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High Sensitivity of Shotgun Metagenomic Sequencing in Colon Tissue Biopsy by Host DNA Depletion

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Genomics, Proteomics & Bioinformatics 2023年第6期21卷 1195-1205页

作者： Wing yin Cheng Wei-Xin Liu yanqiang Ding Guoping Wang yu Shi Eagle S.H.Chu Sunny Wong Joseph J.y.Sung Jun yu State Key Laboratory of Digestive Disease Institute of Digestive Disease and The Department of Medicine and TherapeuticsLi Ka Shing Institute of Health SciencesShenzhen Research InstituteThe Chinese University of Hong KongHong Kong Special Administrative Region 999077China Lee Kong Chian School of Medicine Nanyang Technology UniversitySingapore 639798Singapore

The high host genetic background of tissue biopsies hinders the application of shotgun metagenomic sequencing in characterizing the tissue *** proposed an optimized method that removed host DNA from colon biopsies and examined the effect on metagenomic *** or mouse colon biopsies were divided into two groups,with one group undergoing host DNA depletion and the other serving as the *** DNA was removed through differential lysis of mammalian and bacterial cells before *** impact of host DNA depletion on microbiota was compared based on phylogenetic diversity analyses and regression *** host DNA enhanced bacterial sequencing depth and improved species discovery,increasing bacterial reads by 2.46±0.20 folds while reducing host reads by 6.80%±1.06%.Moreover,2.40 times more of bacterial species were detected after host DNA *** was confirmed from mouse colon tissues,increasing bacterial reads by 5.46±0.42 folds while decreasing host reads by 10.2%±0.83%.Similarly,significantly more bacterial species were detected in the mouse colon tissue upon host DNA depletion(Py of shotgun metagenomic sequencing in bacteria detection in the biopsy,which may yield a more accurate taxonomic profile of the tissue microbiota and identify bacteria that are important for disease initiation or progression.

关键词： Gut microbiota Colon tissue Host DNAdepletion Shotgun metagenomic sequencing

Efficacy of Acid-treated Sawdust in Decolourization of Tanning Wastewater

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Journal of Environmental & Earth Sciences 2021年第2期3卷 55-60页

作者： M.Alhassan M.Suleiman A.A.Isah A.G.Abdulrashid y.Nasiru A.Bello Department of Chemistry Sokoto State UniversitySokotoNigeria Department of Chemistry Usmanu Danfodiyo UniversitySokotoNigeria

Colour removal from dye-bearing effluent is a serious challenge due to the difficulty in treating such wastewater by conventional treatment *** present investigation explores the decolourization of contaminated wastewater using acid-activated sawdust as an *** physicochemical properties of wastewater samples labelled A,B,and C viz average temperature;pH;electrical conductivity;and total dissolved solids were determined using standard methods to be 302.63;6.1;284.47μS/cm;35116.66 mg/L *** removal efficiency of the adsorbent was studied under variable conditions(contact time,rate of agitation,loading).Experimental results demonstrated that the sawdust adsorbent has a significant capacity for colour removal from tannery *** was significant variation in the absorbance of the treated *** dose,stirring rate,and contact time were found to be directly proportional to colour removal while pH variation of the samples show that the effluents became less alkaline(slightly acidic)after decolourization.

关键词： Wastewater Acid-modification Sawdust Physicochemical properties

Investigation of Multiphase Flows near Micro-textured Walls using Lattice Boltzmann Method

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Investigation of Multiphase Flows near Micro-textured Walls ...

