); whereas sexual experience could induce significant changes in affective states, decrease anxiety-and depressive-like responses. However, the behavioral and emotional alterations in male rats exposed to isolation after sexual experience remain poorly understood. Therefore, the present study examined anxiety-and depressive-like parameters, as well as USV emissions in male rats isolated for 1, 2, or 7 d following 1-week sexual (male-female paired housing) or social (male-male paired housing) interaction. Isolation for 1 d resulted in increased depressive-like symptoms, and 2-d isolation induced elevated anxiety-like behavior in sexually inexperienced rats, while rats with sexual experience exhibited comparable levels of emotionality at each isolation time. Moreover, isolated rats without sexual experience emitted less 22-and 50-kHz USVs, but sexually experienced rats exhibited similar affective states compared with control ones. These results suggested that prior sexual experience could have a modulatory effect on experimental rat emotional responses to isolation.

关键词：雄性大鼠情感状态焦虑抑郁行为情绪变化相互作用调节作用

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ApprOACH TO THE rEGIONALIZATION OF A GrICULTUrAL-pASTUrALIZATION ArOUND CHANGSHAN ISLANDS IN NOrTHErN YELLOW SEA

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Chinese Geographical Science 2002年第3期12卷 218-225页

作者： ZHANG Yao-guang,DONG Li-jing,LI Chun-ping(Institute of Marine resource,Liaon ing Normal University,Dalian 116029,p.r.china) Institute of Marine resource Liaoning Normal University Dalian P. R. China

Based on the data of resources,environment and foundation of productio n,applying principal compo-nents-clustering quantitative analysis,this article divides the maritime space of Changshan Islands into three regions of agricultural-pasturalization,pr oviding a scientific basis for the ra tional distribution of marine culti *** three re-gions are as follows:1)The region of an agricultural-pastu ralization in the northern part of ma ritime *** includes Da Wangjia and Shicheng *** ma in production is cultivation of praw n and molluscs in sea beach,float raft culture of mussel in shallow sea and scallop,and breeding of sea cucumber in subma rine.2)The region of agricultur-al-pasturalization in the middle we stern part of maritime *** includes Da Changshan and Guanglu island s,and west-ern part of Xiao Chengshan *** main production is float raft cultu re of mussel and scallop in shallow se a,and breed-ing of sea cucumber in submarine.3)The region of agricultural-pastura lization of the southeastern part of maritime *** includes the eastern part of Xiao ChangShan Island,Haiyang and Zhangzi *** main production is bre eding of abalone,sea cucumber,algaes and fish.

关键词： Changshan Islands agricultural-pasturalization of seash ore principal compo-nents-clustering analysis region alization

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Effect of angiotensin II receptor 1 antisense oligodoexynucleotides on physiological and pathophysiological growth of cardiomyocytes

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Acta pharmacologica Sinica 2004年第5期25卷 19-27页

作者： Ying WANG2, Jin-ming WANG2, Shu-xun Yan3, Ming-jiang LI2,4, Jian-jun LI22Department of Cardiology, renmin hospital, Wuhan University, Wuhan 430060, china 3Hubei College of Traditional Chinese Medicine, Wuhan 430061,china Department of Cardiology Renmin Hospital Wuhan University China Hubei College of Traditional Chinese Medicine China

AIM: To evaluate the role of angiotensin II receptor 1 antisense oligodexynucleotides (AT1r-AS-ODNs) on physi-ological and pathophysiological growth of cardiomyocytes from normotensive rats. METHODS: Cardiomyocyteswere transfected with AT1r-AS-ODNs (200 nmol/L) followed by treatment with or without angiotensin II (1 mol/L).In situ hybridization and western blot were used for AT1r mrNA and protein detection, respectively. c-Jun N-terminal protein kinase (JNK) activity was characterized by immune complex kinase assay. c-Jun protein expres-sion was examined by immunocytochemistry. DNA content was detected by flow cytometric assay. Atrial natri-uretic factor (ANF) expression was identified by radioimmunoassay. rESULTS: Treatment with AT1r-AS-ODNsfor 24 h resulted in 51.2 % decrease in AT1r mrNA and 60.7 % in protein (prol). However, the basallevel of JNK activity, c-Jun protein expression, and DNA content were not altered by AT1r-AS treatment in absenceof overactive hormonal system. After treatment with angiotensin II for 30 min, both p46JNK and p54JNK wererobustly activated. By 2 h, c-Jun protein expression was increased. By 24 h, angiotensin II caused a markedincrease both in G0/G1 and G2/M DNA content, and increased ANF expression by 1.8-fold. All these were inhibitedby AT1r-AS-ODNs pretreatment. In contrast, sense sequence was ineffective. CONCLUSION: Decrease ofAT1r expression by AS-ODNs did not interfere with normal growth, but protected cardiomyocytes from angio-tensin II-dependent pathophysiological growth.

