检索结果-南通市图书馆

Mechanism studies of the activation of DNA methyltransferase DNMT1 triggered by histone H3 ubiquitination,revealed by multi-scale molecular dynamics simulations

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2023年第2期66卷 313-323页

作者： Jixue Sun Fei Liu Longxiao Yuan ning-ning pang Bing Zhu Na Yang State Key Laboratory of Medicinal Chemical Biology College of Pharmacy and Key Laboratory of Medical Data Analysis and Statistical Research of TianjinNankai UniversityTianjin 300353China National Laboratory of Biomacromolecules CAS Center for Excellence in BiomacromoleculesInstitute of BiophysicsChinese Academy of SciencesBeijing 100101China

DNMT1 is a DNA methyltransferase that catalyzes and maintains methylation in CpG *** blocks the entrance of DNA into the catalytic pocket via the replication foci targeting sequence(RFTS)*** studies have shown that an H3-tail-conjugated two-mono-ubiquitin mark(H3Ub2)activates DNMT1 by binding to the RFTS ***,the activation mechanism of DNMT1 remains *** this work,we combine various sampling methods of extensive simulations,including conventional molecular dynamics,Gaussian-accelerated molecular dynamics,and coarse-grained molecular dynamics,to elucidate the activation mechanism of *** and energy analyses show that binding of H3Ub2 to the RFTS domain of DNMT1 results in the bending of theα4-helix in the RFTS domain at approximately 30°–35°,and the RFTS domain rotates~20°anti-clockwise and moves~3?away from the target recognition domain(TRD).The hydrogen-bonding network at the RFTSTRD interface is significantly disrupted,implying that the RFTS domain is dissociated from the catalytic core,which contributes to activating the auto-inhibited conformation of *** results provide structural and dynamic evidence for the role of H3Ub2 in regulating the catalytic activity of DNMT1.

关键词： DNA methyltransferase conformational change molecular dynamics simulation activation molecular mechanism

Highly active cobalt-doped nickel sulfide porous nanocones for high-performance quasi-solid-state zinc-ion batteries

在线全文

学校读者我要写书评

暂无评论

Journal of Energy Chemistry 2022年第3期31卷 237-249,I0008页

作者： Xin Tong Yun Li ning pang Yang Zhou Dajun Wu Dayuan Xiong Shaohui Xu Lianwei Wang Paul K.Chu Key Laboratory of Polar Materials and Devices(MOE) Department of ElectronicsEast China Normal UniversityShanghai 200241China Jiangsu Laboratory of Advanced Functional Materials School of Electronic and Information EngineeringChangshu Institute of TechnologyChangshu 215500JiangsuChina School of Physics and Electronic Engineering Hanshan Normal UniversityChaozhou 521041GuangdongChina Department of Physics Department of Materials Science and Engineeringand Department of Biomedical EngineeringCity University of Hong KongKowloon 999077Hong KongChina

Flexible quasi-solid zinc-ion batteries(ZIBs)have large potential in power applications due to the low price,wearable nature,safety,and high ***,the use of transition metal sulfide cathodes in ZIBs has not been studied extensively and the underlying mechanism and theoretical basis of this type of batteries are not well ***,a highly active cobalt-doped Ni_(3)S_(2) porous nanocone framework(C12NS)is designed and demonstrated as a zinc-ion battery ***-principles calculation and experiments reveal that the cobalt dopant improves the battery properties *** assembled flexible zinc-ion battery exhibits a high specific capacity of 453.3 mAh g^(−1)at a current density of 0.4 A g^(−1)in as well as excellent cycling stability as manifested by a capacity retention ratio of 89.5%at a current density of 4 A g^(−1)after 5000 *** peak energy density of 553.9 Wh kg^(−1)is also superior to those of most recently reported NiCo-based zinc-ion *** importantly,the flexible battery can be operated under severe mechanical bending and even continues to work after physical puncturing without showing *** exciting results not only reveal a novel design of cathode materials for zinc-based batteries,but also suggest their immense commercial potential in portable and wearable electronics.

