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Genome-Wide Exploration of the Grape GLR Gene Family and Differential Responses of VvGLR3.1 and VvGLR3.2 to Low Temperature and Salt Stress

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Phyton-International Journal of Experimental Botany 2024年第3期93卷 533-549页

作者： Honghui Sun Ruichao liu Yueting Qi Hongsheng Gao Xueting Wang ning Jiang Xiaotong guo Hongxia Zhang Chunyan Yu The Engineering Research Institute of Agriculture and Forestry Ludong UniversityYantai264025China College of Agriculture Ludong UniversityYantai264025China

Grapes,one of the oldest tree species globally,are rich in ***,environmental conditions such as low temperature and soil salinization signiﬁcantly affect grape yield and *** glutamate receptor(GLR)family,comprising highly conserved ligand-gated ion channels,regulates plant growth and development in response to *** this study,11 members of the VvGLR gene family in grapes were identiﬁed using whole-genome sequence *** methods were employed to analyze the basic physical and chemical properties,phylogenetic trees,conserved domains,motifs,expression patterns,and evolutionary *** and collinear analyses revealed that the VvGLRs were divided into three subgroups,showing the high conservation of the grape GLR *** members exhibited 2 glutamate receptor binding regions(GABAb and GluR)and 3-4 transmembrane regions(M1,M2,M3,and M4).Real-time quantitative PCR analysis demonstrated the sensitivity of all VvGLRs to low temperature and salt *** localization studies in Nicotiana tabacum veriﬁed that VvGLR3.1 and VvGLR3.2 proteins were located on the cell membrane and cell ***,yeast transformation experiments conﬁrmed the functionality of VvGLR3.1 and VvGLR3.2 in response to low temperature and salt ***ﬁndings highlight the signiﬁcant role of the GLR family,a highly conserved group of ion channels,in enhancing grape stress *** study offers new insights into the grape GLR gene family,providing fundamental knowledge for further functional analysis and breeding of stress-resistant grapevines.

关键词： Genome-wide identiﬁcation glutamate receptor(GLR)family low temperature stress salt stress grape

Fast Two-dimensional Positioning Method of Crab Pulsar Based on Multiple Optimization Algorithms

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Research in Astronomy and Astrophysics 2024年第10期24卷 92-103页

作者： Jianfeng Chen Jin liu Xin Ma Xiaolin ning College of Information Science and Engineering Wuhan University of Science and TechnologyWuhan 430081China School of Instrumentation Science and Opto-electronics Engineering Beihang UniversityBeijing 100191China Hefei National Laboratory Hefei 230088China

In the two-dimensional positioning method of pulsars, the grid method is used to provide non-sensitive direction and positional estimates. However, the grid method has a high computational load and low accuracy due to the interval of the grid. To improve estimation accuracy and reduce the computational load, we propose a fast twodimensional positioning method for the crab pulsar based on multiple optimization algorithms(FTPCO). The FTPCO uses the Levenberg–Marquardt(LM) algorithm, three-point orientation(TPO) method, particle swarm optimization(PSO) and Newton–Raphson-based optimizer(NRBO) to substitute the grid method. First, to avoid the influence of the non-sensitive direction on positioning, we take an orbital error and the distortion of the pulsar profile as optimization objectives and combine the grid method with the LM algorithm or PSO to search for the non-sensitive direction. Then, on the sensitive plane perpendicular to the non-sensitive direction, the TPO method is proposed to fast search the sensitive direction and sub-sensitive direction. Finally, the NRBO is employed on the sensitive and sub-sensitive directions to achieve two-dimensional positioning of the Crab pulsar. The simulation results show that the computational load of the FTPCO is reduced by 89.4% and the positioning accuracy of the FTPCO is improved by approximately 38% compared with the grid method. The FTPCO has the advantage of high real-time accuracy and does not fall into the local optimum.

关键词： (stars:)pulsars:individual(Crab pulsar) space vehicles methods:data analysis astrometry

Safety for all temperature zinc-ion batteries

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Journal of Energy Chemistry 2024年第7期94卷 299-301页

作者： Sailin liu Zaiping guo School of Chemical Engineering the University of AdelaideSA 5000Australia

Zinc-ion batteries(ZIBs)have emerged as a promising candidate in the grid scale energy storage,offering an alternative to conventional lithium-ion ***,as research and development efforts in this field progress,it becomes increasingly important to address potential hazards associated with flammable gases and electrolyte *** future paper aims to raise awareness of safety issues that may be overlooked and underscore two primary temperature-induced hazards:long-term cycling H_(2) gas accu-mulation and flammable organic *** evaluate various electrolytes aimed at minimizing gas pro-duction and improving high-temperature ***,we emphasize the effectiveness of electrolyte modifications,especially co-solvent approach in addressing the above safety concerns and suggest pathways for formulating practical safe ***,apart from examining the inherent electrochemical stability window(EsW)of the electrolyte,we urge consideration of side reaction prod-ucts on electrode interfaces and high temperatures as factors influencing a battery's EsW.

