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Signal Transduction and Targeted Therapy 2024年第7期9卷 3112-3129页

作者： Yuzheng Zhou Zongpeng Chen Sijie Liu Sixu Liu Yujie Liao Ashuai Du Zijun Dong Yongxing Zhang Xuan Chen Siyi Tao Xin Wu Aroona Razzaq Gang Xu de-an Tan Shanni Li Youwen deng Jian Peng Shuyan Dai Xu deng Xianwen Zhang Taijiao Jiang Zheng Zhang Gong Cheng Jincun Zhao Zanxian Xia department of Cell Biology School of Life SciencesCentral South University410013 ChangshaChina Institute for Hepatology National Clinical Research Center for Infectious DiseaseShenzhen Third People’s HospitalThe Second Affiliated HospitalSchool of MedicineSouthern University of Science and Technology518112 ShenzhenChina department of Basic Medicine School of MedicineHunan Normal University410081 ChangshaChina department of spine surgery The Third Xiangya HospitalCentral South University410013 ChangshaChina School of Basic Medical Sciences Anhui Medical University230032 HefeiChina Hunan Key Laboratory of Neurorestoratology 921 Hospital of Joint Logistics Support Force People’s Liberation Army of China(The Second Affiliated Hospital of Hunan Normal University)410003 ChangshaHunanChina department of Geriatric Surgery Xiangya HospitalCentral South University410008 ChangshaChina Xiangya School of Pharmaceutical Sciences Central South University410013 ChangshaChina Institute of Infectious Diseases Shenzhen Bay Laboratory518132 ShenzhenChina Guangzhou Laboratory 510005 GuangzhouChina Tsinghua University-Peking University Joint Center for Life Sciences School of MedicineTsinghua University100084 BeijingChina State Key Laboratory of Respiratory Disease National Clinical Research Center for Respiratory DiseaseGuangzhou Institute of Respiratory HealthThe First Affiliated Hospital of Guangzhou Medical University510120 GuangzhouChina Hunan Key Laboratory of Animal Models for Human Diseases Hunan Key Laboratory of Medical Genetics&Center for Medical GeneticsSchool of Life SciencesCentral South University410008 ChangshaChina

The ORF9b protein,derived from the nucleocapsid’s open-reading frame in both SARS-CoV and SARS-CoV-2,serves as an accessory protein crucial for viral immune evasion by inhibiting the innate immune *** its significance,the precise regulatory mechanisms underlying its function remain *** the present study,we unveil that the ORF9b protein of SARS-CoV-2,including emerging mutant strains like delta and omicron,can undergo ubiquitination at the K67 site and subsequent degradation via the proteasome pathway,despite certain mutations present among these ***,our investigation further uncovers the pivotal role of the translocase of the outer mitochondrial membrane 70(TOM70)as a substrate receptor,bridging ORF9b with heat shock protein 90 alpha(HSP90α)and Cullin 5(CUL5)to form a *** this complex,CUL5 triggers the ubiquitination and degradation of ORF9b,acting as a host antiviral factor,while HSP90αfunctions to stabilize ***,treatment with HSP90 inhibitors such as GA or 17-AAG accelerates the degradation of ORF9b,leading to a pronounced inhibition of SARS-CoV-2 ***-cell sequencing data revealed an up-regulation of HSP90αin lung epithelial cells from COVID-19 patients,suggesting a potential mechanism by which SARS-CoV-2 may exploit HSP90αto evade the host *** study identifies the CUL5-TOM70-HSP90αcomplex as a critical regulator of ORF9b protein stability,shedding light on the intricate host–virus immune response dynamics and offering promising avenues for drug development against SARS-CoV-2 in clinical settings.

