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A facile strategy for tuning the density of surface-grafted biomolecules for melt extrusion-based additive manufacturing applications

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bio-Design and Manufacturing 2024年第3期7卷 277-291页

作者： I.A.O.beeren G.Dos Santos p.J.Dijkstra C.Mota J.bauer H.Ferreira Rui L.reis N.Neves S.Camarero-Espinosa M.b.baker L.Moroni Department of Complex Tissue Regeneration MERLN Institute for Technology-Inspired Regenerative MedicineMaastricht University6229 ER MaastrichtThe Netherlands 3b’s Research Group I3Bs–Research Institute on BiomaterialsBiodegradables and BiomimeticsHeadquarters of the European Institute of Excellence on Tissue Engineering and Regenerative MedicineUniversity of MinhoAveParkZona Industrial da Gandra4805-017 BarcoGuimarãesPortugal ICVS/3b’s-pT Government Associate Laboratory 4806-909 Braga/GuimarãesPortugal pOLYMAT University of the Basque Country UPV/EHU20018 Donostia/San SebastiánSpain IKERbASQUE Basque Foundation for Science48009 BilbaoSpain

Melt extrusion-based additive manufacturing(ME-AM)is a promising technique to fabricate porous scaffolds for tissue engi-neering ***,most synthetic semicrystalline polymers do not possess the intrinsic biological activity required to control cell *** of biomolecules on polymeric surfaces of AM scaffolds enhances the bioactivity of a construct;however,there are limited strategies available to control the surface ***,we report a strategy to tune the surface density of bioactive groups by blending a low molecular weight poly(ε-caprolactone)5k(pCL5k)containing orthogonally reactive azide groups with an unfunctionalized high molecular weight pCL75k at different *** porous three-dimensional(3D)scaf-folds were then fabricated using a high weight percentage(75 wt.%)of the low molecular weight pCL *** a proof-of-concept test,we prepared films of three different mass ratios of low and high molecular weight polymers with a thermopress and reacted with an alkynated fluorescent model compound on the surface,yielding a density of 201-561 pmol/cm^(2).Subsequently,a.b.ne morphogenetic protein 2(bMp-2)-derived peptide was grafted onto the films comprising different blend compositions,and the effect of peptide surface density on the osteogenic differentiation of human mesenchymal stromal cells(hMSCs)was *** two weeks of culturing in a.b.sic medium,cells expressed higher levels of bMp receptor II(bMpRII)on films with the conjugated *** addition,we found that alkaline phosphatase activity was only significantly enhanced on films contain-ing the highest peptide density(i.e.,561 pmol/cm^(2)),indicating the importance of the surface *** together,these results emphasize that the density of surface peptides on cell differentiation must be considered at the cell-material ***,we have presented a viable strategy for ME-AM community that desires to tune the bulk and surface functionality via.b.ending of(m

关键词： Additive manufacturing blending Surface functionalization Surface density Click chemistry Human

Clinical implications of single cell sequencing for bladder cancer

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Oncology Research 2024年第4期32卷 597-605页

作者： REZA YADOLLAHVANDMIANDOAb MEHRSA JALALIZADEH FRANCIELE ApARECIDA VECHIA DIONATO KEINI bUOSI pATRÍCIA A.F.LEME LUCIANA S.b.DAL COL CRISTIANE F.GIACOMELLI ALEX DIAS ASSIS NASIM bASHIRICHELKASARI LEONARDO OLIVEIRA reis UroScience School of Medical SciencesUniversity of CampinasUNICAMPCampinasSao Paulo13083-872Brazil ImmunOncology Pontifical Catholic University of CampinasPUC-CampinasCampinasSao Paulo13087-571Brazil

bladder cancer(bC)is the 10th most common cancer worldwide,with about 0.5 million reported new cases and about 0.2 million deaths per *** this scoping review,we summarize the current evidence regarding the clinical implications of single-cell sequencing for bladder cancer based on pRISMA *** searched pubMed,CENTRAL,Embase,and supplemented with manual searches through the Scopus,and Web of Science for published studies until February *** included original studies that used at least one single-cell technology to study bladder ***-one publications were included in the ***-nine studies showed that this technology can identify cell subtypes in the tumor microenvironment that may predict prognosis or response to immune checkpoint inhibition *** studies were able to diagnose bC by identifying neoplastic cells through single-cell sequencing urine *** remaining studies were mainly a preclinical exploration of tumor microenvironment at single cell ***-cell sequencing technology can discriminate heterogeneity in bladder tumor cells and determine the key molecular properties that can lead to the discovery of novel perspectives on cancer *** nascent tool can advance the early diagnosis,prognosis judgment,and targeted therapy of bladder cancer.