THE REINFORCING MECHANISM OF TiB2 IN TiB2/A357 COMPOSITE

The 10th EPMESC

作者： y. T. Chew Department of Mechanical Engineering National University of Singapore 117576 Singapore

During the last decade, interest in multiphase flows near textured surface has revived due to its importance in microfluidics and the study of the self-cleaning property of some biological surfaces (o

关键词： lattice Boltzmann method micro-textured wall multiphase flows lotus effect

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Acta Metallurgica Sinica(English Letters) 2003年第5期16卷 375-378页

作者： B.yu y.J.Zhang Z.X.Zhao~ F.X.Zhao: Q.K.Cai C.S.Liu ShenyangResearchInstituteofFoundry TheChineseAcademyofMachinerySciencesShenyang110022China EastChinaUniversityofScienceandTechnology Shanghai200237China SchoolofMaterials&Metallurgy NortheasternUniversityShenyang110004China

Mechanical properties were tested for in situ TiB2/A357 composite fabricated by LSM (mixed salts reaction) method. Micro structures of as cast and plastic deformed TiB2/A357 were investigated. The results show that there is a low misfit between (200) Al and (101)TiB2 with [011]//Al [101]TiB2. There is a change from fully dendritic structure of the α-Al of A357 to a rosette-type structure of TiB2/A357. Significant increases in proof stress and young's modulus can be obtained at low TiB2 additions. There exist dislocation loops around neighboring TiB2 particles with about 0.1μm in diameter and dislocation multiplication near TiB2 particles.

关键词： TiB2/Al in situ composite reinforcing mechanism

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Type 2 diabetes mellitus and CA 19-9 levels

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World Journal of Gastroenterology 2007年第40期13卷 5357-5359页

作者： Oya Uygur-Bayramicli Resat Dabak Ekrem Orbay Can Dolapclo■lu Mehmet Sargin Gamze Kilico■lu yüksel Güleryüzlü Alpaslan Mayada■li Department of Gastroenterology Kartal State HospitalIstanbul 81110Turkey Department of Family Medicine Kartal State HospitalIstanbul 81110Turkey Department of Gastroenterology Kartal-State HospitalIstanbul 81110Turkey Department of Radiology Haydarpasa Numune State HospitalIstanbul 81110Turkey Department of Internal Medicine Kartal State HospitalIstanbul 81110Turkey Department of Radiotherapy Kartal State HospitalIstanbul 81110Turkey

AIM： To prospectively investigate serum CA 19-9 levels in type 2 diabetic patients in comparison with age and gender-matched control subjects.METHODS： We recorded duration of diabetes and examined fasting glucose levels, HbAlc levels and serum CA 19-9 levels in 76 type 2 diabetic patients and 76 controls. Abdominal CT was performed in order to eliminate abdominal malignancy in the diabetic and control groups.RESULTS： The average CA 19-9 level was 46.0 ± 22.4 U/mL for diabetic patients whereas it was 9.97± 7.1 U/mL for the control group （P 〈 0.001 ）. Regression analysis showed a positive correlation between diabetes and CA 19-9 independent from age, gender, glucose level and HbAlc level （t = 8.8, P 〈 001 ）. Two of the diabetic patients were excluded from the study because of abdominal malignancy shown by CT at the initial evaluation. For all patients, abdominal CT showed no pancreatic abnormalities. CONCLUSION： CA 19-9 is a tumor-associated antigen, which is elevated in pancreatic, upper gastrointestinal tract, ovarian hepatocellular, and colorectal cancers, as well as in inflammatory conditions of the hepatobiliary system, biliary obstruction and in thyroid diseases. Diabetes has been claimed to be a risk factor for pancreatic cancer, which is increasing its incidence and has one of the lowest survival rates of all cancers. CA 19-9 is used in the diagnosis of pancreatic cancer but is also a marker of pancreatic tissue damage that might be caused by diabetes. We propose that a higher cutoff value of CA 19-9 should be used in diabetics to differentiate benign and malignant pancreatic disease, and subtle elevations of CA 19-9 in diabetics should be considered as the indication of exocrine pancreatic dysfunction.

关键词： CA 19-9 Diabetes mellitus Chronic pancreatitis

如何实现从cdmaOne到cdma2000的演进

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电信科学 1999年第8期15卷 46-47页

作者： Leung. y 北电网络公司

１前言第三代（３Ｇ）无线技术旨于提供接近有线质量的无线话音业务，支持普通多媒体和高速数据应用所需的高速率和容量，以及其它先进业务。随着第三代技术的细节逐渐成为人们关注的焦点，包括政府、运营商、生产商及各种标准化机构在... 详细信息

１前言第三代（３Ｇ）无线技术旨于提供接近有线质量的无线话音业务，支持普通多媒体和高速数据应用所需的高速率和容量，以及其它先进业务。随着第三代技术的细节逐渐成为人们关注的焦点，包括政府、运营商、生产商及各种标准化机构在内的全球电信行业正努力制定使３Ｇ的...

