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Effect of Hypoxia on the Expression of a Subset of Proliferation Related Genes in IRE1 Knockdown U87 Glioma Cells

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Advances in Biological Chemistry 2017年第6期7卷 195-210页

作者： Dariia O. Tsymbal Dmytro O. Minchenko oksana S. Hnatiuk Olha Y. Luzina Oleksandr H. Minchenko Department of Molecular Biology Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine Kyiv Ukraine

We have studied the expression of a subset of genes encoding important tumor growth related factors in U87 glioma cells with IRE1 (inositol requiring enzyme-1) knockdown as well as their hypoxic regulation. It was shown that the expression levels of activating transcription factor 6 (ATF6), clusterin (CLU), adhesion G protein-coupled receptor E5 (ADGRE5), transglutaminase?2, C polypeptide (TGM2), leukemia inhibitory factor (LIF), phosphoserine aminotransferase 1 (PSAT1), glyoxalase I (GLO1) and tetraspanin 13 (TSPAN13) are significantly down-regulated in glioma cells with the knockdown of IRE1 signaling enzyme. It was also shown that in glioma cells subjected to hypoxia, the expression levels of PSAT1, TSPAN13, EIF2AK3, and TGM2 genes were up-regulated, whereas the expression of ATF6 gene was down-regulated. At the same time, the expression levels of LIF, CLU, and ADGRE5 genes did not change in response to hypoxic treatment.?Furthermore, inhibition of IRE1, a key effector of an unfolded protein response pathway, modified the effect of hypoxia on the expression of most studied genes. Present study demonstrates that IRE1 knockdown down-regulated the expression of most studied genes and modified their hypoxic regulation and that these changes possibly contributed to the suppression of glioma growth in cells without IRE1 signaling enzyme function.

关键词： IRE1 Knockdown Hypoxia mRNA Expression EIF2AK3 ATF6 CLU PSAT1 TGM2 U87 Glioma Cells

The vascular endothelial growth factor genes expression in glioma U87 cells is dependent from ERN1 signaling enzyme function

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Advances in Biological Chemistry 2012年第2期2卷 198-206页

作者： Dmytro O. Minchenko Kateryna I. Kubaichuk oksana O. Ratushna Serhiy V. Komisarenko Oleksandr H. Minchenko Department of Molecular Biology Palladin Institute of Biochemistry National Academy of Sciences of Ukraine Kiev Ukraine

The expression of different vascular endothelial growth factor (VEGF) genes was studied in glioma U87 cells with endoplasmic reticulum–nuclei-1 (ERN1) loss of function and its regulation by hypoxia and glutamine or glucose deprivation conditions as model of ischemia. The blockade of function of the ERN1 enzyme, which is a major sensor of endoplasmic reticulum stress, leads to a decrease of the VEGFA, VEGFB and VEGFC mRNA expression level. The level of VEGFA proteins also decreases at this experimental condition in the cytosolic fraction, but increases in the nuclear fraction. Hypoxia does not affect VEGFC and increases the expression level of VEGFA and VEGFB mRNA in both used cell types, however, the change was much less profound in cells with suppressed function of ERN1. The expression level of VEGFC mRNA decreases in both used cell types in glutamine deprivation condition, however, the change was more profound in control glioma cells. At the same time, the expression level of VEGFA mRNA increases and VEGFB—decreases in gluta-mine deprivation condition in control glioma cells only. Exposure of glioma cells to glucose deprivation condition increases VEGFB mRNA expression level in both used cell types;however, VEGFA—in control glioma cells only and VEGFC—in cells with ERN1 signaling enzyme loss of function only. Thus, the results of this study clearly demonstrated the down-regulation of the expression of all three VEGF genes in glioma cells with ERN1 loss of function which correlates to the suppressed angiogenesis and proliferation rate of these cells. Moreover, the effect of hy-poxia and glutamine or glucose deprivation condition on the expression level of all VEGF genes is different and mainly depends on ERN1 signaling enzyme function.

