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Food Bioscience 2022年第5期49卷 397-405页

作者： Bruno Dutra da silva Patricia Campos Bernardes Patrícia Fontes Pinheiro Juliana Di Giorgio Giannotti Consuelo Domenici roberto Department of Food Engineering Center for Agrarian Sciences and EngineeringUniversidade Federal do Espírito SantoAlegreEspírito SantoBrazil Department of Chemistry and Physics Center for ExactNatural and Health SciencesUniversidade Federal do Espírito SantoAlegreEspírito SantoBrazil Department of Rural Engineering Center for ExactNatural and Health SciencesUniversidade Federal do Espírito SantoAlegreEspírito SantoBrazil

Plectranthus amboinicus essential oil(PAEO)has the potential to be used by the food industry as a natural antimicrobial *** study aimed to evaluate the antibacterial activity of PAEO in vitro against pathogenic bacteria contaminating food and its potential for action when added to intentionally contaminated beef *** composition was determined by GC-MS and GC-FID,and thymol(45.64%)was the main compound present in the *** minimum inhibitory(MIC)and minimum bactericidal(MBC)concentration of the PAEO was evaluated against five pathogenic bacteria(Staphylococcus aureus,Listeria monocytogenes,Escherichia coli,Pseudomonas aeruginosa and Salmonella Typhimurium).The MIC of PAEO was the concentration ranges between 0.25 and 2 mg mL^(-1),and the MBC ranged from 1 to 4 mg mL^(-1).The anti-adhesion and removal of adhered cell assays indicated significant reductions(Pday of storage for ***(1.52 log CFU.g^(-1))and ***(0.93 log CFU.g^(-1))for beef patties with the MIC of *** has the potential to be applied in meat ***,the application and interactions of the oil in the food matrix need to be better elucidated.

关键词： Thymol Natural antimicrobial Anti-adhesion activity Minimum inhibitory concentration Antimicrobial activity

The relevance of James Lovelock’s research and philosophy to environmental science and academia

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Frontiers of Environmental Science & Engineering 2023年第3期17卷 143-144页

作者： Jaime A.Teixeira da silva Panagiotis Tsigaris Independent Researcher Ikenobe 3011-2Kagawa-ken761-0799Japan Department of Economics Thompson Rivers University805 TRU WayKamloopsBritish ColumbiaV2C 0C8Canada

James ***,famed for his Gaia hypothesis,which views the Earth as a living integrated and interconnected self-regulating system whose equilibrium comes about from complex energy-based interactions and feedback loops,ultimately sustaining life,passed away at the end of July,2022 at the age of *** only are the adaptive mechanisms of Gaia central to the conversation of environmental homeostasis,they lie at the heart of climate change and global *** is also remembered as the co-inventor of the electron capture detector that eventually allowed for the sensitive detection of chlorofluorocarbons and ***,Lovelock’s free-spirited nature and research independence allow academia to rethink current research’s modus operandi.

关键词： Academic independence Climate change Electron capture detector Environmental equilibrium Global warming Homeostasis

Taxing Digital Economy:A Critical View around the GloBE(Pillar Two)

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Frontiers of Law in China-Selected Publications from Chinese Universities 2020年第2期15卷 111-141页

作者： Bruno da silva University of Amsterdam AmsterdamThe Netherlands Amsterdam Centre for Tax Law University of AmsterdamAmsterdam 1018WB

The Organisation for Economic Co-operation and Development(OECD)proposal for taxation of digital economy constitutes one of the most ambitious projects in the field of taxation and may lead to the most significant reform to international tax rules in the 20th *** on a two-pillar approach,Pillar Two of the proposal suggests the adoption of Global Anti-Base Erosion(GloBE)provisions that are aimed at introducing a worldwide minimum *** this article,a critical analysis is based that the GloBE proposal suggests that it represents a shift in the OECD *** compared to base erosion and profit shifting(BEPS),it jeopardizes the tax sovereignty of jurisdictions and it raises fundamental challenges of implementation,both in terms of amendments to domestic law and conflicts with tax treaties.

关键词： Organisation for Economic Co-operation and Development(OECD) base erosion and profit shifting(BEPS) digital economy,minimum tax Pillar Two Global Anti-Base Erosion(GloBE) Global Intangible Low-Taxed Income(GILTI) Base Erosion Anti-Abuse Tax(BEAT) tax treaties

Equity issues and the PeCUS index:an indirect analysis of community severance

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Geo-Spatial Information Science 2020年第4期23卷 293-304页

作者： daniela Vanessa Rodriguez Lara Antonio Nelson Rodrigues da silva Department of Transportation Engineering São Carlos School of EngineeringUniversity of São PauloSão PauloBrazil

