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Spectrophotometer with enhanced sensitivity for uric acid detection

Chinese Optics Letters 2019年第8期17卷 66-70页

作者： nurfatihah Che Abd Rashid Nor Hafizah Ngajikin Asrul Izam azmi Rashidah Arsat Suhaila Isaak Noran Azizan Cholan nur ellina azmi Faculty of Electrical Engineering Universiti Teknologi Malaysia 81310 Johor Bahru Johor Malaysia Faculty of Electrical and Electronic Engineering Universiti Tun Hussein Onn Malaysia 86400 Bt. Pahat Johor Malaysia Industrial Biotechnology Research Centre SIRIM Berhad 40200 Shah Alam Selangor Malaysia

A spectrophotometer with an LED as the light source for uric acid detection is proposed in this work. The mechanism of uric acid detection is based on energy absorbed by sodium urate, which is a chemical product of uric acid and sodium hydroxide solution. For the performance validation, comparison between the spectrophotometer with an LED and halogen lamp is carried out. Measurement results suggest that the spectrophotometer system with LED light has better sensitivity than that with halogen light. At a 460 nm wavelength,the sensitivity for the spectrophotometer with an LED is 0.0046 dL/mg, which is 73% higher than that with halogen light that records 0.0012 dL/mg. This enhanced sensitivity is attributed to the higher luminous efficacy of the LED light beam. As a result, a larger amount of flux interacts with the sample, leading to the sensitivity enhancement. The spectrophotometer with an LED is also applied for the detection of uric acid in a real human urine sample. Based on the experimental data at a 460 nm wavelength, the method manages to achieve the sensitivity of 0.0016 dL/mg, accuracy of 96.01%, limit of detection of 4.79 mg/dL, and limit of quantification of 14.52 mg/dL. These findings show that the use of LED as the input light source is promising for the spectrophotometer.

关键词： spectrophotometer mechanism show

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An Optical Based Biosensor for the Determination of Ammonium in Aqueous Environment

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American Journal of Analytical Chemistry 2012年第5期3卷 364-370页

作者： nur ellina azmi Jaafar Abdullah Musa Ahmad Hamidah Sidek Lee Yook Heng Samsulida Abd Rahman Industrial Biotechnology Research Centre SIRIM Berhad Shah Alam Malaysia Industrial Biotechnology Research Centre SIRIM Berhad Shah Alam Malaysia. School of Chemical Sciences and Food Technology Faculty of Sciences and Technology Universiti Kebangsaan Malaysia Bangi Malaysia. School of Chemical Sciences and Food Technology Faculty of Sciences and Technology Universiti Kebangsaan Malaysia Bangi Malaysia.

A simple and rapid optical biosensor for the determination of ammonium was developed by immobilization of gluta-mate dehydrogenase (GLDH) and diaphorase (Dph) in chitosan film coated on a glass slide employing thiazolyl blue tetrazolium bromide (MTT) as a color indicator. The developed biosensor displays a purple color formation of formazan attributed to the unreacted NADH in the reaction system in the presence of ammonium. The color intensity was found to decrease proportionally with the increase of ammonium concentrations after 10 min exposure. The linearity of the biosensor towards ammonium was in the range of 16.8 – 70 μM (R2 = 0.9955) with detection limit of 11 μM. A good agreement (R2 = 0.9984) with indothymol method was obtained in the measurement of fish pond water samples. The effect of potential interferences such as metals ion has also been evaluated.

关键词： Optical Biosensor Glutamate Dehydrogenase Diaphorase Ammonium

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