关键词： stability modulator offering

Diagnosis,treatment and prevention of severe acute respiratory syndrome coronavirus 2 infection in children:experts'consensus statement updatedforthe omicron variant

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World Journal of Pediatrics 2024年第3期20卷 272-286页

作者： Rong-Meng Jiang Zheng-de Xie Yi Jiang Xiao-Xia Lu Run-Ming Jin Yue-Jie Zheng Yun-Xiao Shang Bao-Ping Xu Zhi-Sheng Liu Gen Lu Ji-Kui deng Guang-Hua Liu Xiao-Chuan Wang Jian-She Wang Lu-Zhao Feng Wei Liu Yi Zheng Sai-Nan Shu Min Lu Wan-Jun Luo Miao Liu Yu-Xia Cui Le-Ping Ye A-Dong Shen Gang Liu Li-Wei Gao Li-Juan Xiong Yan Bai Li-Kai Lin Zhuang Wei Feng-Xia Xue Tian-You Wang Dong-Chi Zhao Jian-Bo Shao Daniel Kwok-keung Ng Gary Wing-kin Wong Zheng-Yan Zhao Xing-Wang Li Yong-Hong Yang Kun-Ling Shen Diagnosis and Treatment Center of Infectious Diseases Beijing Ditan HospitalCapital Medical UniversityBeijing 100015China Beijing Key Laboratory of Pediatric Respiratory Infection Diseases Key Laboratory of Major Diseases in ChildrenMinistry of EducationNational Clinical Research Center for Respiratory DiseasesResearch Unit of Critical Infection in ChildrenChinese Academy of Medical Sciences2019RU016Laboratory of Infection and VirologyBeijing Pediatric Research InstituteBeijing Children's HospitalCapital Medical UniversityNational Center for Children's HealthBeijing 100045China department of Pediatrics Renmin Hospital of Wuhan UniversityWuhan 430060China department of Respiratory Wuhan Children's HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430016China department of Pediatrics Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China department of Respiratory Shenzhen Children's HospitalShenzhen 518038China department of Pediatric Respiratory Shengjing Hospital Affiliated to China Medical UniversityShenyang 110004China department of Respiratory Beijing Children's HospitalCapital Medical UniversityNational Clinical Research Center for Respiratory DiseasesNational Center for Children's HealthBeijing 100045China department of Neurology Wuhan Children's HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430016China department of Respiratory Guangzhou Women and Children's Medical CenterGuangzhou 510623China department of Infectious Diseases Shenzhen Children's HospitalShenzhen 518038China department of Pediatrics Fujian Branch of Shanghai Children's Medical CenterFujian Children's HospitalFuzhou350005China department of Clinical Immunology and Allergy Children's Hospital of Fudan UniversityNational Center for Children's HealthShanghai 201102China department of Infectious Diseases Children's Hospital of Fudan UniversityNational Center for Children's Health.Shanghai

It has been more than 3 years since the novel coronavirus(SARS-CoV-2)pandemic raged *** coronavirus disease 2019(COVID-19)has greatly influenced human *** to data from the World Health Organization(WHO),there were ove... 详细信息

关键词： prevention infection statement

Genomic Perspectives on the Emerging SARS-CoV-2 omicron Variant

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Genomics, Proteomics & Bioinformatics 2022年第1期20卷 60-69页

作者： Wentai Ma Jing Yang Haoyi Fu Chao Su Caixia Yu Qihui Wang Ana Tereza Ribeiro de Vasconcelos Georgii A.Bazykin Yiming Bao Mingkun Li CAS Key Laboratory of Genomic and Precision Medicine Beijing Institute of GenomicsChinese Academy of Sciences and China National Center for BioinformationBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China CAS Key Laboratory of Pathogenic Microbiology and Immunology Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China National Genomics Data Center Beijing Institute of GenomicsChinese Academy of Sciences and China National Center for BioinformationBeijing 100101China Laboratorio de Bioinformatica Laboratorio Nacional de Computacao CientıficaPetropolis 25651-075Brazil Skolkovo Institute of Science and Technology Moscow 121205Russia Kharkevich Institute for Information Transmission Problems of the Russian Academy of Sciences Moscow 127051Russia Center for Excellence in Animal Evolution and Genetics Chinese Academy of SciencesKunming 650201China

