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Identification of genes involved in the formation of soluble dietary fiber in winter rye grain and their expression in cultivars with different viscosities of wholemeal water extract

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The Crop Journal 2022年第2期10卷 532-549页

作者： Liudmila v.Kozlova Alsu R.Nazipova Oleg v.Gorshkov Liliya F.Gilmullina Olga v.Sautkina Natalia v.Petrova oksana I.Trofimova Sergey N.Ponomarev Mira L Ponomareva Tatyana A.Gorshkova Kazan Institute of Biochemistry and Biophysics Federal Research CenterKazan Scientific Center of the Russian Academy of Sciences420111 KazanRussia Tatar Scientific Research Institute of Agriculture Federal Research CenterKazan Scientific Center of the Russian Academy of Sciences420059 KazanRussia

The grain of rye(Secale cereale L.) used for baking contains a large amount of non-starch polysaccharides,making it an excellent component of functional foods. But rye grain intended for alcohol production and forage use should have a reduced content of these polysaccharides. A comprehensive parameter that can predict the best field of application for winter rye grain is the viscosity of its wholemeal water ***, our understanding of the genetic background underlying this key trait and associated features of rye grain is poor. By analyzing six Russian winter rye cultivars, we identified the most contrasting forms and characterized the peculiarities of their water-soluble carbohydrates capable of influencing the viscosity of water extracts. Then, using phylogenetic and transcriptomic analyses, we identified in the rye genome many genes encoding putative glycosyltransferases and glycosylhydrolases responsible for the synthesis and degradation of arabinoxylans, mixed-linkage glucans, cellulose, and some other polysaccharides. We determined the dynamics of m RNA abundance for these genes at three stages of kernel development. Comparisons of gene expression levels in two contrasting cultivars revealed specific members of multigene families that may serve as promising targets for manipulating non-starch polysaccharide content in rye grain. High-viscosity cultivars were characterized by up-regulation of many glycosyltransferases involved in the biosynthesis of arabinoxylans and other cell-wall polysaccharides,whereas low-viscosity cultivars showed up-regulation of several genes encoding polysaccharidedegrading enzymes.

关键词： Rye(Secale cereale) Kernel development Arabinoxylan Mixed-linkage glucan viscosity

Southern East Siberia Pliocene-Quaternary faults:Database,analysis and inference

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Geoscience Frontiers 2014年第4期5卷 605-619页

作者： oksana v.Lunina Riccardo Caputo Anton A.Gladkov Andrey S.Gladkov Institute of the Earth's Crust Siberian Branch of Russian Academy of Sciences University of Ferrara Department of Physics and Earth SciencesVia Saragat 144122 FerraraItaly

This paper presents the first release of an Informational System（IS）devoted to the systematic collection of all available data relating to Pliocene-Quaternary faults in southern East Siberia,their critical analysis and their seismotectonic *** final goal of this project is to form a new base for improving the assessment of seismic hazard and other natural processes associated with crustal *** presented IS has been exploited to create a relational database of active and conditionally active faults in southern East Siberia（between 100°-114° E and 50°-57° N）whose central sector is characterized by the highly seismic Baikal rift *** information within the database for each fault segment is organized as distinct but intercorrelated sections（tables,texts and pictures,etc.）and can be easily visualized as HTML pages in offline *** preliminary version of the database distributed free on disk already highlights the general fault pattern showing that the Holocene and historical activity is quite uniform and dominated by NE-SW and nearly E-W trending faults;the former with a prevailing dip-slip normal kinematics,while the latter structures are left-lateral strike-slip and oblique-slip（with different proportion of left-lateral and normal fault slip components）.These faults are mainly concentrated along the borders of the rift basins and are the main sources of moderate-to-strong（M≥5.5）earthquakes on the southern sectors of East Siberia in recent *** a whole,based on analyzing the diverse fault kinematics and their variable spatial distribution with respect to the overall pattern of the tectonic structures formed and/or activated during the late Pliocene-Quaternary,we conclude they were generated under a regional stress field mainly characterized by a relatively uniform NW-SE tension,but strongly influenced by the irregular hard boundary of the old Siberian *** obtained inferences are in an agreement with the existing models

关键词： Pliocene-Quaternary faults Database Seismic hazard Tectonics Southern East Siberia

Climate shapes the spatiotemporal variation in color morph diversity and composition across the distribution range of Chrysomela lapponica leaf beetle

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Insect Science 2022年第3期29卷 942-955页