关键词： antisense oligodeoxynucleotides angiotensin receptors angiotensin II myocardium hypertrophy mitogen-activated protein kinases

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The Study of Treating Mental retarded Children with Scalp Acupuncture

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中国针灸 1995年第S2期15卷 314-315页

作者： Liu Zhen huan(No.2 people’s hospital,Yangquan,Shanxi,china)

ＴｈｅＳｔｕｄｙｏｆＴｒｅａｔｉｎｇＭｅｎｔａｌＲｅｔａｒｄｅｄＣｈｉｌｄｒｅｎｗｉｔｈＳｃａｌｐＡｃｕｐｕｎｃｔｕｒｅ￥ＬｉｕＺｈｅｎｈｕａｎ（Ｎｏ．２Ｐｅｏｐｌｅ＇ｓＨｏｓｐｉｔａｌ，Ｙａｎｇｑｕａｎ，Ｓｈａｎｘ... 详细信息

ＴｈｅＳｔｕｄｙｏｆＴｒｅａｔｉｎｇＭｅｎｔａｌＲｅｔａｒｄｅｄＣｈｉｌｄｒｅｎｗｉｔｈＳｃａｌｐＡｃｕｐｕｎｃｔｕｒｅ￥ＬｉｕＺｈｅｎｈｕａｎ（Ｎｏ．２Ｐｅｏｐｌｅ＇ｓＨｏｓｐｉｔａｌ，Ｙａｎｇｑｕａｎ，Ｓｈａｎｘｉ，Ｃｈｉｎａ）Ｍｅｎｔａｌｒｅｔ...

关键词： Acupuncture

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One　Needle　More　Functions　about　Neiguan　Acupoint　penetrating　Waiguan　Acupoint

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中国针灸 1995年第S2期15卷 336-337页

作者： Wen Qinghua(The No.2 people’s hospital Nehe CityHeilongjiang province,china)

ＯｎｅＮｅｅｄｌｅＭｏｒｅＦｕｎｃｔｉｏｎｓａｂｏｕｔＮｅｉｇｕａｎＡｃｕｐｏｉｎｔＰｅｎｅｔｒａｔｉｎｇＷａｉｇｕａｎＡｃｕｐｏｉｎｔ￥ＷｅｎＱｉｎｇｈｕａ（ＴｈｅＮｏ．２Ｐｅｏｐｌｅ＇ｓＨｏｓｐｉｔａｌＮｅｈｅＣ... 详细信息

ＯｎｅＮｅｅｄｌｅＭｏｒｅＦｕｎｃｔｉｏｎｓａｂｏｕｔＮｅｉｇｕａｎＡｃｕｐｏｉｎｔＰｅｎｅｔｒａｔｉｎｇＷａｉｇｕａｎＡｃｕｐｏｉｎｔ￥ＷｅｎＱｉｎｇｈｕａ（ＴｈｅＮｏ．２Ｐｅｏｐｌｅ＇ｓＨｏｓｐｉｔａｌＮｅｈｅＣｉｔｙＨｅｉｌｏｎｇｊｉａｎｇＰｒ...

关键词： Function

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Anatomy and physiology of peripheral auditory system and commen causes of hearing loss

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Journal of Otology 2009年第1期4卷 -页

作者： ZHANG Qing1,DUAN Mao-li1-4 1 Department of Otorhinolarygology,The 2nd Affiliated hospital of Xi’an Jiaotong University,School of Medicine,Xi’an Jiaotong University,Xi’an,p.r.china 2 Department of clinical neuroscience,3,Department of Otolaryngology,Karolinska hospital,171 76 Stockholm,Sweden 4 Department of Otolaryngology,Affiliated hospital of Anhui Medical University,china Department of Otorhinolarygology The 2nd Affiliated Hospital of Xi’ an Jiaotong UniversitySchool of Medicine Xi’ an Jiaotong University Xi’ an P.R.China Department of clinical neuroscience Karolinska Hospital 171 76 Stockholm Sweden Department of Otolaryngology Karolinska Hospital 171 76 Stockholm Sweden Department of Otolaryngology Affiliated Hospital of Anhui Medical University China

