检索结果-南通市图书馆

Mobola plum seed methanolic extracts exhibit mixed type inhibition of angiotensinⅠ-converting enzyme in vitro

Asian Pacific Journal of Tropical Biomedicine 2017年第12期7卷 1079-1084页

作者： Olamide Olajusi Crown olanrewaju sam olayeriju Ayodele Oluwaseyi Kolawole Afolabi Clement Akinmoladun Mary Tolulope Olaleye Afolabi Akintunde Akindahunsi Department of Biochemistry the Federal University of TechnologyAkureNigeria

Objective: To explore the possible inhibitory potentials and mechanism by Mobola plum(Parinari curatellifolia) seeds crude methanol(CE) and flavonoid-rich(FE) extracts on angiotensin-1-converting enzyme(ACE Ⅰ).Methods: The sensitivity and kinetic model of inhibition of CE and FE on ACE Ⅰ using N-[3-(2-furyl)-acryloyl]-Phe-Gly-Gly as enzyme substrate for ACE Ⅰ was evaluated by Michealis Menten approach. The inhibition mechanism was explored from Lineweaver–Burk model and IC_(50) was determined from Cheng–Prusoff empirical ***: The IC_(50) of CE and FE were 13.54 and 39.38 mg/m L, respectively. Both extracts exhibited mixed type inhibition with the inhibitory constant(K_i) of CE was between0.38 and 0.37 mg/m L while that of FE showed a two-fold increase(1.62 mg/mL and0.28 mg/mL). FE on ACE Ⅰ demonstrated positive cooperativity with a Hill's coefficient ***: The study reveals the superior ACE Ⅰ inhibitory potential of CE over FE and suggest that mixed inhibition pattern of the enzyme might be the underlying mechanism of antihypertensive activity.

关键词： Angiotensin converting enzyme Blood pressure FAPGG Mixed type inhibition Parinari curatellifolia

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Ethylacetate extract of red onion(Allium cepa L.)tunic affects hemodynamic parameters in rats

引用

Food Science and Human Wellness 2015年第3期4卷 115-122页

作者： olanrewaju sam olayeriju Mary Tolulope Olaleye Olamide Olajusi Crown Kayode Komolafe Aline Augusti Boligon Margareth Linde Athayde Akintunde Afolabi Akindahunsi Department of Biochemistry School of SciencesThe Federal University of TechnologyPMB 704AkureNigeria Department of Biochemistry Faculty of ScienceFederal University Oye-EkitiPMB 373Oye-EkitiNigeria Department of Industrial Pharmacy Federal University of Santa Maria97105-900 Santa MariaRSBrazil

The effects of ethylacetate extract of red onion(Allium cepa)tunic(ACTE)on some hemodynamic and biochemical parameters were evaluated in normotensive albino *** pressure parameters were determined in anaesthetized rats orally administered ACTE(10-,20-,or 40 mg/kg)or ramipril(1 mg/kg)once daily for 14 ***,10-,20-,or 40 mg/kg ACTE produced significant(P<0.01),dose-dependent fall in systolic blood pressure,SBP(21%,27%,33%),diastolic blood pressure,DBP(6%,10%,16%),pulse pressure,PP(42%,49%,56%),mean arterial blood pressure,MAPB(13%,18%,23%)and heart rate,HR(4%,5%,7%).The highest effective dose(40 mg/kg)compared well with ramipril(1 mg/kg)with regards to SBP(41%),DBP(19%),PP(70%),MABP(29%)and HR(10%).Similar trends(decreases)were recorded for 40 mg/kg ACTE and ramipril,respectively,as regards the activities of serum enzymes:creatine kinase(60%and 65%),ALT(18%and 14%)and ALP(28%and 16%).HPLC fingerprints of the flavonoid-rich ACTE revealed that flavonols:quercetin,quercitrin,isoquercitrin,rutin and kaempferol are the active *** results demonstrate the hypotensive effect of *** tunic flavonoids initiating further investigation of their individual or synergistic contribution(s)to the observed effects.

关键词： acetate Blood rats

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：