关键词： cdmaOne cdma2000 CDMA 3G 无线通信

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Study on Structure and Dielectric Properties of Ce and Ca Co-doped BaTiO3 Ceramics

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Study on Structure and Dielectric Properties of Ce and Ca Co...

Linear free energy relationships between stability of complex and basicity of ligand Ⅶ. The copper(Ⅱ)-heteronuclear aromatic base-aminoacid temary systems

TEIM2015第六届无机材料结构、性能及测试表征技术研讨会

作者： D.y.Lu D.D.Han Q.L.Liu y.D.Wang X.y.Sun 吉林化工学院材料科学与工程研究中心

Structure and dielectric properties of 5%Ce and Ca co-doped BaTiO ceramics were *** site occupations and valence states were studied using XRD,Raman spectroscopy,SEM,EPR and dielectric *** Ba/Ti ratio has dramatic effects on ceramic structure and *** single-phase ceramics with Ba/Ti=1 and 0.937 have tetragonal and cubic structure,showing diffuse phase transition and first-order phase transition behaviors,*** former exhibit the site occupations of mixed Ca/Ceat Ba sites and Ca/Ceat Ti *** ions have priority over Caions in entering Ti sites as Cewhen co-doping in BaTiO,which will impede Caions to be incorporated completely into Ti sites.

关键词： Study on Structure and Dielectric Properties of Ce and Ca Co-doped BaTiO3 Ceramics

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Chinese Journal of Chemistry 1986年第3期4卷 189-195页

作者： CHEN Rong-Ti(y. T. Chen) LIN Hua-Kuan Department of Chemistry, Nankai University, Tianjin Department of Chemistry Nankai University Tianjin

The formation constants of ternary mixed ligand complex compounds formed fromCu（Ⅱ）-2,2'-bipyridyl （bipy） with N-（para-substituted phenyl） glycines （pRPhG）, Cu（Ⅱ）-1, 10-phenan-throline （phen） with N-（meta-substituted phenyl） glycines （mRPhG）, Cu（Ⅱ）--bipy/phen with someα-aminoacids （αA） have been determined by pH method at 25℃ in the presence of 0.10 M *** was found that linear free energy relationships do exist between the stability of ternary complexcompounds and the base strengths of the ligands in all four ternary systems investigated. The stabi-lity of the ternary complex compounds was discussed in terms of ΔlogKMand log X values. It wasfound again that linear relationships exist between Δ log KM, log X and p K2in the Cu （Ⅱ）-phen-mRPhGternary system.

关键词： heteronuclear aromatic base-aminoacid temary systems Linear free energy relationships between stability of complex and basicity of ligand The copper free base

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免疫球蛋白制品:无传播病毒的危险吗?

国外医学（输血及血液学分册） 1989年第6期12卷 383-384页

作者： Uemura y 叶农

曾设想未加热处理的免疫球蛋白制品无传播病毒的危险,主要依据是某些病毒已分离至废弃物中,且制品本身具有中和病毒的作用。因此其被用于预防包括甲、乙型肝炎在内的病毒感染。在常规筛检HBsAg前,不断有使用免疫球蛋白制品引起乙型肝炎... 详细信息

曾设想未加热处理的免疫球蛋白制品无传播病毒的危险,主要依据是某些病毒已分离至废弃物中,且制品本身具有中和病毒的作用。因此其被用于预防包括甲、乙型肝炎在内的病毒感染。在常规筛检HBsAg前,不断有使用免疫球蛋白制品引起乙型肝炎(乙肝)和非甲非乙(NANB)型肝炎的报道。以后。

关键词：免疫球蛋白病毒

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