作者： Mikhail v.Kozlov Zowi Oudendijk Anders Forsman vojtech Lanta Maxwell v.L.Barclay vladimir I.Gusarov Bert Gustafsson Zheng-Zhong Huang oksana Y.Kruglova Yuri M.Marusik Yuri E.Mikhailov Marko Mutanen Alexander Schneider Lukas Sekerka Maksim E.Sergeev vitali Zverev Elena L.Zvereva Department of Biology University of TurkuTurkuFinland Department of Animal Ecology and Physiology Radboud UniversityNijmegenThe Netherlands Department of Biology and Environmental Science Linnaeus UniversityKalmarSweden Institute of Botany The Czech Academy of SciencesDukelskaTrebohCzech Republic Department of Life Sciences Natural History MuseumLondonUnited Kingdom Natural History Museum University of OsloOsloNorway Departmant of Zoology Swedish Museum of Natural HistoryStockholmSweden elnsititute of Zoology Chinese Academy of SciencesBeijingChina Faculty of Biology Belarusian State UniversityMinskBelarus Department of Biocenology Institute for Biological Problems of the NorthFar East Branch of the Russian Academy of SciencesMagadanRussia Department of Zoology&Entomology University of the Free StateBloemfonteinSouth Africa Department of Ecology&Nature Management Ural State Forest Engineering UniversityYekaterinburgRussia Ecology and Genetics Research Unit University of OuluOuluFinland Department of Terrestrial Zoology Senckenberg Research Institute and Natural History MuseumFrankfurt am MainGermany Department of Entomology National MuseumPrague 9CirkusovaCzech Republic Federal Scientific Center of the East Asia Terrestrial Biodiversity Far East Branch of the Russian Academy of SciencesVladivostokRussia

Color polymorphism offers rich opportunities for studying the eco-evolutionary mechanisms that drive the adaptations of local populations to heterogeneous and changing *** explored the color morph diversity and composition in a Chrysomela lapponica leaf beetle across its entire distribution range to test the hypothesis that environmental and climatic variables shape spatiotemporal variation in the phenotypic structure of a polymorphic *** obtained information on 13617 specimens of this beetle from museums,private collections,and *** specimens(collected from 1830-2020)originated from 959 localities spanning 33°latitude,178°longitude,and 4200 m *** classified the beetles into five color morphs and searched for environmental factors that could explain the variation in the level of polymorphism(quantified by the Shannon diversity index)and in the relative frequencies of individual color *** highest level of polymorphism was found at high latitudes and *** color morphs differed in their climatic requirements;composition of colour morphs was independent of the geographic distance that separated populations but changed with collection year,longitude,mean July temperature and betweenyear temperature *** proportion of melanic beetles,in line with the thermal melanism hypothesis,increased with increasing latitude and altitude and decreased with increasing climate *** morph frequencies also declined during the past century,but only at high latitudes and altitudes where recent climate warming was especially *** observed patterns suggest that color polymorphism is especially advantageous for populations inhabiting unpredictable environments,presumably due to the different climatic requirements of coexisting color morphs.

关键词： adaptive polymorphism color morphs evolutionary dynamics geographic variation phenotypic diversity thermal melanism

European Court of Human Rights as a Guarantee of Observation the Medical Secrecy

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Journal of Forensic Science and Medicine 2021年第4期7卷 145-151页

作者： Olena v Prudnykova vasil M Pyvovarov Olena v Fedosova oksana A Stasevska Olga v Umanets department of Culturology Yaroslav Mudryi National Law UniversityKharkivUkraine Research Laboratory for the Problems on Combating Crime Kharkiv National University of Internal AffairsKharkivUkraine

The European Convention on Human Rights proclaims the right of everyone to privacy of information about their state of health,which is a particularly important issue during the COvID-19 *** aim of the study is to determine the essence of the ECtHR's activity as a guarantee of information culture,highlight the main elements of its activities in this aspect,and analyze the right to medical secrecy in the practice of the European Court of Human *** formal-logical method was used to analyze the norms of the European Convention on Human Rights,determine the content of basic concqts,and systematize the material in order to obtain generalizing conclusions within the framework of the stated *** predictive method was used to determine the prospects for the development of legislation aimed at creating a system of effective legal provision for the confidentiality of patient *** European Court of Human Rights has more than once drew attention to the enormous importance of protecting personal,in particular medical data,so that a person can enjoy his or her right to private and family life with *** is why public authorities must ensure the confidentiality of information about the health of *** should not be disclosed or processed without the prior informed consent of the person *** are a lot of cases of unlawful disclosure of information about the patienfs health and liability for unlawful disclosure.

