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Assessment of the Visual Analogue Score in the Evaluation of the Pruritus of Cholestasis

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Journal of Clinical and Translational Hepatology 2017年第3期5卷 203-207页

作者： nora V.Bergasa E.Anthony Jones NYC H + H Metropolitan New York NY USA New York Medical College Valhalla NY USA Department of Gastroenterology and Liver Diseases Academic Medical Center Amsterdam The Netherlands

Background and Aims:A visual analogue score (VAS),based on application of a visual analogue scale,has been widely used to assess pruritus in clinical studies of patients with cholestatic liver disease.A VAS is a numerical score of the severity of the perception of pruritus,and,hence,is inherently *** objective of this study was to assessthe reliability of a VAS as an index of pruritus in cholestatic ***:In 8 patients with chronic pruritus due to primary biliary cholangitis,values for a VAS of pruritus were compared with corresponding measurements of scratching activity,which were generated by a monitoring system specifically designed to quantitate this *** relationship between individual values for the VAS and corresponding values for scratching activity during a specific interval immediately preceding the recording of the VAS was examined by determining the Spearman's rank correlation ***:The mean Spearman's rank correlation coefficient between individual values for the VAS and corresponding mean values for scratching activity was 0.072;the range of these coefficients was-0.04 to 0.26.A VAS of pruritus is an unreliable index of scratching activity,and,hence,of the pathophysiological process responsible for the pruritus of ***:It is concluded that the use of a VAS as a primary quantitative endpoint in trials of the efficacy of potential therapies for the pruritus of cholestasis may be inappropriate.

关键词： Pruritus Cholestasis Visual analogue score Scratching activity Primary biliary cholangitis Quantitative efficacy endpoint

Essential residues for the enzyme activity of ATP-dependent MurE ligase from Mycobacterium tuberculosis

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Protein & Cell 2010年第11期1卷 1011-1022页

作者： Chandrakala Basavannacharya Paul R.Moody Tulika Munshi nora Cronin Nicholas H.Keep Sanjib Bhakta Institute for Structural and Molecular Biology Department of Biological SciencesBirkbeckUniversity of LondonLondon WC1E 7HXUK

The emergence of total drug-resistant tuberculosis(TDRTB)has made the discovery of new therapies for tuberculosis *** cytoplasmic enzymes of peptidoglycan biosynthesis have generated renewed interest as attractive targets for the development of new *** of the cytoplasmic enzymes,uridine diphosphate(UDP)-MurNAc-tripeptide ligase(MurE),catalyses the addition of meso-diaminopimelic acid(m-DAP)into peptidoglycan in Mycobacterium tuberculosis coupled to the hydrolysis of *** of *** MurE were generated by replacing K157,E220,D392,R451 with alanine and N449 with aspartate,and truncating the first 24 amino acid residues at the Nterminus of the *** of the specific activity of these proteins suggested that apart from the 24 Nterminal residues,the other mutated residues are essential for *** in Km values for one or more substrates were observed for all mutants,except the N-terminal truncation mutant,indicating that these residues are involved in binding substrates and form part of the active site *** mutant proteins were also tested for their specificity for a wide range of ***,the mutations K157A,E220A and D392A showed hydrolysis of ATP uncoupled from *** ATP hydrolysis rate was enhanced by at least partial occupation of the uridine nucleotide dipeptide binding *** study provides an insight into the residues essential for the catalytic activity and substrate binding of the ATP-dependent MurE *** ATPdependent MurE ligase is a novel drug target,the understanding of its function may lead to development of novel inhibitors against resistant forms of ***.

