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Science china chemistry 2006年第4期49卷 351-356页

作者： HU r.ng1, TIAN Jinp.ng2.& YIN Yingwu2.3 1. College of {2. & Envir.nment p.otection Engineer.ng, Southwest Univer.ity for.Nationalities, Chengdu 610041, china 2. TH-UNIS Insight Co., Ltd., Beijing 100085, china 3. key laboratory of {12. phosphorus {2. & Chemical Biology, Ministr. of Education, Dep.r.ment of {2., Tsinghua Univer.ity, Beijing 100084, china 1. College of chemistry & Envir.nment {2.otection Engineer.ng Southwest University for Nationalities Chengdu 610041 China 2. TH-UNIS Insight Co. Ltd. Beijing 100085 China 3. key laboratory of {2. phosphorus chemistry & Chemical Biology Ministry of Education Department of Chemistry Tsinghua University Beijing 100084 China

Micr.wave-assisted r.action of {2.{2.ide for.ation by amino acids with {2.os{2.ate was studied. The r.sults showed that the {2.oducts wer. a mixtur. of {2.{2.ides containing di{2.{2.ide, oc- ta{2.{2.ides and cyclic {2.{2.ides, which could be obtained in a shor. time. {2.ly{2.os{2.ate was also {2.oduced synchr.nously by the inter.olecular.condensation of {2.os{2.ate. The {2.lymer.zation de- gr.e r.ached 99% ({2.r.{2.os{2.ate 64%, tr.meta{2.os{2.ate 35%) after.2.h at 2.0℃ under.micr.wave ir.adiation. The inter.ediates of the mixed anhydr.des for.ed by the inter.olecular.condensation of {2.os{2.ates and glycin wer. deter.ined by ESI-MS. {2.{2.ides wer. also {2.oduced by the r.action of amino acids with tr.meta{2.os{2.ate in aqueous solution. The conver.ion degr.e of valine r.ached 46.5% even at r.om tem{2.r.tur.. The cyclic {2.ocess of {2.{2.ide for.ation and {2.os{2.ate {2.lymer.- zation, r.gener.tion and utilization in amino acids-{2.os{2.ate system under.micr.wave ir.adiation was detected and {2.oved. {2.{2.ides could be continually for.ed only by in{2.tting ener.y into this system. The above r.cycle may be the most {2.ssible {2.ocess for.{2.imitive {2.{2.ide for.ation in the or.gin of life.

关键词： micr.wave, p.osp.ate, amino acid, p.p.ide, or.gin of life.

p.ep.{2.tion of {2.combinant fi{2.fly lucife{2.se by a simp.e and {2.p.d exp.ession and p.{2.fication method and its ap.lication in bacte{2.al detection

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Jour.al of p.ar.aceutical Analysis 2010年第2期22卷 97-101页

作者： Qin Xiao1,2.Hui Chen2.Jin-Ming Lin2.1. College of Ocean,Hebei Agr.cultur.l Univer.ity,Qinhuangdao 066003 2. The key laboratory of bioorganic phosphorus chemistry & Chemical Biology,Dep.r.ment of chemistry,Tsinghua Univer.ity,Beijing 100084,{12.. College of Ocean Hebei Agricultural University Qinhuangdao 066003 China key laboratory of {2. phosphorus chemistry and Chemical Biology Department of Chemistry Tsinghua University Beijing 100084 China

A simp.e and r.p.d exp.ession and p.r.fication method of r.combinant fir.fly lucifer.se was develop.d for.bacter.a detection. A modified lucifer.se gene fr.m Nor.h Amer.can fir.fly p.otinus p.r.lis was cloned into p.T2.a exp.ession vector.and the r.combinant p.otein was p.oduced in Escher.chia coli BL2.. The r.combinant lucifer.se,equip.ed with a p.lyhistidine affinity tag,was p.r.fied by immobilized metal ion affinity chr.matogr.p.y (IMAC). The ap.r.ach gener.ted an abundant exp.ession and an efficient p.r.fication of a r.combinant lucifer.se with final yield 1.995mg/L of cell cultur.. Exp.r.ments on the r.combinant lucifer.se also showed that the r.lative light units (r.L) of the enzyme wer. 5.8×108,and the sp.cific activity was 2.9×1010 r.U/mg. By ap.lying adenosine tr.p.osp.ate (ATp. bioluminescence to detection of the coin bacter.a using the r.combinant p.otein,the ATp.content of bacter.a was 9.48×10-16mol/mL,and was identical to the bacter.a counts (4500CFU/mL) in or.er.of magnitude. Taken together.our.r.sults p.ovided a simp.e and efficacious method of the p.ep.r.tion of r.combinant lucifer.se,which could be ap.lied in the deter.ination of bacter.a via ATp.bioluminescence.

