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Establishment of Human Neuroblastoma Cell-Line (SK-N-SH) as an In Vitro Model of Morphine Addiction

Journal of Pharmacy and Pharmacology 2021年第3期9卷 115-121页

作者： noor Azuin Suliman noor azlina abu bakar Mohamad Aris Mohd Moklas Che Norma Mat Taib Faculty of Medicine and Health Science Universiti Sultan Zainal Abidin(UniSZA)Kampus KotaJalan Sultan MahmudKuala Terengganu20400Malaysia Department of Biomedical Sciences Faculty of Medicine and Health SciencesUniversiti Putra Malaysia43400 UPM SerdangSelangorMalaysia

Morphine is a schedule II-controlled substance that used to allow the diminution of intra-operative, post-operative orchronic pain. However, its usage is limited due to addiction and overdose liabilities. Morphine was observed to cause tolerance,dependence and withdrawal in human. Justification: to date lack of scientific evidence of morphine addiction was carried out byusing specific single human neuroblastoma cell-line (SK-N-SH). Therefore, this study was performed to establish the morphineaddiction model in this cell line. The cells were exposed to morphine for 24 hrs before treatment with methadone, as ananti-withdrawal drug for subsequence 24 hours. The cytosolic fraction of the cell was used in different objectives including receptoraffinity, withdrawal properties, endocytic machinery, desensitisation or internalisation and cellular adaptation. The result shows thatmorphine and methadone bind to the μ-opioid receptor. The morphine-treated cells were observed to increase the expression ofaddiction markers, have a low rate of the endocytic machinery, cause desensitisation of receptor and reduce cellular *** changes by morphine were normalised by the treatment of methadone. As a whole, it is postulated that neuroblastoma cell line,SK-N-SH, can be used as an in-vitro model to demonstrate morphine addiction before animal and human testing.

关键词： Morphine addiction receptor affinity withdrawal properties endocytic machinery desensitisation internalisation cellular adaptation methadone.

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Prevalence and risk factors associated with metabolic dysfunctionassociated steatohepatitis in patients with Helicobacter pylori infection: A population-based study

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World Journal of Hepatology 2024年第10期16卷 1169-1176页

作者： Rashid Abdel-Razeq Lynn Bitar Elio R Bitar Chidera Onwuzo Mohamad-noor abu-Hammour Barish Eren Islam Mohamed Adejoke Johnson Antoine Boustany Somtochukwu Onwuzo Imad Asaad Department of Internal Medicine Cleveland Clinic FoundationClevelandOH 44111United States Faculty of Medicine Saint Joseph University of BeirutBeirut 11042020Lebanon Faculty of Medicine American University of BeirutBeirut 11072020Lebanon Department of Medicine&Surgery General Hospital Lagos IslandLagos Island 101223LagosNigeria Department of Internal Medicine Jacobi Medical Center/North Central Bronx HospitalBronxNY 10461United States Division of Gastroenterology Department of Internal MedicineUniversity of Florida College of MedicineJacksonvilleFL 32209United States Department of Gastroenterology Allegheny General HospitalPittsburghPA 15212United States Department of Gastroenterology Firelands HealthSanduskyOH 44870United States

BACKGROUND Helicobacter pylori(***)is associated with the development of gastrointestinal disorders ranging from gastritis to gastric *** evidence of the association between metabolic dysfunction-associated steatohepatitis(MASH)and *** infection in the literature is ***,we aim to evaluate the risk of developing MASH in patients who have had a diagnosis of *** infection independently of any confounding *** To evaluate the risk of developing MASH in patients who have had a diagnosis of *** *** This study used a validated multicenter research database of over 360 hospitals across 26 healthcare systems across the United States from 1999 to *** regression analysis assessed the risk of developing MASH,adjusting for confounders including *** infection,obesity,type 2 diabetes,hypertension,dyslipidemia,and male gender.A two-sided P value<0.05 was considered as statistically significant,and all statistical analyses were performed using R version 4.0.2(R Foundation for Statistical Computing,Vienna,Austria,2008).RESULTS A total of 79476132 individuals were screened in the database and 69232620 were selected in the final analysis after accounting for inclusion and exclusion ***(14.30%),patients with hyperlipidemia(70.35%),hypertension(73.86%),diabetes mellitus type 2(56.46%),and obese patients(58.15%)were more common in patients with MASH compared to *** a multivariate regression analysis,the risk of MASH was increased in diabetics[odds ratio(OR):3.55;95%CI:3.48-3.62],obese(OR:5.93;95%CI:5.81-6.04),males(OR:1.49;95%CI:1.46-1.52),individuals with hyperlipidemia(OR:2.43;95%CI:2.38-2.49)and *** infection(OR:2.51;95%CI:2.31-2.73).CONCLUSION This is the largest population-based study in the United States illustrating an increased prevalence and odds of developing MASH in patients with *** infection after adjusting for risk factors.