关键词： VEGF Expression ERN1 Hypoxia Ischemic Conditions Angiogenesis Glioma U87 Cells

Climate shapes the spatiotemporal variation in color morph diversity and composition across the distribution range of Chrysomela lapponica leaf beetle

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Insect Science 2022年第3期29卷 942-955页

作者： Mikhail V.Kozlov Zowi Oudendijk Anders Forsman Vojtech Lanta Maxwell V.L.Barclay Vladimir I.Gusarov Bert Gustafsson Zheng-Zhong Huang oksana Y.Kruglova Yuri M.Marusik Yuri E.Mikhailov Marko Mutanen Alexander Schneider Lukas Sekerka Maksim E.Sergeev Vitali Zverev Elena L.Zvereva Department of Biology University of TurkuTurkuFinland Department of Animal Ecology and Physiology Radboud UniversityNijmegenThe Netherlands Department of Biology and Environmental Science Linnaeus UniversityKalmarSweden Institute of Botany The Czech Academy of SciencesDukelskaTrebohCzech Republic Department of Life Sciences Natural History MuseumLondonUnited Kingdom Natural History Museum University of OsloOsloNorway Departmant of Zoology Swedish Museum of Natural HistoryStockholmSweden elnsititute of Zoology Chinese Academy of SciencesBeijingChina Faculty of Biology Belarusian State UniversityMinskBelarus Department of Biocenology Institute for Biological Problems of the NorthFar East Branch of the Russian Academy of SciencesMagadanRussia Department of Zoology&Entomology University of the Free StateBloemfonteinSouth Africa Department of Ecology&Nature Management Ural State Forest Engineering UniversityYekaterinburgRussia Ecology and Genetics Research Unit University of OuluOuluFinland Department of Terrestrial Zoology Senckenberg Research Institute and Natural History MuseumFrankfurt am MainGermany Department of Entomology National MuseumPrague 9CirkusovaCzech Republic Federal Scientific Center of the East Asia Terrestrial Biodiversity Far East Branch of the Russian Academy of SciencesVladivostokRussia

Color polymorphism offers rich opportunities for studying the eco-evolutionary mechanisms that drive the adaptations of local populations to heterogeneous and changing *** explored the color morph diversity and composition in a Chrysomela lapponica leaf beetle across its entire distribution range to test the hypothesis that environmental and climatic variables shape spatiotemporal variation in the phenotypic structure of a polymorphic *** obtained information on 13617 specimens of this beetle from museums,private collections,and *** specimens(collected from 1830-2020)originated from 959 localities spanning 33°latitude,178°longitude,and 4200 m *** classified the beetles into five color morphs and searched for environmental factors that could explain the variation in the level of polymorphism(quantified by the Shannon diversity index)and in the relative frequencies of individual color *** highest level of polymorphism was found at high latitudes and *** color morphs differed in their climatic requirements;composition of colour morphs was independent of the geographic distance that separated populations but changed with collection year,longitude,mean July temperature and betweenyear temperature *** proportion of melanic beetles,in line with the thermal melanism hypothesis,increased with increasing latitude and altitude and decreased with increasing climate *** morph frequencies also declined during the past century,but only at high latitudes and altitudes where recent climate warming was especially *** observed patterns suggest that color polymorphism is especially advantageous for populations inhabiting unpredictable environments,presumably due to the different climatic requirements of coexisting color morphs.

关键词： adaptive polymorphism color morphs evolutionary dynamics geographic variation phenotypic diversity thermal melanism

X-Ray Diffractometry and Raman Spectroscopy Investigation of Irradiated Layered III-VI Crystals

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材料科学与工程（中英文A版） 2012年第7期2卷 537-543页

作者： Zakhar Dmytrovych Kovalyuk Oleg Mykolayovych Sydor oksana Anatoliivna Sydor Volodymyr Grygorovych Tkachenko Igor Mykolayovych Maksymchuk Volodymyr Ivanovych Dubinko Pavlo Mykolayovych Ostapchuk Chernivtsi Department of the Institute of Materials Science Problems Chernivtsi 58001 Ukraine Institute of Materials Science Problems Kyiv 03680 Ukraine National Scientific Center "Kharkiv Physico-Technical Institute " Kharkiv 61108 Ukraine

The present results of investigations of the influence of irradiation on layered n-InSe, p-InSe, and p-GaSe semiconductors. Two types of irradiation are considered: (1) the bremsstrahlung ?-quanta with effective energy of 3MeV and the irradiation dose ranging from 0.14 to 140 kGy and (2) 12 MeV electron irradiation up to the dose ranging from 3.3 to 33 MGy. The crystal structure, lattice parameters, and atom coordination environment were investigated by means of X-ray structure analysis and Raman spectra measurements. It is shown that all samples under investigation have high structural perfection. It is shown that even the maximum dose levels of ?-quanta and electrons did not caused qualitative changes in the investigated spectra, which indicates that the influence of the irradiation on the subsystems of vacancies and impurities in InSe and GaSe is insignificant. Possible physical phenomena occurring in the layered crystals subjected to irradiation are described. It is suggested tentatively that the dopant atoms of Cd in p-InSe are the centers for localization of radiation-induced vacancies, whereas in the intentionally undoped crystals, the vacancies are localized in the sublattices of In or Ga. These results enable one to consider layered semiconductors as potential candidates for preparation of radiation-resistant photoconverters or detectors of high-energy particles.