关键词： peptidoglycan MurE ligase site-directed mutagenesis m-DAP

Stem cell therapy for the treatment of Leydig cell dysfunction in primary hypogonadism

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World Journal of Stem Cells 2016年第10期8卷 306-315页

作者： Taylor C Peak nora M Haney William Wang Kenneth J DeLay Wayne J Hellstrom Department of Urology School of MedicineTulane University

The production of testosterone occurs within the Leydig cells of the testes. When production fails at this level from either congenital, acquired, or systemic disorders,the result is primary hypogonadism. While numerous testosterone formulations have been developed, none are yet fully capable of replicating the physiological patterns of testosterone secretion. Multiple stem cell therapies to restore androgenic function of the testes are under investigation. Leydig cells derived from bone marrow, adipose tissue, umbilical cord, and the testes have shown promise for future therapy for primary hypogonadism. In particular, the discovery and utilization of a group of progenitor stem cells within the testes, known as stem Leydig cells(SLCs), has led not only to a better understanding of testicular development, but of treatment as well. When combining this with an understanding of the mechanisms that lead to Leydig cell dysfunction, researchers and physicians will be able to develop stem cell therapies that target the specific step in the steroidogenic process that is deficient. The current preclinical studies highlight the complex nature of regenerating this steroidogenic process and the problems remain unresolved. In summary, there appears to be two current directions for stem cell therapy in male primary hypogonadism. The first method involves differentiating adult Leydig cells from stem cells of various origins from bone marrow, adipose, or embryonic sources. The second method involves isolating, identifying, and transplanting stem Leydig cells into testicular tissue. Theoretically, in-vivo re-activation of SLCs in men with primary hypogonadism due to age would be another alternative method to treat hypogonadism while eliminating the need for transplantation.

关键词： Stem cell therapy Leydig cells Primary hypogonadism Stem Leydig cells Testosterone Bone marrow-derived stem cells Adipose-derived mesenchymal stem cells

GC-MS analysis,determination of total phenolics,flavonoid content and free radical scavenging activities of various crude extracts of Moringa peregrina(Forssk.) Fiori leaves

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Asian Pacific Journal of Tropical Biomedicine 2014年第12期4卷 964-970页

作者： Mooza Al-Owaisi nora Al-Hadiwi Shah Alam Khan Department of Pharmacy Oman Medical College

Objective:To perform phytochemical screening,estimate total phenolics,flavonoids and to evaluate antioxidant potential of Moringa peregrina(***) ***:The dried powdered leaves of ***(150 g) were extracted exhaustively by Soxhlet with ethanol and then fractionated into hexane,chloroform,ethy alacetate and *** the prepared extracts were also analyzed by gas chromatography-mass spectrometry to identify and characterize the chemical compounds present in the crude ***- Ciocalteu reagent and aluminium chloride colorimetric methods were used to estimate total phenolic and flavonoid content of *** peroxide and 1,1 diphenyl-2-picrylhydrazyl were used to determine in vitro antioxidant ***:Phytochemical analysis of ethanol extract showed presence of major classes of *** chromatography-mass spectrometry results revealed presence of 19 phytoconstituents in hexane extract,6 in ethyl acetate and 7 compounds in methanolic *** extract was found to contain the highest phenolic content and *** vitro antioxidant activities of all crude extracts were significant and comparable with the standard ascorbic ***:Results of this study show that the leaves of *** are the rich source of phenolic compounds that can play an important role in preventing the progression of many diseases.

关键词： Antioxidant activity Flavonoid content GC-MS analysis Moringa peregrina Total phenolics

Toxoplasma gondii infection among chronic hepatitis C patients:A casecontrol study

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Asian Pacific Journal of Tropical Medicine 2014年第8期7卷 589-593页

作者： Hala A El-Nahas nora L El-Tantawy Raghda E Farag Argaya MA Alsalem Faculty of Medicine Mansoura University Faculty of Science Sebha Unirersity