关键词： Metabolic dysfunction-associated steatohepatitis Metabolic dysfunction-associated steatotic liver disease Helicobacter Pylori Cirrhosis Liver

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Advances on ultra-sensitive electrospun nanostructured electrochemical and colori metric sensors for diabetes mellitus detection

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Nano Materials Science 2021年第4期3卷 321-343页

作者： Mohamed Hasaan Hussain Lim Ping Fook Maria Kana Sanira Putri Huey Ling Tan noor Fitrah abu bakar Norbert Radacsi School of Chemical Bngineering College of Engineering University Teknologi MARA 40450 Shah AlamSelangorMalaysia School of Engineering Institute for Materials and ProcessesThe University of EdinburghRobert Stevenson RoadEdinburghEH93FBUnited Kingdom

Recent developments in the biochemical and medicinal industries have been heavily focused on producing affordable glucose biosensors due to the condi nuous annual increase of diabetic patients *** devel-opment of a fast,accurate,and reliable glucose sensor will increase confidence in controlling di abetes mellitus and its assoclated health complications among the diabetic *** is a versatile method that can produce complex nanofbrous assemblies with attractiwe and functional characteristics from varlous *** nanofibers demonstrated high efficiency in the immobilization of biological molecules,which can improve the sensing performance *** ation of polymer electrospun nanofibers with metal nanoparticles,metal oxde or transition metal in producing nanobiocomposites is also a highly popul ar approach in the past few *** report presents the current progress and research trends of the technique,focusing on varous ma-terials and fabrication strategies used to produce biosensing *** helps readers decide the suitable approach in designing highly sensitive,selective,fast,and inexpensive glucose sensors.

关键词： Glucose sensor Electrospinning Nanofiber Electrochemical sensing Colorimetric sensing

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Growth-controlled synthesis of polymer-coated colloidal-gold nanoparticles using electrospray-based chemical reduction

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Particuology 2021年第4期19卷 72-81页

作者： Mohamed Hasaan Hussain noor Fitrah abu bakar Kim-Fatt Low Ana Najwa Mustapa Fatmawati Adam Mohd Nazli Naim I.Wuled Lenggoroe Faculty of Chemical Engineering Universiti Teknologi MARAShah AlamSelangor 40450Malaysia Faculty of Applied Sciences Universiti Teknologi MARAPerak BranchTapah CampusTapah RoadPerak 35400Malaysia Faculty of Chemical and Process Engineering Technology Universiti Malaysia PahangLebuh Raya Tun RazakGambangPahang 26300Malaysia Department of Process and Food Engineering Faculty of EngineeringUniversiti Putra MalaysiaSerdangSelangor 43400Malaysia Graduate School of Bio-Applications and Systems Engineering Tokyo University of Agriculture and TechnologyNaka Cho2-24-16KoganeiTokyo 184-8588Japan Department Chemical of Engineering Tokyo University of Agriculture and TechnologyNaka Cho2-24-16KoganeiTokyo 184-8588Japan

In this study,the controlled nucleation and growth of gold nanoparticles(GNPs)were investigated using a self-repelled mist in a liquid chemical reaction *** electrospray-based chemical reduction method was conducted in the aqueous region and at room temperature to synthesize the polymeric-stabilized gold *** electrospray technique was used to atomize a hydrogen tetrachloraurate(III)(HAuCl4)precursor solution into electrostatically charged *** atomized droplets were dispersed in an aqueous reaction bath containing L-ascorbic acid as a reducing agent and polyvinylpyrrolidone(PVP)as a *** effect of the electrospray parameters,specifically the flow rate and electrospray droplet size,as well as the reaction conditions such as the concentration of reactants,pH,and stabilizer(PVP),were *** mean diameter of the GNPs increased from around 4 to 9 nm with an increase in the electrospray flow rate,droplet size,and current passing through the electrospray *** and monodispersed GNPs were synthesized at a relatively high flow rate of 2 mL/h and a moderate concentration of 2 mM of precursor *** smallest-sized GNP with a high monodispersity was obtained in the reaction bath at a high pH of 10.5 and in the presence of *** is expected that continuous and mass production of the engineered GNPs and other noble metal nanoparticles could be established for scaling up nanoparticle production via the proposed electrospray-based chemical reduction method.