关键词： X-射线衍射拉曼光谱电子辐照晶体结构照射剂量层状晶体晶格常数调查结果

Evaluation of Carbon and Electron Flow in Lactobacillus brevis as a Potential Host for Heterologous 1-Butanol Biosynthesis

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Advances in Microbiology 2013年第5期3卷 450-461页

作者： oksana V.Berezina German Jurgens Natalia V.Zakharova Rustem S.Shakulov Sergey V.Yarotsky Tom B.Granstrom State Research Institute of Genetics and Selection of Industrial Microorganisms MoscowRussian Federation Department of Biotechnology and Chemical Technology School of Chemical TechnologyAalto UniversityEspooFinland

Heterofermentative lactic acid bacterium Lactobacillus brevis may be considered as a promising host for heterologous butanol synthesis because of tolerance to butanol and ability to ferment pentose and hexose sugars from wood hydrolysates that are cheap and renewable carbohydrate source. Carbon and electron flow was evaluated in two L. brevis strains in order to assess metabolic potential of these bacteria for heterologous butanol synthesis. Conditions required for generation of acetyl-CoA and NADH which are necessary for butanol biosynthesis have been determined. Key enzymes controlling direction of metabolic fluxes in L. brevis in various redox conditions were defined. In anaerobic glucose fermentation, the carbon flow through acetyl-CoA is regulated by aldehyde dehydrogenase ALDH possessing low affinity to NADH and activity (KmNADH= 200 μM, Vmax= 0.03 U/mg of total cell protein). Aerobically, the NADH-oxidase NOX (KmNADH= 25 μM, Vmax = 1.7 U/mg) efficiently competes with ALDH for NADH that results in formation of acetate instead of acetyl-CoA. In general, external electron acceptors (oxygen, fructose) and pentoses decrease NADH availability for native ethanol and recombinant butanol enzymes and therefore reduce carbon flux through acetyl-CoA. Pyruvate metabolism was studied in order to reveal redirection possibilities of competitive carbon fluxes towards butanol synthesis. The study provides a basis for the rational development of L. brevis strains producing butanol from wood hydrolysate.

关键词： Lactobacillus brevis Metabolism Carbon and Electron Flow Heterologous Butanol Synthesis SO2-Ethanol-Water Hydrolysate

腺苷受体是调节大脑内侧前额叶皮质星形胶质细胞大麻素1型(CB1)受体对突触可塑性作用的开关

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神经损伤与功能重建 2024年第4期19卷 250-250页

作者： Joana Gon?alves-Ribeiro oksana K Savchak Sara Costa-Pinto Joana I Gomes Rafael Rivas-Santisteban Alejandro Lillo Javier Sánchez Romero Ana M Sebasti?o Marta Navarrete Gemma Navarro Rafael Franco Sandra H Vaz Instituto de Farmacologia e Neurociencias Faculdade de MedicinaUniversidade de Lisboa Instituto de Medicina Molecular Joao Lobo Antunes Faculdade de MedicinaUniversidade de Lisboa Department of Biochemistry and Physiology School of Pharmacy and Food ScienceUniversitat de Barcelona CiberNed Network Center for Neurodegenerative DiseasesNational Spanish Health Institute Carlos Ⅲ 5. Instituto CajalCSIC PhD Program in Neuroscience Universidad Autonoma de Madrid-Instituto Cajal Institut de Neurociencies Universitat de Barcelona Molecular Neurobiology Laboratory Department of Biochemistry and Molecular BiomedicineFaculty of Biology School of Chemistry Universitat de Barcelona

内侧前额叶皮质(mPFC)参与诸如工作记忆等认知功能。星形胶质细胞大麻素1型受体(CB1R)诱导细胞质内钙离子(Ca2+)浓度变化,对神经元功能产生影响。mPFC的星形胶质细胞也表达腺苷A1和A2A受体(A1R,A2AR),但目前尚不清楚这些细胞中的CB1R与... 详细信息

