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Photobiomodulation:a novel approach to promote trans-differentiation of adipose-derived stem cells into neuronal-like cells

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Neural Regeneration Research 2025年第2期20卷 598-608页

作者： daniella da silva Madeleen Jansen van Rensburg Anine Crous Heidi Abrahamse Laser Research Centre Faculty of Health SciencesUniversity of JohannesburgDoornfonteinJohannesburgSouth Africa

Photobiomodulation,originally used red and near-infrared lasers,can alter cellular *** has been demonstrated that the visible spectrum at 451-540 nm does not necessarily increase cell proliferation,near-infrared light promotes adipose stem cell proliferation and affects adipose stem cell migration,which is necessary for the cells homing to the site of *** this in vitro study,we explored the potential of adipose-derived stem cells to differentiate into neurons for future translational regenerative treatments in neurodegenerative disorders and brain *** investigated the effects of various biological and chemical inducers on trans-differentiation and evaluated the impact of photobiomodulation using 825 nm near-infrared and 525 nm green laser light at 5 J/*** adipose-derived stem cells can be used in autologous grafting and photobiomodulation has been shown to have biostimulatory *** findings reveal that adipose-derived stem cells can indeed trans-differentiate into neuronal cells when exposed to inducers,with pre-induced cells exhibiting higher rates of proliferation and trans-differentiation compared with the control ***,green laser light stimulation led to notable morphological changes indicative of enhanced trans-differentiation,while near-infrared photobiomodulation notably increased the expression of neuronal *** biochemical analysis and enzyme-linked immunosorbent assays,we observed marked improvements in viability,proliferation,membrane permeability,and mitochondrial membrane potential,as well as increased protein levels of neuron-specific enolase and ciliary neurotrophic ***,our results demonstrate the efficacy of photobiomodulation in enhancing the trans-differentiation ability of adipose-derived stem cells,offering promising prospects for their use in regenerative medicine for neurodegenerative disorders and brain injuries.

关键词： differentiation inducers green photobiomodulation immortalized adipose-derived stem cell near-infrared photobiomodulation neurodegenerative disease neurogenesis photobiomodulation trans-differentiation

Conflicting Discourses on Wildfire Risk and the Role of Local Media in the Amazonian and Temperate Forests

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International Journal of Disaster Risk Science 2019年第4期10卷 529-543页

作者： Nathalia Thais Cosmo da silva Urbano FraPaleo Jose Ambrosio Ferreira neto Departamento de Economia Rural Universidade Federal de VicosaCampus UniversitarioEdificio Edson Potsch MagalhaesVicosaMG 36570-900Brazil School of Social Sciences and Humanities University of ExtremaduraCampus of Caceres City10071 CaceresSpain

This article examines how risk is communicated by different actors,particularly local print newspapers and actors at the community level,in two different geographical contexts that are severely affected by wildfires—the Brazilian Amazon and Atlantic *** analyzed how wildfire risk is framed in local print media and local actor discourse to elucidate how wildfire risk is interpreted and aimed to identify the main priorities of these risk governance *** main findings reveal that the presentation of wildfire as a spectacle is a serious obstacle to the promotion of coherent risk governance and social learning,which involves recognizing wildfire risk as a social,political,economic,and environmental *** risk governance should communicate the multifaceted nature of risk and stimulate dialogue and negotiation among all actors to build consensus regarding land use and the creation of risk.

关键词： Discourse analysis Framing Galicia Local media Risk communication Rondonia Wildfire risk

CFD predictions for hazardous area classification

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Chinese Journal of Chemical Engineering 2019年第1期27卷 21-31页

作者： Andrey Oliveira de Souza Aurélio Moreira Luiz Ant?nio Tavernard Pereira neto Ant?nio Carlos Brandao de Araujo Heleno Bispo da silva Sidinei Kebler da silva Jose Jailson Nicacio Alves Federal Institute of Education Science and Technology of Paraiba Petrobras-Petróleo Brasileiro S.A. Federal University of Campina Grande

