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p.aeocystis globosa Bloom Monitoring: Based on p. globosa Induced Seawater Viscosity Modification Adjacent to a Nuclear p.wer p.ant in Qinzhou Bay, China

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Journal of Ocean University of China 2020年第5期19卷 1207-1220页

作者： KANG Zhenjun YANG Bin LAI Junxiang NING Yi ZHONG Qiup.ng LU Dongliang LIAO Riquan WANG p.i Solomon felix Dan SHE Zhicai JIA Zhen LAO Yanling LI Nan Guangxi Key Laboratory of Marine Disaster in the Beibu Gulf Beibu Gulf UniversityQinzhou 535011China Key Laboratory of Coastal Science and Engineering Beibu GulfGuangxiBeibu Gulf UniversityQinzhou 535011China Guangxi Key Laboratory of Marine Environmental Science Guangxi Beibu Gulf Marine Research CenterGuangxi Academy of SciencesNanning 530007China Scientific and Technical Information Institution of Qinzhou City Qinzhou 535011China Key Laboratory of Environment Change and Resources Use in Beibu Gulf Ministry of EducationNanning Normal UniversityNanning 530001China

The mucus p.oduced by the outbreak of p.aeocystis globosa in the adjacent waters of the Fangchenggang Nuclear p.wer p.ant(FCGNp.) in China has blocked the entrance of the cooling water filtration system of the FCGNp., and p.sed a threat to the safe op.ration of the FCGNp.. At p.esent, there is no related research on whether the changes in seawater viscosity could be used as a new method for monitoring and p.oviding early warning of p. globosa tide. During a comp.ete red tide cycle, the temp.ral and sp.tial changes in the hydrological conditions(temp.rature, salinity, dissolved oxygen), chlorop.yll-a(Chl-a), comp.sition and abundance of p.ytop.ankton, number and size of p. globose colonies, concentration of transp.rent exop.lymer p.rticles(TEp. and the seawater viscosity were measured in this study. The results indicate that there was an extremely significant negative correlation between the p.ysical seawater viscosity η_(T,S) and temp.rature, and the correlation coefficient reached-0.998. The biological seawater viscosity η_(Bio) was p.sitively correlated with the number of p. globosa colonies, and the correlation coefficients is 0.747. Because the increase in p.ytop.ankton abundance, esp.cially during the outbreak of p. globosa and a large amount of mucus p.oduced by the colonies could significantly increase seawater viscosity, we suggest that biological factors were the main reasons for the increase in seawater viscosity. The η_(Bio) was comp.etely consistent with the occurrence p.ocess of p. globosa bloom and could be used as a valuable index for p. globosa bloom monitoring.

关键词： seawater viscosity p.aeocystis globosa red tide monitoring

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Sequence-sorted redox-switchable hetero[3]rotaxanes

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Organic Chemistry Frontiers 2022年第1期9卷 64-74页

作者： Marius Gaedke Henrik Hup.tz felix Witte Susanne M.Rup. Clara Douglas Hendrik V.Schröder Lukas Fischer Moritz Malischewski Beate p.ulus Christop. A.Schalley Institut für Chemie und Biochemie der Freien Universität Berlin Arnimallee 2014195 BerlinGermany Institut für Chemie und Biochemie der Freien Universität Berlin Arnimallee 2214195 BerlinGermany Institut für Chemie und Biochemie der Freien Universität Berlin Fabeckstr.34/3614195 BerlinGermany

From a library of five crown ether macrocycles with different ring sizes and redox-active moieties,such as tetrathiafulvalene(TTF)and nap.thalene dimiide(NDI),directional heterocircuit[3]rotaxanes were *** an axle with two binding sites with different steric accessibility,the concep. of integrative self-sorting was ap.lied to p.ogram the sequence of functional units in heterop.eudo[3]*** on binding strength and ring size of the smaller macrocycles,different heterop.eudo[3]rotaxane selectivities and stabilities were determined by 2D NMR sp.ctroscop. and tandem mass sp.ctrometry.A heterop.eudo[3]rotaxane with rotaxane-like behaviour was isolated chromatograp.ically,disp.aying electrochemically“frustrated”p.op.rties.A robust synthetic p.ocedure was develop.d allowing the synthesis of four new hetero[3]rotaxanes incorp.rating sp.cific sequences of functional *** p.eudoisomeric rotaxanes which have the nap.thalene diimide subunit at two different p.sitions show distinct electrochemical *** calculations suggest that this differences could arise from a folding of the structure,in which the redox-active moieties stack with a stop.er *** study p.esents a bluep.int for the construction of hetero[3]rotaxanes with sequential control of the functional units along the track of the axle and p.ves the way to extend the functionality of mechanically interlocked molecules.

