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Cytotoxic Properties on Cervical and Liver Cancer Cells of Two Plant Recipes from Burkina Faso

Journal of Biosciences and medicines 2024年第6期12卷 216-229页

作者： Estelle Ouédraogo Bagora Bayala Théodora mahoukèdè Zohoncon Samiratou Ouédraogo olga mélanie lompo Essi Etonam Dovo Jean-marie Compaoré Albert Théophane Yonli Jacques Simpore Laboratory of molecular Biology and Genetics (LABIOGENE) Joseph KI-ZERBO University Ouagadougou Burkina Faso Center of Biomolecular Research Pietro Annigoni (CERBA) Ouagadougou Burkina Faso Ecole Normale Suprieure Koudougou Burkina Faso Hspital Saint Camille of Ouagadougou (HOSCO) Ouagadougou Burkina Faso Faculty of Health University Saint Thomas dAquin (USTA) Ouagadougou Burkina Faso Chair Research and Action against Cancer Department of Public Health Training and Research Unit in Health Sciences Joseph KI-ZERBO University Ouagadougou Burkina Faso National Population Health Observatory National Institute of Public Health Ouagadougou Burkina Faso The Department of Epidemiology Biostatistics and Occupational Health School of Population and Global Health Montral Canada Laboratory of Pathological Anatomy and Cytology University Joseph KI-ZERBO Ouagadougou Burkina Faso TIIG LA TIIm Ouagadougou Burkina Faso

Cancer is one of the deadliest diseases in developing countries. In recent years, natural plant-based compounds have been used in the search for drugs to combat numerous diseases, including cancer. In this study, we evaluate the cytotoxic properties of paanfo tiben 1 and paanfo tiben 2, two traditional herbal formulations from Burkina Faso used in the treatment of cancer in Burkina Faso. To this end, the recipes were infused and freeze-dried. The dry extracts obtained were used to determine total phenolics and flavonoids content, assess antioxidant activity using the DPPH, ABTS and FRAP methods, evaluate anti-inflammatory properties by inhibiting 15-LOX, COX 1 and 2, and assess cytotoxic activity on HeLa cervical cancer and HePG2 liver cancer cell lines using the mTT test. The paanfo tiben 1 recipe showed the highest levels of total phenolics and flavonoids, as well as the best antioxidant activities, with IC50 values of 21.020 ± 0.6 µg/ml and 22.94 ± 0.57 µg/ml for DPPH and ABTS, and 165.15 mm EAA/mg dry extract for FRAP. It also exhibited the best cytotoxic activity with IC50 values of 112.02 ± 0.025 µg/ml on HeLa cells and 80.67 ± 6.08 µg/ml on HepG2 cells. On the other hand, paanfo tiben 2 exhibited the best anti-inflammatory activities through inhibition of 15-LOX and COX 1, with inhibition percentages at 100 µg/ml of 32.523% and 24.717 % respectively. These results could justify the traditional use of these two recipes by traditional health practitioners in the treatment of cancer sufferers in Burkina Faso.

关键词： Antioxidant Anti-Inflammatory Cytotoxicity Paanfo Tiben Burkina

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HPV/HBV or HPV/HCV Co-Infections in Women Treated for Chronic Hepatitis at Hôpital Saint Camille in Ouagadougou, Burkina Faso

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American Journal of molecular Biology 2024年第1期14卷 1-12页

作者： Estelle Ouédraogo Théodora mahoukèdè Zohoncon Bagora Bayala Prosper Bado Rose P. Clémence Da Rogomenoma Alice Ouedraogo Ina marie Angèle Traoré Punya Akouélé Kuassi-Kpede Samiratou Ouédraogo Essi Etonam Dovo Lassina Traoré Albert Théophane Yonli Florencia Wendkuuni Djigma olga mélanie lompo Jacques Simpore Laboratory of molecular Biology and Genetics (LABIOGENE) University Joseph KI-ZERBO Ouagadougou Burkina Faso Centre of Biomolecular Research Pietro Annigoni (CERBA) Ouagadougou Burkina Faso Hô spital Saint Camille of Ouagadougou (HOSCO) Ouagadougou Burkina Faso Faculty of Health University Saint Thomas d’Aquin (USTA) Ouagadougou Burkina Faso Ecole Normale Supérieure Koudougou Burkina Faso Chair “Research and Action Against Cancer” Département de Santé Publique Training and Research Unit in Health Sciences University Joseph KI-ZERBO Ouagadougou Burkina Faso Observatoire National de la Santé de la Population Institut National de Santé Publique Ouagadougou Burkina Faso The Department of Epidemiology Biostatistics and Occupational Health School of Population and Global Health Montreal Canada Laboratory of Pathological Anatomy and Cytology University Joseph KI-ZERBO Ouagadougou Burkina Faso