关键词： battery electrolyte offering

A New High-Precision and Lightweight Detection Model for Illegal Construction Objects Based on Deep Learning

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Tsinghua Science and Technology 2024年第4期29卷 1002-1022页

作者： Wenjin liu Lijuan Zhou Shudong Zhang ning Luo Min Xu School of Cyberspace Security(School of Cryptology) Hainan UniversityHaikou 570228China School of Information Engineering Capital Normal UniversityBeijing 100048China

Illegal construction has caused serious harm around the world. However, current methods are difficult to detect illegal construction activities in time, and the calculation complexity and the parameters of them are large. To solve these challenges, a new and unique detection method is proposed, which detects objects related to illegal buildings in time to discover illegal construction activities. Meanwhile, a new dataset and a high-precision and lightweight detector are proposed. The proposed detector is based on the algorithm You Only Look Once (YOLOv4). The use of DenseNet as the backbone of YDHNet enables better feature transfer and reuse, improves detection accuracy, and reduces computational costs. Meanwhile, depthwise separable convolution is employed to lightweight the neck and head to further reduce computational costs. Furthermore, H-swish is utilized to enhance non-linear feature extraction and improve detection accuracy. Experimental results illustrate that YDHNet realizes a mean average precision of 89.60% on the proposed dataset, which is 3.78% higher than YOLOv4. The computational cost and parameter count of YDHNet are 26.22 GFLOPs and 16.18 MB, respectively. Compared to YOLOv4 and other detectors, YDHNet not only has lower computational costs and higher detection accuracy, but also timely identifies illegal construction objects and automatically detects illegal construction activities.

关键词： object detection lightweight illegal buildings illegal construction objects high-precision

Improvement of the piezoelectricity of PVDF-HFP by CoFe_(2)O_(4)nanoparticles

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Nano Materials Science 2024年第2期6卷 201-210页

作者： Dan Lei ning Hu Liangke Wu Alamusi Huiming ning Yang Wang Zhaonan Jin Yaolu liu College of Aerospace Engineering Chongqing UniversityChongqing400044China State Key Laboratory of Reliability and Intelligence Electrical Equipment School of Mechanical EngineeringHebei University of TechnologyTianjin300401China

High piezoelectric composite films composed of poly(vinylidene fluoride-co-hexafluoropropylene)(PVDF-HFP)and ferromagnetic cobalt ferrite(CoFe_(2)O_(4))(0.00 wt%to 0.2 wt%)are prepared by a solution casting method accompanied by uniaxial stretching and high electric field *** decisive effect of the poling electric field on the power generating capability was confirmed by the *** pure PVDF-HFP films,when the maximum electric field Emax is 120 MV/m,the calibrated open circuit voltage reaches 2.93 V,which is much higher than those poled at lower electric fields(70 MV/m:1.41 V;90 MV/m:2.11 V).Furthermore,the addition of CoFe_(2)O_(4)also influences the piezoelectricity *** the samples containing 0.15 wt%CoFe_(2)O_(4),the calibrated open circuit voltage increases to the maximum value of 3.57 ***,the relative fraction of theβ-phase and the crystallinity degree are 99%and 48%,*** effects of CoFe_(2)O_(4)nanoparticles on initial crystallization,uniaxial stretching and high electric field poling are investigated by XRD,FTIR and DSC.

关键词： PVDF-HFP CoFe_(2)O_(4)nanoparticles Piezoelectricity Nanocomposite

Microstructure and texture evolution of Cu-Cr-Co-Ti alloys during the two-stage cryorolling

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Rare Metals 2024年第9期43卷 4535-4547页

作者： Jia-Jun Zhang Long-Jian Li Zhi-Feng liu Zong-ning Chen En-Yu guo Hui-Jun Kang Ren-Geng Li Tong-Min Wang Key Laboratory of Solidification Control and Digital Preparation Technology(Liaoning Province) School of Materials Science and EngineeringDalian University of TechnologyDalian 116024China Center of Hyper-Gravity Experimental and Interdisciplinary Research Zhejiang UniversityHangzhou 310058China ningbo Institute of Dalian University of Technology Ningbo 315000China Key Laboratory for Light-Weight Materials Nanjing Tech UniversityNanjing 210009China

This study entailed an investigation of the mechanical properties,microstructural and texture orientation evolutions of Cu-Cr-Co-Ti alloys prepared via twostage cryorolling and intermediate aging *** this end,X-ray diffraction and electron backscatter diffraction were *** results indicate that the two-stage cryorolling and intermediate aging treatments led to the development of profuse twin bundles and significantly enhanced the mechanical *** initial cryorolling led to coplanar slip and developed a strong Y({111})orientation,accelerating the formation of Goss({011})orientation and a Brass-type *** intermediate aging treatment relieved the restriction on dislocation slip and reoriented the grains toward the Copper({112})and Z({111})*** Z orientation,with a relatively high volume fraction,dominated the *** cryorolling intensified twinning and shear banding,transforming the Copper-type shear bands into Brass-type shear bands with rhomboidal prism *** areas inside the Brasstype shear bands exhibited a Y orientation,and the areas outside the shear bands exhibited a stable Brass-type *** evident decrease in the weighted Schmid factors demonstrated that the two-stage cryorolling and intermediate aging treatment can modify the texture evolution and aid the design of high-performance Cu alloys.

