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多种转谷氨酰胺酶在人结肠的表达差异:溃疡性结肠炎中受损角化细胞转谷氨酰胺酶的表达

世界核心医学期刊文摘（胃肠病学分册） 2005年第8期1卷 41-42页

作者： D’Argenio G Calvani m Della Valle N G.peluso 李翔 National Cance r Institute-G Pascale FoundationDepartment of Experimental Oncology Naples Italy.Prof

Background and aims: Ulcerative colitis (UC) is characterised by refractory in flammatory ulceration and damage to the *** mechanisms underlying impaired healing have yet to be defined. As transglutaminase expression resulting in mat rix protein cross linking is associated with increased wound healing in a rat mo del of colitis, we hypothesised that different types of transglutaminase might a lso play a role in UC. patients end methods: Endoscopic and histological indices were studied in 26 patients with UC (10 active and 16 inactive) and in 20 norma l controls undergoing colonoscopy. Transglutaminase activity was evaluated in pl asma (factor XIIIa) by a radioenzymatic metho- d. Factor XIIIa, tissue and keratinocyte transglutaminase protein content, and mRNA expression in the colon were evaluated by western blot analysis and semiqu antitative reverse transcription-polymerase chain reaction (RT-pCR),respectively. Colon ic location of transglutaminases and their reaction products, the -(γ-glutamy l)lysine bonds, was evaluated by immunohistochemistry using specific monoclonal ***: Transglutaminase activity was significantly lower in the pla sma of patients with active UC (4.2 (2.4) mU/ml;pmU/ml). As shown by we stern blot,protein levels of tissue transglutaminase and factor XIIIa were uncha nged in active UC compared with inactive disease and controls, while the keratin ocyte form was reduced in active UC. Tissue transglutaminase and factor XIIIa im munostaining was strongly present in damaged areas colocalising with isopeptide bonds. In contrast, the keratinocyte form was almost absent in active UC and loc alised in the upper part of the crypts in normal subjects. RT-pCR showed upregu lation of tissue transglutaminase mRNA in active UC (320%compared with controls ) while keratinocyte transglutaminase gene expression was downregulated in activ e UC. Conclusion

关键词：溃疡性结肠炎结肠镜检角化细胞转谷氨酰胺酶大鼠结肠炎模型炎性溃疡活动型组织形态学肠隐窝组织型

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维普期刊数据库

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吸烟后动脉僵硬度和波反射的种族差异

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世界核心医学期刊文摘（心脏病学分册） 2006年第9期2卷 49-50页

作者： Lemogoum D. Van Bortel L. Leeman m. 朱冰坡 Hypertension Clinic Department of CardiologyErasme Hospital808 Lennik Road1070 BrusselsBelgium.Dr

Background: Smoking increases plasma nicotine. Nicotine releases catecholamine s and alters arterial distensibility. The nicotine intake per cigarette is great er and serum cotinine levels, the proximate metabolite of nicotine, are higher i n Blacks than in Whites. We tested the hypothesis that cigarette smoking increas es the pulse wave velocity(pWV), a marker of arterial stiffness, and the augment ation index(AI), a measure of wave reflection, more in Blacks than in Whites. me thods: We matched Black(n=30) and White(n=30) smokers for age, gender, body mass index and height. We determined carotid-femoral pWV(pWVCF) and carotid-radial pWV(pWVCR)(Complior), the AI derived from the aortic pressure waveform(applanat ion tonometry, Sphygmocor), blood pressure, heart rate(HR) and cotinine levels b efore and after cigarette smoking. We also performed measurements in 16 participants aft er sham smoking. Results: Smoking increased the AI, pWVCF and pWVCR in the whole population(all ppWV were positively rela ted to serum cotinine levels(all pmoking increased serum cotinine(p=0. 01) and mean blood pressure(p=0.03) more,but raised the HR to a lesser extent, i n Blacks[+8±4 versus+13±6 beats/ min in Whites(mean±SD), p=0.01]. Blacks di sclosed larger increases in AI adjusted for HR(Blacks, +7.2±8 versus Whites, +4.4±8%; p=0.03), pWVCF(Blacks, +1.1±0.2 versus Whites, +0.6±0.3 m/s; ppWVCR(Blacks, +1.4±0.1 versus Whites, +0.7±0.4 m/s; pnorma lized for the mean blood pressure. No changes were observed with sham smoking. C onclusions: Smoking acutely increases the pWV and AI in Blacks more than in Whit es. Differences in nicotine metabolism and β-adrenergic sensitivity could expl ain these findings.