关键词： bladder cancer Urothelial carcinoma Transitional cell carcinoma Single-cell sequencing Tumor heterogeneity Immunotherapy Scoping review Tumor microenvironment

Highly sensitive ratiometric fluorescent fiber matrices for oxygen sensing with micrometer spatial resolution

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bio-Design and Manufacturing 2024年第3期7卷 292-306页

作者： Giuliana Grasso Valentina Onesto Stefania Forciniti Eliana D’Amone Francesco Colella Lara pierantoni Valeria Famà Giuseppe Gigli Rui L.reis J.Miguel Oliveira Loretta L.del Mercato nstitute of Nanotechnology National Research Council(CNR-NANOTEC)c/o Campus Ecoteknevia Monteroni73100 LecceItaly Department of Mathematics and physics“Ennio De Giorgi” University of Salentoc/o Campus Ecoteknevia Monteroni73100 LecceItaly 3b’s Research Group I3Bs–Research Institute on BiomaterialsBiodegradables and BiomimeticsHeadquarters of the European Institute of Excellence on Tissue Engineering and Regenerative MedicineUniversity of MinhoAveParkZona Industrial da Gandra4805-017 BarcoGuimarãesPortugal ICVS/3b’s-pT Government Associate Laboratory 4806-909 Braga/GuimarãesPortugal Department of Experimental Medicine University of Salentoc/o Campus Ecoteknevia Monteroni73100 LecceItaly

Oxygen(O_(2))-sensing matrices are promising tools for the live monitoring of extracellular O_(2) consumption levels in long-term cell *** this study,ratiometric O_(2)-sensing membranes were prepared by electrospinning,an easy,low-cost,scalable,and robust method for fabricating ***(ε-caprolactone)and poly(dimethyl)siloxane polymers were blended with tris(4,7-diphenyl-1,10-phenanthroline)ruthenium(II)dichloride,which was used as the O_(2)-sensing probe,and rhodamine b isothiocyanate,which was used as the reference *** functionalized scaffolds were morphologically characterized by scanning electron microscopy,and their physicochemical profiles were obtained by Fourier transform infrared spectroscopy,thermogravimetric analysis,and water contact angle *** sensing capabilities were investigated by confocal laser scanning microscopy,performing photobleaching,reversibility,and calibration curve studies toward different dissolved O_(2)(DO)*** sensing nanofibers showed a high response to changes in DO concentrations in the physiological-pathological range from 0.5%to 20%and good stability under ratiometric *** addition,the sensing systems were highly biocompatible for cell growth promoting adhesiveness and growth of three cancer cell lines,namely metastatic melanoma cell line SK-MEL2,breast cancer cell line MCF-7,and pancreatic ductal adenocarcinoma cell line panc-1,thus recreating a suitable biological environment in *** O_(2)-sensing biomaterials can potentially measure alterations in cell metabolism caused by changes in a.b.ent O_(2)content during drug testing/validation and tissue regeneration processes.

关键词： Electrospinning Ruthenium(II)dichloride Oxygen sensors Ratiometric imaging Fluorescence

plant growth and metabolism of exotic and native Crotalaria species for mine land rehabilitation in the Amazon

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Journal of Forestry Research 2024年第1期35卷 143-154页

作者： Hemelyn Soares das Chagas Rafael Silva Guedes Markus Gastauer paula Godinho Ribeiro Allan Klynger da Silva Lobato Cecílio Frois Caldeira Fabrício William deÁvila AndréRodrigues dos reis Silvio Junio Ramos Instituto Tecnológico Vale Rua Boaventura da Silva955BelémPA 66055-090Brazil Universidade Federal do Sul e Sudeste do pará XinguaraPABrazil Núcleo de pesquisa Vegetal básica e Aplicada Universidade Federal Rural da AmazôniaParagominasPABrazil Departamento de Engenharia Florestal Universidade Estadual do Centro-Oeste/UNICENTROIratiPRBrazil School of Sciences and Engineering São Paulo State University(UNESP)TupãSP.CEP 17602-496Brazil