This study aimed to describe a Computational Fluid Dynamics(CFD) procedure using the ANSYS CFX software 16.1 and Design of Experiments for the determination of volume and extension of explosive atmospheres due to fugitive emissions of flammable *** multidimensional statistical sampling technique Latin Hypercube was used, which defined 100 simulations of random methane gas leak *** CFD model proved to be robust in predicting the extension and volume of the explosive atmosphere for orifice diameters from 0.1 to2.5 mm, pressure from 0.1 MPa to 12 MPa and temperatures from 0 ℃ to 400 ℃.It was found that the calculation domain must be parameterized 8 m in length for each millimeter of the diameter of the source of release to ensure the *** order not to lose precision for very small diameters, the mesh was parameterized with 50 elements along the orifice *** was proved that gravity does not influence the extension and volume of the explosive atmosphere at sonic *** deviation from the ideal gas behavior in the reservoir,achieved by applying the Soave–Redlich–Kwong equation of state, also has not significantly influenced the extension and volume of the explosive *** results showed that the size of the explosive atmosphere varies directly with the diameter of the source emission and reservoir pressure, and inversely with the temperature of the *** diameter of the source is the parameter that has the major effect on the extension of the explosive atmosphere, followed by the pressure and lastly the temperature of the reservoir.

关键词： Computational Fluid Dynamics Computer simulation Safety Fugitive emissions Classified area Flammable gas cloud

Spatial-temporal distribution of fatal yellowing in different oil palm genetic materials in eastern amazon

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Information Processing in Agriculture 2022年第3期9卷 365-377页

作者： Bruno Borella Anhe Artur Vinicius Ferreira dos Santos Thiago Alan Ferreira da silva Lana Leticia Barbosa de Carvalho Paulo roberto silva Farias Universidade Federal Rural da Amazonia Geoestatistics Department66.077-830 BelemBrazil Universidade Federal Rural da Amazonia Entomology Department66.077-830 BelemBrazil

Oil palm (Elaeis guineensis Jacq.) is one of the agricultural crops with the greatest potentialfor vegetable oil production in Brazil. However, a disease of unknown etiology popularlyknown as Fatal Yellowin (FY) has caused damage to Brazilian farmers particularly in theeastern region of the Amazon. So, the objective of this study was to evaluate the spatialdependence of FY on three oil palm genotypes, grown for many years in an organic produc-tion system in the Amazon region. The study area had 4104 ha, divided into 139 plots. Ineach plot, the monthly incidence of disease was monitored forming a database. The num-ber of diseased plants per year, number of accumulated diseased plants, number of dis-eased plants per hectare, growth rate of diseased plants and incidence of accumulateddisease were evaluated. The results indicated spatial distribution of the variables adjustedto the gaussian, spherical and exponential models, with predominance of the first *** increases the purpose that FY is caused by biotic factors. The highest range achievedin the study was 2929 m indicating the susceptibility of the tested genotypes. Some plotsclose to the river had the highest incidence of the disease on the study, probably associatedwith higher soil moisture.

关键词： Elaeis guineensis Geostatiscal analysis Kriging Palm oil Spatial dependence

Integrated Design Fabrication and Control of a Bioinspired Multimaterial Soft Robotic Hand

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Cyborg and Bionic Systems 2023年第1期4卷 156-165页

作者： Samuel Alves Mihail Babcinschi Afonso silva Diogo neto Diogo Fonseca Pedro neto University of Coimbra CEMMPREARISEDepartment of Mechanical Engineering3030-788 CoimbraPortugal

Machines that mimic humans have inspired scientists for *** soft robotic hands are a good example of such an endeavor,featuring intrinsic material compliance and continuous motion to deal with uncertainty and adapt to unstructured *** research led to impactful achievements in functional designs,modeling,fabrication,and control of soft ***,the full realization of life-like movements is still challenging to achieve,often based on trial-and-error considerations from design to fabrication,consuming time and *** this study,a soft robotic hand is proposed,composed of soft actuator cores and an exoskeleton,featuring a multimaterial design aided by finite element analysis(FEA)to define the hand geometry and promote finger’s *** actuators are fabricated using molding,and the exoskeleton is 3D-printed in a single *** ON-OFF controller keeps the set fingers’inner pressures related to specific bending angles,even in the presence of *** FEA numerical results were validated by experimental tests,as well as the ability of the hand to grasp objects with different shapes,weights,and *** integrated solution will make soft robotic hands more available to people,at a reduced cost,avoiding the time-consuming design-fabrication trial-and-error processes.