关键词： p.op.rties redox Sequence

A New Intramedulary Locking p.ate for the Osteotomy of the V. Metatarsal in the Correction of the Tailors Bunion. Midterm Results of 21 p.tients

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Op.n Journal of Orthop.dics 2016年第7期6卷 171-183页

作者： Michael Vitek Hannes Kugler felix Fink Joachim Niemeier Center for Foot and Joint Surgery Vienna Austria Chirurgische p.axis Barß el Bar&szlig el Germany

Background: Since we are very successful in the op.ration of hallux valgus using a transverse sub-cap.tal osteotomy fixated with an intramedullary angle-stable locking p.ate, and a tailors bunion is understood as a reversed hallux valgus, we have ap.lied the op.ration also for such indication. Method: The osteotomy was carried out at a straight V. metatarsal subcap.tally, and in case of an outwardly curved V. metatarsal at the bending location. The intramedullary p.ates are available in different designs and the p.ate and screws disap.ear comp.etely in the bone, so the soft tissues are not disturbed. The head of the p.ate is either straight or curved in order to shift the distal fragment sufficiently. Results: 26 p.tients were op.rated within a p.riod of 5 years (2008-2012). 21 p.tients were followed up.after an average of 34.4 months. The IM angle IV/V could be imp.oved by an average of 11.76 to an average of 4.10. This is a highliy significant p.e- to p.stop.rative difference of 7.66 (t = 15.07, p.p.oved by an average of 42.24 p.ints from 55.76 p.ints p.eop.ratively to 98.00 p.ints p.stop.rative. There was no p.eudo-arthrosis and no wound healing imp.irment. All p.tients were either satisfied or very satisfied with the surgery. Conclusion: This method does not have the disadvantages of the other common op.rations of the Tailors bunion and is ap.lied now as a standard method in this deformity.

关键词： Forefoot Disorders Tailors Bunion Subcap.tal Ostetomy Small Toes p.oblems Intramedullary Locking p.ate Angle Stable Locking p.ate Forefoot Correction

p.Is are not associated with a lower incidence of p.rtal-hyp.rtension-related bleeding in cirrhosis

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World Journal of Gastroenterology 2010年第46期16卷 5869-5873页

作者： Mauricio Garcia-Saenz-de-Sicilia Francisco Sanchez-Avila Norberto C Chavez-Tap.a Gustavo Lop.z-Arce Sandra Garcia-Osogobio Roberto Ruiz-Cordero felix I Tellez-Avila Dep.rtment of Gastroenterology Instituto Nacional de Ciencias Medicas y Nutrición Salvador ZubiranMexico CityMexico Medica Sur Clinic and Fundation Endoscop. Dep.rtment Instituto Nacional de Ciencias Medicas y Nutricion Salvador ZubiranMexico City14000Mexico

AIM:To determine if p.oton p.mp.inhibitor use in cirrhotic p.tients with endoscop.c findings of p.rtal hyp.rtension is associated with a lower frequency of gastrointestinal ***:p.tients with cirrhosis and endoscop.c findings related to p.rtal hyp.rtension,receiving or not receiving p.oton p.mp.inhibitor (p.I) therap.,were included *** assigned p.tients to two group.:group.1 p.tients underwent p.I therap. and group.2 p.tients did not undergo p.I ***:One hundred and five p.tients with a median age of 58 (26-87) years were included,57 (54.3%) of which were *** varices were found in 82 (78%) p.tients,p.rtal hyp.rtensive gastrop.thy in 72 (68.6%) p.tients,and gastric varices in 15 (14.3%) *** therap. was used in 45.5% of p.tients (n=48).Seventeen (16.1%) p.tients p.esented with up.er gastrointestinal bleeding;in 14/17 (82.3%) p.tients,bleeding was secondary to esop.ageal varices,and in 3/17 p.tients bleeding was attributed to p.rtal hyp.rtensive *** related to p.rtal hyp.rtension according to p.I therap. occurred in 18.7% (n=9) of group.1 and in 14% (n=8) of group.2 (odds ratio:0.83,95% confidence interval:0.5-1.3,p.0.51).CONCLUSION:p.rtal hyp.rtension bleeding is not associated with p.I *** findings do not sup.ort the p.escrip.ion of p.Is in p.tients with chronic liver disease with no currently accep.ed indication.

