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Signal Transduction and Targeted Therapy 2023年第10期8卷 4417-4418页

作者： Matthias Wirth Walburgis Brenner oliver h.kramer Department of hematology Oncology and Cancer ImmunologyCampus Benjamin FranklinCharite-Universitatsmedizin Berlincorporate member of Freie Universitat Berlin and Humboldt-Universitat zu BerlinBerlinGermany University Medical Center Gottingen Department of GeneralVisceral and Pediatric SurgeryGottingenGermany German Cancer Consortium(DKTK) German Cancer Research Center(DKFZ)HeidelbergGermany Clinic for Obstetrics and Women's health University Medical CenterJohannes Gutenberg University MainzLangenbeckstr.155131 MainzGermany Department of Toxicology University Medical CenterMainzGermany

In a recent publication in Cell,hasegawa and colleagues reveal that cytotoxic CD4-positive cytotoxic T lymphocytes(CD4+CTLs)eliminate senescent fibroblasts with a surface expression of the immunologically important human leukocyte antigen class Il(hLA-)and the human cytomegalovirus glycoprotein B(hCMV-gB).1 This implies that a virome-immune cell interaction controls the fate of human skin cells that are linked to the aging process and associated diseases.

关键词： immunity cytomegalovirus cytotoxic

Establishment,functional and genetic characterization of a colon derived large cell neuroendocrine carcinoma cell line

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World Journal of Gastroenterology 2018年第33期24卷 3749-3759页

作者： Michael Gock Christina S Mullins Christine harnack Friedrich Prall Robert Ramer Anja Goder oliver h kramer Ernst Klar Michael Linnebacher Department of General Surgery University of RostockRostock 18055Germany Department of General Surgery Section of Molecular Oncology and ImmunotherapyUniversity of RostockRostock 18055Germany Institute of Pathology University of RostockRostock 18055Germany Institute of Pharmacology University of RostockRostock 18055Germany Institute of Toxicology University Medical Center MainzMainz 55131Germany

AIM To establish cell line and patient-derived xenograft(PDX) models for neuroendocrine carcinomas(NEC) which is highly desirable for gaining insight into tumor development as well as preclinical research includingbiomarker testing and drug response *** Cell line establishment was conducted from direct in vitro culturing of colonic NEC tissue(hROC57). A PDX could also successfully be established from vitally frozen tumor samples. Morphological features, invasive and migratory behavior of the hROC57 cells as well as expression of neuroendocrine markers were vastly analyzed. Phenotypic analysis was done by microscopy and multicolor flow cytometry. The extensive molecular-pathological profiling included mutation analysis, assessment of chromosomal and microsatellite instability; and in addition, fingerprinting(i.e., STR analysis) was performed from the cell line in direct comparison to primary patient-derived tissues and the PDX model established. Drug responsiveness was examined for a panel of chemotherapeutics in clinical use for the treatment of solid *** The established cell line hROC57 showed distinct morphological and molecular features of a poorly differentiated large-cell NEC with KI-67 > 50%. Molecular-pathological analysis revealed a Cp G island promoter methylation positive cell line with microsatellite instability being absent. The following mutation profile was observed: KRAS(wt), BRAF(mut). A high sensitivity to etoposide, cisplatin and 5-FU could be demonstrated while it was more resistant towards rapamycin. CONCLUSION We successfully established and characterized a novel patient-derived NEC cell line in parallel to a PDX model as a useful tool for further analysis of the biological characteristics and for development of novel diagnostic and therapeutic options for NEC.

关键词： Patient-derived tumor model Large cell neuroendocrine carcinoma Individualized medicine

Establishment,functional and genetic characterization of three novel patient-derived rectal cancer cell lines

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World Journal of Gastroenterology 2018年第43期24卷 4880-4892页

作者： Michael Gock Christina S Mullins Carina Bergner Friedrich Prall Robert Ramer Anja Goder oliver h kramer Falko Lange Bernd J Krause Ernst Klar Michael Linnebacher Department of General Surgery University Medical CenterRostock 18055Germany Section of Molecular Oncology and Immunotherapy University MedicalCenterRostock 18055Germany Department of NuclearMedicine University Medical CenterRostock 18055Germany Institute of Pathology University MedicalCenterRostock 18055Germany Institute of Pharmacology University Medical CenterRostock 18055Germany Institute of Toxicology University Medical Center MainzMainz 55131Germany Oscar-Langendorff-Institute of Physiology University Medical CenterRostock 18055Germany