Despite its enormous benefits,mining is respon-sible for intense changes to vegetation and soil ***,after extraction,it is necessary to rehabilitate the mined areas,creating better conditions for the establishment of plant species which is *** study evaluated mineral and organic fertilization on the growth,and ca.b.n and nitrogen(N)metabolism of two Crotalaria species[Cro-talaria spectabilis(exotic species)and Crotalaria maypu-rensis(native species from Carajás Mineral province(CMp)]established on a waste pile from an iron mine in CMp.A control(without fertilizer application)and six fertilization mixtures were tested(i=NpK;ii=NpK+micronutrients;iii=NpK+micronutrients+organic compost;iv=pK;v=pK+micronutrients;vi=pK+micronutrients+organic compost).Fertilization contributed to increased growth of both species,and treatments with NpK and micronutrients had the best results(up to 257%***),while organic fertilization did not show *** Crotalaria had a greater number of nodules,higher nodule dry mass,chlorophyll a and b contents and showed free ammonium as the predominant N form,reflecting greater increments in biomass compared to native *** having lower growth,the use of this native species in the rehabilitation of mining areas should be considered,mainly because it has good development and meets current government legislation as an opportunity to restore local biodiversity.

关键词： biological nitrogen fixation Mining Nitrogen metabolism plant nutrition

Liver transplantation as an alternative for the treatment of perihilar cholangiocarcinoma: A critical review

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Hepatobiliary & pancreatic Diseases International 2024年第2期23卷 139-145页

作者： Wellington Andraus Francisco Tustumi Alexandre Chagas Santana Rafael Soares Nunes pinheiro Daniel reis Waisberg Liliana Ducatti Lopes Rubens Macedo Arantes Vinicius Rocha Santos Rodrigo bronze de Martino Luiz Augusto Carneiro D’a.b.querque Department of Gastroenterology Transplantation UnitUniversidade de São PauloSão PauloBrazil

background:perihilar cholangiocarcinoma(phCCC)is a dismal *** is no consensus regard-ing the best treatment for patients with unresectable *** present review aimed to gather the current pieces of evidence for liver transplantation and liver resection as a treatment for phCCC and to build better guidance for clinical *** sources:The search was conducted in pubMed,Embase,Cochrane,and *** related references were searched *** criteria were:reports in English or portuguese literature that a)patients with confirmed diagnosis of phCCC;b)patients treated with a curative intent;c)patients with the outcomes of liver resection and liver *** reports,reviews,letters,editorials,conference abstracts and papers with full-text unavailability were excluded from the ***:Most of the current literature is based on observational retrospective studies with low grades of *** resection has better long-term outcomes than systemic chemotherapy or palliation ther-apy and liver transplantation is a good alternative for selected patients with unresectable *** candidates for resection or transplantation should be medically fit and free of intrahepatic or extrahep-atic *** a general rule,patients presenting with a tumor having a longitudinal size>3 cm or extending below the cystic duct,lymph node disease,confirmed extrahepatic dissemination;intraoper-atively diagnosed metastatic disease;a history of other malignancies within the last five years,and did not complete chemoradiation regimen and were medically unfit should not be considered for *** of these criteria should be individually *** transplantation or resection should only be considered in highly experienced hepatobiliary centers,and any decision-making must be based on a multidisciplinary ***:phCCC is a complex condition with high *** therapies,including hepatec-tomy and liv

关键词： Liver transplantation Cholangiocarcinoma Liver neoplasms

Mulberry biomass-Derived Nanomedicines Mitigate Colitis through Improved Inflamed Mucosa Accumulation and Intestinal Microenvironment Modulation

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Research 2024年第1期2023卷 791-807页

作者： Wenjing Yang Ya Ma Haiting Xu Zhenhua Zhu Jiaxue Wu Cheng Xu Wei Sun Erhu Zhao Min Wang Rui L.reis Subhas C.Kundu Xiaoxiao Shi bo Xiao State Key Laboratory of Silkworm Genome biology College of SericultureTextileand Biomass SciencesSouthwest UniversityChongqing 400715China Chongqing Key Laboratory of Soft-Matter Material Chemistry and Function Manufacturing Faculty of Materials and EnergySouthwest UniversityChongqing 400715China Department of Gastroenterology The First Affiliated Hospital of Nanchang UniversityNanchang 330006China bs Research Group I3Bs—Research Institute on BiomaterialsBiodegradables and BiomimeticsUniversity of MinhoHeadquarters of the European Institute of Excellence on Tissue Engineering and Regenerative MedicineAveParkBarco 4805-017GuimaraesPortugal ICVS/3b’s-pT Government Associate Laboratory BragaGuimarãesPortugal

The therapeutic outcomes of conventional oral medications against ulcerative colitis(UC)are restricted by inefficient drug delivery to the colitis mucosa and weak capacity to modulate the inflammatory ***,a fluorinated pluronic(Fp127)was synthesized and employed to functionalize the surface of mulberry leaf-derived nanoparticles(MLNs)loading with resveratrol nanocrystals(RNs).The obtained Fp127@RN-MLNs possessed exosome-like morphologies,desirable particle sizes(around 171.4 nm),and negatively charged surfaces(−14.8 mV).The introduction of Fp127 to RN-MLNs greatly improved their stability in the colon and promoted their mucus infiltration and mucosal penetration capacities due to the unique fluorine effect.