关键词： consuming skeleton realization

Small-scale self-driving cars:A systematic literature review

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Journal of Traffic and Transportation Engineering(English Edition) 2024年第2期11卷 271-292页

作者： Felipe Caleffi Lauren da silva Rodrigues Joice da silva Stamboroski Brenda Medeiros Pereira Autonomous Vehicles Laboratory Federal University of Santa MariaCachoeira do Sul 96503-205Brazil Mobility and Logistics Laboratory Federal University of Santa MariaCachoeira do Sul 96503-205Brazil

The autonomous vehicle(AV)technology has the potential to significantly improve safety and efficiency of the transportation and logistics ***-scale AV testing is limited by time,space,and cost,while simulation-based testing often lacks the necessary accuracy of AV and environmental *** recent years,several initiatives have emerged to test autonomous software and hardware on scaled *** systematic literature review provides an overview of the literature surrounding small-scale self-driving cars,summarizing the current autonomous platforms deployed and focusing on the software and hardware developments in this *** studies published in English-language journals or conference papers that present small-scale testing of self-driving cars were *** of Science,Scopus,Springer Link,Wiley,ACM Digital Library,and TRID databases were used for the literature *** systematic literature search found 38 eligible *** gaps in the reviewed papers were identified to provide guidance for future *** key takeaway emerging from this manuscript are:(i)there is a need to improve the models and neural network architectures used in autonomous driving systems,as most papers present only preliminary results;(ii)increasing datasets and sharing databases can help in developing more reliable control policies and reducing bias and variance in the training process;(iii)small-scaled vehicles to ensure safety is a major benefit,and incorporating data about unsafe driving behaviors and infrastructure problems can improve the accuracy of predictive models.

关键词： Self-driving car Autonomous vehicle Small-scale vehicle Systematic literature review

Physiological plasticity in eucalyptus clones in the vegetative stage contributes to drought tolerance

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Journal of Forestry Research 2023年第5期34卷 1549-1561页

作者： Vilma Marques Ferreira Jailma Ribeiro de Andrade Claudiana Moura dos Santos Sebastiao de Oliveira Maia Júnior Joao Correia de Araújo neto Andrea Francisca da silva Santos Vicente Mota da silva Lucas Teles Bezerra Laurício Endres Laboratório de Fisiologia Vegetal Campus de Engenharia e Ciências AgráriasUniversidade Federal de AlagoasRio LargoAL57072-900Brazil Laboratório de Ciências Ambientais e Biodiversidade Departamento de Zootecnia.Centro de Ciências AgráriasUniversidade Estadual do MaranhãoSão LuísMA65300-000Brazil Laboratório de Propagação de Plantas.Campus de Engenharia e Ciências Agrárias Universidade Federal de AlagoasRio LargoAL57072-900Brazil Laboratório de Lepidoptera Museu de ZoologiaUniversidade de São PauloIpirangaSão PauloSP04263-000Brazil

With the expansion of eucalyptus crops to areas with severe water limitations,physiological studies involving eucalyptus clones to identify those that are tolerant to water stress become *** objective of this study was to assess morphological and physiological responses by eucalyptus clones subjected to drought stress and *** experiment consisted of three eucalyptus clones:VC865,I224 and I144 and two water regimes:control and water stress followed by rehydration,with six *** water potential,gas exchange,maximum quantum efficiency of photo systemⅡand plant height and stem diameter were evaluated under drought stress and ***6 d of rehydration,the number of leaves,leaf area and dry mass of root,leaf,stem and their total were *** clones showed intense reduction of gas exchange during the drought stress period,and only VC865 and 1144 showed rapid recovery with 3 d of *** 1224 showed greater reduction in height,stem diameter,number of leaves,water potential at midday(Ψ_(w)_(Midday)),and maximum quantum efficiency of photosystemⅡ(F_(v)/F_(m)).Clones VC865and T144 showed lower reductions inΨ_(wMidday)and F_(v)/F_(m) under ***865 had lower reductions in leaf number,leaf area and higher leaf dry mass,while clone I144 had higher height and lower reduction in root dry mass *** these clones showed higher water use efficiency with 3d of *** different phenotypic plasticities gave the clones VC865 and 1144 efficient mechanisms of acclimatization to stress and more drought tolerance,enhancing their greater capacity for recovery after stress,which allowed lower dry mass *** 1224,however,was more susceptible to drought stress,undergoing greater physiological damage with only partial recovery during rehydration.

