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Introgression of sharp eyespot resistance from Dasypyrum villosum chromosome 2VL into bread wheat

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作物学报:英文版 2023年第5期11卷 1512-1520页

作者： Caiyun Liu Wei Guo Yang Wang Bisheng Fu Jaroslav Doležel Ying Liu Wenling Zhai Mahmoud Said István Molnár Kateřina Holušová Ruiqi Zhang Jizhong Wu Institute of Germplasm Resources and Biotechnology/Jiangsu provincial Key Laboratory of Agrobiology Jiangsu Academy of Agricultural SciencesNanjing 210014JiangsuChina College of Agronomy/JCIC-MCp/National Key Lab of Crop Genetics and Germplasm Enhancement Nanjing Agricultural UniversityNanjing 210095JiangsuChina Institute of Experimental Botany Centre of the HanáRegion for Biotechnological and Agricultural ResearchŠlechtitelů31CZ-783671 Olomoucthe Czech Republic Field Crops Research Institute Agricultural Research Centre9 Gamma StreetGiz112619 CairoEgypt Agricultural Institute Centre for Agricultural ResearchELKHMartonvásár 2462Hungary Jiangsu Co-Innovation Center for Modern production Technology of Grain Crops Yangzhou UniversityYangzhou 225009JiangsuChina Zhongshan Biological Breeding Laboratory Nanjing 210014JiangsuChina

Wheat sharp eyespot, a stem disease caused by the soilborne fungus Rhizoctonia cerealis van der Hoeven,has become a threat to wheat production worldwide. Exploiting resistance resources from wild relatives of wheat is a promising strategy for controlling this disease. In this study, a new wheat–Dasypyrum villosum T2DS·2V#4L translocation line in the background of Chinese Spring(CS) showed stable resistance to R. cerealis. Introgression of the T2DS·2V#4L chromosome into wheat cultivar Aikang 58 by backcrossing produced a marked increase in sharp eyespot resistance in NIL-T2DS·2V#4L in comparison with NILT2DS·2DL, and no detrimental effects of 2V#4L on agronomic traits were observed in the BC2F2, BC2F2:3,and BC2F2:4generations. Flow-sorted sequencing of 2V#4L yielded 384.3 Mb of assembled sequence, and8836 genes were predicted of which 6154 had orthologs in at least one of the 2AL, 2BL, and 2DL arms of CS, whereas 1549 genes were unique to 2V#4L. About 100,000 SNps were detected in genes of 2V#4L and2DL in 10 sequenced bread wheat cultivars. A Kompetitive Allele Specific polymerase chain reaction and30 conserved ortholog sequence markers were developed to trace the 2V#4L chromatin in wheat backgrounds. T2DS·2V#4L compensating translocation lines represent novel germplasm with sharp eyespot resistance and the markers will allow rapid detection in breeding programs.

关键词： Triticum aestivum Rhizoctonia cerealis Dasypyrum villosum Flow sorting KASp

A COMpREHENSIVE STUDY OF FRETTING FATIGUE OF STRUCTURAL ALLOYS IN SYMMETRIC TENSION-COMpRESSION

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A COMPREHENSIVE STUDY OF FRETTING FATIGUE OF STRUCTURAL ALLO...

Increase of Alternative and Renewable Energy Sources Utilization in Slovakia by 2020 in Comparison to Other Selected EU Countries

第三届摩擦疲劳学国际会议

作者： G.V. TSYBAN’ov Yu. KURASH Institute for problems of Strength National Academy of Sciences of Ukraine 2 Timiryazevskaya str. Kiev-14 01014 Ukraine Institute for problems of Strength National Academy of Sciences of Ukraine 2 Timiryazevskaya str. Kiev-14 01014 Ukraine

＜正＞A method of fatigue testing is proposed for fretting pairs of structural alloys. The distinctive feature of the method is a possibility of measuring and controlling contact pressure, friction forces, and sliding amplitudes using electric signals with a subsequent preliminary processing, filtering and accumulation of the tests results in a computer. The authors justify the use of the value of electrie microcurrents occurring in the fretting pair in the course of testing as a parameter for the evaluation of the level of damaging processes of nonmechanical nature.

关键词： Fretting fatigue Damaging processes Tribocurrents Data processing

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Increase of Alternative and Renewable Energy Sources Utiliza...