关键词： Confidentiality European convention on human rights illegal disclosure of information medical secrecy respect for private life

Euxinia as a dominant process during OAEla(Early Aptian)on the Eastern Russian Platform and during OAElb(Early Albian)in the Middle Caspian

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Science China Earth Sciences 2017年第1期60卷 58-70页

作者： Svetlana O. ZORINA oksana v. PAvLOvA Bulat M. GALIULL1N vladimir P. MOROZOv Alexey A. ESKIN Institute of Geology and Petroleum Technologies Kazan Federal University 4/5 Kremlyovskaya street Kazan 420008 Russian Federation

A detailed study of the mineral composition and microstructure of the black shales associated with OAEla(Eastern Russian Platform),OAElb(Middle Caspian),and the host rocks has been carried out using X-ray diffraction,scanning electron microscopic,and microprobe *** results provide important constraints for depositional environments in the sedimentary *** shales with pyrite framboids imply euxinic(sulfidic)conditions with increased organic matter *** framboids suggest partial or complete dissolution of the organic matter inside the framboids due to increasing water *** on the Eastern Russian Platform is heterogeneous as it includes thin interbeds of concretionary coccolith limestones within the interval of bituminous shales,and correlates with the Lower Aptian Rhagodiscus angustus nannofossil *** coccolith limestones indicate short intermittent episodes of interrupted stagnation,rapid oxygenation,and restoration of normal marine *** presence of montmorillonite,albite,microcline,and diopside in the bituminous siltstones and in the host siltstones of OAEla on the Eastern Russian Platform,as well as a high content of titanium in the black siltstones correlated with OAElb in the Middle Caspian allow proposing significant input of pyroclastic material into the extant sea.

关键词： OAE1a OAE1b Black shales Pyrite framboids Euxinia Oxygenation Eastern Russian Platform Middle Caspian Cretaceous

The Usefulness of Zhukovsky Double Balloon in Obstetric Hemorrhage

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Maternal-Fetal Medicine 2019年第1期1卷 10-17页

作者： Sergey v.Barinov Irina v.Medyannikova Anna v.Borisova Yulia I.Tyrskaya Irina v.Savelieva Inna v.Shamina oksana v.lazarev. Tatyana v.Kadtsyna The Second Department of Obstetrics and Gynaecology Federal State Budget Institution of HigherProfessional EducationOmsk State Medical Universitythe Russian Ministry of HealthcareOmsk644043Russia Department of Obstetrics and Gynaecology Federal State Budget Institution ofHigher EducationPeoples'Friendship Universitythe Russian Ministry of HealthcareMoscow117198Russia

Objective:To evaluate the effectiveness of the use of a modified Zhukovsky double(vaginal and uterine)balloon to improve the results of treatment in women with obstetric ***:We conducted an observational controlled study including 701 puerperas,which were divided into two groups:The main group consisted of 508 women,who underwent a combined management,that is,traditional(transfusion of fresh frozen plasma,erythrocyte mass,uterotonics,hemostatic agents),surgical hemostasis in cases of caesarean section,and insertion of a double Zhukovsky balloon;while the comparison group included 193 patients,who were managed *** main group and the comparison group were divided into subgroups according to the main etiology of obstetric hemorrhage:ⅠA andⅠB-postpartum;ⅡA andⅡB-placenta accreta;ⅢA andⅢB-postpartum ***:The most frequent causes of massive obstetric hemorrhage were atony of the uterus(39.2%),placenta previa(29.1%),and placental abruption(11.5%).Of the 508 balloon insertions,345(70.7%)were inserted for hemorrhage during cesarean section and 148(29.3%)for obstetric hemorrhage after spontaneous *** the obstetric hemorrhage at caesarean section,lower segment bleeding prevailed(78.1%),caused mainly by placenta previa,placenta accreta,placental abruption,polyhydramnios,and uterine scar *** management,including surgical hemostasis and insertion of vaginal and uterine balloon of Zhukovsky,reduced blood loss by 1.5 times and the number of hysterectomies by 6.72 times compared to ***:The use of a modified Zhukovsky double balloon in the management of obstetric hemorrhage may reduce the number of hysterectomies and the amount of blood loss.

关键词： Obstetric hemorrhage Placenta previa Accrete vaginal wall rupture Uterine ligation Hypotonic hemorrhage

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淡水蜗牛视觉系统的输入与输出通路(英文)

Zoological Research 2011年第4期32卷 403-420页

作者： oksana P Tuchina valery v Zhukov victor Benno Meyer-Rochow School of Engineering and Science Jacobs UniversityBremen D-28759Germany Department of Agricultural and Soil Ecology Faculty of Bioresources and Natural UsageKaliningrad State Technical UniversityKaliningrad 236000Russia

通过神经生物素在视神经上的逆行性传输对淡水蜗牛(Planorbarius corneus)视网膜及中央神经节的输入、输出神经元进行标记。由于没有发现突触联系,所以至少一部分光感受细胞的轴突可被视为直接参与形成视神经。这些神经元的轴突进入大... 详细信息

