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Glycinebetaine enhances the tolerance of tomato plants to high temperature during germination of seeds and growth of seedlings

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Glycinebetaine enhances the tolerance of tomato plants to hi...

Assessing optimal Roux-en-Y reconstruction technique after total gastrectomy using the Postgastrectomy Syndrome Assessment Scale-45

山东植物生理学会第七次代表大会暨植物生物学与现代农业研讨会

作者： Shufen Li Feng Li Jianwei Wang Wen Zhang Qingwei Meng Tony HH Chen norio Murata Xinghong Yang College of Life Science State Key Laboratory of Crop BiologyShandong Key Laboratory of Crop BiologyShandong Agricultural University

Tomato(Lycopersicon esculentum cv.’Moneymaker’) was transformed with a codA gene,from Arthrobacter globiformis,for choline oxidase that had been modified to allow targeting to both chloroplasts and the ***(GB) accumulated in seeds of transformed plants up to 1μmol g DW,while no detectable GB was found in wild-type *** incubation at temperatures from 25℃to 55℃for 90 min during imbibition,codA-transgenic seeds germinated faster and at higher frequency than wild-type *** the exposure of imbibed seeds to 40°/30℃(12 h/12 h) or 34°/34℃(16 h/8 h), transgenic seeds germinated faster and developed more vigorous seedlings than wild-type *** compared the expression of genes for a mitochondrial small heat-shock protein(MT-sHSP),heat-shock protein 70(HSP70),and heat shock cognate 70(HSC70) between wild-type and transgenic seeds after heat stress at 34℃.Levels of expression of all three genes were higher in transgenic seeds than in wild-type seeds during heat stress,and the accumulation of HSP70 was more prominent in codA-transgenic seeds than in wild-type *** of GB at 0.1 mM to 5 mM to the germination medium or imbibition of seeds in a solution of GB enhanced the tolerance of wild-type seeds to high ***-type seeds that had been soaked in 1 mM GB for 20 h before high-temperature treatment also expressed heat-shock genes at elevated levels and accumulated more HSP70 than *** results suggest that GB,either applied exogenously or accumulated in vivo in codA-transgenic seeds,enhanced the expression of heat-shock genes in and improved the tolerance to high temperature of tomato seeds during germination.

关键词： Glycinebetaine heat-shock protein high temperature seed germination tomato

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World Journal of Clinical Oncology 2022年第5期13卷 376-387页

作者： Masami Ikeda Masashi Yoshida norio Mitsumori Tsuyoshi Etoh Chikashi Shibata Masanori Terashima Junya Fujita Kazuaki Tanabe Nobuhiro Takiguchi Atsushi Oshio Koji Nakada Department of Surgery Asama General HospitalNagano 385-8558Japan Department of Surgery International University of Health and Welfare HospitalTochigi 329-2763Japan Department of Surgery The Jikei University School of MedicineTokyo 105-8461Japan Department of Gastroenterological Surgery Oita UniversityOita 879-5593Japan Department of Surgery Tohoku Medical and Pharmaceutical UniversityMiyagi 983-8512Japan Division of Gastric Surgery Shizuoka Cancer CenterShizuoka411-8777Japan Department of Surgery Yao Municipal HospitalOsaka 581-0069Japan Department of Gastroenterological and Transplant Surgery Institute of Biomedical and Health SciencesHiroshima UniversityHiroshima 734-8551Japan Department of Gastroenterological Surgery Chiba Cancer CenterChiba 260-8717Japan Faculty of Letters Arts and SciencesWaseda UniversityTokyo 162-8644Japan Department of Laboratory Medicine The Jikei University School of MedicineTokyo 105-8461Japan

BACKGROUND Following a total gastrectomy,patients suffer the most severe form of postgastrectomy *** is a significant clinical problem as it reduces quality of life(QOL).Roux-en-Y reconstruction,which is regarded as the gold standard for post-total gastrectomy reconstruction,can be performed using various *** the technique used could affect postoperative QOL,there are no previous reports regarding the *** To investigate the effect of different techniques on postoperative *** data was collected from the registry of the postgastrectomy syndrome assessment study(PGSAS).METHODS In the present study,we analyzed 393 total gastrectomy patients from those enrolled in *** were divided into groups depending on whether antecolic or retrocolic jejunal elevation was performed,whether the Roux limb was“40 cm”,“shorter”(≤39 cm),or“longer”(≥41 cm),and whether the device used for esophageal and jejunal anastomosis was a circular or linear ***,we comparatively investigated postoperative QOL of the *** Reconstruction route:Esophageal reflux subscale(SS)occurred significantly less frequently in patients who underwent antecolic *** limb length:“Shorter”Roux limb did not facilitate esophageal reflux SS and somewhat attenuated indigestion SS and abdominal pain *** technique:In terms of esophagojejunostomy techniques,no differences were *** The techniques used for total gastrectomy with Roux-en-Y reconstruction significantly affected postoperative *** results suggest that elevating the Roux limb,which is not overly long,through an antecolic route may improve patients’QOL.