2012 International Conference on Mechanical Engineering and Materials(ICMEM 2012)

作者： pAVOLová Henrieta SE?ová Andrea BAKALáR Tomá? Technical University of Ko?ice Faculty of MiningEcologyProcess Control and GeotechnologiesInstitute of Business and Management

One way to save fuel is to use renewable energy,including solar,wind,hydro,geothermal and *** use of renewable and alternative energy sources is contributing to environmental protection and nature conservation as a whole and provides a perspective of sustainable *** aim of this paper is to compare the possibility of increasing the use of alternative and renewable energy sources in Slovakia with neighboring *** determines and also identifies the level of the Slovak Republic in the use of renewable energy sources because it is expected to increase by 2020 in each *** paper is also devoted to the theoretical base of renewable and alternative forms of *** part of the paper deals with an analysis and comparison of the state of the use of renewable energy sources in Slovakia and in selected EU *** part deals with the overall national objectives set by the European Union for the proportion of energy from RES,which will be achieved by *** paper is a partial result of the research project on RES realized at Technical University of Ko?ice（Slovakia）.

关键词： renewable energy sources impacts on the environment EU countries statistical analysis SWOT analysis

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时滞系统控制算法的改进

基础自动化 2000年第6期7卷 9-10,39页

作者：韩绍坤 Nоviкov V.А . 北京理工大学北京100081 St.petersburg ETU russia197396

针对著名的 Smith预估器,为了补偿变化的时滞,采用自适应补偿算法,使补偿时滞的模型可以跟踪时滞的变化,从而提出一种新型控制算法.仿真结果表明,改进的方案可以明显地提高控制系统精度.

关键词：时滞系统控制算法 Smith预估器自适应控制

Analysis of Higher Order System with Impulse Exciting Functions in Z-Domain

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Circuits and Systems 2016年第11期7卷 3951-3970页

作者： Branislav Dobrucký pavol Štefanec Mariana Beňová Oleg V. Chernoyarov Michal pokorný Faculty of Electrical Engineering University of ?ilina ?ilina Slovak Republic Department of Radio Engineering Devices and Antenna Systems National Research University Moscow Power Engineering Institute Moscow Russia

This paper deals with mathematical modelling of impulse waveforms and impulse switching functions used in electrical engineering. Impulse switching functions are later investigated using direct and inverse z-transformation. The results make possible to present those functions as infinite series expressed in pure numerical, exponential or trigonometric forms. The main advantage of used approach is the possibility to calculate investigated variables directly in any instant of time;dynamic state can be solved with the step of sequences (T/6, T/12) that means very fast. Theoretically derived waveforms are compared with simulation worked-out results as well as results of circuit emulator LT spice which are given in the paper.

关键词： Impulse Systems Switching Function Z-Transformation Inverse Z-Transformation Steady State Operation Dynamical State Model Modelling and Simulation

娱乐新贵 Hp pavilion m8090cn

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数码先锋 2007年第5期 30-31页

作者： ov

pavilion m8090cn是Hp今年春季的旗舰产品,它采用近期流行的黑色设计,配备22英寸“黑晶”宽屏液晶显示器,在神秘中展现王者风范。作为家庭数字娱乐管理中枢,Hp pavilion m8090cn凭借其出众的时尚外观和无敌的娱乐性能进占客厅,荣登新宠... 详细信息

pavilion m8090cn是Hp今年春季的旗舰产品,它采用近期流行的黑色设计,配备22英寸“黑晶”宽屏液晶显示器,在神秘中展现王者风范。作为家庭数字娱乐管理中枢,Hp pavilion m8090cn凭借其出众的时尚外观和无敌的娱乐性能进占客厅,荣登新宠王座。

关键词： pavilion 宽屏液晶数字娱乐旗舰产品王者风范双核处理器酷睿

Homogenization of twin-roll cast AA8079 aluminum alloy studied by in-situ TEM

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Transactions of Nonferrous Metals Society of China 2022年第7期32卷 2138-2149页

作者： R.KRÁLÍK B.KŘIVSKÁ L.BAJTOŠovÁ M.ŠLApÁKovÁ M.CIESLAR Faculty of Mathematics and physics Charles UniversityKe Karlovu 312116 Prague 2Czechia

AA8079 is a commonly used stock material for manufacturing thin packaging *** primary alloying elements Fe and Si can form binary and tertiary ***-situ TEM simulating homogenization annealing process of the as-cast material was used to analyze the real-time changes of the shape,type,and distribution of these *** affect the mechanical properties of the final product and susceptibility of the material to the formation of pinholes and other macroscopic *** set of as-cast samples were annealed in a regime simulating industrial treatment in combination with measurements of resistivity to validate the results of the in-situ *** results show clear temperature intervals of recovery,matrix desaturation,and phase transformations occurring in several stages:spheroidization of the original particles above 450℃,nucleation of new particles at 475℃,particles coarsening above 525℃,and an entire dissolution of the original particles above 550℃.