关键词：动脉僵硬度吸烟者波反射种族差异儿茶酚胺类物质平均血压脉搏波速度脉搏压力波

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维普期刊数据库

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肌萎缩侧索硬化患者和正常受试者单个运动单位阈刺激的变异性

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世界核心医学期刊文摘（神经病学分册） 2005年第3期1卷 47-47页

作者： Jillapalli D. Shefner J.m. 夏峰 Department of Neurology Upstate Medical University 750 E. Adams Street Syracuse NY 13210 United States Dr.

Repetitive nerve stimulation often shows responses with an abnormal decrement in patients with amyotrophic lateral sclerosis (ALS), suggesting instability of the neuromuscular junction; however, the pathophysiology and response characteri stics of this instability are not clear. We evaluated response variability of 47 single motor units from 16 patients with ALS and 51 units from 10 normal subjec ts, acquired by delivering threshold stimuli sporadically at 0.5 Hz or less. In addition, in 46 other different single motor units obtained from 21 patients wit h ALS, variability was studied at both 1 and 3 Hz stimulation rates. motor uni ts from patients with ALS were significantly more variable than those from norma l subjects, even when their larger amplitude was accounted for. This increased v ariability was not rate dependent. Response variability is a critical measure in the statistical method of motor unit number estimation and is attributed to var iability in the number of units activated; the fact that variability of single m otor units varies with disease state may be a potentially confounding factor in the application of the technique.

关键词：肌萎缩侧索硬化阈刺激运动单位正常受试者神经肌肉接头刺激频率频率依赖性神经刺激反应特征病理生理

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维普期刊数据库

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外观健康的癌症生存者的运动能力

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世界核心医学期刊文摘（儿科学分册） 2006年第5期2卷 9-9页

作者： De Caro E. Fioredda F. Calevo m.G. G. pongiglione 张振 Dept. of Cardiology G. Gaslini Children's Hospital Largo Gerolamo Gaslini 5 16148 Genoa Italy.Dr

Aims: To evaluate cardiopulmonary exercise tolerance in a large cohort of appa rently healthy paediatric cancer survivors in order to determine their participa tion in sporting activities. methods: A total of 84 young (mptomatic childhood cancer survivors, who had been exposed to anthracyclines ( mean dose 212 mg/m2) and/or chest irradiation (median dose 2000 cGy), with norma l left ventricular systolic function at rest (fractional shortening > 29%), and 79 healthy controls were studied. Exercise testing was performed on a treadmill ergometer. Gas exchange analysis and derived variables were measured on a breat h-by-breath basis. pulmonary functional evaluation was performed before exerci se. Echocardiographic evaluation at rest was performed within one month before t he exercise test. Results: There were no differences in exercise responses betwe en patients and controls. In boys mean VO2 max was slightly but sign ificantly lower than in controls. This finding was thought to be a result of dec reased physical fitness as all the other exercise parameters were similar to those in the controls. Conclusions: Results show that apparently healthy survivors of paediatric cancer can take part in dynamic sporting activities if they exhibi t a normal response to cardiopulmonary exercise testing, while those that exhibi t a reduced VO2 max should be re-evaluated after an aerobic training programme, and should undergo tailored dynamic physical activity if the VO2 max does not n ormalise.

关键词：癌症生存者运动能力健康外观左室收缩功能正常左室短轴缩短率平均剂量蒽环类药物体育活动运动耐量

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