Objective:To determine the detection rate of anti-Toxoplasma gondii(***) IgG and IgM in chronic HCV patients attending the Department of Tropical Medicine Mansoura University hospital in ***:This study included 120 adult chronic HCV patients.81 decompensate cirrhosis(late-stage)and 39 chronic HCV non cirrhotic patients(early-stage) and40 healthy blood donors as *** samples uere examined for anti-Toxoplasma IgM and anti-Toxoplasma IgG antibodies by ***-time RT-polymerase chain reaction assay was done for quantitation of hepatitis C ***:*** IgG antibodies were detected in 75(92.6%) of 81 late-stage cirrhotic patients.30(76.9%) of the 39 chronic HCV non cirrhotic patients(early-Stage) and in 6(15ft) of 40 controls with statistically significant difference(P<0.001).*** IgM antibodies were found in 11(13.6%) in late stage patients,5(12.8%)in early stage and in 3(7.5%) of controls with no statistical significant difference(P=0.610).There was no correlation between stage of fibrosis and IgM or IgG antibodies positivity in our studied groups(P=0.526).High IgG levels significantly correlated with high viral load(P=0.026).Conclusions:Our findings suggest that the serious opportunistic *** infection represent a potential significant risk for chronic HCV *** should be considered in their investigations and follow-up.

关键词： Toxoplasma gondii HCV ELISA Real-time PCR

Quantitative proteomic determination of diethylstilbestrol action on prostate cancer

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Asian Journal of Andrology 2013年第3期15卷 413-420页

作者： Pierre Bigot Kevin Mouzat Muriel Bahut nora Benhabiles Geraldine Cancel Tassin Abdel-Rahmene Azzouzi Olivier Cussenot Department of Urology Angers University Hospital Angers 49933 France CeRePP Tenon Hospital Paris 75970 France Department of Biochemistry N'imes University Hospital NTmes 30029 France Plate-Forme Technologique de Biotechnologie Moleculaire Angers University Angers 49933 France 5CEA DSV IRCM SREIT Laboratoire de Cancerologie Experimentale Fontenay-aux-Roses 92265 France

Diethylstilbestrol （DES） has a direct cellular mechanism inhibition on prostate cancer. Its action is independent from the oestrogen receptors and is preserved after a first-line hormonal therapy. We aimed to identify proteins involved in the direct cellular inhibition effects of DES on prostate cancer. We used a clonogenic assay to establish the median lethal concentration of DES on 22RV1 cells. 22RV1 cells were exposed to standard and DES-enriched medium. After extraction, protein expression levels were obtained by two-dimensional differential in-gel electrophoresis （2D-DIGE） and isotope labelling tags for relative and absolute quantification （iTRAQ）. Proteins of interest were analysed by quantitative RT-PCR and western blotting. The differentially regulated proteins （P〈0.01） were interrogated against a global molecular network based on the ingenuity knowledge base. The 2D-DIGE analyses revealed DES-induced expression changes for 14 proteins （〉 1.3 fold; P〈0.05）. The iTRAQ analyses allowed the identification of 895 proteins. Among these proteins, 65 had a modified expression due to DES exposure （i.e., 23 overexpressed and 42 underexpressed）. Most of these proteins were implicated in apoptosis and redox processes and had a predicted mitochondrial expression. Additionally, ingenuity pathway analysis placed the OAT and HSBP1 genes at the centre of a highly significant network. RT-PCR confirmed the overexpression of OAT （P=0.006） and HSPB1 （P=0.046）.

关键词： cultured cells DES diethylstilbestrol ingenuity pathway analysis isotope labelling mass spectrometry prostate cancer proteomics

In vitro toxicity of particulate matter emissions from residential pellet combustion

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Journal of Environmental Sciences 2022年第5期34卷 215-226页

作者： Estela D.Vicente Daniela Figueiredo Catia Goncalves Isabel Lopes Helena Oliveira nora Kovats Teresa Pinheiro Celia A.Alves Department of Environment and Planning and CESAM-Centre for Environmental and Marine Studies University of Aveiro3810-193 AveiroPortugal Department of Biology and CESAM-Centre for Environmental and Marine Studies University of Aveiro3810-193 AveiroPortugal Centre of Natural Environmental Sciences University of PannoniaEgyetem str.108200 VeszpremHungary Instituto de Bioengenharia e Biociencias Departamento de Engenharia e Ciencias NuclearesInstituto Superior TecnicoUniversidade de LisboaAv.Rovisco Pais 11049-001 LisboaPortugal

