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In vivo analysis of hybrid hydrogels containing dual growth factor combinations,and skeletal stem cells under mechanical stimulation for bone repair

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Mechanobiology in Medicine 2024年第4期2卷 97-109页

作者： David Gothard Michael Rotherham Emma L.Smith Janos M.Kanczler James Henstock Julia A.Wells Carol A.Roberts omar Qutachi Heather Peto Hassan Rashidi Luis Rojo Lisa J.White Molly M.Stevens Alicia J.El Haj Felicity R.J.ose Richard O.C.Oreffo bone and Joint Research Group Centre for Human DevelopmentStem Cells and RegenerationInstitute of Developmental SciencesUniversity of SouthamptonSouthamptonSO166YDUK Institute for Science and Technology in Medicine Keele UniversityGuy Hilton Research CentreStoke-on-TrentST47BQUK Healthcare Technologies Institute School of Chemical EngineeringInstitute of Translational MedicineUniversity of BirminghamBirminghamB152THUK Department of Applied Sciences Pandon BuildingNorthumbria UniversityNewcastle-upon-TyneNE21XEUK Wolfson Centre for Stem Cells Tissue Engineering and ModellingUniversity of NottinghamCentre for Biomolecular SciencesUniversity ParkNottinghamNG72RDUK Leicester Institute for Pharmaceutical Innovation(LIPI) Leicester School of PharmacyFaculty of Health and Life SciencesDe Montfort UniversityThe GatewayLeicesterLE19BHUnited Kingdom Stem Cells and Regenerative Medicine Section UCL Great Ormond Street Institute of Child HealthUniversity College LondonLondonWC1N 1EHUK Department of Materials Imperial College LondonRoyal School of MinesLondonSW72AZUK Department of bioengineering Imperial College LondonSouth Kensington CampusLondonSW72AZUK Instituto de Ciencia y Tecnología de Polímeros(ICTP) CSICCalle Juan de la Cierva328006 MadridSpain Consorcio Centro de Investigacion biomedica en Red CIBER-BBNInstituto de Salud Carlos IIICalle Monforte de Lemos 3-51128029 PabellonMadridSpain Nottingham biodiscovery Institute School of PharmacyUniversity of NottinghamNottinghamNG72RDUK Institute for biomedical Engineering Imperial College London South Kensington CampusLondonSW72AZUK Department of Physiology Anatomy&GeneticsDepartment of Engineering Scienceand Kavli Institute for Nanoscience DiscoveryUniversity of OxfordOxfordOX13QUUK

bone tissue engineering requires a combination of materials,cells,growth factors and mechanical cues to recapitulate bone *** this study we evaluated hybrid hydrogels for minimally invasive bone formation by combining biomaterials with skeletal stem cells and staged release of growth factors together with *** hydrogels consisting of alginate and decellularized,demineralised bone extracellular matrix(ALG/ECM)were seeded with Stro-1t human bone marrow stromal cells(HbMSCs).Dual combinations of growth factors within staged-release polylactic-co-glycolic acid(PLGA)microparticles were added to hydrogels to mimic,in part,the signalling events in bone regeneration:VEGF,TGF-β_(3),PTHrP(fast release),or bMP-2,vitamin D_(3)(slow release).Mechanotransduction was initiated using magnetic fields to remotely actuate superparamagnetic nanoparticles(MNP)targeted to TREK1 ion *** hydrogels were implanted subcutaneously within mice for 28 days,and evaluated for bone formation using micro-CT and *** hydrogels lacking HbMSCs,growth factors,or MNP became mineralised,and neither growth factors,HbMSCs,nor mechanotransduction increased bone ***,structural differences in the newly-formed bone were influenced by growth *** release of bMP-2 induced thick bone trabeculae and PTHrP or VitD_(3)increased bone ***,fast-release of TGF-β_(3)and VEGF resulted in thin *** reversed the trabecular thinning and increased collagen deposition with PTHrP and VitD_(3).Our findings demonstrate the potential of hybrid ALG/ECM hydrogel–cell–growth factor constructs to repair bone in combination with mechanotransduction for fine-tuning bone *** approach may form a minimally invasive reparative strategy for bone tissue engineering applications.

