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Effect of Disulfiram/Copper Gluconate Combination on Haematological Indices in Rodents

Pharmacology & Pharmacy 2015年第1期6卷 17-24页

作者： Georgewill Udeme Owunari Siminialayi Iyeopu Minakiri obianime atuboyedia wolfe Department of Pharmacology Faculty of Basic Medical Sciences College of Health Sciences University of Port Harcourt Port Harcourt Nigeria

The chronic toxicological profile of disulfiram/copper gluconate (DSF/CG) combination was investigated in a 90 day time and dose dependent study. A total of 148 rats weighing 260 - 300 g were used for this study;60 for the pilot study and 88 for the chronic toxicity test. 88 rats divided into eleven groups consisting of 8 rats each were used for the main experiment. Groups 1 and 2 served as control groups and received normal saline as placebo and 99.5% dimethyl sulfoxide (DMSO) (Solvent control), respectively. Drugs were administered orally via a 1 ml syringe. Animals were given three doses (1/5th, 1/10th and 1/20th) of the calculated LD50 of 373 mg/kg and 75 mg/kg for disulfiram and copper gluconate respectively. Dosing was done daily with that of the combination given 12 hours apart. Blood samples were obtained via cardiac puncture on days 30, 45, 60 and 90 for analysis. Haematological parameters showed a significant (p < 0.05) dose- and time-dependent decrease in the packed cell volume, red blood cell count, white blood cell count and platelet count respectively. The results indicate bone marrow depression evidenced by anemia, leucopenia and thrombocytopenia in the experimental animals. The DSF/CG combination appears to exhibit a synergistic dose-dependent haematotoxicity.

关键词： Disulfiram Copper gluconate Haematological Indices

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Effects of aqueous extracts of Irvingia gabonensis seeds on the hormonal parameters of male guinea pigs

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Asian Pacific Journal of Tropical Medicine 2010年第3期3卷 200-204页

作者： obianime Acubowyedia wolfe Uche Fidelia Ijeoma Department of Pharmacology University of Port Harcourt Nigeria Department of Pharmacognosy and Phytotherapy University of Port Harcourt Nigeria

Objective:To investigate the effects of the aqueous extracts of Irvingia gabonensis(Irvingaceae) seeds on the hormonal parameters of male guinea ***:The hormonal effects of Irvingia gabonensis on hormonal parameters of male guinea pigs were investigated and compared with that of proviron using enzyme immuno assay method,which was done by reaction of antibody with serum testosterone and testosterone label,magnetic solid phase separation and colour development *** phytochemical screening of Irvingia gabonensis seeds was also carried out using standard ***:The aqueous extract of the Irvingia gabonensis seeds(50-400 mg/kg) caused a statistically significance increase(P<0.05 ANOVA) of testosterone in male guinea pigs,from (2.70±0.26) ng/mL to(3.10±0.42) ng/mL on the 7th day and to(3.30±0.48) ng/mL on the 28th day of the administration of the *** highest increase was(3.30±0.48) ng/mL,being obtained after 28 days of *** effects were similar to that of proviron,which was (2.80±0.28) ng/mL and(3.00±0.41) ng/mL on the 7th and 28th day of treatment respectively. The phytochemical screening of Irvingia gabonensis seeds revealed the presence of flavonoids. tannin,alkaloids,carbohydrate,volatile oils,terpenoids,saponins and cardiac glycosides. Conclusions:This study supports the claims on the use of the seeds of Irvingia gabonensis by traditional medicine practitioners as a fertility ***,further studies need to be done to isolate and characterize the active principle(s) responsible for this activity in this plant.

关键词： Irvingia gabonensis Hormonal parameters Male guinea pigs

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Toxicological Evaluation of Disulfiram, Copper Gluconate and Disulfiram/Copper Gluconate Combination on Renal Function in Rodents

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Pharmacology & Pharmacy 2015年第2期6卷 86-93页

作者： Udeme Owunari Georgewill Iyeopu Minakiri Siminialayi atuboyedia wolfe obianime Department of Pharmacology Faculty of Basic Medical Sciences College of Health Sciences University of Port Harcourt Port Harcourt Nigeria

This research work investigated and compared the chronic renal toxicological profile of disulfiram, copper gluconate and disulfiram/copper gluconate combination, in a 90-day time- and dose-dependent study in rodents. 88 rats weighing an average of 280 g divided into eleven groups consisting of 8 rats each were used for this experiment. The control groups received normal saline as placebo and 99.5% dimethyl sulfoxide (DMSO) (solvent control). Three oral doses (low, medium and high) of disulfiram (18.65 mg/kg, 37.3 mg/kg and 74.6 mg/kg), copper gluconate (3.75 mg/kg, 7.5 mg/kg and 15 mg/kg) and both drugs in combination were administered daily with those of the combination given 12 hours apart. Blood samples were collected via cardiac puncture in heparinised bottles and centrifuged, and the serum was decanted on 30, 45, 60 and 90 days for analysis. Renal function parameters—electrolytes (Na+, K+), urea and creatinine were evaluated. Results showed significant (p < 0.05) dose- and time-dependent increase in electrolyte level (Na+, K+), blood urea and creatinine respectively. The results are all pointers to the development of renal failure. It therefore appears that the DSF/CG combination is nephrotoxic and this effect is dose-dependent and synergistic.

关键词： Disulfiram Copper Gluconate Renal Function

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