For Otolaryngologist,it is the most important to know the principle of anatomy,physiology and common *** but more concise summary has been sum up in the following review in order to help young ENT doctor to understand... 详细信息

关键词： inner hair cell outer hair cell spiral ganglion neuron ototoxicity

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Establisment of a novel pig model of pancreatoduodenal allotransplantation

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Hepatobiliary & pancreatic Diseases International 2005年第2期4卷 192-196页

作者： Jian-Shui Li, Yong Tang, Jiang-Tao Wei and Zhao-Da Zhang Chengdu, china Department of General Surgery, west china hospital, Sichuan University, Chengdu 610041 , china and Department of Anesthesia, Chengdu railway Central hospital, Chengdu 610081, china Department of General Surgery West China HospitalSichuan UniversityChengdu 610041 China.

BACKGrOUND: pancreas transplantation is a valid thera- py for patients with type 1 diabetes mellitus (IDDM). This study was undertaken to establish a stable pig model of whole pancreatoduodenal allotransplantation of physiologi- cally coincidence for pancreas transplantation. METHODS: The IDDM pig model was made by resection of the pancreas from the recipient. The portal vein of the donor was end-to-side anastomosed to the anterior mesen- teric vein (equal to the superior mesenteric vein) or the portal vein of the recipient. The far end of the celiac axis was anastomosed to each other. With end-to-side fashion, the duodenum of the donor was anastomosed to the jeju- num of the recipient. rESULTS: In 16 transplantations, results were satisfactory. The anatomical results and immune function including the function of the pancreas were similar to those of human be- ing. CONCLUSION: Of the pancreas of the pig, this study of pancreatic transplantation shows that the novel pig whole pancreatoduodenal allotransplantation model is ideal.

关键词： pancreatoduodenal transplantation model diabetes pig

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中国膝骨关节炎康复治疗指南(2023版)

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中国循证医学杂志 2024年第1期24卷 1-14页

作者：中华医学会物理医学与康复学分会四川大学华西医院杨霖何成奇朱思忆张玉婷杨梦璇不详四川大学华西医院康复医学中心

膝骨关节炎(KOA)是最常见的关节退行性疾病,是我国最常见致残疾病之一,给患者、家庭和社会造成巨大的经济负担。康复治疗是KOA疾病管理的核心与基础,规范化KOA康复治疗是疾病防治、促进医疗服务高质量发展的重要手段。本指南在2016、201... 详细信息

膝骨关节炎(KOA)是最常见的关节退行性疾病,是我国最常见致残疾病之一,给患者、家庭和社会造成巨大的经济负担。康复治疗是KOA疾病管理的核心与基础,规范化KOA康复治疗是疾病防治、促进医疗服务高质量发展的重要手段。本指南在2016、2019年版基础上进行更新,由中华医学会物理医学与康复学分会和四川大学华西医院牵头发起,指南制订以国内外指南制订方法与原则为指导,通过临床问题筛选与解构、多轮德尔菲问卷咨询等方法,遴选出两大类(康复评定与治疗)11个核心临床问题。以《国际功能、残疾和健康分类》(ICF)为理论架构,基于当前可获得的最佳证据,采用推荐意见分级的评估、制订及评价(GrADE)方法对证据质量及推荐意见进行分级,同时采用GrADE从证据到决策的框架(EtD)辅助由证据向推荐意见转化,按照国际临床实践指南报告标准(rIGHT)进行报告,充分考虑患者意愿及价值观,并结合中国临床实践需求,最终形成11个要点和28条推荐意见,以期系统性规范膝骨关节炎康复治疗。

关键词：膝骨关节炎康复评定康复治疗临床实践指南循证医学

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Association between ICU quality and in-hospital mortality of V-V ECMO-supported patients—the ECMO quality improvement action(EQIA)study:a national cohort study in china from 2017 to 2019

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Frontiers of Medicine 2024年第2期18卷 315-326页

