检索结果-南通市图书馆

Fine-needle aspiration technique under endoscopic ultrasound guidance:A technical approach for RNA profiling of pancreatic neoplasms

引用

World Journal of Gastrointestinal Oncology 2024年第6期16卷 2663-2672页

作者： Sabina Sherafedinovna Seyfedinova olga Aleksandrovna Freylikhman Polina Sergeevna Sokolnikova Konstantin Aleksandrovich Samochernykh Anna Aleksandrovna Kostareva olga Viktorovna kalinina Evgeniy Gennadievich Solonitsyn Research Laboratory of Digestive Cancer Almazov Medical Research CentreSaint-Petersburg 197341Russia Research Laboratory of Molecular and Cellular Modeling and Gene Therapy Almazov Medical Research CentreSaint-Petersburg 197341Russia Head of Russian Neurosurgical Institute Named after Prof.A.L.Polenova Russian Neurosurgical Institute Named after Prof.A.L.PolenovaSaint-Petersburg 191014Russia Head of Institute of Molecular Biology and Genetics Almazov National Medical Research CentreSaint-Petersburg 197341Russia Research Laboratory of Autoimmune and Autoinflammatory Diseases Almazov National Medical Research CentreSaint-Petersburg 197341Russia

BACKGROUND Early diagnosis of pancreatic ductal adenocarcinoma(PDAC)has been a longstanding *** prognosis of patients with PDAC depends on the stage at *** is necessary to identify biomarkers for the detection and differentiation of pancreatic tumors and optimize PDAC sample preparation procedures for DNA and RNA *** molecular studies are done using paraffin-embedded blocks;however,the integrity of DNA and RNA is often compromised in this ***,RNA isolated from human pancreatic tissue samples is generally of low quality,in part,because of the high concentration of endogenous pancreatic RNAse activity *** To assess the potential of endoscopic ultrasound-guided fine-needle aspiration(EUS-FNA)to obtain specimens from pancreatic neoplasms for subsequent RNA molecular profiling,including next-generation sequencing(NGS).METHODS Thirty-four EUS-FNA samples were included in this study:PDAC(n=15),chronic pancreatitis(n=5),pancreatic cysts(n=14),mucinous cysts(mucinous cystic neoplasia/intraductal papillary mucinous neoplasia)n=7,serous cystic neoplasms n=5,and pseudocysts n=*** material consisted of cyst fluid and cyst wall samples obtained by through-the-needle biopsy(TTNB).Samples were stored at -80℃ until *** purity(A260/230,A260/280 ratios),concentration,and integrity(RIN)were ***-time polymerase chain reaction was conducted on all samples,and small RNA libraries were prepared from solid mass *** RNA was successfully extracted from 29/34(85%)EUS-FNA samples:100% pancreatic adenocarcinoma samples,100% chronic pancreatitis samples,70% pancreatic fluid cyst samples,and 50%TTNB *** relative expression of GAPDH and HPRT were obtained for all successfully extracted RNA samples(n=29)including lowquality RNA *** concentration and nonoptimal RIN values(no less than 3)of RNA extracted from EUS-FNA samples did not prevent NGS library *** suitability of cyst fluid samples

关键词： Endoscopic ultrasound-guided,fine-needle aspiration Pancreatic cancer Pancreatic cysts RNA extraction Through-the-needle biopsy Next-generation sequencing

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chronic hepatitis B in pregnant women: Current trends and approaches

引用

World Journal of Gastroenterology 2021年第23期27卷 3279-3289页

作者： Maria Belopolskaya Viktor Avrutin olga kalinina Alexander Dmitriev Denis Gusev Polyclinical Department Botkin's Infectious Disease HospitalSt-Petersburg 195067Russia Chronic Viral Infectious Disease Lab Institute of Experimental MedicineSt-Petersburg 197376Russia Institute for Systems Theory University of StuttgartStuttgart 70569Baden-WurttembergGermany Faculty of Biomedical Sciences Almazov National Medical Research CentreSt-Petersburg 197341Russia Department of Molecular Microbiology Institute of Experimental MedicineSt-Petersburg 197376Russia Botkin's Infectious Disease Hospital St-Petersburg 195067Russia

Chronic hepatitis B(CHB)is a significant public health problem *** aim of the present review is to summarize the actual trends in the management of CHB in pregnant *** prevalence of hepatitis B virus(HBV)infection in pregnant women is usually comparable to that in the general population in the corresponding geographic *** women have to be screened for hepatitis B surface antigen(HBsAg)during *** examinations of pregnant women with CHB may include maternal hepatitis B e antigen,HBV viral load,alanine aminotransferase level,and HBsAg *** management of pregnancy depends on the phase of the HBV infection,which has to be determined before *** women of childbearing age with CHB,antiviral therapy can pursue two main goals:Treatment of active CHB,and vertical transmission *** pregnancy,tenofovir is the drug of choice in both cases.A combination of hepatitis B immunoglobulin and vaccine against hepatitis B should be administered within the first 12 h to all infants born to mothers with *** such cases,there are no contraindications to breastfeeding.