关键词： Electrospray Gold nanoparticles Chemical reduction method L-ascorbic acid PVP Aqueous medium

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Professional values in nursing students in Jordan:baccalaureate nursing students’perception

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Frontiers of Nursing 2022年第3期9卷 319-328页

作者： Rabia S.Allari Nasser I.abu-El-noor Mysoon K.abu-El-noor Faculty of Nursing Al-Ahliyya Amman UniversityJordan Faculty of Nursing Islamic University of GazaPalestine

Objective:Baccalaureate degree curricula consist of several professional values required for nursing students,which are taught in many *** study aimed to explore the perception of baccalaureate Jordanian nursing students regarding professional values and to assess the relationship between students’demographics and their perception of professional ***:The study design was a descriptive,cross-sectional correlational design.A convenience sample of 182 Jordanian undergraduate nursing students enrolled at one of the most prominent universities in Jordan who completed and submitted a self-administered Nurses’Professional Values Scale(NPVS)was considered for this ***:The NPVS total score ranged from 37 to 130,with a relatively high total score mean(99.68 with a mean of 3.83 out of 5).The highest score domain in NPVS was“Justice”with a mean of 3.97,while the lowest ranked domain was“Activism”with a mean of *** correlation coefficient showed no statistically significant correlation between age nor accumulative students’average with a total score of Nurses Professional Values Scale-revised(NPVS-R)and its *** t test results showed that gender and type of students’enrollment(regular ***)did not affect the means of NPVS-R and its *** differences were found to be statistically significant between the means of first year students and fourth year students in the total score of ***:Findings of the study alert nursing educators to pay more attention to the importance of promoting the values related to“activism”and“professionalism”among nursing *** for enhancing the roles of peer supervision and providing peer review to colleagues would contribute to improving nursing students’performance and raising self-awareness because when nursing educators pay more attention to the items of these domains,better future nursing professionals,leaders,and researchers can be ***,understanding nursin

关键词： activism caring justice nursing professional values professionalism student values trust

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A system for detection of cervical precancerous in field emission scanning electron microscope images using texture features

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Journal of Innovative Optical Health Sciences 2017年第2期10卷 81-92页

作者： Yessi Jusman Siew-Cheok Ng Khairunnisa Hasikin Rahmadi Kurnia noor Azuan abu Osman Kean Hooi Teoh Department of Biomedical Engineering Faculty of Engineering University of Malaya50603 Kuala LumpurMalaysia Department of Informatics Engineering Faculty of Engineering Universitas A bdurrab28291 PekanbaruRiauIndonesia Department of Electrical Engineering Faculty of Engineering Andalas UniversityLimau Manis Campus25163 Padang Sumatera BaratIndonesia Department of Pathology Faculty of Medicine University of Malaya50603 Kuala LumpurMalaysia

This study develops a novel cervical precancerous detection system by using texture analysis of field emission scanning electron microscopy(FE-SEM)*** processing scheme adopted in the proposed system focused on two *** first step was to enhance cervical cell FE-SEM images in order to show the precancerous characterization indicator.A problem arises from the question of how to extract features which characterize cervical precancerous *** the first step,a preprocessing technique called intensity transformation and morphological operation(ITMO)algorithm used to enhance the quality of images was *** algo-rithm consisted of contrast stretching and morphological opening *** second step was to characterize the cervical cells to three classes,namely normal,low grade intra-epithelial squamous lesion(LSIL),and high grade intra-epithelial squamous lesion(HSIL).To differen-tiate between normal and precancerous cells of the cervical cell FE-SEM images,human papillomavirus(HPV)contained in the surface of cells were used as *** this paper,we investigated the use of texture as a tool in determining precancerous cell images based on the observation that cell images have a distinct visual *** level co-occurrences matrix(GLCM)technique was used to extract the texture *** confirm the system's perfor-mance,the system was tested using 150 cervical cell FE-SEM *** results showed that the accuracy,sensitivity and specificity of the proposed system are 95.7%,95.7%and 95.8%,respectively.

关键词： Cervical cancer detection electron image image processing features extraction intelligent system.