A new variant of concern for SARS-CoV-2,omicron(B.1.1.529),was designated by the World Health Organization on November 26,*** study analyzed the viral genome sequencing data of 108 samples collected from patients infected with ***,we found that the enrichment efficiency of viral nucleic acids was reduced due to mutations in the region where the primers anneal ***,the omicron variant possesses an excessive number of mutations compared to other variants circulating at the same time(median:62 vs.45),especially in the Spike *** in the Spike gene confer alterations in 32 amino acid residues,more than those observed in other SARS-CoV-2 ***,a large number of nonsynonymous mutations occur in the codons for the amino acid residues located on the surface of the Spike protein,which could potentially affect the replication,infectivity,and antigenicity of ***,there are 53 mutations between the omicron variant and its closest sequences available in public *** of these mutations were rarely observed in public databases and had a low mutation *** addition,the linkage disequilibrium between these mutations was low,with a limited number of mutations concurrently observed in the same genome,suggesting that the omicron variant would be in a different evolutionary branch from the currently prevalent *** improve our ability to detect and track the source of new variants rapidly,it is imperative to further strengthen genomic surveillance and data sharing globally in a timely manner.

关键词： omicron Genomics Mutation Variant of concern SARS-CoV-2

Dicoumarol is an effective post-exposure prophylactic for SARS-CoV-2 omicron infection in human airway epithelium

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Signal Transduction and Targeted Therapy 2023年第7期8卷 3455-3465页

作者： Yang Peng Shi-ying Chen Zhao-ni Wang Zi-qing Zhou Jing Sun Gui-an Zhang Jia Li Lei Wang Jin-cun Zhao Xiao Xiao Tang de-Yun Wang Nan-shan Zhong State Key Laboratory of Respiratory Disease National Clinical Research Center for Respiratory DiseaseGuangzhou Institute of Respiratory HealthThe First Affiliated Hospital of Guangzhou Medical UniversityGuangzhou Medical UniversityGuangzhouGuangdongChina department of Otolaryngology Infectious Diseases Translational Research ProgramYong Loo Lin School of MedicineNational University of SingaporeSingaporeSingapore Guangzhou Laboratory GuangzhouChina

Repurposing existing drugs to inhibit SARS-CoV-2 infection in airway epithelial cells(AECs)is a quick way to find novel treatments for *** screening has found dicoumarol(DCM),a natural anticoagulant,to be a potential SARS-CoV-2 inhibitor,but its inhibitory effects and possible working mechanisms remain *** air-liquid interface culture of primary human AECs.

关键词： Omi infection epithelium

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新型冠状病毒关注变异株抗体反应研究

中国医学科学院学报 2023年第3期45卷 454-463页

作者：朱衣兴常德中国人民解放军总医院第八医学中心呼吸与危重症医学部北京100026 中国人民解放军总医院第七医学中心呼吸与危重症医学科北京100091

迄今为止,新型冠状病毒感染大流行已有3年,累计感染约7亿人,造成600多万人死亡,给人类社会造成了极大的影响。据全球共享流感数据倡议组织统计,SARS-CoV-2的变异序列超过1200万种,其中影响力最大的是5种关注变异株:Alpha、Beta、Gamma、... 详细信息

迄今为止,新型冠状病毒感染大流行已有3年,累计感染约7亿人,造成600多万人死亡,给人类社会造成了极大的影响。据全球共享流感数据倡议组织统计,SARS-CoV-2的变异序列超过1200万种,其中影响力最大的是5种关注变异株:Alpha、Beta、Gamma、delta以及omicron。它们的传染性、致病性及中和抗性相对于原始株都有极大的改变,给疫情防控带来了极大的压力。抗体水平检测对确诊感染、流行病学调查、疫苗研发和中和药物的制备有着至关重要的意义。本文从SARS-CoV-2的体液免疫出发,简要总结了5种关注变异株的突变位点、中和抗性和疫苗效果,并简要探讨了SARS-CoV-2的进化特征、未来变异方向及宿主免疫。