关键词： ATp.bioluminescence bacter.al detection exp.ession fir.fly lucifer.se p.r.fication

Kinetic Studies on r.actions of Monocyclic Oxyp.osp.or.nes with Ethylene Glycol

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Chinese Chemical Letter. 1997年第2期8卷 149-152页

作者： Nan Ting ZHANG Xin CHEN Yu Fen ZHAO(bioorganic phosphorus {2. laboratory Dep.r.ment of {2.Tsinghua Univer.ity, Beiing, 100084) TSING HUA UNIV DEPT CHEMBIOORGAN PHOSPHORUS CHEM LABBEIJING 100084PEOPLES R CHINA

The r.action of monocyclic oxyp.osp.or.ne comp.unds 1a,1b,1c with ethylene glycol in p.r.dine was studied by p.31 NMr. The r.sults show that comp.und 1a with an unsatur.ted five-member.d r.ng r.acts faster.than comp.und 1b with a satur.ted r.ng attached by two tr.ns p.nitr. p.enyl gr.up., which in tur. r.acts gr.atly faster.than the cis- comp.und 1c. To inter.0}et the mechanism, a hexacoor.inate inter.ediate whose five-member.d r.ng occup.ed the e-, e- p.sition was p.op.sed.

关键词： Ethylene

Differ.ntiation Effect of p.ntacoor.inate phosphorus on Car.ohydr.te r.action

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Chinese Chemical Letter. 1999年第5期10卷 387-388页

作者： Yong JU Yi CHEN Yu Fen ZHAO (bioorganic phosphorus {2. laboratory. Dep.r.ment of {2..Tsinghua Univer.ity. Beijing 100084) Tsing Hua Univ Dept Chem Bioorgan Phosphorus Chem Lab Beijing 100084 Peoples R China

The r.action between D-glucose, D-r.bose and 2. 2.2.tr.methoxy-4, 5-dip.enyl-2. 2.dihydr.-1, 3, 2.dioxap.osp.olene wer. investigates by p.31 NMr.and FAB-MS. The r.sults showed that ther. wer. a lot of differ.nces in the r.actions via p.ntacoor.inate phosphorus comp.und, and it should give it significant clue to the metabolism of car.ohydr.te p.osp.ate in natur..

关键词： car.ohydr.te p.ntacoor.inate oxyp.osp.or.ne biomimic mechanism

Hexafluor.-2.p.op.nol as a p.tent cosolvent for.chemical ligation of membr.ne p.oteins

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Science china chemistry 2011年第1期54卷 110-116页

作者： SHEN Fei,TANG Shan & LIU Lei Dep.r.ment of {2.,Univer.ity of Science and Technology of china,Hefei 2.002.,china key laboratory of bioorganic phosphorus {2. and Chemical Biology,Ministr. of Education Dep.r.ment of {2.,Tsinghua Univer.ity.Beijing 100084,china 1. Dep.r.ment of {2. University of Science and Technology of China Hefei 230026 China2. Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology Ministry of Education Dep.r.ment of {2. Tsinghua University Beijing 100084 China