关键词： fluorine sizes biomass

Rifaximin on epigenetics and autophagy in animal model of hepatocellular carcinoma secondary to metabolic-dysfunction associated steatotic liver disease

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World Journal of Hepatology 2024年第1期16卷 75-90页

作者： Matheus Truccolo Michalczuk Larisse Longo Melina.b.lén Keingeski bruno de Souza.b.sso Gabriel Tayguara Silveira Guerreiro Jessica T Ferrari JoséEduardo Vargas Cláudia p Oliveira Carolina Uribe-Cruz Carlos Thadeu Schmidt Cerski Eduardo Filippi-Chiela Mário reisÁlvares-da-Silva Division of Gastroenterology Hospital de Clínicas de Porto AlegrePorto Alegre 90035-903Rio Grande do SulBrazil Graduate program in Gastroenterology and Hepatology Universidade Federal do Rio Grande do SulPorto Alegre 90035-003Rio Grande do SulBrazil Experimental Laboratory of Hepatology and Gastroenterology Hospital de Clínicas de Porto AlegrePorto Alegre 90035-903Rio Grande do SulBrazil Laboratory of Inflammatory and Neoplastic Cells Universidade Federal do ParanáParaná81530900Brazil Department of Gastroenterology(LIM07) Faculdade de Medicina da Universidade de São PauloSão Paulo 01246903Brazil Facultad de Ciencias de la Salud Universidad Católica de las MisionesPosadasMisiones 3300Argentina Unit of Surgical pathology Hospital de Clinicas de Porto AlegrePorto Alegre 90035-903Rio Grande do SulBrazil Center of biotechnology Universidade Federal do Rio Grande do SulPorto Alegre 90035-003Rio Grande do SulBrazil Department of Morphological Sciences Universidade Federal do Rio Grande do SulPorto Alegre 90.050-170Rio Grande do SulBrazil Conselho Nacional de Desenvolvimento Científico e Tecnológico(CNpq) Conselho Nacional de Desenvolvimento Científico e Tecnológico(CNPq)ResearcherBrasília 71.605-001Brazil

bACKGROUND prevalence of hepatocellular carcinoma(HCC)is increasing,especially in patients with metabolic dysfunctionassociated steatotic liver disease(MASLD).AIM To investigate rifaximin(RIF)effects on epigenetic/autophagy markers in *** Adult Sprague-Dawley rats were randomly assigned(n=8,each)and treated from 5-16 wk:Control[standard diet,water plus gavage with vehicle(Veh)],HCC[high-fat choline deficient diet(HFCD),diethylnitrosamine(DEN)in drinking water and Veh gavage],and RIF[HFCD,DEN and RIF(50 mg/kg/d)gavage].Gene expression of epigenetic/autophagy markers and circulating miRNAs were *** All HCC and RIF animals developed metabolic-dysfunction associated steatohepatitis fibrosis,and cirrhosis,but three RIF-group did not develop *** animals who developed HCC with those who did not,miR-122,miR-34a,tubulin alpha-1c(Tuba-1c),metalloproteinases-2(Mmp2),and metalloproteinases-9(Mmp9)were significantly higher in the *** opposite occurred with becn1,coactivator associated arginine methyltransferase-1(Carm1),enhancer of zeste homolog-2(Ezh2),autophagy-related factor LC3a.b.Map1 Lc3b),and p62/sequestosome-1(p62/SQSTM1)-*** with controls,Map1 Lc3b,becn1 and Ezh2 were lower in HCC and RIF-groups(ppared to RIF(ppatic expression of Mmp9 was higher in HCC in relation to the control;the opposite was observed for p62/Sqstm1(ppression of p62/SQSTM1 protein was lower in the RIF-group compared to the control(p=0.024).There was no difference among groups for Tuba-1c,Aldolase-b,alpha-fetoprotein,and Mmp2(p>0.05).miR-122 was higher in HCC,and miR-34a in RIF compared to controls(pb was lower in HCC compared to RIF,and the inverse was observed for miR-224(pps regarding miR-33a,miR-143,miR-155,miR-375 and miR-21(p>0.05).CONCLUSION RIF might have a possible beneficial effect on preventing/delaying liver carcinogenesis throu