Urban barriers are often a consequence of the unplanned expansion of transport infrastructures,housing growth nearby existing transport infrastructures,and the vehicular traffic *** process,also known as“community severance”,is a problem that affects the travel patterns of the population and causes negative impacts to adjacent communities,but it is not easy to *** an alternative to the costly technologies and restricted-access data sometimes used to analyze urban barriers,we propose an analytical approach that assesses community severance levels by classifying the quality of Pedestrian Crossings on Urban Streets(the PeCUS index).Additionally,the approach is used to identify possible inequities nearby the different classification groups regarding demographic *** used the chi-square(χ^(2))test of independence and the standardized Pearson residuals to indicate deficits and excesses of residents regarding the demographic characteristics studied surrounding the urban *** found the following evidences of associations:residents with low-income or those aged up to 19 tend to live close to the worst crossings,whereas residents with permanent mobility constraints or the elderly tend to live near the crossings with the best ***,the study shows that the assessment of community severance by the analysis of the PeCUS index is ***,it reveals that the distribution of residents surrounding the distinct classes of quality of crossings is equitable for vulnerable social groups,except for low-income or young residents.

关键词： Community severance transport barriers pedestrian crossings equity open data geographic information systems(GIS)

Hydrocarbon indication in Rio Bonito Formation sandstone:Implication for CO_(2)storage in São Paulo,Brazil

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Energy Geoscience 2024年第1期5卷 331-341页

作者： Richardson M.Abraham-A Haline V.Rocha Saulo B.de Oliveira Colombo C.G.Tassinarri orlando C.da silva Institute of Energy and Environment University of São PauloSão PauloBrazil Research Centre for Greenhouse Gas Innovation University of São PauloSão PauloBrazil

São Paulo State has witnessed CO_(2)storage-based investigations considering the availability of suitable geologic structures and proximity to primary CO_(2)source sinks related to bioenergy and carbon capture and storage(BECCS)*** current study presents the hydrocarbon viability evaluations and CO_(2)storage prospects,focusing on the sandstone units of the Rio Bonito *** objective is to apply petrophysical evaluations with geochemical inputs in predicting future hydrocarbon(gas)production to boost CO_(2)storage within the study *** study used data from eleven wells with associated wireline logs(gamma ray,resistivity,density,neutron,and sonic)to predict potential hydrocarbon accumulation and fluid mobility in the investigated *** samples(shale and carbonate)obtained from depths>200 m within the study location have shown bitumen *** geochemistry data of the Rio Bonito Formation shale beds suggest they are potential hydrocarbon source rocks and could have contributed to the gas accumulations within the sandstone *** drilled well data,e.g.,CB-1-SP and TI-1-SP,show hydrocarbon(gas)presence based on the typical resistivity and the combined neutron-density responses at depths up to 3400 m,indicating the possibility of other hydrocarbon members apart from the heavy oil(bitumen)observed from the near-surface rocks *** the three-dimensional(3-D)model,the free fluid indicator(FFI)is more significant towards the southwest and southeast of the area with deeper depths of occurrence,indicating portions with reasonable hydrocarbon recovery rates and good prospects for CO_(2)injection,circulation and permanent ***,future studies based on contemporary datasets are required to establish the hydrocarbon viability further,foster gas production events,and enhance CO_(2)storage possibilities within the region.

关键词： ParanáBasin Hydrocarbon indication Sandstone reservoirs Rio Bonito Formation CO_(2)storage Hydrocarbon recovery factor Fluid injection rate

Exploring the role of mononuclear phagocytes in the epididymis

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Asian Journal of Andrology 2015年第4期17卷 591-596页

作者： Nicolas da silva Tegan B Smith Massachusetts General Hospital and Harvard Medical School Division of Nephrology Center for Systems Biology Boston Massachusetts USA.

The onslaught of foreign antigens carried by spermatozoa into the epididymis, an organ that has not demonstrated immune privilege, a decade or more after the establishment of central immune tolerance presents a unique biological challenge. Historically, the physical confinement of spermatozoa to the epididymal tubule enforced by a tightly interwoven wall of epithelial cells was considered sufficient enough to prevent cross talk between gametes and the immune system and, ultimately, autoimmune destruction. The discovery of an intricate arrangement of mononuclear phagocytes （MPs） comprising dendritic cells and macrophages in the murine epididymis suggests that we may have underestimated the existence of a sophisticated mucosal immune system in the posttesticular environment. This review consolidates our current knowledge of the physiology of MPs in the steady state epididymis and speculates on possible interactions between auto-antigenic spermatozoa, pathogens and the immune system by drawing on what is known about the immune system in the intestinal mucosa. Ultimately, further investigation will provide valuable information regarding the origins of pathologies arising as a result of autoimmune or inflammatory responses in the epididymis, including epididymitis and infertility.