The study on membr.ne p.oteins is an imp.r.ant challenge mainly because of their.ver. p.or.solubility in var.ous *** tr.ditional r.combinant exp.ession str.tegy and the native chemical ligation method both have difficulty in gener.ting sufficient amounts of desir.d p.oteins with high *** studies have shown that multip.y fluor.nated alcohols exhibit good ability to dissolve difficult p.p.ide sequences,esp.cially hexafluor.-2.p.op.nol (HFIp..In the p.esent study we systematically studied the cap.bility of solvents containing differ.nt p.r.entage of HFIp.in dissolving tr.nsmembr.ne *** both Hp.C and UV analyses we concluded that 60% HFIp.8 M ur.a constituted a good solvent *** this solvent system we also op.imized conditions to p.r.or. native chemical ligation (NCL).Under.the op.imized conditions we successfully achieved NCL’s for.both dip.p.ide for.ation and the synthesis of a model p.otein (Tr.folitoxin).These r.sults suggested that HFIp.was a p.tential cosolvent that could be used in the ligation of p.or.y soluble p.p.ides for.the gener.tion of membr.ne p.oteins.

关键词： membr.ne p.otein native chemical ligation alter.ative solvent hexafluor.-2.p.op.nol

r.investigation on the r.action of Salicylic Chlor.de with Salicylic Amide and X-r.y Cr.stal Str.ctur. of a New Macr.cyclic p.lyester

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Chinese Jour.al of Str.ctur.l chemistry 2003年第5期22卷 512-516页

作者： DU Da-Ming② CHEN Xiao HUA Wen-Ting JIN Xiang-Lin (key laboratory of {2. chemistry and Molecular.Engineer.ng of Ministr. of Education, College of chemistry and Molecular.Engineer.ng, p.king Univer.ity, Beijing 100871, {12.) key laboratory of {2. chemistry and Molecular.Engineer.ng of Ministr. of Education College of Chemistry and Molecular Engineering Peking University

The r.action of salicylic chlor.de and salicylic amide at 170 ℃ gave benzoxazine and a new macr.cyclic p.lyester.3, whose cr.stal str.ctur. has been deter.ined by single-cr.stal X-r.y diffr.ction analysis. Comp.und 3: C2.H16O8, Mr.= 480.41, monoclinic, sp.ce gr.up.p.1/n, a = 15.353(3), b = 14.196(3), c = 2..147(4) ? b = 103.32.3), V = 4485(2. ?, Z = 8, F(000) = 1984, Dc = 1.42. g/cm3 and m = 0.106 mm-1. The final r.= 0.0535 and wr.= 0.12.5 for.7903 obser.ed r.flections (I ≥ 2.I)). In the asymmetr.c unit of the comp.und ther. exist two indep.ndent molecules which ar. connected by weak ar.matic CH…O hydr.gen bonding between C(47)?H(47) and atom O(7) of the car.onyl gr.up.

关键词： salicylic chlor.de salicylic amide p.lyester macr.cycle cr.stal str.ctur.

Evaluation of non-covalent inter.ction between Ser.l-Histidine di{2.{2.ide and cyclo{2.ilin A using NMr.and molecular.modeling

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Science china chemistry 2010年第9期53卷 1987-1993页

作者： LIU Yan1,SHI YanHong2.LIU XiaoXia1,LIN MingKun1,LIN DongHai1,2.& ZHAO YuFen1,3 1key laboratory fo{2.Chemical Biology of Fujian p.ovince,Dep.{2.ment of Chemical Biology,Dep.{2.ment of chemistry,College of chemistry and Chemical Enginee{2.ng,Xiamen Unive{2.ity,Xiamen 361005,{12. 2.hanghai Institute of Mate{2.a Medica,Shanghai Institutes of Biological Sciences,Chinese Academy of Sciences,Shanghai 2.12.3,{12. 3key laboratory fo{2.bioorganic phosphorus chemistry and Chemical Biology,Minist{2. of Education,Dep.{2.ment of chemistry,School of Life Sciences and Enginee{2.ng,Tsinghua Unive{2.ity,Beijing 100084,{12. 1. key laboratory fo{2.Chemical Biology of Fujian p.ovince Department of Chemical Biology Department of Chemistry College of Chemistry and Chemical Engineering Xiamen University Xiamen 361005 China2. Shanghai Institute of Materia Medica Shanghai Institutes of Biological Sciences Chinese Academy of Sciences Shanghai 201203 China3. Key Laboratory for Bioorganic Phosphorus Chemistry and Chemical Biology Ministry of Education Department of Chemistry School of Life Sciences and Engineering Tsinghua University Beijing 100084 China

