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Isolation and Identification of Bacteria Found in the Milt of Cultured Clarias gariepinus

Journal of Fisheries Science 2022年第2期4卷 9-14页

作者： Folalu Adekunle Awe Ayofe Mutalib Hammed Olamiwaye Akintade Adeboyejo Olusegun Olufemi Whenu oluwatosin modupe olarinmoye Gabriel Olarinde Mekuleyi Jesuyon Joseph Hungbo Akanji Adeolu Akinyemi Olufunmilayo Adebimpe Olanloye Department of Fisheries Faculty of ScienceLagos State UniversityOjoLagos StateNigeria Department of Aquaculture and Fisheries Management College of Environmental Resources ManagementFederal University of AgricultureAbeokutaNigeria Department of Pure and Applied Botany College of BiosciencesFederal University of AgricultureAbeokutaNigeria

This study investigated bacteria that are associated with milt in cultured samples of African catfish-Clarias *** broodstocks of Clarias gariepinus were collected from private Fish Farm in Ogun State,Nigeria,while the fish’s milt was collected and analyzed for microbial load at laboratory of the Department of Microbiology,Federal University of Agriculture,Abeokuta,Ogun *** molecular characterization of the amplicon was done at International Institute of Tropical Agriculture,***-edit was used for importing and mining nucleotide sequences into gene *** revealed by the results,bacterial organisms that were present in the milt of Clarias gariepinus are Aeromonas caviae,Proteus mirabilis,Serratia rubidaea,Pseudomonas mosselii,Acinetobacter soli and Klebsiella *** Basic Local Alignment Search Tools revealed the percentage similarity ranging from 86%-97.04%and their accession *** bacteria indicated high levels of faecal contamination in the *** conclusion,bacteria were found in the milt of cultured catfish and are capable of being pathogenic to humans and may increase the vertical transfer to fry during breeding and rearing ***,fish farmers should maintain a hygiene and serene environment during breeding and culturing of catfish.

关键词： Milt Clarias gariepinus Bacteria Molecular method

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