关键词： Drought Eucalyptus species Gas exchanges Post-stress Root dry mass

A Bidimensional Finite Element Study of Crack Propagation in Austempered Ductile Iron

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Computers, Materials & Continua 2023年第11期77卷 1411-1424页

作者： Gustavo von Zeska de França roberto Luís de Assumpção Marco Antonio Luersen Carlos Henrique da silva Department of Mechanical Engineering Federal University of Technology–ParanáCuritiba81280-340Brazil

Austempered ductile iron(ADI)is composed of an ausferritic matrix with graphite nodules and has a wide range of applications because of its high mechanical strength,fatigue resistance,and wear resistance compared to other cast *** amount and size of the nodules can be controlled by the chemical composition and austenitizing *** the nodules have lower stiffness than the matrix and can act as stress concentrators,they influence crack ***,the crack propagation mechanism in ADI is not yet fully *** this study,we describe a numerical investigation of crack propagation in ADIs subjected to cyclic *** numerical model used to calculate the stress intensity factors in the material under the given conditions is built with the aid of Abaqus commercial finite element *** crack propagation routine,which is based on the Paris law,is implemented in *** results of the simulation show that the presence of a nodule generates a shear load on the crack ***,even under uniaxial tensile loading,the presence of the nodule yields a non-zero stress intensity factor in mode II,resulting in a deviation in the crack propagation *** is the primary factor responsible for changing the crack propagation direction towards the *** the parameters,for example,increasing the nodule size or decreasing the distance between the nodule and crack tip,can intensify this *** simulations comparing two different ADIs with the same graphite fraction area,the crack in the material with more nodules reaches another nodule in a shorter propagation time(or shorter number of cycles).This suggests that the high fatigue resistance observed in ADIs may be correlated with the number of nodules intercepted by a crack and the additional energy required to nucleate new *** summary,these findings contribute to a better understanding of crack propagation in ADIs,provide insights into the relationship between the presence

关键词： Austempered ductile iron(ADI) fatigue crack propagation graphite nodule

Evolution, divergence, and convergence in the mandibles of opossums (Didelphidae, Didelphimorphia)

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Current Zoology 2024年第4期70卷 488-504页

作者： Franciscodas Chagas silva-neto Silvia E.Pavan Diego Astua Laboratorio de Mastozoologia Departamento de ZoologiaUniversidade Federal de Pernambuco.Av.Prof.Moraes Regos.n.Cidade Universitaria.50670-901RecifePEBrazil Department of Biological Sciences California State Polytechnic UniversityHumboldt1Harpst StreetArcataCA95521USA

Didelphid marsupials are considered a morphologically unspecialized group with a generalist diet that includes vertebrates,invertebrates,and plant *** cranium and scapula variation has alreadybeen examined within Didelphidae,variation inmandible shape,usually associated with diet or phylogeny in other mammalian groups,has not yet been properly assessed in the *** evaluated the variation in mandible shape and size of didelphids(2470 specimens belonging to 94 species)using 2D geometric *** classified the diet of the didel-phids into four broad categories to assess whether morphospace ordination relates to dietary *** also provided the most comprehensive phylogeny for the family(i23 out of the 126 living species)using 10 nuclear and mitochondrial *** then mapped mandible size and shape onto that phylogeny for 93 selected taxa and ancestral size and shapes were reconstructed by *** found phylogenetically structured variation in mandible morphology between didelphid groups,and our results indicate that they have a significant phylogenetic *** main axis of shape variation is poorly related to size,but the second is strongly allometric,indicating that allometry is not the main factor in shaping morphological diversity on their *** results indicate that the shape and size of the ancestral mandible of didelphids would be similar to that of the current species of the genus Marmosa.

关键词： mandible Didelphimorphia morphological evolution allometry phylogeny diet.