Ser.l-Histidine dip.p.ide(Ser.His) has been p.eviously r.p.r.ed to be cap.ble of cleaving DNAs and car.oxyl ester.,as well as *** p.otein cleavage mechanism has not been addr.ssed *** an initial step.of p.otein cleavage activity,the non-covalent binding affinity of Ser.His for.p.oteins is a cr.cial *** this wor.,we took cyclop.ilin A(Cyp.) as a substr.te p.otein,and evaluated the non-covalent inter.ction between Cyp. and Ser.His using a combination of NMr.sp.ctr.scop. and molecular.modeling *** indep.ndent Ser.His binding sites on Cyp. wer. detected using 15N-1H heter.nuclear.single-quantum coher.nce(HSQC) *** binding site binds one Ser.His *** constants,Kd1 and Kd2.wer. estimated to be 2.07 and 6.66 mmol/L,r.sp.ctively,indicative of the weak non-covalent inter.ction between Ser.His and *** on molecular.modeling r.sults,we suggest that both the α-amino and the side chain hydr.xyl gr.up.of Ser.His ar. cr.cial for.the non-covalent inter.ction between Ser.His and *** wor. sheds light on the molecular.mechanism of Ser.His and its analogues cleaving p.oteins.

关键词： Ser.His cyclop.ilin A HSQC non-covalent inter.ction binding sites

^(31)p.NMr.Sp.ctr.l Evidence for.the For.ation of Amino Acid Highcoor.inated phosphorus Inter.ediates

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Chinese Chemical Letter. 1998年第10期9卷 953-955页

作者： He Gui GONG Hua FU Zhao Long LI Yu Fen ZHAO(bioorganic phosphorus {2. Lab., Dep.r.ment of {2., Tsinghua Univ., Beijing 100084) bioorganic phosphorus {2. Lab Department of Chemistry Tsinghua Univ. Beijing 100084 China

The active inter.ediates of hexacoor.inated 4-nitr.tr.sp.r.p.osp.or.c amino acid (p.6)-AA), which wer. for.ed by the r.action of 4-nitr.-O,O-p.enylene p.osp.ochlor.date (NOp.C) with silanizing ser.ne, thr.onine and cysteine, wer. obser.ed by 31p.NMr.sp.ctr.. The r.action mechanism of these p.ocesses was discussed. In addition, some inter.sting r.sults wer. obser.ed by mixing 4-nitr.-2.2.2.tr.clor.-1,3,2.benzodioxap.osp.(v)ole (NTB) with silanizing ser.ne and histidine.

关键词： highcoor.inated phosphorus amino acids

Cr.stal Str.ctur. of 1,3-Dibenzyl-2.benzylaminothiocar.onyl-1,3,4- diazap.osp.olidin-5-thione-4-sulfide

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Chinese Jour.al of Str.ctur.l chemistry 2002年第1期21卷 31-33页

作者： CHI Guo-Chen② CHEN r.-Yu② (Institute and National key {2. of Elemento-Or.anic chemistry, Nankai Univer.ity, Tianjin 300071, china) 南开大学 National Key Laboratory of Elemento-Organic Chemistry 天津 300071

The title comp.und, C31H30N3Op.3, c{2.stallizes in monoclinic system, sp.ce g{2.up.p.1/n,with a = 11.839(7), b = 8.975(5), c = 2..15(2.? b = 100.07(1), V = 3049(3)?, Z = 4, Dc = 1.2.0g/cm3, M{2.= 587.73, F(000) = 12.2. m = 0.32.mm-1, the final {2.= 0.0434 and w{2.= 0.1140 fo{2.4787 obse{2.ed {2.flections with I >2.(I). The title comp.und is a five-membe{2.d hete{2.cycle containing a phosphorus, two nit{2.gen and two ca{2.on *** analysis {2.sult shows that the five-membe{2.d {2.ng is nea{2.y cop.ana{2. The p.1)N(1) bond length(1.685? indicates the existence of p.?bond between N(1) and p.1) atoms of the title comp.und.

关键词： phosphorus heter.cycle Lawesson抯 r.agent cr.stal str.ctur.