关键词： Chronic hepatitis B Hepatitis B viral load Pregnancy Antiviral treatment Newborns Mother-to-child transmission

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Intestinal microbiome changes in an infant with right atrial isomerism and recurrent necrotizing enterocolitis:A case report and review of literature

引用

World Journal of Clinical Cases 2022年第29期10卷 10583-10599页

作者： Aleksandra Kaplina Ekaterina Zaikova Artem Ivanov Yulia Volkova Tatiana Alkhova Vladimir Nikiforov Alexander Latypov Marina Khavkina Tatiana Fedoseeva Tatiana Pervunina Yulia Skorobogatova Svetlana Volkova Vladimir Ulyantsev olga kalinina Stanislav Sitkin Natalia Petrova Research Laboratory of Physiology and Diseases of Newborns Almazov National Medical Research CentreSt.Petersburg 197341Russia Research Laboratory of Autoimmune and Autoinflammatory Diseases Almazov National Medical Research CentreSt.Petersburg 197341Russia Vladimir Ulyantsev International Laboratory of Computer TechnologiesITMO UniversitySt.Petersburg 197101Russia Department of Cardiovascular Surgery for Children Almazov National Medical Research CentreSt.Petersburg 197341Russia Department of Neonatal Physiology with an ICU Ward Almazov National Medical Research CentreSt.Petersburg 197341Russia Pediatric Cardiac Intensive Care Unit Almazov National Medical Research CentreSt.Petersburg 197341Russia Neonatal and Preterm Special Care Unit(2nd Stage Care) Almazov National Medical Research CentreSt.Petersburg 197341Russia Institute of Perinatology and Pediatrics Almazov National Medical Research CentreSt.Petersburg 197341Russia Express Laboratory of Perinatal Centre Almazov National Medical Research CentreSt.Petersburg 197341Russia Clinical Diagnostic Laboratory Almazov National Medical Research CentreSt.Petersburg 197341Russia Epigenetics and Metagenomics Group Institute of Perinatology and PediatricsAlmazov National Medical Research CentreSt.Petersburg 197341Russia Department of Internal Diseases Gastroenterology and DieteticsNorth-Western State Medical University named after I.I.MechnikovSt.Petersburg 191015Russia

BACKGROUND Necrotizing enterocolitis(NEC)is a multifactorial disease that predominantly affects premature *** dysbiosis plays a critical role in NEC pathogenesis in premature *** main risk factor for NEC in term infants is mesenteric hypoperfusion associated with ductaldependent congenital heart disease(CHD)that eventually leads to intestinal *** incidence of NEC in neonates with critical CHD is 6.8%-13%.However,the role of the intestinal microbiome in NEC pathogenesis in infants with ductal-dependent CHD remains *** SUMMARY A male term neonate with right atrial isomerism underwent modified Blalock-Taussig shunt placement on the 14^(th)day of life and had persistent mesenteric hypoperfusion after *** patient had episodes of NEC stageⅡA on the 1^(st)and 28^(th)days after cardiac *** microbial composition was analyzed before and after cardiac surgery by sequencing region V4 of the 16S rRNA *** surgery,species belonging to genera Veillonella and Clostridia and class Gammaproteobacteria were detected,Bifidobacteriaceae showed a low *** first NEC episode was associated with postoperative hemodynamic instability,intestinal ischemiareperfusion injury during cardiopulmonary bypass,and a high abundance of Clostridium paraputrificum(Clostridium sensu stricto I)(56.1%).Antibacterial therapy after the first NEC episode resulted in increased abundance of Gammaproteobacteria,decreased abundance of Firmicutes,and low alpha *** changes in the microbial composition promoted the growth of Clostridium sensu strictoⅠ(72.0%)before the second NEC *** A high abundance of Clostridium sensu strictoⅠand mesenteric hypoperfusion may have contributed to NEC in the present case.

关键词： Necrotizing enterocolitis Congenital heart disease Ivemark syndrome Heterotaxy Right atrial isomerism Intestinal microbiome Case report

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

国际贸易对国家创新能力的影响 ——以金砖国家为例

国际贸易对国家创新能力的影响 ——以金砖国家为例

引用

作者：奥格（kalinina olga）东北师范大学

学位级别：硕士

当今世界经济经历着巨大变革,以科学知识和新技术为主的技术创新成为世界经济发展的动力。通过创建有效的国家创新体系,对国家的创新活动进行激励和引导,可以实现国际经济地位的快速提升。目前,不同国家的国家创新体系具有较大的差异,... 详细信息

当今世界经济经历着巨大变革,以科学知识和新技术为主的技术创新成为世界经济发展的动力。通过创建有效的国家创新体系,对国家的创新活动进行激励和引导,可以实现国际经济地位的快速提升。目前,不同国家的国家创新体系具有较大的差异,国际贸易中的技术贸易是创新领域的国际合作的重要内容,有助于国际贸易对特定国际创新发展的推动作用。国家参与全球价值链分工直接影响经济增长和经济发展,有助于降低成本并提高市场竞争力。巴西、俄罗斯、印度、中国和南非组成的金砖国家,经过多年的经济发展,不仅作为生产国,而且作为巨大的消费市场,在世界经济舞台中表现出日益的重要性。本论文研究国际贸易对金砖国家创新能力的影响,以期为其他欠发达国家的创新和发展过程提供借鉴。首先,论文总结了国家创新体系的概念、创新在经济发展中的作用,并分析了国家创新体系的主要特征。其次,对国际贸易影响国家创新能力的理论机制进行了分析,尤其是参与全球价值链、技术外溢和技术贸易的影响。第三,论文进行了国际贸易的主要因素对金砖国家创新影响的统计分析,如高科技产品的进出口、研究与开发成本以及外国直接投资等因素。第四,使用五个国家2000-2018年的面板数据,使用混合OLS模型、固定效应模型和随机效应模型,分析国际贸易因素对金砖国家创新发展的影响程度。研究表明,最主要的影响因素是高科技产品的出口以及政府在研发方面的支出。最后,针对实证研究的结果,提出了建立和发展国家创新能力的对策建议。

关键词：金砖国家国际贸易国家创新能力全球价值

来源：

同方学位论文库评论

在线全文

同方学位论文库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：