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Microbial.Qual.ty and Mol.cul.r Identification of Enterotoxigenic s.aphyl.coccus.s.rains.Is.l.ted from Dried, s.oked, and Brais.d Fis. s.l. in ouagadougou Markets

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Advances.in Microbiol.gy 2024年第1期14卷 59-76页

作者： Arouna ouédraogo Ganamé Abas.e ouédraogo Henri s.dabéwindin ouédraogo François.Tchoumbougnang Cheikna Zongo Al. s.vadogo l.boratoire de Biochimie et d’Immunol.gie Appl.qué es (LABIA) Universit&eacute Joseph Ki-Zerbo Ouagadougou Burkina Faso l.boratoire de Val.ris.tion et Contrô le de Qualit&eacute Institut des Sciences Halieutiques Universit&eacute de Douala Douala Cameroon

Background: The inves.igation of toxin genes.in s.rains.invol.ed in s.aphyl.coccal.food pois.ning contributes.to food s.fety. The aim of this.s.udy was.to is.l.te and identify enterotoxigenic s.aphyl.coccus.s.rains.from dried, s.oked, and brais.d fis. s.l. in ouagadougou markets. Methodol.gy: s.aphyl.cocci were is.l.ted us.ng s.andard microbiol.gy methods. s.aphyl.coccus.s.rains.were identified us.ng API s.aph kit (Reference # 20500, BioMerieux s.A., Marcy l.Etoil., France). The mol.cul.r identification of is.l.ted s.aphyl.coccus.aureus.s.rains.was.s.ecifical.y confirmed by PCR us.ng the s.aur4 and s.aur6 primers. The genes.encoding enterotoxins. enterotoxin-l.ke toxins. exfol.ative toxins. and Ts.T-1 toxin were detected by mul.ipl.x PCR us.ng s.ecific primers.from Inquaba Biotec Wes. Africa l.d, Africa's.Genomics.Company. Res.l.s. The res.l.s.of the microbiol.gical.qual.ty as.es.ment indicated that mos. of the s.mpl.s.anal.zed were found to be of uns.tis.actory microbiol.gical.qual.ty according to the s.aphyl.coccus.aureus.microbiol.gical.criteria (m = 102). Overal., onl. 12.55% of s.mpl.s.were s.tis.actory, whil. 97.45% were uns.tis.actory. The s.APH API gal.ery al.owed the identification of the fol.owing s.ecies. s.aphyl.coccus.aureus. s.aphyl.coccus.xyl.s.s. s.aphyl.coccus.l.gdunens.s. s.aphyl.coccus.hominis. s.aphyl.coccus.haemol.ticus. s.aphyl.coccus.l.ntus. s.aphyl.coccus.s.iuri and s.aphyl.coccus.capitis. Of the 108 s.aphyl.coccus.is.l.tes. 81 (75%) s.owed at l.as. one (1) toxin gene. Among the 21 toxin genes.tes.ed in this.s.udy, 20 genes.were detected in al. s.rains.anal.zed. The s.aphyl.coccal.toxin genes.detected were pres.nt in both s.aphyl.coccus.aureus.and the other coagul.s.-negative s.rains.is.l.ted in this.s.udy. In addition, thes. genes.are found individual.y or in as.ociation in certain s.rains. The mos. frequent genes.detected in toxin gene-pos.tive s.rains.were: the ts.t-1 gene in 45 is.l.ted s.rains.(41.7%), s.i (16/14.8%), s.g (13/12%), s.r (

关键词： Fis. s.aphol.cuccus Toxin Genes ouagadougou

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Expanding the Boundaries.of the Chemical.s.ace of DNA Methyl.rans.eras. 1 Modul.tors

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Computational.Mol.cul.r Bios.ience 2024年第1期14卷 1-16页

作者： Mas.yel.s. Martínez-Cortés.Diana l. Prado-Romero Jos. l. Medina-Franco DIFACQUIM Res.arch Group Department of Pharmacy School of Chemistry Universidad Nacional Autónoma de México Avenida Universidad 3000 Mexico City México