作者： Wei Cheng Jieqing Chen Xudong Ma Jialu Sun Sifa Gao Ye Wang Longxiang Su Lu Wang Wei Du Huaiwu He Yujie Chen Zunzhu Li Qi Li Jianhua Sun Hongbo Luo Jinbang Liu Guangliang Shan Bing Du Yanhong Guo Dawei Liu Chang Yin Xiang Zhou on behalf of the china National Critical Care Quality Control Center-the ECMO quality improvement action(EQIA)study Department of Critical Care Medicine State Key Laboratory of Complex Severe and Rare DiseasesPeking Union Medical College HospitalChinese Academy of Medical Science and Peking Union Medical CollegeBeijing100730China Information Center Department/Department of Information Management State Key Laboratory of Complex Severe and Rare DiseasesPeking Union Medical College HospitalPeking Union Medical College&Chinese Academy of Medical SciencesBeijing100730China Department of Medical Administration National Health Commission of the People’s Republic of ChinaBeijing100044China School of population Medicine and public Health Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing100730China Department of Epidemiology and Biostatistics Institute of Basic Medicine SciencesChinese Academy of Medical Sciences(CAMS)&School of Basic MedicinePeking Union Medical CollegeBeijing100730China National Institute of hospital Administration National Health Commission of the People’s Republic of ChinaBeijing100044China 不详

This cohort study was performed to explore the influence of intensive care unit(ICU)quality on in-hospital mortality of veno-venous(V-V)extracorporeal membrane oxygenation(ECMO)-supported patients in *** study involved all V-V ECMO-supported patients in 318 of 1700 tertiary hospitals from 2017 to 2019,using data from the National Clinical Improvement System and china National Critical Care Quality Control *** quality was assessed by quality control indicators and capacity *** the 2563 V-V ECMO-supported patients in 318 hospitals,a significant correlation was found between ECMO-related complications and *** reintubation rate within 48 hours after extubation and the total ICU mortality rate were independent risk factors for higher in-hospital mortality of V-V ECMO-supported patients(cutoff:1.5%and 7.0%;95%confidence interval:1.05–1.48 and 1.04–1.45;odds ratios:1.25 and 1.23;p=0.012 and p=0.015,respectively).Meanwhile,the V-V ECMO center volume was a protective factor(cutoff of≥50 cases within the 3-year study period;95%confidence interval:0.57–0.83,odds ratio:0.69,p=0.0001).The subgroup analysis of 864 patients in 11 high-volume centers further strengthened these ***,ICU quality may play an important role in improving the prognosis of V-V ECMO-supported patients.

关键词： veno-venous extracorporeal membrane oxygenation in-hospital mortality high-volume centers quality control intensive care unit capacity parameters

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Temporal and Tight Hepatitis C Virus Gene Activation in Cultured Human Hepatoma Cells Mediated by a Cell-permeable Cre recombinase

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Acta Biochimica et Biophysica Sinica 2004年第10期36卷 687-694页

作者： Dong XIAO*, Kang XU1, Ying YUE, Zhong-Min GUO, Bing HUANG, Xin-Yan DENG, Huan TANG2, and Xi-Gu CHEN* Center of Experimental Animals, Zhongshan University, Guangzhou 510080, china 1The first affiliated hospital of Sun Yat-sen University, Zhongshan University, Guangzhou 510080, china 2The Third Military Medical University, Chongqing 400010, china Center of Experimental Animals Zhongshan University The first affiliated hospital of Sun Yat-sen University Zhongshan University The Third Military Medical University

Conditional gene expression has greatly facilitated the examination of the functions of particular gene products. Using the Cre/lox p switching expression system, we plan to develop efficient conditional transgeneactivation of hepatitis C virus core protein (HCV-C) cDNA (nucleotide 342–914) in the transgenic mice to overcome “immune tolerance” formed during the embryonic period and “immune escape” against hepatitis virus antigen in our project. To use this system in vivo, the dormant transgenic construct, i.e., pApoE-SCS-EGFp-HCV-C, was generated using techniques of standard molecular biology. The liver- specific human apoE promoter was here used to target expression of genes of interest (EGFp and HCV-C) to murine liver. prior to generating the transgenic mice, the availability of Cre/lox p system and construct functionality were successfully verified by a cell-free recombination system and via checking the expression of EGFp and HCV-C in the human hepatoma cells at the mrNA and protein levels. These results suggest that the Cre/lox p system could tightly control expression of EGFp and HCV-C in vitro, which laid a solid foundation to conditionally activate expression of target gene(s) in transgenic mice by Cre-mediated site-specific recombination.

关键词： hepatitis C virus Cre/lox p switching expression system cell-free and intracellular recombination systems cell-permeable Cre recombinase EGFp human hepatoma cells

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