AIM To establish patient-individual tumor models of rectal cancer for analyses of novel biomarkers, individual response prediction and individual therapy *** Establishment of cell lines was conducted by direct in vitro culturing and in vivo xenografting with subsequent in vitro culturing. Cell lines were in-depth characterized concerning morphological features, invasive and migratory behavior, phenotype, molecular profile including mutational analysis, protein expression, and confirmation of origin by DNA fingerprint. Assessment of chemosensitivity towards an extensive range of current chemotherapeutic drugs and of radiosensitivity was performed including analysis of a combined radioand chemotherapeutic treatment. In addition, glucose metabolism was assessed with 18 F-fluorodeoxyglucose(FDG) and proliferation with 18 *** We describe the establishment of ultra-low passage rectal cancer cell lines of three patients suffering from rectal cancer. Two cell lines(hROC126, hROC284 Met) were established directly from tumor specimens while hROC239 T0 M1 was established subsequent to xenografting of the tumor. Molecular analysis classified all three cell lines as CIMP-0/non-MSI-h(sporadic standard) type. Mutational analysis revealed following mutational profiles: hROC126: APC^(wt), TP53^(wt), KRAS^(wt), BRAF^(wt), PTEN^(wt); hROC239 T0 M1: APC^(mut), P53^(wt), KRAS^(mut), BRAF^(wt), PTEN^(mut) and hROC284 Met: APC^(wt), P53^(mut), KRAS^(mut), BRAF^(wt), PTEN^(mut). All cell lines could be characterized as epithelial(EpCAM+) tumor cells with equivalent morphologic features and comparable growth kinetics. The cell lines displayed a heterogeneous response toward chemotherapy, radiotherapy and their combined application. hROC126 showed a highly radio-resistant phenotype and hROC284 Met was more susceptible to a combined radiochemotherapy than hROC126 and hROC239 T0 M1. Analysis of 18 F-FDG uptake displayed a markedly reduced FDG uptake of all th

关键词： Patient-derived tumor model Rectal cancer ^(18)F-fluorodeoxyglucose ^(18)F-fluorothymidine FOLFOX FOLFIRI Personalized medicine

Controlling the reaction network of Ni/silica derived conversion of bio-oil surrogate guaiacol

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Carbon Resources Conversion 2024年第1期7卷 39-60页

作者： Nils Kretzschmar oliver Busse Markus Seifert Technische Universität Dresden Faculty of Chemistry and Food Chemistry01062 DresdenGermany

Replacing fossil carbon sources with green bio-oils is a promising route to switch to a sustainable chemical industry,although their high oxygen contents are *** hydrodeoxygenation is a favored route to upgrade bio-oils to renewable fuels and basic *** this work,we investigated Ni/SiO2 catalysts with differing metal dispersity in continuous mode conversion of guaiacol with a statistical experimental design for 250℃to 400℃,2 h up to 5 h time on stream(ToS)and subsequently different residence time besides other *** low temperature(250℃)promotes cyclohexanol formation from guaiacol,high temperature(400℃)inhibits hydrogenation,leading to phenol and *** medium temperature(340℃),the selectivity for cyclohexanone *** and cyclohexanone(KA-oil)are the industrial basis for polyamide ***,we clarified the role of 2-methoxycyclohexanol(2MC)in the reaction network towards KA-oil for continuous-mode *** analysis was used to predict and optimize product selectivity and yield,leading to the best yield of cyclohexanone/-ol at 327.5℃,low ToS,medium residence time,high particle dispersity,and medium hydrogen pressure(15 bar(g)).