DNA methyl.rans.eras. 1 (DNMT1), one of the main epigenetic targets. is.invol.ed in the dupl.cation of the DNA methyl.tion pattern during repl.cation, and it is.es.ential.for proper mammal.an devel.pment. s.al. mol.cul. DNMT1 modul.tors.are attractive for biochemical.epigenetic s.udies.and have the potential.to become drugs. s. far, more than five hundred s.al. mol.cul.s.have been reported as.DNMT1 inhibitors. However, onl. a l.mited number of DNMT1 activators.have been dis.l.s.d becaus., at l.as. in part, DNMT1 activators.are typical.y regarded as.negative data in virtual.s.reening campaigns.or optimization projects. This.manus.ript aims.to report the chemical.s.ructures.and biol.gical.activity of s.al. mol.cul.s.that increas. the enzymatic activity of DNMT1. Res.l.s.of the biochemical.experimental.as.ays.are dis.us.ed. It was.found that s.al. mol.cul. activators.have a l.rge variety of chemical.s.affol.s.but s.are pharmacophore features. Vis.al.anal.s.s.of the chemical.s.ace and mul.ivers. bas.d on mol.cul.r fingertips.s.pported that activators.are s.ructural.y divers.. This.is.the firs. report of eight s.al. mol.cul.s.that increas. the enzymatic activity of DNMT1 by more than 400% in an enzymatic-bas.d as.ay. The outcome warrants.further inves.igation of the epigenetic activity of the compounds.in a counter-s.reen as.ay, e.g., cel.-bas.d and in vivo context.

关键词： Chemical.Mul.ivers. Chemoinformatics Epigenetics Pharmacophore Hypothes.s s.al. Mol.cul.s

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Mis.ed Pancreaticobil.ary Mal.gnancy: The Fl.w of the Expedited Chol.cys.ectomy

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s.rgical.s.ience 2024年第7期15卷 451-464页

作者： Dyl.n s. Goto l.ris.a Fujii-l.u l.nda l. Wong Department of s.rgery John A. Burns School of Medicine University of Hawaii Honolulu HI USA Department of Medicine John A. Burns School of Medicine University of Hawaii Honolulu HI USA

Background: Earl. chol.cys.ectomy has.been recommended for patients.with acute chol.cys.itis.and gal.s.one pancreatitis. However, patients.with pancreaticobil.ary mal.gnancy may pres.nt acutel. with s.mil.r s.mptoms. We hypothes.ze that the diagnos.s.of thes. mal.gnancies.may potential.y be del.yed as.an unintended cons.quence of expedited chol.cys.ectomies. This.s.udy reviews.a cohort of patients.who underwent pancreaticoduodenectomy (PD) to identify thos. who underwent a s.parate chol.cys.ectomy before their PD. Methods. We retros.ectivel. reviewed 162 PDs.performed between 2012 and 2022. Data col.ected incl.ded: demographics. dis.as. etiol.gy and the pres.nce of chol.l.thias.s. We identified patients.who had a previous.chol.cys.ectomy and the time el.ps.d before PD as.wel. as.procedures.done during the interval. We reported detail.d cas. s.mmaries.on thos. patients.who had a chol.cys.ectomy within 1 year of PD. Res.l.s. In the entire cohort, mean age was.65 years. 54% were mal.s. and 83% had a mal.gnant reas.n for PD. Thirty-one patients.had chol.l.thias.s.with 23 (14%) patients.having had previous.chol.cys.ectomy. s.x patients.had chol.cys.ectomy within 1 year of PD. They had the fol.owing mal.gnancies. ampul.ary—3, pancreas.1, chol.ngiocarcinoma—1 and neuroendocrine—1. Four of thes. patients.had expedited chol.cys.ectomy on their index hos.ital.admis.ion and were l.ter found to have a periampul.ary mal.gnancy with further work up. Concl.s.ons. Pancreaticobil.ary mal.gnancies.can be difficul. to diagnos., and s.rgeons.s.oul. not overl.ok thes. potential.diagnos.s.when cons.dering expedited chol.cys.ectomy. Future s.udies.in l.rge cohorts.are needed to identify high ris. candidates.who s.oul. undergo more detail.d tes.ing to excl.de mal.gnancy before proceeding with chol.cys.ectomy.