Introduction: Cervical cancer is a public health concern and is mainly caused by Human papillomaviruses (HPV). In many parts of the world, studies are being carried out to understand the different genotypes to better tackle this issue. We conducted a study to determine the prevalence of HPV genotypes in women with chronic hepatitis B or C infection, co-infected or not with HIV, treated at the Hôpital Saint Camille in Ouagadougou (Burkina Faso). methods: This study was conducted from April to July 2023, including 100 women in gastroenterology at Hôpital Saint Camille. A questionnaire on their socio-demographic and life style was administrated;and endocervical samples were collected using sterile swabs and then sent to Centre of Biomolecular Research Pietro Annigoni (CERBA). HPV molecular detection and genotyping were performed by PCR and hybridization using the HPV Direct Flow Chips kit. Data were analysis using chi square test or Fischer’s exact test with a significance threshold for p Results: The prevalence of HPV infection was 28% (28/100) on the sample of women tested. The most frequent genotypes were HPV 52 (8.33%), followed by HPV 18 and 68 (6.25% each) for high-risk HPVs, and HPV 6, 44/55 and 62/81 (8.33% each) for low-risk HPVs. Conclusion: This study, the first of its kind in Burkina Faso on this group of the population, reveals that the most frequent genotypes found in this study are not included in the vaccine available in Burkina Faso (Gardasil®4).

关键词： Human Papillomavirus Cervical Cancer Hepatitis Human Immunodeficiency Virus Burkina Faso

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Pneumatosis Cystoides Intestinalis Revealed by an Occlusive Syndrome: A Case Report

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Open Journal of Pathology 2023年第4期13卷 210-216页

作者： Franck Auguste Hermann Adémayali Ido Aimé Sosthène Ouédraogo Ibrahim Savadogo Rakiswendé Alexis Ouédraogo Souleymane Ouattara Assita Lamien-Sanou olga mélanie lompo Department of Anatomy and Pathological Cytology CHU Tengandogo Ouagadougou Burkina Faso Department of Anatomy and Pathological Cytology CHU Bogododgo Ouagadougou Burkina Faso Department of Anatomy and Pathological Cytology CHU-R of Ouahigouya Ouahigouya Burkina Faso Histo-Embryo-Cytogenetics Department Bogododgo University Hospital Ouagadougou Burkina Faso Department of Anatomy and Pathological Cytology CHU Yalgado Ouédraogo Ouagadougou Burkina Faso

Introduction: Pneumatosis cystoides intestinalis is a rare condition characterized by the presence of gas cysts in the intestinal wall. It can affect different segments of the digestive tract. male patients over 50 years old seem to be more affected. Classically, one opposes the primary or idiopathic forms and the secondary forms. Case Report: We report the case of a 62-year-old female patient with a history of treated peptic ulcers, who presented with episodes of intermittent atypical abdominal pain that worsened abruptly. The physical examination revealed a surgical abdomen with tenderness to palpation. The patient underwent a jejunal resection. The pathological examination showed macroscopically an intestinal fragment with confluent subserosal and intestinal intraparietal bullae. On opening, the portion containing the bullae was thickened, infarcted, congestive, hemorrhagic, and brittle with narrowing of the intestinal lumen. The bullae are tense and leave serosities. On histological examination, the intestinal wall was very congestive with hemorrhagic patches of suffusion. There were optically empty cystic formations that do not communicate with the digestive lumen. The adjacent mucosa is the site of a moderate subacute inflammatory reaction. Conclusion: Pneumatosis cystoides intestinalis is a rare and usually benign pathology. The symptoms, when they exist, are not specific. Its physiopathology is still very much debated. The prognosis is generally good. The knowledge of this pathology is necessary to avoid invasive explorations and heavy treatment without interest for the patient.

关键词： Cysts Gas Intestinal Pathology

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A Case of Cerebral Aspergillosis in a Patient with Chronic Lymphoid Leukemia

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Open Journal of Pathology 2023年第4期13卷 217-223页

作者： Aimé Sosthène Ouédraogo Franck Auguste Hermann Adémayali Ido Ibrahim Savadogo Souleymane Ouattara Rakiswendé Alexis Ouédraogo Assita Lamien Sanou olga mélanie lompo Pathological Anatomy Service of Bogodogo Teaching University Hospital Ouagadougou Burkina Faso UFR/SDS Joseph Ki-Zerbo University Ouagadougou Burkina Faso Pathological Anatomy Service of Tingandogo Ouagadougou Burkina Faso Pathological Anatomy Service of Ouahigouya Ouahigouya Burkina Faso Embryology Cytogenetics and Reproductive Biology of Bogodogo Teaching University Hospital Ouagadougou Burkina Faso Pathological Anatomy Service of Yalgado Ouédraogo Ouagadougou Burkina Faso