关键词： Zn-ion battery Quasi-solid electrolyte Nanocone Doping Wearable electronic

Design and Research of a Portable Tool for Assisting Elderly People Walking

在线全文

学校读者我要写书评

暂无评论

International Journal of Plant Engineering and Management 2022年第1期27卷 39-52页

作者： WU Daikun pang ning Xi′an Jiaotong Engineering Institute Xi′an 710300China

Based on the serious aging of our country′s population,starting from the impact on the quality of life of the elderly,this paper first analyzes the factors affecting the lives of the elderly,and makes predictions based on my country′s elderly care industry *** research,a portable tool for assisting elderly people walking is designed,in which electric drive,steering mechanism and seat functions are added by ordinary auxiliary walking *** can sit down and ride when they are tired,mainly including seats and walking *** characteristics of its users and various influencing factors are *** research results can guide the solution of the current portable tools that assist the elderly in walking.

关键词： crutches electric drives steering mechanisms portable

在线全文

学校读者我要写书评

暂无评论

儿童慢性肾脏病概述

中华儿科杂志 2010年第5期48卷 361-364页

作者：庞宁卫生部中友好医院儿科北京100029

一、儿童慢性肾脏病的概况儿童的慢性肾脏病（CKD）常进展至终末期肾病（ESRD），所以如何延缓CKD的进程备受关注。世界范围CKD病例不断增多，成人CKD已被认为是公众健康的主要问题。

关键词：儿童慢性肾脏病肾功能衰竭概述肾小球肾炎

A broadband achromatic metalens array for integral imaging in the visible

在线全文

学校读者我要写书评

暂无评论

Light(Science & Applications) 2019年第1期8卷 586-595页

作者： Zhi-Bin Fan Hao-Yang Qiu Han-Le Zhang Xiao-ning pang Li-Dan Zhou Lin Liu Hui Ren Qiong-Hua Wang Jian-Wen Dong State Key Laboratory of Optoelectronic Materials and Technologies Sun Yatsen UniversityGuangzhou 510275China School of Physics Sun Yat-sen UniversityGuangzhou 510275China School of Instrumentation and Optoelectronic Engineering Beihang UniversityBeijing 100191China

Integral imaging is a promising three-dimensional(3D)imaging technique that captures and reconstructs light field *** arrays are usually used for the reconstruction process to display 3D scenes to the ***,the inherent chromatic aberration of the microlens array reduces the viewing quality,and thus,broadband achromatic imaging remains a challenge for integral ***,we realize a silicon nitride metalens array in the visible region that can be used to reconstruct 3D optical scenes in the achromatic integral imaging for white *** metalens array contains 60×60 polarization-insensitive metalenses with nearly diffraction-limited *** nanoposts in each high-efficiency(measured as 47%on average)metalens are delicately designed with zero effective material dispersion and an effective achromatic refractive index distribution from 430 to 780 *** addition,such an achromatic metalens array is composed of only a single silicon nitride layer with an ultrathin thickness of 400 nm,making the array suitable for on-chip hybrid-CMOS integration and the parallel manipulation of optoelectronic *** expect these findings to provide possibilities for full-color and aberration-free integral imaging,and we envision that the proposed approach may be potentially applicable in the fields of high-power microlithography,high-precision wavefront sensors,virtual/augmented reality and 3D imaging.

关键词： chromatic integral visible

Culture and Identification of Human Amniotic Mesenchymal Stem Cells

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Medical Sciences Journal 2010年第4期25卷 211-214页

作者： Shuang-zhi Huo Ping Shi Xi-ning pang Department of Stem Cells and Regenerative Medicine Key Laboratory of Cell Biology of Ministry of Health China Medical University Shenyang 110001 China

Objective To establish the method of isolation, purification, and identification of human amniotic mesenchymal stem cells (hAMSCs). Methods hAMSCs were isolated from human amniotic membrane by trypsin-collagenase digestion, and cultured in Dulbecco's modified Eagle's medinm/F12 medium supplemented with 10% fetal bovine serum. Phenotypic characteristics of these cells were analyzed by means of immunocytochemistry and flow cytometry. Results The cells successfully isolated from human amniotic membrane expressed representative mesenchymal cell surface markers CD44, CD90, and vimentin, but not CD45. Conclusions This study establishes a potential method for isolation of hAMSCs from human amnion, in vitro culture, and identification. The isolated cells show phenotypic characteristics of mesenchymal stem cells.