关键词： Growth factors Controlled release bone ECM bone formation Mechanotransduction Skeletal stem cell

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菊苣多糖对四氧嘧啶诱导糖尿病小鼠的影响

安徽农业科学 2024年第2期52卷 156-160页

作者：阿丽米热·买买提吾买尔江·牙合甫王晓杰马雪连夏利宁姚刚新疆农业大学动物医学学院新疆乌鲁木齐830052

[目的]探究菊苣多糖对糖尿病(DM)模型小鼠肝脏氧化应激的缓解作用。[方法]采用乙醇提取法从菊苣种子中提取多糖,采用苯酚-硫酸法测定多糖,并测试DPPH自由基清除作用。采用四氧嘧啶复制小鼠DM模型,取48只DM小鼠随机分组为四氧嘧啶模型组(... 详细信息

[目的]探究菊苣多糖对糖尿病(DM)模型小鼠肝脏氧化应激的缓解作用。[方法]采用乙醇提取法从菊苣种子中提取多糖,采用苯酚-硫酸法测定多糖,并测试DPPH自由基清除作用。采用四氧嘧啶复制小鼠DM模型,取48只DM小鼠随机分组为四氧嘧啶模型组(ALX)、盐酸二甲双胍阳性组(ALX+M)、菊苣多糖低剂量组(ALX+L)、菊苣多糖高剂量组(ALX+H),每组12只;另取12只健康小鼠作为正常对照组(NC);每日灌胃给药1次,连续给药15 d。末次给药后,采集血清测定谷丙转氨酶(ALT)、谷草转氨酶(AST)、血糖、肌酐以及小鼠肝组织氧化应激指标丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽(GSH)、过氧化氢酶(CAT),观察小鼠肝、肾、胰腺、睾丸等组织病理变化。[结果]菊苣种子多糖提取率为39%,具有一定的清除DPPH自由基作用;四氧嘧啶能引起小鼠血清ALT、AST、血糖、肌酐指标上升(P<0.01),肝脏、肾脏组织发生肝细胞排列紊乱、肝小叶结构破坏、肾小球萎缩、间质增大等病理变化。菊苣多糖高剂量组(ALX+H)具有明显改善小鼠体重、脏器指数,缓解肝组织GSH、SOD、CAT水平(P<0.05),明显改善肝组织形态结构,睾丸组织病理学变化趋向NC组。[结论]菊苣多糖具有一定的抗氧化应激作用。

关键词：菊苣多糖四氧嘧啶糖尿病抗氧化应激

色素性紫癜性皮病和肝病指标:10例患者的病案报道

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世界核心医学期刊文摘（皮肤病学分册） 2005年第9期1卷 51-51页

作者： Wagdy Dessoukey M.W. Abdel-Dayem H. omar M.F. Al-Suweidi N.E. Department of Dermatology Al-Jazeera Hospital PO Box 2427 Abu Dhabi United Arab Emirates Dr.

background:Pigmented purpuric dermatosis comprises a group of vascular disorders of unknown etiology. Histologically, it is characterized by lymphocytic capillaritis in the papillary dermis. Although leukocytoclastic vasculitis confined to the skin is frequently reported with hepatitis C, lymphocytic vasculitis is rarely reported. Methods:Ten patients with pigmented purpuric dermatosis were studied clinically and histopathologically. Hepatitis profile was carried out in all of the patients to evaluate the possible relation. Results:Of the 10 patients, five tested positive for hepatitis C and two for hepatitis b antibodies. Conclusion:Hepatitis C and b virus may play a role in the pathogenesis of pigmented purpuric dermatosis. Further case-control studies are necessary to confirm this conclusion.

关键词：病案报道真皮乳头毛细血管炎乙肝病毒感染乙肝抗体丙型肝炎淋巴细胞性丙肝抗体血管疾病文献报道

Management of granulomatous lobular mastitis: an international multidisciplinary consensus(2021 edition)

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Military Medical Research 2022年第4期9卷 389-403页