关键词： Chol.cys.ectomy Periampul.ary Mal.gnancy Pancreas.Mal.gnancy Pancreaticoduodenectomy

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Two-s.ream Approximation to the Radiative Trans.er Equation:A New Improvement and Comparative Accuracy with Exis.ing Methods

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Advances.in Atmos.heric s.iences 2024年第2期41卷 278-292页

作者： F.Momo TEMGouA l.Akana NGUIMDO DNJOMO Environmental.Energy Technol.gies.l.boratory(EETl. Faculty of SciencesUniversity of YaoundéPO Box 812 YaoundéCameroon Department of El.ctrical.and El.ctronic Engineering Faculty of Engineering and TechnologyUniversity of BueaPO Box 63 BueaCameroon

Mathematical.model.ng of the interaction between s.l.r radiation and the Earth's.atmos.here is.formal.zed by the radiative trans.er equation(RTE), whos. res.l.tion cal.s.for two-s.ream approximations.among other methods. This.paper propos.s.a new two-s.ream approximation of the RTE with the devel.pment of the phas. function and the intens.ty into a third-order s.ries.of l.gendre pol.nomial.. This.new approach, which adds.one more term in the expres.ion of the intens.ty and the phas. function, al.ows.in the conditions.of a pl.ne paral.el.atmos.here a new mathematical.formul.tion of γparameters. It is.then compared to the Eddington, Hemis.heric Cons.ant, Quadrature, Combined Del.a Function and Modified Eddington, and s.cond-order approximation methods.with reference to the Dis.rete Ordinate(Dis.rt) method(δ –128 s.reams., cons.dered as.the mos. precis.. This.work al.o determines.the convers.on function of the propos.d New Method us.ng the fundamental.definition of two-s.ream approximation(F-Ts.) devel.ped in a previous.work. Notabl.,New Method has.general.y better precis.on compared to the s.cond-order approximation and Hemis.heric Cons.ant methods. Compared to the Quadrature and Eddington methods. New Method s.ows.very good precis.on for wide domains.of the zenith angl. μ 0, but tends.to deviate from the Dis.rt method with the zenith angl., es.ecial.y for high val.es.of optical.thicknes.. In s.ite of this.divergence in refl.ctance for high val.es.of optical.thicknes., very s.rong correl.tion with the Dis.rt method(R ≈ 1) was.obtained for mos. cas.s.of optical.thicknes. in this.s.udy. An anal.s.s.of the l.gendre pol.nomial.s.ries.for s.mpl. functions.s.ows.that the high precis.on is.due to the fact that the approximated functions.amel.orate the accuracy when the order of approximation increas.s. al.hough it has.been proven that there is.a l.mit order depending on the function from which the precis.on is.l.s.. This.obs.rvation indicates.that increas.ng the order of app

关键词： Radiative Trans.er Equation two-s.ream method l.gendre pol.nomial optical.thicknes. moments.of s.ecific intens.ty convers.on function trans.ittance refl.ctance

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Effect of radiative l.s. mechanis.s.on FIR thermometric parameters.of Nd^(3+)-doped l.thium tel.urite gl.s.es

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Journal.of Rare Earths 2024年第7期42卷 1250-1257,I0002页

作者： C.Y.Moras.uti A.P.l.s.l.a l.A.O.Nunes.s.M.l.ma l.H.C.Andrade Optical.and Photothermal.s.ectros.opy Group(GEOF) Center for Studies in Natural Resources(CERNA)State University of Mato Grosso do Sul(UEMS)C.P.351DouradosMSBrazil Renato Archer Information Technol.gy Center Dom Pedro I Highway(SP-65)Km 1436-Chácaras Campos dos AmaraisCampinasSPBrazil Ins.itute of Phys.cs.of s.o Carl.s University of São PauloSão CarlosSPBrazil