Particulate matter emissions(PM10)from the combustion,in a residential stove,of two com-mercial brands of certified(ENplus A1)pellets,a non-certified brand and laboratory made pellets of acacia were tested for their ability to induce ecotoxic,cytotoxic,and mutagenic re-sponses in unicellular organisms and a human cell *** was evaluated through the Vibrio fischeri bioluminescence inhibition ***,cytotoxicity was assessed at two time points(24-and 48-hr)through two complementary techniques in order to evaluate the cellular metabolic activity and membrane integrity of human lung epithelial cells *** Ames test using two Salmonella typhimurium strains(TA100 and TA98)was employed to assess the mutagenic potential of the polycyclic aromatic hydrocarbon fraction extracted from the PM10 *** obtained with the bioluminescent bacteria indicated that only particles from the combustion of acacia pellets were *** samples induced im-pairment on the A549 cells metabolic activity,while no significant release of lactate dehy-drogenase was ***10 emissions from acacia pellets were the most cytotoxic,while samples from both certified pellets evoked significant cytotoxicity at lower ***-icity time-dependency was only observed for PM10 from the combustion of acacia pellets and one of the brands of certified *** activity was not detected in both *** murium *** study emphasises the role of the raw material for pellet manu-facturing on the toxicological profile of PM *** raw materials should be deeply investigated before their use in pelletisation and combustion in residential appli-ances.

关键词： Ecotoxicity Cytotoxicity Mutagenicity Fuel quality Pellet combustion PM10 emissions

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计算机模拟阴离子对碳酸镍结晶过程的影响

无机化学学报 2009年第10期25卷 1823-1828页

作者：刘昉 nora H. de Leeuw 张昭四川大学化学工程学院成都610065 Department of Chemistry University College of London London WC1H 0AJ UK

本文采用原子模拟技术,研究了不同溶液中碳酸镍的结晶行为。结果表明,水化作用使碳酸镍晶体更稳定,形貌更规整。杂质阴离子SO42-与碳酸镍晶体表面的作用力较强,但对其形貌影响不大。Cl-对表面的作用力较弱,但使晶体形貌更不规则。在模... 详细信息

本文采用原子模拟技术,研究了不同溶液中碳酸镍的结晶行为。结果表明,水化作用使碳酸镍晶体更稳定,形貌更规整。杂质阴离子SO42-与碳酸镍晶体表面的作用力较强,但对其形貌影响不大。Cl-对表面的作用力较弱,但使晶体形貌更不规则。在模拟的几种情况中,碳酸镍的(104)晶面均是最主要的显露面。模拟结果能与实验现象很好吻合。

关键词：计算机模拟碳酸镍结晶过程阴离子影响

Incremental Learning Framework for Mining Big Data Stream

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Computers, Materials & Continua 2022年第5期71卷 2901-2921页

作者： Alaa Eisa nora E.L-Rashidy Mohammad Dahman Alshehri Hazem M.El-bakry Samir Abdelrazek Information Systems Department Faculty of Computers and InformationMansoura UniversityMansoura35516Egypt Machine Learning and Information Retrieval Department Faculty of Artificial IntelligenceKafrelsheikh UniversityKafr El-SheikhEgypt Department of Computer Science College of Computers and Information TechnologyTaif UniversityTaif21944Saudi Arabia

At this current time,data stream classification plays a key role in big data analytics due to its enormous *** of the existing classification methods used ensemble learning,which is trustworthy but these methods are not effective to face the issues of learning from imbalanced big data,it also supposes that all data are *** weakness of current methods is that it takes a long evaluation time when the target data stream contains a high number of *** main objective of this research is to develop a new method for incremental learning based on the proposed ant lion fuzzy-generative adversarial network *** proposed model is implemented in spark *** each data stream,the class output is computed at slave nodes by training a generative adversarial network with the back propagation error based on fuzzy bound *** method overcomes the limitations of existing methods as it can classify data streams that are slightly or completely unlabeled data and providing high scalability and *** results show that the proposed model outperforms stateof-the-art performance in terms of accuracy(0.861)precision(0.9328)and minimal MSE(0.0416).

关键词： Ant lion optimization(ALO) big data stream generative adversarial network(GAN) incremental learning renyi entropy