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Molecular Detection of Mutations within the Quinolone Resistance-Determining Regions in Non Typhoidal Salmonella Isolates from Malaysia

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Health 2017年第4期9卷 640-648页

作者： Fadzilah Mohd-Nor noor Aini abu-bakar Norzilawati Mohd-Isa Zubaidah A. Wahab Zaini Mohd-Zain Microbiology Discipline Faculty of Medicine UniversitiTeknologi MARA Sg. Buloh Campus Jalan Hospital Sg. Buloh Selangor Malaysia Community of Research (CoRE) of Health & Wellbeing UniversitiTeknologi MARA Shah Alam Selangor Malaysia Microbiology Unit Department of Pathology Hospital Sg. Buloh Sg. Buloh Selangor Malaysia

Introduction: The efficacy of chemotherapy in bacteraemia caused by non-typhoidal Salmonella (NTS) is compromised by antibiotic resistance. Objective: This study was undertaken to describe the mechanism of resistance among clinical NTS isolates. Materials & Methodology: Thirty of NTS were isolated from blood (n = 19), stool (n = 10) and bronchioalveolar lavage (BAL;n = 1) respectively. These isolates were tested for susceptibility testing by disc diffusion method against ampicillin, gentamicin, tetracycline, co-trimoxazole, nalidixic acid, ciprofloxacin and ceftriaxone. Epsilometer tests (E-test) for nalidixic acid and ciprofloxacin were performed for nalidixic acid resistant isolates by disc diffusion method. DNA sequencing was carried out on six of the nalidixic acid resistant Salmonella Enteritidis isolates to identify mutations within quinolones resistance determining regions (QRDR) of gyrA, gyrB, parC and parE genes. Results: Resistance rates of NTS isolates from blood, stool, and BAL were respectively 37%, 20% and 0% for ampicillin, 79%, 40% and 0% for tetracycline, 32%, 40% and 0% for co-trimoxazole, 37%, 10% and 100% for nalidixic acid. Eight isolates were resistant to nalidixic acid and had exhibited reduced susceptibility towards ciprofloxacin by E-test. Mutation within QRDR was detected in gyrA gene (n = 6;Asp 47 → His [3], Asp 51 → Asn [1], Asp 73 → Gly [1], and Gly 48 → Asp [1]) and double mutation was detected in parE gene (n = 3;Gly 48 → Asp [3], Glu 82 → Ser [3]). Out of six isolates, three isolates were found to have both gyrA and parE gene mutations. Conclusions: There was no mutation observed in gyrB and parC gene. Mutation in gyrA gene was sufficient to induce decreased susceptibility to ciprofloxacin. Variation in amino acid sequences are novel, while detection of other gene mutation was uncommon.

关键词： Non-Typhoidal Salmonella Quinolones Resistance-Determining Regions Ciprofloxacin

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Role of the CXCR4-SDF1-HMGB1 pathway in the directional migration of cells and regeneration of affected organs

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World Journal of Stem Cells 2020年第9期12卷 938-951页

作者： Nazmul Haque Ismail M Fareez Liew Fong Fong Chanchal Mandal noor Hayaty abu Kasim Kranthi RajaKacharaju Pratiwi Soesilawati Department of Oral Biology and Biomedical Sciences Faculty of DentistryMAHSA UniversitySelangor 42610Malaysia Biotechnology and Genetic Engineering Discipline Life ScienceKhulna UniversityKhulna 9208Bangladesh Faculty of Dentistry University Kebangsaan MalaysiaKuala Lumpur 50300Malaysia Faculty of Dental Medicine Universitas AirlanggaSurabaya 411007Indonesia Department of Conservative Dentistry Faculty of Dentistry MAHSA UniversitySelangor 42610Malaysia Department of Oral Biology Faculty of Dental MedicineUniversitas AirlanggaSurabaya 60115Indonesia

In recent years,several studies have reported positive outcomes of cell-based therapies despite insufficient engraftment of transplanted *** findings have created a huge interest in the regenerative potential of paracrine factors released from transplanted stem or progenitor ***,this notion has also led scientists to question the role of proteins in the secretome produced by cells,tissues or organisms under certain conditions or at a particular time of regenerative *** studies have revealed that the secretomes derived from different cell types contain paracrine factors that could help to prevent apoptosis and induce proliferation of cells residing within the tissues of affected *** could also facilitate the migration of immune,progenitor and stem cells within the body to the site of *** these different paracrine factors present within the secretome,researchers have given proper consideration to stromal cell-derived factor-1(SDF1)that plays a vital role in tissue-specific migration of the cells needed for *** researchers recognized that SDF1 could facilitate site-specific migration of cells by regulating SDF1-CXCR4 and/or HMGB1-SDF1-CXCR4 pathways which is vital for tissue *** in this study,we have attempted to describe the role of different types of cells within the body in facilitating regeneration while emphasizing the HMGB1-SDF1-CXCR4 pathway that orchestrates the migration of cells to the site where regeneration is needed.