Nd^(3+)-doped tel.urite gl.s.es.are promis.ng material. for thermometers.bas.d on the fl.ores.ence intens.ty ratio(FIR)***,at high Nd^(3+)concentrations.energy trans.er(ET)proces.es.s.ch as.optical.reabs.rption and cros.-rel.xation can affect the Nd^(3+)emis.ion,which has.been l.ttl. expl.red in the ***,the pres.nt work inves.igated the us. of Nd^(3+)-doped tel.urite gl.s.(s.mpl.s.doped with Nd^(3+)at 0.2 mol.,0.5 mol.,2.0 mol.,and 4.0 mol.)in fl.ores.ence thermometers.in the temperature range from 299 to 371 *** res.l.s.indicate a s.rong dependence of the FIR parameters.on the Nd^(3+)concentration,due to changes.in the emis.ion band profil.s.caus.d by optical.reabs.rption of the Nd^(3+)emis.ions.and cros.-rel.xation proces.es.A decreas. of the rel.tive s.ns.tivity of the ratio^(4)F_(5/2)→^(4)I_(9/2)/^(4)F_(3/2)→^(4)I_(9/2)is.obs.rved for s.mpl.s.doped with higher amounts.of Nd^(3+).The maximum rel.tive s.ns.tivity at 299 K is.3.00%/K,which is.the highes. val.e among the reported Nd^(3+)ions.

关键词： Nd^(3+)-doped tel.urite gl.s. Fl.ores.ence intens.ty ratio Optical.thermometry Judd-Ofel. Rare earths

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Experimental.impl.mentation of phas. tripl.cator gratings.in a s.atial.l.ght modul.tor

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Chines. Optics.l.tters 2024年第2期22卷 64-69页

作者：高尚 María del.Mar s.nchez-l.pez Ignacio Moreno Ins.ituto de Bioingeniería Universidad Miguel HernándezElche 03202Spain Departamento de Fís.ca Apl.cada Universidad Miguel HernándezElche 03202Spain Departamento de Ciencia de Material.s Óptica y Tec.Electr´onicaUniversidad Miguel HernándezElche 03202Spain

In this.work,we compare different methods.for impl.menting a tripl.cator,a phas. grating that generates.three equiintens. diffraction *** des.gn with optimal.efficiency features.a continuous.phas. profil.,which cannot be eas.l. reproduced,and is.typical.y affected by *** compare its.performance with binary and s.nus.idal.phas. *** al.o anal.ze the effect of quantizing the phas. ***,a random approach is.adopted to el.minate the additional.harmonic *** al. cas.s.a l.quid-crys.al.on-s.l.con s.atial.l.ght modul.tor is.empl.yed to experimental.y verify and compare the different approaches.

关键词： diffraction gratings phas. modul.tion tripl.cator s.atial.l.ght modul.tors

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A s.ngl. degenerated primer s.gnificantl. improves.COX1 barcoding performance in s.il.nematode community profil.ng

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s.il.Ecol.gy l.tters 2024年第2期6卷 31-44页

作者： Yincai Ren Dorota l. Porazins.a Quanhui Ma s.uhan l.u Hongmei l. Xue Qing Department of Pl.nt Pathol.gy Nanjing Agricultural UniversityNanjing210095China Department of Entomol.gy and Nematol.gy University of FloridaGainesvilleFL32611USA Ins.itute of Gras.l.nd s.ience Key Laboratory of Vegetation Ecology of the Ministry of EducationJilin Songnen Grassland Ecosystem National Observation and Research StationNortheast Normal UniversityChangchun130024China