关键词： hydrodeoxygenation Design of experiments Bio-oil Nickel silica catalyst Continuous mode flow reactor KA-oil Cyclohexanol Cyclohexanone

Entangled photon pair generation in an integrated SiC platform

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Light(Science & Applications) 2024年第6期13卷 1041-1046页

作者： Anouar Rahmouni Ruixuan Wang Jingwei Li Xiao Tang Thomas Gerrits oliver Slattery Qing Li Lijun Ma National Institute of Standards and Technology 100 Bureau DrGaithersburgMD20899USA Department of Electrical and Computer Engineering Carnegie Mellon UniversityPittsburghPA15213USA

Entanglement plays a vital role in quantum information *** to its unique material properties,silicon carbide recently emerged as a promising candidate for the scalable implementation of advanced quantum information processing *** date,however,only entanglement of nuclear spins has been reported in silicon carbide,while an entangled photon source,whether it is based on bulk or chip-scale technologies,has remained ***,we report the demonstration of an entangled photon source in an integrated silicon carbide platform for the first ***,strongly correlated photon pairs are efficiently generated at the telecom C-band wavelength through implementing spontaneous four-wave mixing in a compact microring resonator in the 4h-silicon-carbide-on-insulator *** maximum coincidence-to-accidental ratio exceeds 600 at a pump power of 0.17 mW,corresponding to a pair generation rate of(9±1)×10^(3) pairs/***-time entanglement is created and verified for such signal-idler photon pairs,with the two-photon interference fringes exhibiting a visibility larger than 99%.The heralded single-photon properties are also measured,with the heralded g^((2))(0)on the order of 10^(−3),demonstrating the SiC platform as a prospective fully integrated,complementary metal-oxide-semiconductor compatible single-photon source for quantum applications.

关键词： quantum coincidence mixing

Photometric and Spectroscopic Study of Ten Low Mass Ratio Contact Binary Systems:Orbital Stability,O'Connell Effect and Infrared Calcium Line Filling

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Research in Astronomy and Astrophysics 2024年第8期24卷 212-222页

作者： Surjit S.Wadhwa Adam Popowicz Raúl Michel Petar Kostić oliver Vince Nick F.h.Tothill Ain Y.De horta Miroslav D.Filipović School of Science Western Sydney UniversityLocked Bag 1797PenrithNSW 2751Australia Department of Electronics Electrical Engineering and MicroelectronicsSilesian University of TechnologyAkademicka 1644120 GliwicePoland Instituto de Astronomía UNAM.A.P.87722800 EnsenadaBCMéxico Astronomical Observatory Volgina 711060 BelgradeSerbia

Low mass ratio contact binary systems are more likely to have unstable orbits and potentially *** addition,such systems exhibit characteristics such as starspots and high energy emissions(UV)suggestive of chromospheric and magnetic *** curve modeling of ten contact binary systems is *** were found to be of extreme low mass ratio ranging from 0.122 to 0.24 and three were found to be potentially unstable and possible merger *** of the infrared calcium absorption lines is a marker of increased chromospheric *** use the available Large Sky Area Multi-Object Fiber Spectroscopic Telescope spectra along with matched standard spectra(broadened for rotation)to measure the excess filling of the central core depression flux of the two main infrared calcium absorption linesλ8542 andλ*** find that all reported contact binaries have excess filling of the core flux in the infrared calcium *** of the systems reported were also observed by the Galaxy Evolution Explorer mission and we find that all three have features of excess ultraviolet emissions further adding evidence for increased chromospheric activity in low mass ratio contact *** of both orbital stability and absorption line filling is dependent on the determination of geometric and absolute parameters from light curve *** an insignificant number of contact binary light curves exhibit the O’Connell effect,usually attributed to *** discuss the inclusion of starspots in light curve solutions and how they influence the geometric and absolute parameters.

关键词： (stars)binaries eclipsing-techniques photometric-stars chromospheres

Utilizing neural networks to supplant chemical kinetics tabulation through mass conservation and weighting of species depletion

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Energy and AI 2024年第2期16卷 101-110页

作者： Franz M.Rohrhofer Stefan Posch Clemens Gößnitzer JoséM.García-oliver Bernhard C.Geiger Know-Center Gmbh Sandgasse 36Graz8010StyriaAustria LEC Gmbh Inffeldgasse 19Graz8010StyriaAustria Universitat Politècnica de València Camíde VeraValència46022ValènciaSpain