关键词： antigen-presenting cells autoimmunity dendritic cells epididymis macrophages peripheral tolerance sperm maturation spermatozoa

Miscibility of Poly(hydroxybutyrate)/Poly(vinyl alcohol)Melt Blends Plasticized With Glycerol

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Journal of Renewable Materials 2019年第4期7卷 325-333页

作者： Deliane da silva Cabral Antonio JoséFelix Carvalho Department of Materials Engineering São Carlos School of EngineeringUniversity of São PauloAv.João Dagnone110013563-120São Carlos-SP-Brazil

Poly(hydroxybutyrate)/poly(vinyl alcohol)(PHB/PVA)blends plasticized with glycerol were prepared by melt blending of PHB and glycerol plasticized *** PHB/PVA-glycerol compositions were 90:10,75:25 and 50:50 w/w,being the concentration of glycerol in the PVA mixture of 10 wt%.The blends were characterized by infrared spectroscopy,dynamic-mechanical thermal analysis,and scanning electron microscope of the fragile fractured *** results showed one single phase blend,indicating miscibility corroborated by the presence of a single glass transition *** blending method proved to be an efficient way to tune PHB properties keeping its biodegradable nature since both PVA and glycerol are fully biodegradable materials.

关键词： Blend miscibility melt processing poly(hydroxybutyrate) poly(vinyl alcohol)

Radiographic and tomographic description of marlin sucker Remora osteochir, Pisces: Echeneidae—preliminary data of one specimen

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Marine Life Science & Technology 2020年第3期2卷 246-251页

作者： AndréLuiz Veiga Conrado Renata Stecca lunes Carlos Eduardo Malavasi Bruno Aline Tiemi Shiraishi Rocha Joséroberto Machado Cunha da silva Laboratory of Evolutive Histophysiology Department of Cell and Developmental BiologyInstitute of Biomedical SciencesUniversity of Sao PauloAvenida ProfLineu Prestes1524Cidade UniversitariaSao Paulo 05508-000Brazil Laboratory of Immunological Techniques Applied to Morphophysiology School of Veterinary Medicine and Animal ScienceDepartment of SurgeryUniversity of Sao PauloAvenida Prof.Di.Orlando Marques de Paiva87.Cidade UniversitariaSao Paulo 05508-270Brazil

Remoras are commensal fish of various marine species,such as sharks,swordfishes,turtles,dolphins,manta rays and *** specimen of marlin sucker Remora osteochir was evaluated using computed tomography for skeletal digital reconstruction,digital radiology for general evaluation and bone counting,and the double contrast technique to distinguish coelomic *** radiographic images,it was possible to observe otoliths in the center of the neurocranium,to count 27 vertebrae,nine pairs of ribs,and to detect the presence of the hipural and epural bones near of the caudal *** double contrast images,it was possible to visualize the swim bladder,gills,heart,liver,stomach and *** the tomographic images and reconstructions,it was possible to identify the intercalar bones of the cephalic disc;the spine with vertebral bodies composed of neural arches and ventral ribs;and the pectoral girdle formed by posttemporal,cleithrum and scapulocoracoid *** was concluded that digital radiology and computed tomography were able to describe anatomical structures of marlin sucker *** without the need for dissection.

关键词： Teleost fish Double contrast Computed tomography Digital radiology Skeleton

Landesman–Lazer Type Conditions and Multiplicity Results for Nonlinear Elliptic Problems with Neumann Boundary Values

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Acta Mathematica Sinica,English Series 2014年第2期30卷 229-250页

作者： Edcarlos Domingos da silva Francisco Odair DE PAIVA Universidade Federal de Gois Departamento de Matemtica Universidade Federal de So Carlos Departamento de Matemtica

We establish the existence and multiplicity of solutions for Steklov problems under non- resonance or resonance conditions using variational methods. In our main theorems, we consider a weighted eigenvalue problem of ... 详细信息

关键词： Neumann-Steklov eigenvalue problems resonance Landesman-Lazer conditions

EVOLUTION OF THE BENEFICIAL OWNERSHIP CONCEPT： MORE THAN HALF OF CENTURY OF UNCERTAINTY AND WHAT HISTORY CAN TELL US

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Frontiers of Law in China-Selected Publications from Chinese Universities 2017年第4期12卷 501-523页

作者： Bruno da silva Ph.D. in International Tax Law Faculty of Law University of Amsterdam Amsterdam

The beneficial ownership concept has constituted for more than half a century one of the most fundamental and debated issues in the application of tax treaties. This article goes back to routes of this term explaining the reason of its original inclusion in the OECD Model Tax Convention and why ultimately such inclusion may have not been necessary. Then it analyses the historical developments of beneficial ownership in the OECD Model Tax Convention. For that purpose it considers different interpretations adopted by jurisdictions （particularly in China） and local courts delving into some of the landmark cases on the subject. Finally it provides a detailed analysis of the current meaning of beneficial ownership considering the most recent developments in the Commentary to the OECD Model Tax Convention.

关键词： beneficial ownership OECD Model Tax Convention Commentary,interpretation case-law China