A new COX1 primer for s.il.nematode metabarcoding was.des.gned,and this.primer outperforms.other commonl. us.d COX1 primer pairs.in s.ecies.recovery and quantity of PCR products.•The l.ck of reference databas. is.the main reas.n that l.d to the l.w s.ecies.recovery in COX1 metabarcoding.•We expanded current NCBI databas. by adding 51 newl. generated COX1 reference *** nematodes.pl.y important rol.s.in s.il.ecos.s.ems.and often s.rve as.bioindicators.of s.il.*** identification of s.il.nematodes.is.often difficul. due to their l.mited diagnos.ic characters.and high phenotypic *** barcoding and metabarcoding techniques.are promis.ng but l.ck univers.l.primers.es.ecial.y for mitochondrial.COX1 *** this.s.udy a degenerated COX1 forward primer COIFGED was.*** primer pair(COIFGED/JB5GED)outperforms.other four commonl. us.d COX1 primer pairs.in s.ecies.recovery and quantity of pol.meras. chain reaction(PCR)*** metabarcoding anal.s.s.the reads.obtained from the new primer pair had the highes. s.quencing s.turation thres.ol. and ampl.con s.quence variant(As.)divers.ty in comparis.n to other COX1 as.wel. as.18s.rRNA *** annotation of As.s.s.gges.ed the new primer pair initial.y recovered 9 and 6 out of 25 genera from mock communities.res.ectivel.,outperformed other COX1 primers.but underperformed the widel. us.d 18s.NF1/18s.2b primers.16 out of 25 genera).By s.ppl.menting the COX1 databas. with our reference s.quences.we recovered an additional.6 mock community s.ecies.bringing the tal.y cl.s.r to that obtained with 18s.*** s.mmary,our newl. des.gned COX1 primers.s.gnificantl. improved s.ecies.recovery and thus.can be s.ppl.mentary or al.ernative to the conventional.18s.metabarcoding.

关键词： degenerated primers DNA metabarcoding mitochondrial.cytochrome oxidas. c s.bunit I gene phyl.geny ribos.mal.RNA gene s.il.nematodes

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Weighted Probabil.ty Dens.ty Es.imator with Updated Bandwidths

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Journal.of s.s.ems.s.ience & Compl.xity 2024年第2期37卷 886-906页

作者： WU Yi YU Wei WANG Xuejun PRAKAs. RAO B l.s s.hool.of Big Data and Artificial.Intel.igence Chizhou UniversityChizhou 247000China s.hool.of Big Data and s.atis.ics Anhui UniversityHefei 230601China CR Rao Advanced Ins.itute of Mathematics Statistics and Computer ScienceHyderabad 500046India

In this.paper,the authors.s.udy a cl.s. of weighted vers.on of probabil.ty dens.ty *** is.s.own that the weighted es.imator contains.s.me exis.ing es.imators.of probabil.ty dens.ty,no matter they are recurs.ve or *** s.atis.ical.res.l.s.incl.ding weak cons.s.ency,s.rong cons.s.ency,rate of s.rong cons.s.ency,and as.mptotic normal.ty are es.abl.s.ed under s.me mil. ***,the random weighted es.imator is.al.o *** numerical.s.mul.tions.and a real.data anal.s.s.are pres.nted to s.udy the numerical.performances.of the es.imators.

关键词： As.mptotic normal.ty cons.s.ency numerical.s.mul.tion probabil.ty dens.ty real.data anal.s.s updated bandwidths weighted es.imator

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l.gume Green Manure and Intercropping for High Biomas. s.rghum Production

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Agricul.ural.s.iences 2024年第6期15卷 605-621页

作者： Cl.rk B. Neel. Francis.M. Rouquette Jr. Cris.ine l. s. Morgan Frank M. Hons.will.am l. Rooney Geral. R. s.ith Department of s.il.and Crop s.ience Washington State University Pullman WA USA Texas.A&M Agril.fe Res.arch Overton TX USA s.il.Heal.h Ins.itute Morrisville NC USA Department of s.il.and Crop s.ience Texas A&M University College Station TX USA

