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The development and application of a multiplex short tandem repeat(STR)system for identifying subspecies,individuals and sex in tigers

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Integrative Zoology 2015年第4期10卷 376-388页

作者： Zheng-Ting ZOU olga V.uphyrkina Pavel FOMENKO Shu-Jin LUO College of Life Sciences Peking-Tsinghua Center for Life SciencesPeking UniversityBeijingChina Institute of Biology and Soil Sciences Far Eastern Branch Russian Academy of SciencesVladivostokRussia World Wide Fund for Nature–Russia Amur Branch VladivostokRussia Department of Computational Medicine and Bioinformatics University of MichiganAnn ArborMichiganUSA.

Poaching and trans-boundary trafficking of tigers and body parts are threatening the world’s last remaining wild *** of an efficient molecular genetic assay for tracing the origins of confiscated specimens will assist in law enforcement and wildlife forensics for this iconic flagship *** developed a multiplex genotyping system“tigrisPlex”to simultaneously assess 22 short tandem repeat(STR,or microsatellite)loci and a gender-identifying SRY gene,all amplified in 4 reactions using as little as 1 ng of template *** DNA samples used for between-run calibration,the system generates STR genotypes that are directly compatible with voucher tiger subspecies genetic profiles,hence making it possible to identify subspecies via bi-parentally inherited *** applied“tigrisPlex”to 12 confiscated specimens from Russia and identified 6 individuals(3 females and 3 males),each represented by duplicated samples and all designated as Amur tigers(Panthera tigris altaica)with high *** STR multiplex system can serve as an effective and versatile approach for genetic profiling of both wild and captive tigers as well as confiscated tiger products,fulfilling various conservation needs for identifying the origins of tiger samples.

关键词： Amur tiger genetic multiplex sexing short tandem repeat

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Canine distemper virus as a threat to wild tigers in Russia and across their range

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Integrative Zoology 2015年第4期10卷 329-343页

作者： Martin GILBERT Svetlana V.SOUTYRINA Ivan V.SERYODKIN Nadezhda SULIKHAN olga V.uphyrkina Mikhail GONCHARUK Louise MATTHEWS Sarah CLEAVELAND Dale G.MIQUELLE Wildlife Conservation Society BronxNew YorkUSA Boyd Orr Centre for Population and Ecosystem Health Institute of BiodiversityAnimal Health and Comparative MedicineCollege of MedicalVeterinary and Life SciencesUniversity of GlasgowGlasgowUK Pacific Geographical Institute Far Eastern Branch of the Russian Academy of SciencesVladivostokRussia Far Eastern Federal University VladivostokRussia Institute of Biology and Soil Sciences Far Eastern Branch of the Russian Academy of SciencesVladivostokRussia Lazovskii State Nature Zapovednik LazoPrimorskii KraiRussia

Canine distemper virus(CDV)has recently been identified in populations of wild tigers in Russia and *** populations are generally too small to maintain CDV for long periods,but are at risk of infections arising from more abundant susceptible hosts that constitute a reservoir of *** CDV is an additive mortality factor,it could represent a significant threat to small,isolated tiger *** Russia,CDV was associated with the deaths of tigers in 2004 and 2010,and was coincident with a localized decline of tigers in Sikhote-Alin Biosphere Zapovednik(from 25 tigers in 2008 to 9 in 2012).Habitat continuity with surrounding areas likely played an important role in promoting an ongoing *** recommend steps be taken to assess the presence and the impact of CDV in all tiger range states,but should not detract focus away from the primary threats to tigers,which include habitat loss and fragmentation,poaching and retaliatory *** priorities include:(i)recognition and diagnosis of clinical cases of CDV in tigers when they occur;and(ii)collection of baseline data on the health of wild *** infection of individual tigers need not imply a conservation threat,and modeling should complement disease surveillance and targeted research to assess the potential impact to tiger populations across the range of ecosystems,population densities and climate extremes occupied by *** the role of domestic and wild carnivores as contributors to a local CDV reservoir is an important precursor to considering control measures.

关键词： canine distemper virus conservation threat extinction Panthera tigris altaica population decline

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全世界老虎的自然历史与适应的全基因组进化分析

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科学新闻 2019年第2期 118-118页

作者： Yue-Chen Liu Xin Sun Carlos Driscoll Dale G.Miquelle Xiao Xu Paolo Martelli olga uphyrkina James L.D.Smith Stephen J.O'Brien 罗述金北京大学生命科学学院

文章简介几十年来,对于如何划分虎的亚种人们一直争论不止,这在一定程度上影响了世界虎保护和繁育政策的实施。而解决这些争议的关键在于全基因组信息。为了深入了解虎的演化史以及遗传多样性格局,本研究选取了32份确定地理来源的样本... 详细信息

文章简介几十年来,对于如何划分虎的亚种人们一直争论不止,这在一定程度上影响了世界虎保护和繁育政策的实施。而解决这些争议的关键在于全基因组信息。为了深入了解虎的演化史以及遗传多样性格局,本研究选取了32份确定地理来源的样本进行了平均覆盖深度为10倍的全基因组测序,共找到约180万个多态性位点。

关键词：基因组进化全世界

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