Artificial Neural Networks(ANNs)have emerged as a powerful tool in combustion simulations to replace memory-intensive tabulation of integrated chemical *** reaction mechanisms,however,present a challenge for standard ANN approaches as modeling multiple species typically suffers from inaccurate predictions on minor *** paper presents a novel ANN approach which can be applied on complex reaction mechanisms in tabular data form,and only involves training a single ANN for a complete reaction *** approach incorporates a network architecture that automatically conserves mass and employs a particular loss weighting based on species *** modifications are used to improve the overall ANN performance and individual prediction accuracies,especially for minor species mass *** validate its effectiveness,the approach is compared to standard ANNs in terms of performance and ANN *** distinct reaction mechanisms(h_(2),C_(7)h_(16),C_(12)h_(26),OME_(34))are used as a test cases,and results demonstrate that considerable improvements can be achieved by applying both modifications.

关键词： Neural network approach Chemical kinetics Flamelet tabulation Mass conservation Species loss weighting

On Unsupervised Training of Multi-Class Regularized Least-Squares Classifiers

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Journal of Computer Science & Technology 2014年第1期29卷 90-104页

作者： Tapio Pahikkala Antti Airola Fabian Gieseke oliver kramer ACM IEEE Department of Information Technology University of Turku Department of Computer Science University of Copenhagen Computer Science Department Carl von Ossietzky University of Oldenburg

In this work we present the first efficient algorithm for unsupervised training of multi-class regularized least- squares classifiers. The approach is closely related to the unsupervised extension of the support vector machine classifier known as maximum margin clustering, which recently has received considerable attention, though mostly considering the binary classification case. We present a combinatorial search scheme that combines steepest descent strategies with powerful meta-heuristics for avoiding bad local optima. The regularized least-squares based formulation of the problem allows us to use matrix algebraic optimization enabling constant time checks for the intermediate candidate solutions during the search. Our experimental evaluation indicates the potential of the novel method and demonstrates its superior clustering performance over a variety of competing methods on real world datasets. Both time complexity analysis and experimental comparisons show that the method can scale well to practical sized problems.

关键词： unsupervised learning multi-class regularized least-squares classification maximum margin clustering combinatorial optimization

ON KARUSh-KUhN-TUCKER POINTS FOR A SMOOThING METhOD IN SEMI-INFINITE OPTIMIZATION

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Journal of Computational Mathematics 2006年第6期24卷 719-732页

作者： oliver Stein Department o.f Mathematics-C RWTH Aachen University Germany

We study the smoothing method for the solution of generalized semi-infinite optimization problems from （O. Stein, G. Still： Solving semi-infinite optimization problems with interior point techniques, SIAM J. Control Optim., 42（2003）, pp. 769-788）. It is shown that Karush-Kuhn-Tucker points of the smoothed problems do not necessarily converge to a Karush-Kuhn-Tucker point of the original problem, as could be expected from results in （F. Facchinei, h. Jiang, L. Qi： A smoothing method for mathematical programs with equilibrium constraints, Math. Program., 85（1999）, pp. 107-134）. Instead, they might merely converge to a Fritz John point. We give, however, different additional assumptions which guarantee convergence to Karush-Kuhn：Tucker points.

关键词： Generalized semi-infinite optimization Stackelberg game Constraint qualifi-cation Smoothing NCP function.

Λb^0-baryon production in pp collisions in the general-mass variable-flavour-number scheme,and comparison with CMS and LhCb data

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Chinese Physics C 2018年第8期42卷 34-44页

作者： G.kramer h.Spiesberger II.Institut für Theoretische Physik Universitat HamburgLuruper Chaussee 149D-22761 HamburgGermany Institut für Physik Johannes-Gutenberg-UniversitatStaudinger Weg 7D-55099 MainzGermany Centre for Theoretical and Mathematical Physics and Department of Physics University of Cape TownRondebosch 7700South Africa

We calculate the next-to-leading-order cross section for the inclusive production ofΛ（b ）baryons in pp collisions in the general-mass variable-flavor-number *** use realistic evolved non-perturbative fragmentation functions obtained from fits to B-meson production in e^＋e^-annihilation and compare our results for transversemomentum and rapidity distributions with recent experimental data from the CMS and the LhCb collaborations at the CERN *** find satisfactory agreement in general,with some indication for the need to modify the available fragmentation functions at larger values of the scale variable.

关键词： heavy flavor production perturbative QCD Ab production