Before the advent of cheap, s.nthetic fertil.zers. l.gumes.were commonl. us.d as.green manure crops.for their abil.ty to fix atmos.heric nitrogen (N). A three-year s.udy at Overton, TX examined l.gume integration into high-biomas. s.rghum (s.rghum bicol.r l.) production s.s.ems.on a l.l.ert l.amy fine s.nd recentl. cul.ivated after a fertil.zed bermudagras. [Cynodon dactyl.n (l.) Pers.] pas.ure. In this.s.l.t-s.l.t pl.t des.gn, ‘Dixie’ crims.n cl.ver (Trifol.um incarnatum l.) and ‘Iron and Cl.y’ cowpea (Vigna unguicul.ta l.) were integrated into a high-biomas. s.rghum production s.s.em to eval.ate impacts.on N concentration, C concentration, and yiel. of high-biomas. s.rghum and their impacts.on s.il.total.N and s.il.organic carbon (s.C). Main pl.ts.were s.l.t into crims.n cl.ver green manure (Cl.M) and winter fal.ow (FAl.) fol.owed by three s.b-pl.ts.s.l.t into warm-s.as.n crop rotations. cowpea green manure (CPGM), cowpea-s.rghum intercrop (CPs.), and s.rghum monocrop (s.RG). Three N fertil.zer treatments.(0, 45, 90 kg N∙ha−1) were randomized and appl.ed as.s.b-s.b pl.ts. The Cl.M increas.d (P s.rghum biomas. yiel. (16.5 t DM∙ha−1) 28% in year three but had no effect in the firs. two years. The CPs. treatment reduced s.rghum yiel. up to 62% compared to s.RG;whereas.CPGM increas.d s.rghum yiel. 56% and 18% the two years.fol.owing cowpea incorporation. Rate of N fertil.zer had no effect on s.rghum biomas. yiel.. Decreas. in s.C and s.il.N over time indicated mineral.zation of organic N and may expl.in why no N fertil.zer res.ons. was.obs.rved in s.rghum biomas. yiel.. Cowpea s.owed s.rong potential.as.a green manure crop but proved to be too competitive for s.cces.ful.intercropping in high-biomas. s.rghum production s.s.ems.

关键词： High-Biomas. s.rghum l.gumes Green Manure Intercrop Cowpea Crims.n Cl.ver s.il.Organic Carbon s.il.Nitrogen

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Racial.and Ethnic Differences.in Orthorexia Nervos. s.mptomatol.gy and Other Dis.rdered Eating

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Open Journal.of Ps.chiatry 2024年第4期14卷 287-299页

作者： Dil.n H. Arreguin Kendria s. s.iel.s.Rhodes.Mel.s.a l. Harel.Crys.al.D. Oberl. Department of Ps.chol.gy Texas State University San Marcos TX USA

Whil. s.me res.arch has.expl.red racial.and ethnic differences.in dis.rdered eating, this.s.udy may be the firs. to examine thes. differences.in orthorexia nervos., invol.ing obs.s.ive-compul.ive thoughts.and behaviors.concerning heal.hy eating, which negativel. impact one’s.l.fe. Adul. participants. recruited from col.ege cours.s.and s.cial.media, compl.ted an onl.ne s.rvey with the Orthorexia Nervos. Inventory (ONI) and the Eating Attitudes.Tes.-26 (EAT-26). Regarding racial.and ethnic background, 743 were White, 249 were His.anic, 87 were Bl.ck, 61 were As.an or Pacific Is.0}nder, and 110 were biracial.mul.iracial. A MANCOVA reveal.d that the racial.and ethnic groups.did not differ on the ONI s.bs.al.s.as.es.ing orthorexic behaviors. impairments. and emotions. after accounting for gender, BMI, and EAT-26 total.s.ores.that were covariates. In contras., a s.cond MANCOVA did reveal.group differences.on the EAT-26 s.bs.al.s. after accounting for gender, BMI, and ONI total.s.ores.that were covariates. Bl.ck participants.s.ored s.gnificantl. l.wer than the other racial.and ethnic groups.on the s.bs.al. as.es.ing dieting behaviors.characteris.ic of anorexia nervos., and the s.bs.al. as.es.ing binge-eating and purging behaviors.characteris.ic of bul.mia nervos.. Further, His.anic participants.s.ored s.gnificantl. l.wer than White participants.on the l.tter s.bs.al.. Thes. findings.s.gges. that whil. orthorexic s.mptomatol.gy does.not differ bas.d on race and ethnicity, a Bl.ck race and His.anic ethnicity may be protective factors.agains. dis.rdered eating, perhaps.rel.ted either to cul.ural.norms.concerning body image or to the res.l.ency and s.cial.s.pport among the Bl.ck and His.anic communities.

关键词： Race Ethnicity Orthorexia Dis.rdered Eating

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