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Inhibition mechanisms of secretome proteins from Paenibacillus polymyxa Kp10 and Lactococcus lactis Gh1 against methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus

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Asian Pacific Journal of Tropical Biomedicine 2022年第11期12卷 483-494页

作者： Nurul Hana Zainal Baharin Nur Fadhilah Khairil Mokhtar Mohd Nasir Mohd Desa Nurul Diana Dzaraly AbdulRahman Muthanna Mazen M.Jamil Al-Obaidi Mohd Hafis Yuswan Sahar Abbasiliasi norasfaliza rahmad Wan Ahmad Kamil Wan Nur Ismah Amalia Mohd Hashim Shuhaimi Mustafa Laboratory of Halal Science Research Halal Products Research InstituteUniversiti Putra Malaysia43400 UPM SerdangSelangorMalaysia Department of Biomedical Sciences Faculty of Medicine and Health SciencesUniversiti Putra Malaysia43400 UPM SerdangSelangorMalaysia Department of Science University of Technology and Applied SciencesRustaq 10 P.C.329Oman Agro-Biotechnology Institute MalaysiaNational Institutes of Biotechnology Malaysia43400 UPM SerdangSelangorMalaysia Department of Microbiology Faculty of Biotechnology and Biomolecular ScienceUniversiti Putra Malaysia43400 UPM SerdangSelangorMalaysia

Objective:To determine the inhibition mechanisms of secretome protein extracted from Paenibacillus polymyxa Kp10(Kp10)and Lactococcus lactis Gh1(Gh1)against methicillin-resistant Staphylococcus aureus(MRSA)and vancomycin-resistant Enterococcus(VRE).Methods:The sensitivity and viability of MRSA and VRE treated with secretome proteins of Kp10 and Gh1 were determined using minimal inhibitory concentration,minimum bactericidal concentration,and time-to-kill *** morphological changes were observed using scanning electron microscopy and transmission electron *** elucidate the antimicrobial mechanism of secretome protein of Kp10 and Gh1 against MRSA and VRE,2D gel proteomic analysis using liquid chromatography-mass spectrometry was run by comparing upregulated and downregulated proteins,and the proton motive force study including the efflux of ATP,pH gradient,and the membrane potential study were ***:MRSA and VRE were sensitive to Kp10 and Gh1 secretome protein extracts and displayed apparent morphological and internal composition *** proteins associated with cellular component functions were either downregulated or upregulated in treated MRSA and VRE by changing the membrane potential ***:Kp10 and Gh1 secretome proteins reduce the growth of VRE and MRSA by damaging the cell *** division,cell wall biosynthesis,and protein synthesis are involved in the inhibition mechanism.

关键词： Antimicrobial Proteins Secretome proteins Antibiotic-resistance Paenibacillus polymyxa Kp10 Lactococcus lactis Gh1 Vancomycin-resistant Enterococcus Methicillin-resistant Staphylococcus aureus Mechanism

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