作者： Qian-Qian Yuan Shu-Yuan Xiao omar Farouk Yu-Tang Du Fereshte Sheybani Qing Ting Tan Sami Akbulut Kenan Cetin Afsaneh Alikhassi Rami Jalal Yaghan Irmak Durur-Subasi Fatih Altintoprak Tae Ik Eom Fatih Alper Mustafa Hasbahceci David Martínez-Ramos Pelin Seher Oztekin Ava Kwong Cedric W.Pluguez-Turul Kirstyn Ebrownson Shirish Chandanwale Mehran Habib Liu-Yi Lan Rui Zhou Xian-Tao Zeng Jiao bai Jun-Wen bai Qiong-Rong Chen Xing Chen Xiao-Ming Zha Wen-Jie Dai Zhi-Jun Dai Qin-Yu Feng Qing-Jun Gao Run-Fang Gao bao-San Han Jin-Xuan Hou Wei Hou Hai-Ying Liao Hong Luo Zheng-Ren Liu Jing-Hua Lu bin Luo Xiao-Peng Ma Jun Qian Jian-Yong Qin Wei Wei Gang Wei Li-Ying Xu Hui-Chao Xue Hua-Wei Yang Wei-Ge Yang Chao-Jie Zhang Fan Zhang Guan-Xin Zhang Shao-Kun Zhang Shu-Qun Zhang Ye-Qiang Zhang Yue-Peng Zhang Sheng-Chu Zhang Dai-Wei Zhao Xiang-Min Zheng Le-Wei Zheng Gao-Ran Xu Wen-bo Zhou Gao-Song Wu Department of Thyroid and breast Surgery Zhongnan Hospital of Wuhan UniversityWuhan430071China Department of Pathology University of Chicago Pritzker School of MedicineChicagoIL60637USA Department of Surgical Oncology and breast Surgery Oncology CenterFaculty of MedicineMansoura UniversityMansoura35516Egypt Department of breast Surgery Beijing University of Chinese MedicineBeijing100700China Department of Infectious Diseases and Tropical Medicine Faculty of MedicineMashhad University of Medical SciencesMashhad***Iran breast Department KK Women’s and Children’s Hospital100 Bukit Timah RoadSingapore229899Singapore Department of Surgery Department of Public HealthDepartment of BiostatisticsBioinformatics and Medical InformaticsInonu University Faculty of Medicine44280MalatyaTurkey Department of General Surgery Faculty of MedicineÇanakkale Onsekiz Mart University17020ÇanakkaleTurkey Department of Radiology Cancer InstituteImam Khomeini HospitalTehran University of Medical SciencesTehran***Iran Department of Surgery College of Medicine and Medical SciencesArabian Gulf University-BahrainManama26671Bahrain Department of Radiology International Faculty of MedicineIstanbul Medipol University34810IstanbulTurkey Department of General Surgery Faculty of MedicineSakarya University54050SakaryaTurkey Department of Surgery HiU Clinic170Gwongwang-roPaldal-guSuwon16488Korea Department of Radiology Faculty of MedicineAtaturk University25240ErzurumTurkey Academic Support and Education Center Hırkai Serif DistrictKececi Cesmesi StrDoktorlar BuildingB/734091IstanbulTurkey Department of General and Digestive Surgery Hospital General CastellonAvda Benicassim S/N12812004CastellónSpain Radiology Department Ankara Training and Research Hospital305018AnkaraTurkey Department of Surgery The University of Hong KongChina The University of Hong Kong-Shenzhen Hospital Shenzhen518053China University of Miami Health System and Miller School of Medicine 1475 NW 12th AvenueM

Granulomatous lobular mastitis(GLM) is a rare and chronic benign inflammatory disease of the breast. Difficulties exist in the management of GLM for many front-line surgeons and medical specialists who care for patients with inflammatory disorders of the breast. This consensus is summarized to establish evidence-based recommendations for the management of GLM. Literature was reviewed using PubMed from January 1, 1971 to July 31, 2020. Sixty-six international experienced multidisciplinary experts from 11 countries or regions were invited to review the *** of evidence were determined using the American College of Physicians grading system, and recommendations were discussed until consensus. Experts discussed and concluded 30 recommendations on historical definitions,etiology and predisposing factors, diagnosis criteria, treatment, clinical stages, relapse and recurrence of GLM. GLM was recommended as a widely accepted definition. In addition, this consensus introduced a new clinical stages and management algorithm for GLM to provide individual treatment strategies. In conclusion, diagnosis of GLM depends on a combination of history, clinical manifestations, imaging examinations, laboratory examinations and *** approach to treatment of GLM should be applied according to the different clinical stage of GLM. This evidencebased consensus would be valuable to assist front-line surgeons and medical specialists in the optimal management of GLM.