关键词： Copper alloys Two-stage cryorolling Macrotexture evolution Electron backscatter diffraction Schmid factor

Formation and properties of a Zr-based amorphous coating by laser cladding

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Rare Metals 2024年第4期43卷 1809-1814页

作者： ning Zhang Ying liu Wei Yang Shu-Jie Pang Key Laboratory of Aerospace Materials and Performance(Ministry of Education) School of Materials Science and EngineeringBeihang UniversityBeijing100191China

(Zr_(0.53)Al_(0.1)Ni_(0.05)Cu_(0.3)Ti_(0.02))_(99)Y_1(at%)coating with amorphous layer of about 180μm thick was prepared on a steel substrate by using laser cladding *** coating is compact and shows good metallurgical bonding with *** microstructure,microhardness and corrosion behavior along the depth from the coating surface to the substrate were *** is found that a gradient structure consisted of amorphous surface layer,amorphous-crystalline transitional layer and substrate is formed after the laser *** microhardness and corrosion behavior exhibit variation with the microstructural evolution at different depths from the coating *** microhardness and corrosion resistance in 3.5 wt%NaCl solution of the amorphous surface layer are comparable to those of the as-cast Zr-based BMG with the same composition,and higher than those of the steel substrate.

关键词： Laser cladding Amorphous coating Microstructure Hardness Corrosion resistance

Homoclinic Solutions for a Class of Perturbed Fractional Hamiltonian Systems with Subquadratic Conditions

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Acta Mathematica Sinica,English Series 2024年第5期40卷 1177-1196页

作者： Ying LUO Fei guo Yan liu School of Mathematics Tianjin UniversityTianjin 300354P.R.China Tianjin Key Laboratory of Brain-Inspired Intelligence Technology Tianjin300354P.R.China

In this paper,we consider the following perturbed fractional Hamiltonian systems{tD_(∞)^(α)(_(-∞)D_(t)^(α)u(t))+L(t)u(t)=■_(u)W(t,u(t))+■(u)G(t,u(t)),t∈R,u∈H^(α)(R,R^(N)),whereα∈(1/2,1],L∈C(R,R^(N×N))is symmetric and not necessarily required to be positive definite,W∈C1(R×R^(N,R))is locally subquadratic and locally even near the origin,and perturbed term G∈C1(R×R^(N,R))maybe has no parity in *** the perturbed method improved by the authors,a sequence of nontrivial homo clinic solutions is obtained,which generalizes previous results.

关键词： Perturbed fractional Hamiltonian systems subquadratic condition perturbed method homoclinic solutions multiplicity

Effect of solvent on the initiation mechanism of living anionic polymerization of styrene:A computational study

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Chinese Journal of Chemical Engineering 2024年第5期69卷 135-142页

作者： Shen Li Yin-ning Zhou Zhong-Xin liu Zheng-Hong Luo School of Chemical Engineering and Technology Hainan UniversityHaikou 570228China Department of Chemical Engineering School of Chemistry and Chemical EngineeringState Key Laboratory of Metal Matrix CompositesShanghai Jiao Tong UniversityShanghai 200240China

For living anionic polymerization(LAP),solvent has a great influence on both reaction mechanism and *** this work,by using the classical butyl lithium-styrene polymerization as a model system,the effect of solvent on the mechanism and kinetics of LAP was revealed through a strategy combining density functional theory(DFT)calculations and kinetic *** terms of mechanism,it is found that the stronger the solvent polarity,the more electrons transfer from initiator to solvent through detailed energy decomposition analysis of electrostatic interactions between initiator and solvent ***,we also found that the stronger the solvent polarity,the higher the monomer initiation energy barrier and the smaller the initiation rate ***,initiation is more favorable at lower temperatures based on the calculated results ofΔG_(TS).Finally,the kinetic characteristics in different solvents were further examined by kinetic *** is found that in benzene and n-pentane,the polymerization rate exhibits first-order ***,slow initiation and fast propagation were observed in tetrahydrofuran(THF)due to the slow free ion formation rate,leading to a deviation from first-order kinetics.

关键词： Living anionic polymerization Solvent effect Reaction kinetics Computational chemistry Mathematical modeling Kinetic modeling