关键词：新型冠状病毒关注变异株抗体疫苗中和抗体

新型冠状病毒奥密克戎变异株感染患者麻醉与围术期管理指导意见

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中华麻醉学杂志 2023年第1期43卷 15-23页

作者：新型冠状病毒奥密克戎变异株感染患者麻醉与围术期管理指导意见工作组陈向东王锷刘学胜不详华中科技大学同济医学院附属协和医院麻醉科武汉430022 中南大学湘雅医院麻醉科中南大学湘雅医院国家老年疾病临床研究中心长沙410008 安徽医科大学第一附属医院麻醉科合肥230022

2022年12月26日,国家卫生健康委员会发布公告,将新型冠状病毒(以下简称新冠病毒)肺炎更名为新冠病毒感染,并于2023年将该传染病纳入"乙类乙管"。根据世界卫生组织最新统计,截至2023年1月1日,全球共有6亿5000万余人罹患新冠病毒感染,其... 详细信息

2022年12月26日,国家卫生健康委员会发布公告,将新型冠状病毒(以下简称新冠病毒)肺炎更名为新冠病毒感染,并于2023年将该传染病纳入"乙类乙管"。根据世界卫生组织最新统计,截至2023年1月1日,全球共有6亿5000万余人罹患新冠病毒感染,其中有约660万患者不幸死亡。当下在我国流行的新冠病毒主要是奥密克戎变异株,其较之前的毒株具有更强的传播性和免疫逃逸能力[1]。目前已经有不少国内外研究表明新冠病毒感染对生理功能、对各重要器官和系统、对麻醉和围术期管理都有不同程度的影响;对于新冠病毒感染患者不同手术时机的选择也会明显影响围术期病死率和并发症发生率,因此本专家工作组集中各位专家意见形成了以下指导意见供临床工作者参考。

关键词：病毒感染专家工作组变异株器官和系统免疫逃逸传播性麻醉卫生健康

成都地区omicron变异株感染儿童的临床特征分析

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中国当代儿科杂志 2023年第8期25卷 849-854页

作者：蒋静杨梅李德渊乔莉娜张海洋四川大学华西第二医院小儿肾脏科/出生缺陷与相关妇儿疾病教育部重点实验室四川成都610041 成都市第七人民医院儿科四川成都610213 四川大学华西第二医院儿童重症医学科/出生缺陷与相关妇儿疾病教育部重点实验室四川成都610041

目的探讨成都地区新型冠状病毒omicron变异株感染患儿的临床特征。方法回顾性分析2022年8月28日—9月21日于成都市方舱医院隔离治疗的226例感染新型冠状病毒omicron变异株患儿的临床资料,根据感染过程中有无临床症状将其分为无症状感染... 详细信息

目的探讨成都地区新型冠状病毒omicron变异株感染患儿的临床特征。方法回顾性分析2022年8月28日—9月21日于成都市方舱医院隔离治疗的226例感染新型冠状病毒omicron变异株患儿的临床资料,根据感染过程中有无临床症状将其分为无症状感染组和轻症组,比较两组患儿的临床特征、诊治及转归的差异。结果226例omicron变异株感染患儿中无症状感染71例(31.4%),轻症155例(68.6%)。临床症状以发热(61.3%,95/155)、咳嗽(59.4%,92/155)最常见。188例(83.2%)儿童接种过疫苗。核酸转阴时间为6∽26 d,核酸转阴率为58.0%(131/226)。不同年龄组患儿在性别、前期症状、临床分型、核酸复阳率、核酸转阴率及住院时长等方面比较差异无统计学意义(P>0.05)。无症状感染组和轻症组患儿在年龄、性别、基础疾病、疫苗接种、连花清瘟颗粒的使用、核酸转阴率、核酸复阳率、住院时长等方面比较差异均无统计学意义(P>0.05)。结论成都地区omicron变异株感染患儿临床症状轻微,以上呼吸道感染症状为主,对各年龄段儿童健康威胁均小,总体预后良好。