关键词： Granulomatous mastitis Granulomatous lobular mastitis Idiopathic granulomatous mastitis Diagnosis Treatment

新疆喀什某规模化安格斯牛场犊牛产气荚膜梭菌的分离鉴定及耐药性分析

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中国畜牧兽医 2024年第5期51卷 2188-2197页

作者：马紫薇马学军马亚楠孙亚伟吾买尔江·牙合甫邵白新月杨作斌王选艺王梦娇钟旗马雪连姚刚新疆农业大学动物医学学院乌鲁木齐830000 新疆昌吉市二六工镇农业(畜牧)发展服务中心昌吉831100 新疆维吾尔自治区昌吉回族自治州农产品质量安全中心昌吉831100 新疆畜牧科学院兽医研究所乌鲁木齐830000

【目的】产气荚膜梭菌(Clostridium perfringens,CP)是一种革兰阳性厌氧菌,可导致犊牛猝死、出血性坏死性肠炎、肠毒血症等,对犊牛健康造成巨大影响。本研究旨在了解新疆喀什地区某规模化肉牛场安格斯犊牛产气荚膜梭菌流行现状及耐药情... 详细信息

【目的】产气荚膜梭菌(Clostridium perfringens,CP)是一种革兰阳性厌氧菌,可导致犊牛猝死、出血性坏死性肠炎、肠毒血症等,对犊牛健康造成巨大影响。本研究旨在了解新疆喀什地区某规模化肉牛场安格斯犊牛产气荚膜梭菌流行现状及耐药情况,为该地区由产气荚膜梭菌引起的疾病的治疗提供科学依据。【方法】试验采集20份组织样品及6份圈舍粪土样品,采用分离培养、形态学观察、染色镜检、生化试验等方法进行细菌分离鉴定,并采用K-b纸片法进行药敏试验,PCR检测分离菌的毒力型及耐药基因。【结果】分离菌在FTG液体培养基中出现浑浊并产生大量气体,在TSC培养基中长出黑色边缘整齐的圆形菌落,在绵羊血平皿上长出具有典型的双溶血环菌落,革兰染色镜检可见菌体粗短、成单个或双个排列的革兰阳性直杆菌,形态学及镜检结果符合产气荚膜梭菌特点。26份样品中共分离出10株产气荚膜梭菌,检出率为38.46%。经毒素基因分型鉴定,其中,A型产气荚膜梭菌9株,检出率为90.0%,D型产气荚膜梭菌1株,检出率为10.0%。药敏试验结果显示,分离株对卡那霉素耐药率最高,为80.0%;对庆大霉素和林可霉素耐药率为50.0%,而其对美罗培南、头孢吡肟、氟苯尼考及多西环素敏感。耐药基因检测结果表明,bla TEM基因检出率最高,为100.0%;bla SHV、qnrA和aac(6′)-Ⅰb-cr基因检出率分别为20.0%(2/10)、10.0%(1/10)和20.0%(2/10);未检出bla CTX-M和qnrS 2种耐药基因。【结论】新疆喀什地区安格斯犊牛产气荚膜梭菌感染主要以A型为主,耐药情况严重,且存在多重耐药。试验结果可为后期产气荚膜梭菌流行病学研究和防控提供科学依据。

关键词：安格斯犊牛产气荚膜梭菌分离鉴定耐药性

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菊苣多糖对小鼠药物性肝损伤保护作用的筛选

现代畜牧兽医 2021年第4期 9-13页

作者：王晓杰吾买尔江·牙合甫阿丽米热·买买提韩俊成杨莉夏利宁赛福丁·阿不拉卡哈尔·司马义新疆农业大学动物医学学院新疆乌鲁木齐830052 哈密市伊州区花园乡卫生院新疆哈密839000

试验研究对菊苣种子、根、茎及叶4个部位多糖通过含量测定及抗氧化能力试验进行保肝作用的筛选,选取最优部位多糖探究其对药物性肝损伤(DILI)的保护作用。以50 mg/kg或100 mg/kg的剂量预防性给予昆白小鼠菊苣多糖3 d,腹腔注射对乙酰氨基... 详细信息

试验研究对菊苣种子、根、茎及叶4个部位多糖通过含量测定及抗氧化能力试验进行保肝作用的筛选,选取最优部位多糖探究其对药物性肝损伤(DILI)的保护作用。以50 mg/kg或100 mg/kg的剂量预防性给予昆白小鼠菊苣多糖3 d,腹腔注射对乙酰氨基酚(APAP) 300 mg/kg诱导药物性肝损伤,测定血清、肝脏的生化指标、氧化指标和考察肝脏病理变化。结果显示,菊苣多糖呈剂量依赖性降低APAP引起血清谷丙转氨酶(ALT)、谷草转氨酶(AST)活性的升高,抵抗APAP造成的肝脏氧化损伤,提高肝脏超氧化物歧化酶(SOD)、谷胱甘肽(GSH)的活性,降低过氧化氢酶(H2O2酶)活性、丙二醛(MDA)含量,缓解APAP引起的肝脏细胞的病理变化的发生,保护肝脏抵御APAP造成的损伤。

关键词：菊苣多糖对乙酰氨基酚药物性肝损伤