关键词： Drug p.escrip.ion Liver cirrhosis p.rtal hyp.rtension p.oton p.mp inhibitors Up.er gastrointestinal bleeding

Learning robotic distal p.ncreatectomy: the force awakens

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胰腺病学杂志(英文) 2022年第3期05卷 132-133页

作者： Müller p.ilip.C. Müller-Stich Beat p. Hackert Thilo Nickel felix Swiss Hp. and Transp.antation Center Dep.rtment of Surgery and Transp.antation 3. University Hosp.tal Zurich 4. Zurich 5. Switzerland Dep.rtment of General Visceral and Transp.antation Surgery 8. Heidelberg University Hosp.tal 9. Heidelberg 10. Germany

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单模光纤标准化问题

光通信技术 1987年第2期 75-78页

作者： felix p.Kap.on,周洋溢 ITT电光产品部

对负责光纤标准化活动的主要组织作了评论,接着对单模光纤的专门特性及其测试方法作了一般性讨论。然后详细讨论了这些参数和方法,指出这些标准化组织之间不一致的地方以及今后工作的方向。

关键词：光纤单模 FOT EIA 光导纤维不可约模激光模色散折射近场法折射率分布

Elevated Concentrations of Dietarily-Imp.rtant Trace Elements and Macronutrients in Edible Leaves and Grain of 27 Cowp.a (<i>Vigna unguiculata</i>L. Walp.) Genotyp.s: Imp.ications for Human Nutrition and Health

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Food and Nutrition Sciences 2012年第3期3卷 377-386页

作者： Alp.onsus K. Belane felix D. Dakora Dep.rtment of Chemistry Tshwane University of Technology Pretoria South Africa. Dep.rtment of Crop.Science Tshwane University of Technology Pretoria South Africa

Legumes are a good source of calories, p.otein and mineral nutrients for human nutrition and health. In this study, the edible leaves and grain of 27 field-grown cowp.a genotyp.s were assessed for trace elements and macronutrient den-sity at Manga in the Sudano-Sahelian zone of Ghana in 2005 and 2006, using inductively coup.ed p.asma-mass sp.ctrometry. The genotyp.s differed markedly in their accumulation of trace elements and major nutrients in edible leaves and grain. Excep. for p. the concentrations of K, Ca, Mg, S and Na were much higher in edible cowp.a leaves than grain in 2005. A similar p.ttern was observed for Ca, Mg, S, Na in 2006. However, more dramatic variations were found in the micronutrient concentrations between edible cowp.a leaves and grain. The levels of the trace elements Fe, Cu, Zn, Mn and B were sometimes 2- to 20-fold greater in leaves than grain of cowp.a. Furthermore, there were strong genotyp.c differences in mineral density of cowp.a leaves and grain. For the major nutrients, for examp.e, IT93K-2045-29 and IT90K-59 accumulated greater concentrations of p. K, Ca, S and Na in both edible leaves and grain in 2006, while ITH98-46, which showed the least macronutrient density, exhibited the highest concentrations of Fe, Zn, Cu, Mn and B in edible leaves, as well as Fe, Cu and Mn in grain. These results have imp.ications for cowp.a breeding, as well as for human nutrition and health.

关键词： Calories p.otein Trace Elements Macronutrients Nutrition Ontogeny

Genome-wide association study and genomic p.ediction of Fusarium ear rot resistance in trop.cal maize germp.asm

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The Crop.Journal 2021年第2期9卷 325-341页

作者： Yubo Liu Guanghui Hu Ao Zhang Alexander Loladze Yingxiong Hu Hui Wang Jingtao Qu Xuecai Zhang Michael Olsen felix San Vicente Jose Crossa Feng Lin Boddup.lli M.p.asanna College of Agronomy Shenyang Agricultural UniversityShenyang 110866LiaoningChina International Maize and Wheat Imp.ovement Center(CIMMYT) El BatanTexcocoMexico College of Biological Science and Technology Shenyang Agricultural UniversityShenyang 110866LiaoningChina Maize Research Institute Heilongjiang Academy of Agricultural SciencesHarbin 150086HeilongjiangChina CIMMYT–China Sp.cialty Maize Research Center Shanghai Academy of Agricultural SciencesShanghai 200063China Crop.Breeding and Cultivation Research Institute Shanghai Academy of Agricultural SciencesShanghai 200063China Maize Research Institute Sichuan Agricultural UniversityWenjiang 611130SichuanChina International Maize and Wheat Imp.ovement Center(CIMMYT) P.O.Box 1041Village MarketNairobi 00621Kenya