关键词：新型冠状病毒 omicron变异株临床特征儿童

上海市儿童新型冠状病毒变异株感染中医药防治专家共识(2022春季版)

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上海中医药杂志 2022年第8期56卷 4-6页

作者：上海市儿童新型冠状病毒变异株感染中医药防治专家共识(2022春季版)编写组薛征不详上海中医药大学附属市中医医院

参照国家和上海市新型冠状病毒肺炎相关现行中医诊疗方案有关规定,结合2022年春季上海地区新型冠状病毒感染特点和感染奥密克戎变异株儿童大多表现为无症状、轻型或普通型等具体情况,特制定《上海市儿童新型冠状病毒变异株感染中医药防... 详细信息

参照国家和上海市新型冠状病毒肺炎相关现行中医诊疗方案有关规定,结合2022年春季上海地区新型冠状病毒感染特点和感染奥密克戎变异株儿童大多表现为无症状、轻型或普通型等具体情况,特制定《上海市儿童新型冠状病毒变异株感染中医药防治专家共识(2022春季版)》,以期提高本市儿童新型冠状病毒感染中医药防治成效。

关键词：新型冠状病毒新型冠状病毒肺炎奥密克戎变异株儿童中医药疗法专家共识

omicron株新型冠状病毒灭活疫苗(Vero细胞)原液特异性鉴别试验RT-PCR法的建立及验证

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中国生物制品学杂志 2022年第6期35卷 700-705页

作者：杨安纳庞德钦周以斯杨洁杨东升吴杰孟胜利王泽鋆郭靖申硕武汉生物制品研究所有限责任公司湖北武汉430060

目的建立omicron株新型冠状病毒灭活疫苗(Vero细胞)原液特异性鉴别试验的RT-PCR法,并对该方法进行验证。方法根据GIAIDS数据库中新型冠状病毒omicron株及其他突变株和原型株的S基因序列差异设计并合成引物OSTF1、OS1R,提取omicron株新... 详细信息

目的建立omicron株新型冠状病毒灭活疫苗(Vero细胞)原液特异性鉴别试验的RT-PCR法,并对该方法进行验证。方法根据GIAIDS数据库中新型冠状病毒omicron株及其他突变株和原型株的S基因序列差异设计并合成引物OSTF1、OS1R,提取omicron株新型冠状病毒灭活疫苗(Vero细胞)原液病毒RNA,逆转录为cDNA,利用对omicron株S蛋白基因序列中的特异性插入及缺失核苷酸序列设计的引物对进行PCR扩增,扩增产物进行1%琼脂糖凝胶电泳鉴定。对建立的RT-PCR法进行重复性、中间精密度、专属性、耐用性及灵敏度验证,并用该方法鉴别6批omicron株新型冠状病毒灭活疫苗原液。结果omicron株新型冠状病毒灭活疫苗(Vero细胞)原液利用引物OSTF1/OS1R进行RT-PCR检测,可扩增出约460 bp的条带,而原型株、Beta株、delta株新型冠状病毒灭活疫苗原液(Vero细胞)均无法扩增出条带;重复3次检测、2名实验人员分别在3 d重复3次检测、利用3种不同退火温度进行RT-PCR,omicron株新型冠状病毒灭活疫苗(Vero细胞)原液均可扩增出约460 bp的条带;原液蛋白浓度在0.04μg/mL以上、RNA浓度在0.128 ng/μL以上均可扩增出较亮的约460 bp的条带;6批omicron株新型冠状病毒灭活疫苗(Vero细胞)原液经RT-PCR检测,均可见约460 bp的条带。结论RT-PCR法专属性、重复性、中间精密度、耐用性和灵敏度均良好,可用于omicron株新型冠状病毒灭活疫苗(Vero细胞)原液鉴别。

关键词： omicron株新型冠状病毒灭活疫苗原液鉴别试验 RT-PCR