关键词： Total gastrectomy Roux-en-Y Postgastrectomy syndrome Quality of life Postgastrectomy Syndrome Assessment Scale-45

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DNA改性聚砜多孔微球选择性清除环境激素

高等学校化学学报 2005年第2期26卷 349-352页

作者：赵长生杨开广野水基义西则雄四川大学高分子科学与工程学院日本北海道大学

将 DNA视为生物大分子材料 ,制成 DNA改性聚砜微球 ,在生物体外考察改性微球对环境激素 ,包括重金属离子的选择性清除 .制备了 2种改性聚砜微球 :DNA共混微球 (将 DNA共混入聚砜溶液 ,当混合溶液滴入水中时 ,发生相分离而沉淀 )和 DNA... 详细信息

将 DNA视为生物大分子材料 ,制成 DNA改性聚砜微球 ,在生物体外考察改性微球对环境激素 ,包括重金属离子的选择性清除 .制备了 2种改性聚砜微球 :DNA共混微球 (将 DNA共混入聚砜溶液 ,当混合溶液滴入水中时 ,发生相分离而沉淀 )和 DNA表面改性聚砜微球 (先制备聚砜多孔微球 ,再通过紫外线照射的方法将 DNA接枝到聚砜微球表面 ) .两种 DNA改性聚砜微球在水中比较稳定 ,在酸或碱性条件下 ,由于DNA水解而不稳定 .对环境激素的清除结果表明 ,DNA改性聚砜微球可以选择性清除包括联苯、苯酚和二恶荚类环境荷尔蒙物质 ;同时选择性吸附锌、铜、银、铅和铬等重金属离子 .

关键词： DNA 聚砜微球环境激素液-液相分离

两种不同方法获取单个海马神经元用于膜片钳技术的比较

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两种不同方法获取单个海马神经元用于膜片钳技术的比较

Feasibility of the Routine Clinical Use of a Multiplex Virus Polymerase Chain Reaction Assay Based on Blood Virus Detection in Hematopoietic Stem Cell-Transplanted Patients

中国解剖学会2002年年会

作者：钱亦华王智明 Akaike norio 西安交通大学医学院人体解剖学教研室第四军医大学人体解剖学教研室日本国九州大学医学研究院

膜片钳技术最早始于七十年代,随着人们对其认识的不断深入,在近十年内,此方法无论在技术上还是在应用上都被赋予了新的内涵。此项技术已被广泛应用于多个领域的研究。机械分离与酶消化两种方法,均可获取单个海马神经元,这是做单细胞膜...

膜片钳技术最早始于七十年代,随着人们对其认识的不断深入,在近十年内,此方法无论在技术上还是在应用上都被赋予了新的内涵。此项技术已被广泛应用于多个领域的研究。机械分离与酶消化两种方法,均可获取单个海马神经元,这是做单细胞膜片钳研究细胞功能的基础。机械分离获取单个海马神经元的方法是近年Akaike norio等人首创,已在世界上被公认;酶消化法是较早公认获取单一细胞的方法。我们采用膜片钳技术结合FMl-43荧光免疫细胞化学技术对

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International Journal of Clinical Medicine 2022年第2期13卷 67-81页

作者： Hiroko Tsunemine Miho Sasaki Yuriko Zushi Toshiharu Saitoh norio Shimizu Yasuhiro Tomaru Yumi Aoyama Ryusuke Yamamoto Tomomi Sakai Nobuyoshi Arima Taiichi Kodaka Takayuki Takahashi Departments of Hematology Shinko Hospital Kobe Japan Cell Therapy Shinko Hospital Kobe Japan Center for Stem Cell and Regenerative Medicine Tokyo Medical and Dental University Tokyo Japan Akasaka Hematology & Oncology Clinic Kobe Japan

Background: Multiplex virus assays are useful in immunocompromised hosts but still challenging in routine clinical settings in terms of their sensitivity, specificity, reproducibility, and time and cost performances. In recent years, we developed a qualitative multiplex virus PCR assay capable of the simultaneous detection of 13 virus species within 3 h. However, because of the multiple and concomitant nature of this virus assay, it should be validated for qualitative reliability. Materials and Methods: As a preclinical examination, this multiplex PCR was able to detect 1.25 × 103 copies/mL of 13 synthesized virus genomes and preserved same virus DNAs by the serial dilution method. Blood samples from 40 patients who underwent hematopoietic stem cell transplantation were then examined by multiplex PCR for 13 virus species, followed by quantitative real-time PCR for all 13 virus species as reference PCR when these patients developed symptoms suggestive of viral infection. Results: In 421 cumulative qualitative-quantitative tests, the multiplex PCR certainly detected 1.0 × 103 copies/mL of 5 viruses (CMV, JCV, BKV, HHV-6, ADV) that were frequently detected and thus reasonably analyzed. The positive and negative predictive values of multiplex PCR were 84.2% - 93.3% and 90.7% - 99.0%, respectively, and sensitivity and specificity were 59.0% - 83.3% and 97.2% - 99.2%, respectively, for these 5 viruses. Conclusion: From these performances, the multiplex PCR assay may be acceptable in a routine clinical laboratory setting.