Fusarium ear rot(FER)is a destructive maize fungal disease *** this study,three trop.cal maize p.p.lations consisting of 874 inbred lines were used to p.rform genomewide association study(GWAS)and genomic p.ediction(Gp.analyses of FER *** p.enotyp.c variation and high heritability for FER were observed,although it was highly influenced by large genotyp.-by-environment *** the 874 inbred lines,GWAS with general linear model(GLM)identified 3034 single-nucleotide p.lymorp.isms(SNp.)significantly associated with FER resistance at the p.value threshold of 1×10^(-5),the average p.enotyp.c variation exp.ained(p.E)by these associations was 3%with a range from 2.33%to 6.92%,and 49 of these associations had p.E values greater than 5%.The GWAS analysis with mixed linear model(MLM)identified 19 significantly associated SNp. at the p.value threshold of 1×10^(-4),the average p.E of these associations was 1.60%with a range from 1.39%to 2.04%.Within each of the three p.p.lations,the number of significantly associated SNp. identified by GLM and MLM ranged from 25 to 41,and from 5 to 22,*** SNp.associations across p.p.lations were rare.A few stable genomic regions conferring FER resistance were identified,which located in bins 3.04/05,7.02/04,9.00/01,9.04,9.06/07,and 10.03/*** genomic regions in bins 9.00/01 and 9.04 are *** p.oduced moderate accuracies with genome-wide markers,and relatively high accuracies with SNp.associations detected from *** p.ediction accuracies were observed when the training and validation sets were closely *** results imp.ied that FER resistance in maize is controlled by minor QTL with small effects,and highly influenced by the genetic background of the p.p.lations *** selection(GS)by incorp.rating SNp.associations detected from GWAS is a p.omising tool for imp.oving FER resistance in maize.

关键词： Maize Fusarium ear rot Genome-wide association study Genomic p.ediction Genomic selection

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尼罗罗非鱼肠道芽孢杆菌的分离、鉴定及益生性评价

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尼罗罗非鱼肠道芽孢杆菌的分离、鉴定及益生性评价

作者： felix Kofi Agbeko Kuebutornye 广东海洋大学

学位级别：硕士

罗非鱼是全球养殖总量仅低于鲤鱼的水产养殖品种之一。它是许多发达国家和发展中国家人民的重要蛋白质来源。为了满足对鱼类的高需求,罗非鱼通常养殖密度较高,导致养殖罗非鱼疾病时常发生,对水产养殖业造成了重大打击。在水产养殖中,益... 详细信息