关键词： aluminum alloys in-situ annealing homogenization foil stock materials

Significance of postoperative follow-up of patients with metastatic colorectal cancer using circulating tumor DNA

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World Journal of Gastroenterology 2019年第48期25卷 6939-6948页

作者： Lucie Benešová Tereza Hálková Renata ptáčková Anastasiya Semyakina Kateřina Menclová Jiřípudil Miroslav Ryska Miroslav Levý JaromírŠimša Filip pazdírek JiříHoch Milan Blaha Marek Minárik Centre for Applied Genomics of Solid Tumors Genomac Research InstitutePrague CZ 16100Czech Republic Surgery Department 2nd Faculty of Medicine of Charles University Prague and Military University HospitalPrague CZ 16902Czech Republic Surgery Department 1st Faculty of MedicineCharles University Prague and Thomayer HospitalPrague CZ 14059Czech Republic Surgery Department 2nd Faculty of Medicine of Charles University Prague and Motol University HospitalPrague CZ 15006Czech Republic Institute of Biostatistics and Analyses of the Masaryk University Brno CZ 62500Czech Republic Elphogene Prague CZ 16100Czech Republic

BACKGROUND One of the most notable applications for circulating tumor DNA(ctDNA)detection in peripheral blood of patients with metastatic colorectal cancer(mCRC)is a long-term postoperative *** referred to as a“liquid(re)biopsy”it is a minimally invasive procedure and can be performed repeatedly at relatively short intervals(months or even weeks).The presence of the disease and the actual extent of the tumor burden(tumor mass)within the patient’s body can be *** is of particular importance,especially when evaluating radicality of surgical treatment as well as for early detection of disease progression or *** To confirm the radicality of surgery using ctDNA and compare available methods for detection of recurrence in metastatic colorectal *** total of 47 patients with detected ctDNA and indications for resection of mCRC were enrolled in the multicenter study involving three surgical *** postoperative follow-ups using imaging techniques and the determination of tumor markers were supplemented by ctDNA *** addition to the baseline ctDNA testing prior to surgery,a postoperative observation was conducted by evaluating ctDNA presence up to a week after surgery and subsequently at approximately three-month *** presence of ctDNA was correlated with radicality of surgical treatment and the actual clinical status of the *** Among the monitored patients,the R0(curative)resection correlated with postoperative ctDNA negativity in 26 out of 28 cases of surgical procedures(26/28,93%).In the remaining cases of R0 surgeries that displayed ctDNA,both patients were diagnosed with a recurrence of the disease after 6 *** 7 patients who underwent an R1 resection,4 ctDNA positivities(4/7,57%)were detected after surgery and associated with the confirmation of early disease recurrence(after 3 to 7 months).All 15 patients(15/15,100%)undergoing R2 resection remained constantly ctDNA positive dur

关键词： Circulating tumor DNA Metastatic colorectal cancer postoperative Radicality of resection Follow-up Recurrence

Virome identification in wheat in the Czech Republic using small RNA deep sequencing

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Journal of Integrative Agriculture 2020年第7期19卷 1825-1833页

作者： Khushwant SINGH Jana JAROSovA Jan FOUSEK CHEN Huan Jiban Kumar KUNDU plant Virus and Vector Interactions Group Division of Crop Protection and Plant HeatthCrop Research InstitutePrague 16106Czech Republic Zhejiang Tianke High-Technology Development Co. Ltd.Hangzhou 310012P.R.China

High-throughput deep-sequencing technology and bioinformatics analysis of the small RNA(sRNA)population isolated from plants allows universal virus detection and complete virome reconstruction for a given *** the present sRNA deep-sequencing analysis of virus-infected wheat samples in the Czech Republic,samples were firstly tested for barley yellow dwarf viruses(BYDVs),wheat streak mosaic virus(WSMV)and wheat dwarf virus(WDV)using ELISA,RT-pCR and *** sRNA sequencing of these samples yielded more than^60 million single-end 50-bp reads with high confidence for nine field samples of ***,16.5%of reads were virus-specific and 83.5%were mapped to the *** 21-nt reads(-7.7E+06 reads)were found than 24-nt(~6.20E+06 reads)or 22-nt(-4.30E+06 reads)*** novo assembly of the high-quality contigs revealed the presence of three earlier repoted viruses in the Czech Republic:BYDVs(31.48%),WSMV(24.23%)and WDV(2666%).We also showed the presence of cereal yellow dwarf virus(14.33%;two species CYDV-RpS and CYDV-RpV family Luteoviridae/polerovirus)and wheat yellow dwarf virus(WYDV,3.30%;Luteoviridae).phylogenetic analysis showed CYDV and WYDV grouped separately from ***,several recombination breakpoints were found among the groups of yellow dwarf viruses(BYDVs,CYDV,and WYDV).Using RNA deep sequencing,we confirmed the presence of the three known viruses(BYDVs,WSMV,and WDV)and the first record of two species of CYDV and WYDV in wheat in the Czech Republic.

关键词： NGS sRNA BYDVs CYDV WSMV WYDV WDV