关键词： Animal model Autophagy Epigenetic Hepatocellular carcinoma Metabolic dysfunction-associated steatotic liver disease Rifaximin

A WW Domain-Containing protein Forms Immune Nuclear bodies against begomoviruses

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Molecular plant 2018年第12期11卷 1449-1465页

作者： lara p. Calil lana p.S. Quadros Thais C. Araujo Christiane E.M. Duarte bianca C. Gouveia-Mageste Jose Cleydson F. Silva Otavio J.b. brustolini Ruan M. Teixeira Caue N. Oliveira Rafael W.M.M. Milagres Gilberto S. Martins Joanne Chory p.dro a.b. reis Joao paulo b. Machado Elizabeth p.b. Fontes Departament of biochemistry and Molecular biology Universidade Federal de Vigosa Vigosa Minas Gerais 36570-000 Brazil Nati onal In stitute of Science and Tech no logy in plant-pest Interactions Bioagro Universidade Federal de Vicosa Vinosa Minas Gerais 36570-000 Brazil Departament of Genetics Universidade Federal do Rio de Janeiro Rio de Janeiro Rio de Janeiro Brazil Howard Hughes Medical Institute and plant biology Laboratory The Salk Institute of Biological Studies La Jolla CA 92037 USA Agronomy Institute Universidade Federal de Vigosa Campus Florestal Florestal Minas Gerais 35690-000 Brazil These authors contributed equally to this article

The bipartite begomoviruses (Geminiviridae family), which are DNA viruses that replicate in the nucleus of infected cells, encode the nuclear shuttle protein (NSp) to facilitate the translocation of viral DNA from the nucleus to the cytoplasm via nuclear pores. This intracellular trafficking of NSp-DNA complexes is accessorized by the NSp-interacting guanosine triphosphatase (NIG) at the cytosolic side. Here, we report the nuclear redistribution of NIG by AtWWpI, a WW domain-containing protein that forms immune nuclear bodies (Nbs) against begomoviruses. We demonstrated that AtWWpI relocates NIG from the cytoplasm to the nucleus where it is confined to AtWWp1-Nbs, suggesting that the NIG-AtWWp1 interaction may interfere with the NIG pro-viral function associated with its cytosolic localization. Consistent with this assumpti on joss AtWWpI functi on cuased plants more susceptible to begomovirus infection, whereas overexpression of AWWp1 enhaneed plant resistance to begomovirus. Furthermore, we found that a mutant versi on of AtWWpI defective for Nb formatio n was noIonger capable of interacti ng with and relocating NIG to the nucleus and lost its immune function against begomovirus. The antiviral function of AtWWp1-Nbs, however, could be antagonized by viral infection that induced either the disruption or a decrease in the number of AtWWp1-Nbs. Collectively, these results led us to propose that AtWWpI organizes nuclear structures into nuclear foci, which provide intrinsic immunity against begomovirus infection.

关键词： nuclear bodies antiviral immunity begomovirus NIG AtWWp1

Analyzing the economic viability of a large-scale integrated model of municipal solid waste:a study case for the most important brazilian economic region

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Waste Disposal and Sustainable Energy 2020年第3期2卷 231-247页

作者： Octavio pimenta reis Neto Mechanical Engineering Faculty at State University of Campinas(pDSE/FEM/UNICAMp) Av.Mofarrej275-Apto 212 B1São PauloSP CEP 05311-000Brazil

Despite the National policy for Solid Waste(pNRS)in 2010,nothing has changed to waste disposal in *** to reach 100%of all Municipal Solid Waste(MSW)collected and treated in landfills by Aug.2nd,2014,until nowadays,42%of this total remains in *** the most important national economic region treating its urban waste in landfills,what it has is no more than 4%of recycling and its landfills reaching the *** other ones is getting harder year by year,due to water reservoirs around the region,high freight costs,waste disposal and the severe control of emissions associated with its *** article comes to break the paradigm of investment and profitability proposing an alternative to the land-use,achieving higher rates of *** economic viability,carried out through well-known financial variables and Monte Carlo analysis,has taken into account proven local waste characteristics and market prices for recyclables and energy without *** considered a proposal highly intensive in capital and people,the revenues from the sales would be enough to guarantee the viability of 100%equity with IRR of 33.7%and ROI of 24.5%per year within the confidence of 99%.

关键词： Solid waste Sorting Recycling Waste-to-energy Metropolitan Region of São paulo Energy recovering