通过神经生物素在视神经上的逆行性传输对淡水蜗牛(Planorbarius corneus)视网膜及中央神经节的输入、输出神经元进行标记。由于没有发现突触联系,所以至少一部分光感受细胞的轴突可被视为直接参与形成视神经。这些神经元的轴突进入大脑神经节形成密集的细传入神经纤维束-视神经堆。传出神经元则存在于除颊部以外的所有神经节。一些上行轴突在大脑神经节处分叉,通过脑-脑联合,到达对侧眼并在眼杯处形成分枝。部分传出神经元的轴突也投射于不同的外周神经,如:***,pallialis dexter,pallialis sinister internus et externus。五羟色胺能纤维和FMRF-酰胺能纤维均存在于视神经上,且这些纤维隶属于只投射在同侧眼的中央神经元。它们形成了位于眼杯处的丰富曲张结构及视网膜核心层,并且可能有助于调节视网膜对光的敏感性。

关键词：腹足纲神经系统眼逆行性传输五羟色胺 FMRF-酰胺

Expression of auxin synthesis gene tms1 under control of tuber-specific promoter enhances potato tuberization in vitro

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Journal of Integrative Plant Biology 2015年第9期57卷 734-744页

作者： oksana O.Kolachevskaya valeriya v.Alekseeva Lidiya I.Sergeeva Elena B.Rukavtsova Irina A.Getman Dick vreugdenhil Yaroslav I.Buryanov Georgy A.Romanov Signaling Systems Laboratory Timiryazev Institute of Plant Physiology Russian Academy of Sciences Plant Biotechnology Laboratory Branch of Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences Laboratory of Plant Physiology Wageningen University Department of Molecular Basis of Ontogenesis Belozersky Institute of Physico-Chemical Biology Moscow State University

Phytohormones, auxins in particular, play an important role in plant development and productivity. Earlier data showed positive impact of exogenous auxin on potato (Solanum tuberosum L.) tuberization. The aim of this study was to generate potato plants with increased auxin level predominantly in tubers. To this end, a pBinB33-tms1 vector was constructed harboring the Agrobacterium auxin biosynthesis gene tms1 fused to tuber-specific promoter of the class I patatin gene (B33-promoter) of potato. Among numerous independently generated B33:tms1 lines, those without visible differences from control were selected for detailed studies. In the majority of transgenic lines, tms1 gene transcription was detected, mostly in tubers rather than in shoots. Indoleacetic acid (IAA) content in tubers and the auxin tuber-to-shoot ratio were increased in tms1-expressing transformants. The organ-specific increase in auxin synthesis in B33:tms1-transformants accelerated and intensified the process of tuber formation, reduced the dose of carbohydrate supply required for in vitro tuberization, and decreased the photoperiodic dependence of tuber initiation. Overall, a positive correlation was observed between tms1 expression, IAA content in tubers, and stimulation of tuber formation. The revealed properties of B33:tms1 transformants imply an important role for auxin in potato tuberization and offer prospects to magnify potato productivity by a moderate organ-specific enhancement of auxin content.

关键词： Auxin gene expression Solanum tuberosum tms1 transgenic potato tuberization tuber yield

Distribution of serotonin and FMRF-amide in the brain of Lymnaea stagnalis with respect to the visual system

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Zoological Research 2012年第3期33卷 1-12页

作者： oksana P.TUCHINA valery v.ZHUKOv victor B.MEYER-ROCHOW School of Engineering and Science Jacobs UniversityBremen D-28759Germany Department of Agricultural and Soil Ecology Faculty of Bioresources and Natural UsageKaliningrad State Technical UniversityKaliningrad 236000Russia Department of Biology Oulu UniversityOuluSF 90300 Finland

Despite serotonin’s and FMRF-amide’s wide distribution in the nervous system of invertebrates and their importance as neurotransmitters,the exact roles they play in neuronal networks leaves many *** mapped the presence of serotonin and FMRF-amide-immunoreactivity in the central nervous system and eyes of the pond snail Lymnaea stagnalis and interpreted the results in connection with our earlier findings on the central projections of different peripheral *** the chemical nature of the intercellular connections in the retina of *** is still largely unknown,we paid special attention to clarifying the role of serotonin and FMRF-amide in the visual system of this snail and compared our findings with those reported from other *** least one serotonin-and one FMRF-amidergic fibre were labeled in each optic nerve,and since no cell bodies in the eye showed immunoreactivity to these neurotransmitters,we believe that efferent fibres with somata located in the central ganglia branch at the base of the eye and probably release 5HT and FMRF-amide as *** labelling revealed retrograde transport of neurobiotin through the optic nerve,allowing us to conclude that the central pathways and serotonin-and FMRF-amide-immunoreactive cells and fibres have different locations in the CNS in *** chemical nature of the fibres,which connect the two eyes in ***,is neither serotoninergic nor FMRF-amidergic.

关键词： Gastropoda Nervous system Retina visual projections Retrograde transport Immunocytochemistry Neurotransmitters