检索结果-南通市图书馆

Novel screening test for celiac disease using peptide functionalised gold nanoparticles

World Journal of Gastroenterology 2018年第47期24卷 5379-5390页

作者： Anantdeep Kaur olga shimoni Michael Wallach Institute for Biomedical Materials and Devices Faculty of Science University of Tech-nology Sydney School of Life Sciences Faculty of Science University of Technology Sydney

AIM To develop a screening test for celiac disease based on the coating of gold nanoparticles with a peptide sequence derived from gliadin, the protein that triggers celiac ***20 nm gold nanoparticles were first coated with NeutrAvidin. A long chain Polyethylene glycol(PEG) linker containing Maleimide at the Ω-end and Biotin group at the α-end was used to ensure peptide coating to the gold nanoparticles. The maleimide group with the thiol(-SH) side chain reacted with the cysteine amino acid in the peptide sequence and the biotinylated and PEGylated peptide was added to the NeutrAvidin coated gold nanoparticles. The peptide coated gold nanoparticles were then converted into a serological assay. We used the peptide functionalised gold nanoparticle-based assay on thirty patient serum samples in a blinded assessment and compared our results with the previously run serologicaland pathological tests on these *** A stable colloidal suspension of peptide coated gold nanoparticles was obtained without any aggregation. An absorbance peak shift as well as color change was caused by the aggregation of gold nanoparticles following the addition of anti-gliadin antibody to peptide coated nanoparticles at levels associated with celiac disease. The developed assay has been shown to detect anti-gliadin antibody not only in quantitatively spiked samples but also in a small-scale study on real non-hemolytic celiac disease patient's *** The study demonstrates the potential of gold nanoparticlepeptide based approach to be adapted for developing a screening assay for celiac disease diagnosis. The assay could be a part of an exclusion based diagnostic strategy and prove particularly useful for testing high celiac disease risk populations.

关键词： Celiac disease Serological point-of-care Gold nanoparticles Diagnostic test Autoantibodies

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A longitudinal assessment of retinal function and structure in the APP/PS1 transgenic mouse model of Alzheimer’s disease

引用

Translational Neurodegeneration 2019年第1期8卷 372-381页

作者： Dana Georgevsky Stephanie Retsas Newsha Raoufi olga shimoni S.Mojtaba Golzan Vision Science group Graduate School of Health(Orthoptics Discipline)University of Technology Sydney15 BroadwayUltimoSydneyNSW 2007Australia Institute of Biomedical Materials&Devices(IBMD) Faculty of ScienceUniversity of Technology Sydney15 BroadwayUltimoSydneyNSW 2007Australia

Background:A great body of evidence suggests that there are retinal functional and structural changes that occur in Alzheimer’s disease(AD).However,whether such changes are primary or secondary remains to be *** studied a range of retinal functional and structural parameters in association with AD-specific pathophysiological markers in the double transgenic APP/PS1 and control mice across ***:Electroretinogram(ERG)and optical coherence tomography(OCT)was performed in APP/PS1 and wild type(WT)control mice every 3 months from 3 to 12 months of *** functional assessment,the a-and b-wave of the ERG,amplitude of oscillatory potentials(OP)and the positive scotopic threshold response(pSTR)were quantified at each time *** structural assessment,the inner and outer retinal thickness was segmented and measured from OCT *** memory was evaluated at 6,9 and 12 months of age using the novel object recognition *** beta(Aβ)distribution in the hippocampus and the retina were visualised at 3,6 and 12 months of *** intra-group analysis was performed to study rate of change for each parameter between the two ***:Inter-group analysis revealed a significant difference in b-wave and OPs of APP/PS1 compared to WT controls starting from 3 months(p<0.001).There was also a significant difference in the amplitude of pSTR between the two groups starting from 6 months(p<0.001).Furthermore,a significant difference in the inner retinal thickness,between the two groups,was observed starting from 9 months(p<0.001).Conclusions:We observed an age-related decline in retinal functional and structural parameters in both APP/PS1 and WT controls,however,inter-group analysis revealed that inner retinal functional and structural decline is exacerbated in APP/PS1 mice,and that retinal functional changes precede structural changes in this *** studies are required to confirm whether such phenomenon occurs in humans and if studying ret

关键词： Retinal function Retinal structure Retinal imaging Biomarkers Alzheimer’s disease Early assessment

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Microscopic inspection and tracking of single upconversion nanoparticles in living cells

引用

Light(Science & Applications) 2018年第1期7卷 1034-1039页

作者： Fan Wang Shihui Wen Hao He Baoming Wang Zhiguang Zhou olga shimoni Dayong Jin Institute for Biomedical Materials and Devices(IBMD) Faculty of ScienceUniversity of Technology SydneySydneyNew South Wales 2007Australia

Nanoparticles have become new tools for cell biology imaging1,sub-cellular sensing2,super-resolution imaging3,4 and drug ***-term 3D tracking of nanoparticles and their intracellular motions have advanced the understanding of endocytosis and exocytosis as well as of active transport processes6–*** sophisticated operation of correlative optical-electron microscopy9,10 and scientific-grade cameras is often used to study intercellular ***,most of these studies are still limited by the insufficient sensitivity for separating a single nanoparticle from a cluster of nanoparticles or their aggregates8,11,*** we report that our eyes can track a single fluorescent nanoparticle that emits over 4000 photons per 100 milliseconds under a simple microscope *** tracking a single nanoparticle with high temporal,spectral and spatial resolution,we show the measurement of the local viscosity of the intracellular ***,beyond the colour domain and 3D position,we introduce excitation power density as the fifth dimension for our eyes to simultaneously discriminate multiple sets of single nanoparticles.

关键词： nanoparticles separating excitation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：