内侧前额叶皮质(mPFC)参与诸如工作记忆等认知功能。星形胶质细胞大麻素1型受体(CB1R)诱导细胞质内钙离子(Ca2+)浓度变化,对神经元功能产生影响。mPFC的星形胶质细胞也表达腺苷A1和A2A受体(A1R,A2AR),但目前尚不清楚这些细胞中的CB1R与腺苷受体之间的交流。我们在这里展示,通过CB1R-A2AR或CB1R-A1R异源二聚体,星形胶质细胞Ca2+信号传递发生了进一步的调节,最终影响mPFC突触可塑性。当A1R和A2AR被激活时,CB1R介导的Ca2+瞬变出现增加或减少,揭示了腺苷受体可能是星形胶质细胞CB1R的调节器。CB1R的激活导致mPFC的长时程增强(LTP)增强,受A1R控制,但不受A2AR控制。值得注意的是,在IP3R2KO小鼠中,由于没有显示出星形胶质细胞Ca2+水平升高,CB1R的激活减少了LTP,这种效果不受A1R或A2AR的调节。本研究表明,CB1R在mPFC LTP上具有稳态作用,受到A1R的控制,可能是由于这些受体在星形胶质细胞中的交流,最终改变了CB1R Ca2+信号传递。

关键词： CB1受体腺苷受体星形胶质细胞内侧前额叶皮质突触可塑性

Psychotropic effects of aspirin, acetylsalicylate cobalt and acetylsalicylate zinc at various doses

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Health 2012年第11期4卷 1041-1045页

作者： Tatyana V. Yakovchyuk oksana V. Katiushyna Ivan I. Koreniuk Denis R. Khusainov Tatyana V. Gamma Department of Human and Animal Physiology and Biophysics Faculty of Biology National Tavrida Vernadskii University Simfe-ropol Ukraine

For the first time it is shown that psychotropic action of acetylsalicylates at various doses is manifested as a nonmonotonic dependence having its peaks at therapeutic and ultra-low dose zones. It is discovered that development of effects of aspirin resembles that of acetylsalicylate zinc. Acetylsalicylate cobalt at extremely low doses zone showed the highest antidepressant activity, demonstrating toxicity at high doses. Generally, it is revealed that the use of aspirin and its salts at high doses range causes maximum psychotropic effects development, usually accompanied by side-effects. Therefore, aspirin, acetylsalicylate cobalt and zinc at extremely low doses are recommended for further study as psychotropic medications.

关键词： Acetylsalicylic Acid Acetylsalicylate Cobalt Acetylsalicylate Zinc Psychotropic Action Behavioral Tests Ultra-Low Doses Rats

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一对俄罗斯夫妇的爱情表达

档案春秋 2015年第12期 24-25页

作者：雨竹

通常,民众心理会认为,只有女性才会为了爱情做一些生活方面的调整。比如说,离开公司做家庭主妇,照顾家人,改变住址（包括到陌生的国家生活）,和家人;面对挑战,一起攻克难坚。俄罗斯民族在感情方面是比较专一的民族,人们认为事业可以变动... 详细信息

通常,民众心理会认为,只有女性才会为了爱情做一些生活方面的调整。比如说,离开公司做家庭主妇,照顾家人,改变住址（包括到陌生的国家生活）,和家人;面对挑战,一起攻克难坚。俄罗斯民族在感情方面是比较专一的民族,人们认为事业可以变动,环境可以改变,困难可以克服,但你的家一定要在身边。不只是俄

关键词：弗拉基米尔远东上海中华人民共和国俄罗斯

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“芭比娃娃”追梦路

档案春秋 2015年第9期 35-36页

作者：雨竹

印度伟达先知曾说:"没有什么对不对的路,顺其自然接受现有的内外生活。"自从2010年我从乌克兰的哈尔科夫来到中国的北京,继而来到上海之后,经过数年的适应和磨砺,现在,我毫无保留地喜欢上了这座城市。这里,改变了我的人生轨迹,记录了我... 详细信息

印度伟达先知曾说:"没有什么对不对的路,顺其自然接受现有的内外生活。"自从2010年我从乌克兰的哈尔科夫来到中国的北京,继而来到上海之后,经过数年的适应和磨砺,现在,我毫无保留地喜欢上了这座城市。这里,改变了我的人生轨迹,记录了我的梦想与生活。从语言适应、生活习惯、文化习俗等一个个难关中闯过来,内心一直被一种神奇的动力所牵引——这种力量来源于上海的无限机遇和欣欣向荣的时尚气息。

关键词：哈尔科夫乌克兰芭比娃娃现代舞上海瑜伽建筑设计公司中文