Cerebral aspergillosis commonly affects immunocompromised hosts, primarily patients on immunosuppressive therapy and those with acquired or immunodeficient states such as AIDS. We report a case of cerebral aspergillosis in a 39-year-old woman with a history of treated chronic lymphocytic leukemia (CLL). multiple fragments of fixed tissues with formalin were freshly sent to the laboratory. An extemporaneous examination was performed by spreading. The rest of the sample was embedded in paraffin and studied according to the standard histological method with special stains and an immunohistochemical study. A microscopic observation showed abundant clusters of tangled mycelial filaments, Grocott+. At the edge, a nervous tissue was observed remodeled by an abundant inflammatory infiltrate consisting essentially of small lymphocytes and plasma cells. Additional immunohistochemistry was performed using anti-CD20, anti-CD3, anti-CD5, anti-CD23, anti-Bcl2, anti-Ki67, anti-IgD and anti-Kappa and Lamda antibodies. All taken together there were no further founding in support of a secondary localization of CLL. Cerebral aspergillosis is quite rare and often occurs in a context of immunosuppression. This case indicates the importance of a close collaboration between clinicians and pathologists for optimal patient care.

关键词： Cerebral Aspergillosis CLL Grocott Gomori Immunohistochemistry

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HPV/HBV or HPV/HCV Co-Infections in Women Treated for Chronic Hepatitis at Hôpital Saint Camille in Ouagadougou, Burkina Faso

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American Journal of molecular Biology（美国分子生物学期刊） 2024年第1期

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Detection of Human Papillomavirus DNA and E6/E7 mRNA from HPV Genotypes 16, 18, 31 and 33 in Histologically Confirmed Cases of Cervical Cancer and Precancerous Lesions in Burkina Faso

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American Journal of molecular Biology 2023年第4期13卷 276-290页

作者： Théodora mahoukèdè Zohoncon Shoukrat Ohuwa Toyin Bello Prosper Bado Rogomenoma Alice Ouédraogo Estelle Ouédraogo Ina marie Angèle Traoré Abdoul Karim Ouattara Florencia Wenkunni Djigma Albert Théophane Yonli Assita Sanou-Lamien olga mélanie lompo Jacques Simpore Laboratoire de Biologie moléculaire et de Génétique (LABIOGENE) Université Joseph KI-ZERBO Ouagadougou Burkina Faso Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA) Ouagadougou Burkina Faso Faculté des Sciences de la Santé Université Saint Thomas d’Aquin (USTA) Ouagadougou Burkina Faso Centre Hospitalo-Universitaire Yalgado Ouedraogo Ouagadougou Burkina Faso Pathological Anatomy and Cytology Laboratory Université Joseph Ki-Zerbo Ouagadougou Burkina Faso

Introduction: Cervical cancer, caused by persistent high-risk human papillomavirus (HPV) infection, remains a global public health problem. The cellular transformation and maintenance of the malignant phenotype of these HPVs are attributed to the viral oncoproteins E6 and E7. Objective: This study aims to detect the presence of human papillomavirus DNA and E6/E7 oncoprotein mRNA of HPV genotypes 16, 18, 31 and 33 in cases of cervical cancer and precancerous lesions, histologically confirmed in Burkina Faso. methods: This descriptive cross-sectional study focused on cases of cervical cancer and high-grade intraepithelial neoplasia (CIN) and was conducted from June to December 2022. One hundred (100) samples of fixed and paraffin-embedded tissues were collected from the pathological anatomy and cytology laboratories of hospitals in the capital of Burkina Faso. High-risk human papillomavirus (HR-HPV) DNA was detected using multiplex real-time PCR, while the presence of E6 and E7 mRNA in cervical cancer and high-grade CIN samples was determined using real-time Reverse Transcriptase-PCR (RT-PCR) with Taqman probes. Results: The mean age of women diagnosed with cervical cancer and high-grade CIN was 50.81 ± 13.65 years, ranging from 22 to 82 years. Cervical cancer and high-grade CIN were positive for at least one high-risk human papillomavirus (HR-HPV) in 80% of cases. The most prevalent genotypes observed were HPV16, 18, 31, and 33, collectively accounting for 70.08% of cases. Of the 89 samples that tested positive for HR-HPV genotypes 16, 18, 31, and 33, 88 (98.88%;95% CI: [94.58 - 99.94]) were also positive for the presence of mRNA encoding the E6 and E7 oncoproteins of HPV16, 18, 31, and 33. Conclusion: In the presence of HPV DNA, testing for E6 and E7 oncoprotein mRNA could serve as a promising biomarker and valuable tool for improved assessment of the progression to cervical cancer.