关键词： amniotic mesenchymal stem cell cell isolation cell culture cell identification

在线全文

学校读者我要写书评

暂无评论

瘦素与肾脏和慢性肾功能衰竭

中华儿科杂志 2004年第6期42卷 474-476页

作者：庞宁周湘北京中日友好医院儿科 100029

Leptin 源自希腊语的Leptos,意思是"瘦的",它与肥胖的关系有很多研究. 瘦素(Leptin)是肥胖(Ob)基因的产物,是由167个氨基酸组成的多肽,源于脂肪组织分泌的激素,主要由白色脂肪细胞合成,释放入血中,并通过摄食、能量消耗来调节机体能量平... 详细信息

Leptin 源自希腊语的Leptos,意思是"瘦的",它与肥胖的关系有很多研究. 瘦素(Leptin)是肥胖(Ob)基因的产物,是由167个氨基酸组成的多肽,源于脂肪组织分泌的激素,主要由白色脂肪细胞合成,释放入血中,并通过摄食、能量消耗来调节机体能量平衡,通过中枢与末梢组织中的受体介导起作用,作为脂肪组织的信使,其作用于下丘脑部,瘦素抑制神经肽Y而有减低食欲的作用.瘦素与下丘脑-垂体-性腺轴的活性也有关[1-4].

关键词：瘦素肾脏疾病慢性肾功能衰竭肾移植腹膜透析肾替代疗法

Tumour length is an independent prognostic factor of esophageal squamous cell carcinomas

在线全文

学校读者我要写书评

暂无评论

Chinese Medical Journal 2012年第24期125卷 4445-4448页

作者： WU ning pang Lie-wen CHEN Zhi-ming MA Qin-yun CHEN Gang Department of Cardiothoracic Surgery Huashan Hospital Fudan University Shanghai 200040 China

Background The latest version of the American Joint Committee on Cancer （AJCC） TNM staging system has not comprehensively evaluated the impact of tumour length on survival in patients with esophageal squamous cell carcinoma. Our study explored the relationship between tumour length and clinicopathological characteristics as well as long-term survival. Methods All 202 cases of esophageal resections done from January 1, 2004 to December 31, 2008 in Huashan Hospital, Fudan University were reviewed and followed up. Results Patients with tumour length 〉3 cm were related to more advanced tumour stage （χ2=55.9, P 〈0.001）, more metastatic lymph nodes （χ2=14.6, P 〈0.001）, increased metastatic lymph node ratio （χ2=16.1, P 〈0.001） and worse overall TNM stage （χ2=48.1, P 〈0.001）. Univariate and multivariate analyses indicated that tumour length was a significant prognostic risk factor （95% CI 0.235-0.947, P=-0.035）. Subgroup analyses disclosed that tumour length was a valuable prognostic predictor in patients with lower T stage, absence of metastatic lymph nodes and lower TNM stage. Conclusions Esophageal tumour length is a predictive factor for long-term survival especially for lower tumour stage, absence of metastatic lymph nodes and lower TNM stage patients. Tumour length should be incorporated in the staging system as an important grouping factor for better prognostic evaluation.

关键词： esophageal squamous cell carcinoma tumour length prognosis

在线全文

学校读者我要写书评

暂无评论

中国传统节日与外国留学生教育

当代青年研究 2007年第7期 32-36页

作者：房宁上海交通大学

中国传统节日具有深刻的思想内涵,开展中国传统节日教育对于外国留学生的汉语学习、进一步了解中国、增进与中国人民的交流与友谊都有积极的促进作用,理应在汉语国际推广中受到重视。

关键词：中国传统节日留学生文化教育