关键词： Multiplex Virus PCR Assay Routine Laboratory Use Positive Predictive Value Negative Predictive Value Sensitivity Specificity

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最近热处理的进步和设备的开发(二)

钢铁 1984年第12期 66-76页

作者：金武典夫

6.钢轨的热处理在日本,随着重轨铁道、高速铁路的发展,研制了耐磨性优良的改良头部热处理的钢轨,并供实用,发挥着很大的效果[13,14]。

关键词：热处理钢轨感应加热电加热口口

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含锌高炉粉尘脱除燃煤烟道气中的SO_2

化工冶金 2000年第4期21卷 438-442页

作者：杨学民郭占成谢裕生新井纪男中国科学院化工冶金研究所名古屋大学高温能源变换研究中心

研究了含锌高炉粉尘对燃煤产生的烟道气的脱硫行为. 实验结果表明, 在523(823 K温度范围内, 利用含锌高炉粉尘可以脱除烟道气中87%(93%的SO2, 脱硫率随烟道气温度的升高而增加，其脱硫的表观活化能为4.93 kJ/mol. 实验还发现，在1123(12... 详细信息

研究了含锌高炉粉尘对燃煤产生的烟道气的脱硫行为. 实验结果表明, 在523(823 K温度范围内, 利用含锌高炉粉尘可以脱除烟道气中87%(93%的SO2, 脱硫率随烟道气温度的升高而增加，其脱硫的表观活化能为4.93 kJ/mol. 实验还发现，在1123(1273 K的温度范围内提高燃煤温度，含锌高炉粉尘对烟道气脱硫率无明显影响.

关键词：含锌高炉粉尘烟道气碚硫动力学

控制隔震层发生过大位移的连接摩擦阻尼器的参数优化设计

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振动工程学报 2016年第2期29卷 201-206页

作者：邹爽五十子幸树井上范夫福见祐司广州大学工程抗震研究中心广东广州510405 东北大学日本仙台980-0845

为了避免和减轻由过大的隔震层位移引起的损害,提出了一种控制隔震层位移的新型阻尼器—连接摩擦阻尼器。介绍了阻尼器的构造和工作原理,并对其参数进行优化设计。将连接摩擦阻尼器作为大震时控制隔震层过大位移的安全装置来考虑,以一栋... 详细信息

为了避免和减轻由过大的隔震层位移引起的损害,提出了一种控制隔震层位移的新型阻尼器—连接摩擦阻尼器。介绍了阻尼器的构造和工作原理,并对其参数进行优化设计。将连接摩擦阻尼器作为大震时控制隔震层过大位移的安全装置来考虑,以一栋2层的滑移隔震木结构住宅为例,采用序列二次规划法对阻尼器的参数进行优化设计。构造参数优化目标函数和约束条件,求得连接摩擦阻尼器参数最优解。通过算例应用表明该方法在连接摩擦阻尼器减震设计过程中具有较强的实用性和有效性。

关键词：基础隔震摩擦阻尼器参数优化减震控制

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腹腔镜辅助远端胃切除术重建方式的比较

中日友好医院学报 2010年第2期24卷 67-70页

作者：韦佳明黄林平 Seigo Kitano norio Shiraishi 中日友好医院普外一科北京100029 大分大学医学部第一外科

目的:比较早期胃癌患者接受腹腔镜辅助远端胃切除术(LADG)后行Roux-en Y(RY)重建与Billroth Ⅰ(B Ⅰ)重建的短期结果。方法:2002年6月～2007年2月共有96例早期胃癌患者在日本大分大学医院接受了LADG,其中RY重建22例、BⅠ重建74例。比较R... 详细信息

目的:比较早期胃癌患者接受腹腔镜辅助远端胃切除术(LADG)后行Roux-en Y(RY)重建与Billroth Ⅰ(B Ⅰ)重建的短期结果。方法:2002年6月～2007年2月共有96例早期胃癌患者在日本大分大学医院接受了LADG,其中RY重建22例、BⅠ重建74例。比较RY组和BⅠ组患者的临床病理特点、术中情况、并发症、术后经过和血化验结果。结果:2组间术中情况、总并发症率、术后经过和血清学检查结果均无显著性差异(均P>0.05)。2组间手术相关并发症和一般并发症的发生率也无显著性差异(均P>0.05)。RY组有1例吻合口漏和2例胃排空障碍;而BⅠ组有2例吻合口狭窄。结论:LADG术后采用RY重建是安全可行的,具有和BⅠ重建同样的腹腔镜微创手术的优点。

关键词：胃肿瘤远端胃切除术腹腔镜重建