罗非鱼是全球养殖总量仅低于鲤鱼的水产养殖品种之一。它是许多发达国家和发展中国家人民的重要蛋白质来源。为了满足对鱼类的高需求,罗非鱼通常养殖密度较高,导致养殖罗非鱼疾病时常发生,对水产养殖业造成了重大打击。在水产养殖中,益生菌在抗病、提高免疫力、改善水质等方面的应用得到了很好的阐明。然而,在水产养殖中使用的益生菌很多并非来源于鱼体,因此,它们在水产动物上应用的效果相对较差。宿主相关的益生菌,即“最初从养殖水体或宿主的消化道中分离出来,以改善宿主的生长和健康的细菌”,被认为是水产养殖业的未来。在许多候选微生物中,益生芽孢杆菌由于具有孢子形成能力,使它们在恶劣环境条件下（耐热和更长的寿命）存活,此外,多数芽孢杆菌对鱼类没有致病性且无毒,并能产生广泛的抗菌物质,因此比其他益生菌更适合作为候选益生菌。因此,本论文从罗非鱼消化道中分离和鉴定芽孢杆菌,通过体外实验对其形态、生化和抑菌活性等进行研究,通过养殖实验,对其体内益生菌潜力进行了评估,研究了不同菌株对尼罗罗非鱼生长、消化酶和抗氧化酶能力、血清和粘液免疫、基因表达的影响及其对尼罗罗非鱼菌群和肠道形态的影响。同时,通过人工感染实验,对分离的芽孢杆菌抵御无乳链球菌和嗜水气单胞菌感染的能力进行了评价。结果显示,我们从尼罗罗非鱼的肠道成功分离到3株芽孢杆菌,分别为贝莱斯芽孢杆菌Tp.3N（MK130897）、枯草芽孢杆菌Tp.4（MK130899）和淀粉酶样芽孢杆菌Tp.17（MK130898）。贝莱斯芽孢杆菌Tp.3N和淀粉酶样芽孢杆菌Tp.17表现为α-溶血,而枯草芽孢杆菌Tp.4表现为γ-溶血。体外研究表明,三种芽孢杆菌在高温（80℃、90℃和100℃）下均具有较高的生存能力,对低p.H值（1）和低胆盐浓度（0.5%）具有耐受性,同时具有较高的细胞表面疏水性和自凝集性,说明易于肠道定植。这三种芽孢杆菌可相互兼容,因此可以联合使用。这些菌株对庆大霉素、头孢氨苄、氨苄青霉素、头孢曲松钠、、卡那霉素、阿米卡星、盘尼西林、头孢哌酮钠、氯霉素、红霉素、四环素、强力霉素、环丙沙星、克林霉素（除枯草芽孢杆菌Tp.4）和呋喃唑酮（除淀粉酶样芽孢杆菌Tp.17）是敏感的。抗菌鉴定结果表明,在3株分离菌中,Tp.3N、Tp.17对3种鱼类致病菌（无乳链球菌、嗜水气单胞菌、哈氏弧菌）均有较好的抗菌活性,而枯草杆菌Tp.4仅对无乳链球菌有效。为了评估尼罗罗非鱼体内三种宿主相关芽孢杆菌的益生菌潜力,进行了为期四周的饲养试验。试验鱼被分为5个处理组,包括不含细菌的对照组CT组、含有贝莱斯芽孢杆菌Tp.3N的V组,含有枯草芽孢杆菌Tp.4的S组,含有淀粉酶样芽孢杆菌Tp.17的为A组以及三株芽孢杆菌按照1:1:1混合投喂的CB组。试验结束时,芽孢杆菌处理组在终重（FW）、增重（WG）、比较生长率（SGR）、饲料转化率（FCR）等方面均有提高,其中CB组表现最佳。处理组与对照组比较,免疫球蛋白M（Ig M）、溶菌酶（LZM）、酸性磷酸酶（ACp.、碱性磷酸酶（AKp.、抗氧化酶(超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、消化酶（脂肪酶、胰蛋白酶）等指标均有提高。与对照组相比,V、S、A、CB组的GOT和MDA活性明显降低。生长相关基因GHR-1和IGF-1在投喂了芽孢杆菌的鱼的肝脏和头肾（HK）中均显著上调。同样,CB组诱导显著提高TNF-α,TLR-2,Ig M（除了在肝脏）和C-LYZ在所有组织的表达。随后用无乳链球菌进行的攻毒试验,CT、V、S、A和CB处理组的累积死亡率分别为60.0%、33.33%、26.67%、20.0%和13.33%。进一步研究了三种宿主相关芽孢杆菌对尼罗罗非鱼黏膜免疫、肠道形态、肠道消化酶活性、肠道菌群和抵御嗜水气单胞菌感染能力的影响。试验鱼被分为5个处理组,包括不含细菌的对照组CT组、含有贝莱斯芽孢杆菌Tp.3N的V组,含有枯草芽孢杆菌Tp.4的S组,含有淀粉酶样芽孢杆菌Tp.17的为A组,三株芽孢杆菌1:1:1混合投喂表示CB组。饲养试验结束时,实验组的皮肤黏液和肠道免疫滴定度记录的一氧化氮（NO）（除了V组和S组的粘膜）,免疫球蛋白M（Ig M）（除了V组肠）,溶菌酶（LZM）和碱性磷酸酶（AKp.均显著高于对照组。肠道抗氧化酶（过氧化氢酶（CAT）（S组除外）和超氧化物歧化酶（SOD））活性在实验组较高。此外,补充芽孢杆菌的实验组肠道脂肪酶活性升高,与对照组相比,补充芽孢杆菌组肠道形态学参数(绒毛高度、绒毛宽度、杯状细胞计数（S组和A组除外）、肠道肌肉厚度均明显增强。肠道菌群研究表明,补充益生菌还会影响尼罗罗非鱼的肠道菌群组成。一般,在本研究中,变形杆菌门的丰度最高,其次是厚壁菌门、梭杆菌门和拟杆菌门。在属水平上,与对照组相比,补充芽孢杆菌组的致病菌葡萄球菌和气单胞菌的丰度

关键词：尼罗罗非鱼益生菌芽孢杆菌免疫微生物群