关键词： C-X-C motif chemokine 12 Mesenchymal stem cells Monocytes Neutrophils Peripheral blood mononuclear cells Receptor for advanced glycation end products

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Engineering catalytic efficiency of thermophilic lipase from <i>Geobacillus zalihae</i>by hydrophobic residue mutation near the catalytic pocket

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Advances in Bioscience and Biotechnology 2012年第2期3卷 158-167页

作者： Roswanira Abdul Wahab Mahiran Basri Mohd Basyaruddin Abdul Rahman Raja noor Zaliha Raja Abdul Rahman abu bakar Salleh Leow Thean Chor 3Institute of Bioscience Universiti Putra Malaysia Serdang Malaysia 4Faculty of Biotechnology and Biomolecular Sciences Universiti Putra Malaysia Serdang Malaysia Faculty of Science Universiti Putra Malaysia Serdang Malaysia Faculty of Science Universiti Teknologi Malaysia Skudai Malaysia

In-silico and experimental investigations were conducted to explore the effects of substituting hydrophobic residues;Val, Met, Leu, Ile, Trp and Phe into the oxyanion Q114 of T1 lipase. We hypothesized that the oxyanion Q114, involved in substrate binding is also associated with modulation of conformational stability and in conferring specific enzyme attributes. The insilico investigations accurately predicted the quality of the protein packing in some of the variants. Our study found by altering the hydrophobicity of the oxyanion 114, remarkably altered enzyme conformational stability and catalytic attributes. Substitution with Leu resulted improvements in four out of the six tested characteristics. The hydrophobic Leu might have improved local structure folding and increased hydrophobic interactions with other residues in the vicinity of the mutation. The Met variant showed higher activity over the wild-type in hydrolyzing a wider range of natural oils. The bulky amino acids, Phe and Trp negatively affected T1 lipase and resulted in the largest disruption of protein stability and inferior enzyme characteristics. We have successfully illustrated that a single point residue changes at oxyanion 114 could result in a myriad of enzyme attributes, which implied there was some interplay between hydrophobicity and conformation for lipase catalytic functions.

关键词： Hydrophobic Residue T1 Lipase Oxyanion Conformational Stability Catalytic Pocket

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Identifying and Prioritizing the Research Needs Related to Mental Health in Gaza Strip, Palestine

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Open Journal of Psychiatry 2015年第1期5卷 19-25页

作者： Nasser Ibrahim abu-El-noor Yousef Ibrahim Aljeesh College of Nursing Islamic University of Gaza Gaza Palestine

Background: An important function of research is to identify community needs in certain fields. As mental health is a vital issue to us, identifying and prioritizing mental health needs is important to policy makers to help them in setting goals for different programs that meet the needs of a specific community in a certain health area. Purpose: To identify the health research needs in the field of?mental health in Gaza Strip, Palestine. Design and Methods: The design was a qualitative design using?focus group, need assessment and non-structured interview for data collection. Participants: Participants included three focus groups. First group consisted of students enrolled in a master program?of community mental health nursing;second group included seven faculty members who were specialists?in mental health;and third group included six key persons from the ministry of health and?the chair of community mental health program in WHO at Gaza Strip. Results: Results of this qualitative?study revealed several themes that emerged from the thematic analysis. Many health research?needs were identified by participants. These needs included the following topics: stigma, family integration, aggression of children born to substance-abuse fathers, post-partum depression, counseling, talking therapy in clinical practice, behavioral problems, tramadole abuse, risk factors leadingto drug dependence, autism, exploring the role of religion in mental health, and Wellness Recovery Action Plan. Implication for Practice: Well designed studies will help to identify and prioritize the health needs for a specific community. Identifying the needs related to community mental health?is one of the first steps to help in pushing these needs into the agenda of health policy makers, who then will work to set goals and design policies and programs that aiming to meet the needs of the community, which hopefully will produce a community with less mental health problems.

关键词： Research Needs Research Priorities Mental Health Focus Group Gaza Strip

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