关键词： HPV E6/E7 Cervical Cancer Precancerous Lesions Burkina Faso

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Detection of Human Papillomavirus DNA and E6/E7 mRNA from HPV Genotypes 16, 18,31 and 33 in Histologically Confirmed Cases of Cervical Cancer and Precancerous Lesions in Burkina Faso

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American Journal of molecular Biology（美国分子生物学期刊） 2023年第4期

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molar Ectopic Pregnancy: A Case Study from the maternity Unit of the Bobo-Dioulasso Teaching Hospital

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Open Journal of Obstetrics and Gynecology 2016年第13期6卷 846-850页

作者： Bambara moussa Diallo Abdoul Azize lompo olga Goumbri Gynécologue Bobo-Dioulasso Burkina Faso

The authors reported a case of molar ectopic pregnancy seen and managed at the maternity unit of the Bobo-Dioulasso Teaching Hospital. The frequency of the molar ectopic pregnancy is difficult to assess. Besides, the histopathological examination of surgical specimen of the salpingectomy is not usual in our setting. For the management, we realized a total left salpingectomy with a histopathological examination of surgical specimens in association with serum b HCG follow up. During post operations period, the patient was put on estro-progestin contraception for a year, a regular checkup of the biological marker of the molar pregnancy till negativation, a clinical, ultrasound and radiological checkup. No anomaly was noticed at the end of the follow up.

关键词： Ectopic Pregnancy molar Pregnancy Prognosis

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Gastric Adenocarcinoma Treatment in Africa: Surgery Alone or Perioperative Chemotherapy?

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Journal of Cancer Therapy 2017年第7期8卷 653-662页

作者： Nayi Zongo Bagué Abdoul Halim Ouangré Edgar Bagré Sidpawalmde Carine Zida maurice Boukoungou Gilbert Sanou Adama lompo olga mélanie Traoré Si Simon Ahmadou Dem General and Digestive Surgery Yalgado Ouédraogo University Hospital Ouagadougou Burkina Faso Oncology Institute Joliot Curie of Dakar Dakar Senegal General and Digestive Surgery Department University Hospital Center Blaise COMPAORE Ouagadougou Burkina Faso Pathological Anatomy and Forensic Unit Yalgado Ouédraogo University Hospital Ouagadougou Burkina Faso

Aim: Evaluate the impact of mAGIC trial on gastric adenocarcinoma’s management in Africa. method and methodology: It was about a review of literature on therapeutic aspects of gastric adenocarcinoma in the African area. We have taken a census of 21 articles including 2792 patients published between 1980 and 2013. We have distinguished articles published before 2006 (group 1) from those published after 2006 (group 2) to better understand therapeutic changes after that perioperative chemotherapy has become a standard in gastric adenocarcinoma’s management. Results: Surgery remains in Africa the first and practically the only treatment weapon in gastric adenocarcinoma: 46% to 92% people in the 1st group and 65% to 100% people in the 2nd group underwent surgical procedures. Perioperative chemotherapy takes longer to be part of therapeutic habits (0.18%). Factors related to patients such alteration of general state with a WHO performance status superior to 2 in 72% of cases, the lack of financial accessibility to anticancerous drugs explains partly the non-use of perioperative chemotherapy. This is also due to factors peculiar to our sanitation structures which don’t have enough cancer specialists. So we noticed that mAGIC trial is simply ignored in certain studies. The lack of adoption of perioperative chemotherapy explains with delayed diagnosis the low survival of patients in the African area. Conclusion: mAGIC trial practically has no effect on therapeutic behavior yet comparatively to gastric adenocarcinoma in Africa. The insurance particularly relies on surgery only until now. However, it might enable us to improve gastric adenocarcinoma’s survival rates.

关键词： Adenocarcinoma Stomach Surgery Perioperative Chemotherapy Africa

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Tuberculosis of the Uterine Cervix: About a Case and Literature Review

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Open Journal of Obstetrics and Gynecology 2016年第12期6卷 734-739页

作者： Bambara moussa Konségré Valentin Ouattara Adama Diallo Abdoul Aziz Zampaligré Idrissa lompo olga Goumbri Gynécologue Bobo-Dioulasso Burkina Faso

The authors report a case of tuberculosis of the uterine cervix observed at the maternity unit of the Souro Sanon Teaching Hospital (CHUSS) of Bobo-Dioulasso. This is a rare localization of genital tuberculosis and can take the appearance of a cervical cancer. Biopsy with histological study of the lesion confirms the diagnosis. The frequency of this pathology can reach up to 8%. The extension to the endometrium and/or tubes in a young woman may compromise future fertility. Early and well conducted TB treatment allows the ad integrum restitution cervix.

关键词： Tuberculosis Cervical Biopsy Genital Tuberculosis

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